MD09G1261400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015869
Physical Location & Seq
Forward (+)
33450242 .. 33452834
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1261400.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGGGAACACAATTGGAGGAAGAAGGAAAGCAAAGGTAATGAAAATCAGCGGGGAGACCTTCAAAGTAAAGACCCCGATAAGGGCATGGGACGTAGTGAAGGACTACCCAGGTCATGTCCTATTGAACTCAGAAGCTGTGAAACGTTTTGGCGTGAGGGCCAGGCCACTGGAACCACAAGAAGAATTGAAGCCCAAGAAAATATATTTTCTTGTCCAACTCCCCAAGTTTCCGGCGGAGGAAGAAGAAAAACAAGTGACAAGAAGAGTGCGGTCGGGTATTCAAGTGAGTGCCAAGGACAGGCTCGAGTGCTTGATGCTTTCACGGCGGTCGGTGTCGGATCTTACGAGTGTTAGGGCATCGTCGACAACGTCGAGTTCATCATCGGAGGGACCGGTGCGGATGAAGATGAGGCTGCCGAAGGCTCAAATGGTAAAACTGGTTGAAGAGAGTAAGGATGACGTGGAAATTGCTGAGAGGATAATAGAGCTTTATATGGTTAATAATGGTGGAAAGGTCGAAGGGCAGATGGTGGAGAAGGAAGAAGGCGTTTTGCAGCGGAAGCGTGGTGGTGGCAACCTCAAGCCGAGTCATGAGCGAGTGAGTTTTGTCCCTGTGGAAGAAGAAGAAATTCGTTTAGATCTTGCCCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

219

Amino Acids

24.63

Weight (kDa)

9.62

Isoelectric Point (pI)

64.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 180 8.4e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 365
AciI CCGC 6 cut(s) 51, 236, 271, 328, 400, 559
AclI AACGTT 1 cut(s) 145
AclWI GGATC 1 cut(s) 348
AcsI RAATTY 1 cut(s) 630
AfiI CCNNNNNNNGG 3 cut(s) 81, 82, 300
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 64, 127, 190, 284, 446
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 109, 161
AluBI AGCT 2 cut(s) 137, 490
AluI AGCT 2 cut(s) 137, 490
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 1 cut(s) 348
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 159, 164
ApeKI GCWGC 2 cut(s) 415, 556
ApoI RAATTY 1 cut(s) 630
AsiGI ACCGGT 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 630
AspS9I GGNCC 2 cut(s) 159, 392
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 402, 568
BccI CCATC 1 cut(s) 523
BceAI ACGGC 1 cut(s) 341
BciT130I CCWGG 2 cut(s) 111, 163
BcoDI GTCTC 1 cut(s) 50
BglII AGATCT 1 cut(s) 640
BisI GCNGC 2 cut(s) 416, 557
BlsI GCNGC 2 cut(s) 417, 558
Bme1390I CCNGG 2 cut(s) 111, 163
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 305
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 2 cut(s) 159, 392
BmiI GGNNCC 2 cut(s) 174, 393
BmrFI CCNGG 2 cut(s) 111, 163
BmsI GCATC 2 cut(s) 306, 368
BpuEI CTTGAG 1 cut(s) 566
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 109, 294
BsaWI WCCGGW 1 cut(s) 394
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 82, 300
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 174, 444
BseBI CCWGG 2 cut(s) 111, 163
BseDI CCNNGG 2 cut(s) 109, 294
BseGI GGATG 2 cut(s) 408, 463
BseLI CCNNNNNNNGG 3 cut(s) 81, 82, 300
BseMII CTCAG 2 cut(s) 144, 465
BseNI ACTGG 2 cut(s) 174, 444
BseXI GCAGC 2 cut(s) 402, 568
Bsh1285I CGRYCG 2 cut(s) 275, 332
BshFI GGCC 2 cut(s) 161, 166
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 2 cut(s) 275, 332
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 233, 395
BslFI GGGAC 3 cut(s) 104, 405, 596
BslI CCNNNNNNNGG 3 cut(s) 81, 82, 300
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 3 cut(s) 104, 405, 596
BsnI GGCC 2 cut(s) 161, 166
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 305
Bsp143I GATC 2 cut(s) 340, 640
BspACI CCGC 6 cut(s) 51, 236, 271, 328, 400, 559
BspANI GGCC 2 cut(s) 161, 166
BspCNI CTCAG 2 cut(s) 143, 466
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 174, 393
BspPI GGATC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 2 cut(s) 174, 444
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 109, 294
BssMI GATC 2 cut(s) 340, 640
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 111, 163
Bst6I CTCTTC 2 cut(s) 259, 441
BstDEI CTNAG 2 cut(s) 130, 474
BstF5I GGATG 2 cut(s) 408, 463
BstKTI GATC 2 cut(s) 343, 643
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 2 cut(s) 340, 640
BstMCI CGRYCG 2 cut(s) 275, 332
BstMWI GCNNNNNNNGC 2 cut(s) 325, 562
BstNI CCWGG 2 cut(s) 111, 163
BstSCI CCNGG 2 cut(s) 109, 161
BstV1I GCAGC 2 cut(s) 402, 568
BstX2I RGATCY 2 cut(s) 340, 640
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 2 cut(s) 340, 640
BsuRI GGCC 2 cut(s) 161, 166
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 408, 463
BtsIMutI CAGTG 1 cut(s) 167
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 159, 392
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 3 cut(s) 87, 116, 593
CviJI RGCY 9 cut(s) 137, 161, 166, 193, 304, 415, 425, 490, 586
CviKI_1 RGCY 9 cut(s) 137, 161, 166, 193, 304, 415, 425, 490, 586
DdeI CTNAG 2 cut(s) 130, 474
DpnI GATC 2 cut(s) 342, 642
DpnII GATC 2 cut(s) 340, 640
Eam1104I CTCTTC 2 cut(s) 259, 441
EarI CTCTTC 2 cut(s) 259, 441
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 109, 161
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 90, 119, 596
FaiI YATR 6 cut(s) 88, 117, 205, 495, 497, 594
FaqI GGGAC 3 cut(s) 104, 405, 596
FatI CATG 3 cut(s) 86, 115, 592
FauI CCCGC 1 cut(s) 44
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 2 cut(s) 416, 557
FokI GGATG 2 cut(s) 415, 470
Fsp4HI GCNGC 2 cut(s) 416, 557
GluI GCNGC 2 cut(s) 416, 557
HaeIII GGCC 2 cut(s) 161, 166
HapII CCGG 2 cut(s) 233, 395
Hin1II CATG 3 cut(s) 90, 119, 596
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HinfI GANTC 1 cut(s) 589
HpaII CCGG 2 cut(s) 233, 395
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 3 cut(s) 133, 340, 388
Hpy188III TCNNGA 1 cut(s) 593
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 2 cut(s) 367, 376
HpyAV CCTTC 7 cut(s) 18, 70, 94, 415, 515, 532, 539
HpyCH4IV ACGT 4 cut(s) 93, 145, 371, 462
HpyCH4V TGCA 1 cut(s) 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 562
HpyF3I CTNAG 2 cut(s) 130, 474
HpySE526I ACGT 4 cut(s) 93, 145, 371, 462
Hsp92II CATG 3 cut(s) 90, 119, 596
Kzo9I GATC 2 cut(s) 340, 640
Lsp1109I GCAGC 2 cut(s) 402, 568
LweI GCATC 2 cut(s) 306, 368
MaeII ACGT 4 cut(s) 93, 145, 371, 462
MaeIII GTNAC 1 cut(s) 256
MalI GATC 2 cut(s) 342, 642
MboI GATC 2 cut(s) 340, 640
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 2 cut(s) 340, 640
MluCI AATT 4 cut(s) 12, 185, 468, 630
MlyI GAGTC 1 cut(s) 598
MmeI TCCRAC 2 cut(s) 241, 318
MnlI CCTC 7 cut(s) 11, 150, 232, 382, 405, 471, 590
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 51, 559
MspI CCGG 2 cut(s) 233, 395
MspR9I CCNGG 2 cut(s) 111, 163
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 111, 163
MwoI GCNNNNNNNGC 2 cut(s) 325, 562
NdeII GATC 2 cut(s) 340, 640
NlaIII CATG 3 cut(s) 90, 119, 596
NlaIV GGNNCC 2 cut(s) 174, 393
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 1 cut(s) 256
PaeR7I CTCGAG 1 cut(s) 305
PagI TCATGA 1 cut(s) 592
PcsI WCGNNNNNNNCGW 3 cut(s) 344, 368, 371
PdmI GAANNNNTTC 1 cut(s) 630
PflFI GACNNNGTC 1 cut(s) 370
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 2 cut(s) 417, 558
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 109, 161
PspGI CCWGG 2 cut(s) 109, 161
PspN4I GGNNCC 2 cut(s) 174, 393
PspPI GGNCC 2 cut(s) 159, 392
PspXI VCTCGAGB 1 cut(s) 305
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 340, 640
PsyI GACNNNGTC 1 cut(s) 370
SalI GTCGAC 1 cut(s) 364
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 2 cut(s) 416, 557
Sau3AI GATC 2 cut(s) 340, 640
Sau96I GGNCC 2 cut(s) 159, 392
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 111, 163
SfaNI GCATC 2 cut(s) 306, 368
Sfr274I CTCGAG 1 cut(s) 305
SinI GGWCC 1 cut(s) 392
SlaI CTCGAG 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 305, 581
SmoI CTYRAG 2 cut(s) 305, 581
Sse9I AATT 4 cut(s) 12, 185, 468, 630
SsiI CCGC 6 cut(s) 51, 236, 271, 328, 400, 559
StyD4I CCNGG 2 cut(s) 109, 161
StyI CCWWGG 1 cut(s) 294
TaiI ACGT 4 cut(s) 96, 148, 374, 465
TaqI TCGA 4 cut(s) 306, 365, 374, 519
TasI AATT 4 cut(s) 12, 185, 468, 630
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 2 cut(s) 415, 556
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 3 cut(s) 56, 369, 419
TspRI CASTG 1 cut(s) 174
Tth111I GACNNNGTC 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 1 cut(s) 630
XhoI CTCGAG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.