RLG00000022707

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015869
Physical Location & Seq
Reverse (-)
10189697 .. 10191737
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022707

Sequence Viewer

Length: 663 bp
ATGGGAAACACAATTGGAGGAAAAAGCAGGAAAGCAAAGGTGATGAAGATAAGTGGAGAAACGTTCAAGGTAAAGACTCCTATACGAGCTGGGGATGTAGTGAAGGAGCACCCAGGTCATATGCTATTGAATTCTGAAGCTGTCAACCATTTTGGTATGAGAGCTAAGCCATTAGAAGCGGAACAAGAGCTCAAGCCCAGGAGTATATATTTTCTGGTAGAATTGCCCAATTTTGATTCGGAAGAAGAAGCAGGAAGAAGAAGTAATAGCAGGGTGCCGAGAAGGGTGAGATCTTCGGCCATTATTCAAGTGAGCGCCAAGGAGAGGCTCGAGTGCTTGATGCTGTCTCGACGCTCTGCTTCCGACCTTACGAGTCTTGTGAGAGCAGCACCACCATCTTTAGGTGGAGCAGGAGGAGGAGGAGGAGCTCCGGTGAGGATGAAGATGAGGCTGCCGAGGGCGCAGGTGGTCAAATTGATGGAGGAGAGTAAGGATGAGATTGAAGTCGCCGACAAAATCATTCAGCTCTATATGGCCAATGCCGTCACCGTCGGCGATAACAGCAACATGGACAAAAACTTCACTTTCAAGGCGCGTCAGAAGCGAGTGAGTTTTGGCCCCGAGAAGAAAGGAGAAATTCAATTAGATGTAGCTCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

221

Amino Acids

24.22

Weight (kDa)

9.95

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 163 6.2e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 274
AccII CGCG 1 cut(s) 595
AciI CCGC 1 cut(s) 179
AclI AACGTT 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 297, 534
AcsI RAATTY 2 cut(s) 130, 636
AcuI CTGAAG 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 324, 401
AgsI TTSAA 6 cut(s) 67, 130, 308, 503, 589, 641
AjnI CCWGG 2 cut(s) 112, 197
AluBI AGCT 7 cut(s) 89, 140, 164, 190, 428, 526, 653
AluI AGCT 7 cut(s) 89, 140, 164, 190, 428, 526, 653
Alw21I GWGCWC 3 cut(s) 111, 192, 430
Alw26I GTCTC 1 cut(s) 351
Ama87I CYCGRG 2 cut(s) 329, 620
AoxI GGCC 3 cut(s) 297, 534, 616
ApeKI GCWGC 2 cut(s) 386, 451
ApoI RAATTY 2 cut(s) 130, 636
AspLEI GCGC 3 cut(s) 317, 463, 595
AspS9I GGNCC 1 cut(s) 617
AsuHPI GGTGA 4 cut(s) 52, 298, 445, 538
AvaI CYCGRG 2 cut(s) 329, 620
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 1 cut(s) 274
BanII GRGCYC 2 cut(s) 192, 430
Bbv12I GWGCWC 3 cut(s) 111, 192, 430
BbvI GCAGC 2 cut(s) 398, 438
BccI CCATC 2 cut(s) 403, 472
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 2 cut(s) 114, 199
BcoDI GTCTC 1 cut(s) 351
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 454
BglII AGATCT 1 cut(s) 290
BisI GCNGC 2 cut(s) 387, 452
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 2 cut(s) 388, 453
Bme1390I CCNGG 2 cut(s) 114, 199
BmeT110I CYCGRG 2 cut(s) 329, 620
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 276, 619
BmrFI CCNGG 2 cut(s) 114, 199
BmsI GCATC 1 cut(s) 330
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 112, 197, 318, 455
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 401
BseBI CCWGG 2 cut(s) 114, 199
BseDI CCNNGG 4 cut(s) 112, 197, 318, 455
BseGI GGATG 3 cut(s) 100, 444, 499
BseLI CCNNNNNNNGG 2 cut(s) 324, 401
BseRI GAGGAG 6 cut(s) 429, 432, 435, 438, 497, 645
BseXI GCAGC 2 cut(s) 398, 438
BseYI CCCAGC 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 3 cut(s) 299, 536, 618
BshNI GGYRCC 1 cut(s) 274
BsiHKAI GWGCWC 3 cut(s) 111, 192, 430
BsiHKCI CYCGRG 2 cut(s) 329, 620
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 2 cut(s) 324, 401
BsmAI GTCTC 1 cut(s) 351
BsnI GGCC 3 cut(s) 299, 536, 618
BsoBI CYCGRG 2 cut(s) 329, 620
Bsp1286I GDGCHC 3 cut(s) 111, 192, 430
Bsp143I GATC 1 cut(s) 290
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 1 cut(s) 179
BspANI GGCC 3 cut(s) 299, 536, 618
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 276, 619
BspMI ACCTGC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 274
BssECI CCNNGG 4 cut(s) 112, 197, 318, 455
BssMI GATC 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 2 cut(s) 114, 199
Bst4CI ACNGT 1 cut(s) 550
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 100, 444, 499
BstFNI CGCG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 3 cut(s) 317, 463, 595
BstKTI GATC 1 cut(s) 293
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 1 cut(s) 290
BstMWI GCNNNNNNNGC 3 cut(s) 460, 561, 601
BstNI CCWGG 2 cut(s) 114, 199
BstSCI CCNGG 2 cut(s) 112, 197
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 398, 438
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 3 cut(s) 299, 536, 618
BtsCI GGATG 3 cut(s) 100, 444, 499
BveI ACCTGC 1 cut(s) 454
CfoI GCGC 3 cut(s) 317, 463, 595
Cfr13I GGNCC 1 cut(s) 617
CseI GACGC 2 cut(s) 360, 584
CviAII CATG 1 cut(s) 568
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 292
DpnII GATC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 297, 534
Ecl136II GAGCTC 2 cut(s) 190, 428
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 2 cut(s) 192, 430
Eco53kI GAGCTC 2 cut(s) 190, 428
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 2 cut(s) 329, 620
EcoICRI GAGCTC 2 cut(s) 190, 428
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 112, 197
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 2 cut(s) 192, 430
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 1 cut(s) 571
FaiI YATR 9 cut(s) 83, 120, 122, 158, 206, 208, 531, 533, 569
FatI CATG 1 cut(s) 567
FauNDI CATATG 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 387, 452
FokI GGATG 3 cut(s) 107, 451, 506
FriOI GRGCYC 2 cut(s) 192, 430
Fsp4HI GCNGC 2 cut(s) 387, 452
GlaI GCGC 3 cut(s) 316, 462, 594
GluI GCNGC 2 cut(s) 387, 452
GsaI CCCAGC 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 3 cut(s) 299, 536, 618
HapII CCGG 1 cut(s) 431
HgaI GACGC 2 cut(s) 360, 584
HhaI GCGC 3 cut(s) 317, 463, 595
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 3 cut(s) 315, 461, 593
HinP1I GCGC 3 cut(s) 315, 461, 593
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 3 cut(s) 76, 236, 373
HpaII CCGG 1 cut(s) 431
HphI GGTGA 4 cut(s) 52, 298, 445, 538
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 4 cut(s) 136, 241, 364, 600
Hpy188III TCNNGA 1 cut(s) 348
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 2 cut(s) 354, 554
HpyAV CCTTC 2 cut(s) 97, 276
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 3 cut(s) 460, 561, 601
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 3 cut(s) 315, 461, 593
Kzo9I GATC 1 cut(s) 290
LmnI GCTCC 5 cut(s) 106, 407, 425, 433, 658
Lsp1109I GCAGC 2 cut(s) 398, 438
LweI GCATC 1 cut(s) 330
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 292
MboI GATC 1 cut(s) 290
MboII GAAGA 8 cut(s) 58, 254, 257, 267, 270, 285, 454, 637
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 3 cut(s) 111, 192, 430
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 7 cut(s) 12, 130, 221, 229, 473, 636, 641
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 2 cut(s) 70, 382
MmeI TCCRAC 1 cut(s) 387
Mox20I TGGCCA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 536
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 114, 199
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 114, 199
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 3 cut(s) 460, 561, 601
NdeI CATATG 1 cut(s) 120
NdeII GATC 1 cut(s) 290
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 2 cut(s) 276, 619
NmeAIII GCCGAG 2 cut(s) 303, 480
NmuCI GTSAC 1 cut(s) 544
PaeR7I CTCGAG 1 cut(s) 329
PaqCI CACCTGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 388, 453
PleI GAGTC 2 cut(s) 70, 381
PpsI GAGTC 2 cut(s) 70, 381
Psp124BI GAGCTC 2 cut(s) 192, 430
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 112, 197
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 112, 197
PspN4I GGNNCC 2 cut(s) 276, 619
PspPI GGNCC 1 cut(s) 617
PspXI VCTCGAGB 1 cut(s) 329
PsuI RGATCY 1 cut(s) 290
SacI GAGCTC 2 cut(s) 192, 430
SatI GCNGC 2 cut(s) 387, 452
Sau3AI GATC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 2 cut(s) 70, 382
ScrFI CCNGG 2 cut(s) 114, 199
SduI GDGCHC 3 cut(s) 111, 192, 430
SfaNI GCATC 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 191, 329
SmoI CTYRAG 2 cut(s) 191, 329
Sse9I AATT 7 cut(s) 12, 130, 221, 229, 473, 636, 641
SsiI CCGC 1 cut(s) 179
SstI GAGCTC 2 cut(s) 192, 430
StyD4I CCNGG 2 cut(s) 112, 197
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 330, 349
TasI AATT 7 cut(s) 12, 130, 221, 229, 473, 636, 641
TfiI GAWTC 1 cut(s) 236
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 2 cut(s) 386, 451
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 59, 455
XapI RAATTY 2 cut(s) 130, 636
XhoI CTCGAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.