AT1G66680

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014371
Physical Location & Seq
Reverse (-)
24866095 .. 24869087
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66680.1

Sequence Viewer

Length: 1077 bp
ATGGCGGGAATCAGATTGCTACCGGAAGAACCTGAGACGACTCCTCAACAACAAGCCAGAGCCGCCGCCGCGGTGACAACGACAACAACGGATAGTTTGGCTTCCGATGACGATCGATCTATCGCGGCGGATTCGTGGTCGATCAAAAGTGAGTATGGAAGCACTCTCGATGACGATCAACGTCACGCCGATGCCGCCGAGGCTCTCTCGTCCGCTAACTTCCGTGTCTCTTCCGATTACAGTTCTGATAAGGAAGAGCCAGATGCTGATGGAGGAGGTCAATCAATGCTTGGTCTTCAAAGCTATTGGGATGCAGCGTATTCCGATGAGCTTACTAATTTCAGAGAGCATGGTCATGCTGGTGAAGTTTGGTTTGGAGATGATGTGATGGAGATTGTTACTTCTTGGACTAAAGACTTATGCGTTGAGATTTCTCAAAGAAACATGTCTGTTTCTGAAAACGATGTTACGACAGAGGTGAATGATCAGGCTGATAAGTATTTGTCTAGTTGGAATGTGCTTGATCTTGGTACTGGAAATGGCTTACTCCTTCATCAACTTGCTAAGGAAGGGTTCTCTGATTTAACCGGAACTGATTACAGTGACGGGGCTGTAGAACTCGCTCAACATCTTTCCCAACGTGATGGCTTTCCAAATATCCGTTTCATGGTTGATGATATCCTTGACACAAAATTGGAACAGCAGTTCAAGCTGGTAATGGACAAAGGAACTTTAGATGCTATTGGTTTGCATCCTGATGGCCCTGTCAAAAGAGTCATGTACTGGGATTCAGTGTCCAAGCTGGTTGCTCCTGGTGGAATATTGGTGATCACATCGTGTAACCACACAAAGGACGAACTGGTGGAAGAAGTAGAGAATTTCAACATCAGAAAATCCAACTTATGTAGAGGAGATGGAAATGACGCAAACAATGTACTTTCCTCTGGCTCTGAAGCTGCAAGCAGAATTGATCAACCTCCGTTTGAGTATCTCAGCCATGTTAGGACATACCCGACGTTCATGTTCAGTGGGTCTGTAGGATCGCGTGTGGCAACGGTTGCTTTTCTGCGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

358

Amino Acids

39.23

Weight (kDa)

4.42

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 163 - 281 2.3e-06 Methyltransferase domain
MTS PF05175 170 - 240 3.9e-06 Methyltransferase small domain
Methyltransf_31 PF13847 171 - 293 1e-19 Methyltransferase domain
Methyltransf_25 PF13649 173 - 246 2.3e-11 Methyltransferase domain
Methyltransf_12 PF08242 174 - 275 2.3e-08 Methyltransferase domain
Methyltransf_11 PF08241 174 - 277 2.1e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 71, 125, 1045
AclWI GGATC 1 cut(s) 1048
AcsI RAATTY 1 cut(s) 877
AcuI CTGAAG 1 cut(s) 972
AdeI CACNNNGTG 1 cut(s) 837
AfaI GTAC 3 cut(s) 532, 782, 936
AfiI CCNNNNNNNGG 2 cut(s) 667, 850
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 3 cut(s) 299, 709, 883
AjnI CCWGG 1 cut(s) 811
AjuI GAANNNNNNNTTGG 2 cut(s) 357, 389
AloI GAACNNNNNNTCC 2 cut(s) 689, 721
AluBI AGCT 5 cut(s) 303, 331, 712, 802, 956
AluI AGCT 5 cut(s) 303, 331, 712, 802, 956
Alw26I GTCTC 2 cut(s) 29, 232
AlwI GGATC 1 cut(s) 1048
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 760
ApeKI GCWGC 2 cut(s) 314, 956
ApoI RAATTY 1 cut(s) 877
AspS9I GGNCC 1 cut(s) 761
AsuHPI GGTGA 4 cut(s) 85, 374, 490, 838
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 326, 943
BccI CCATC 5 cut(s) 263, 382, 638, 752, 908
BciT130I CCWGG 1 cut(s) 813
BclI TGATCA 3 cut(s) 484, 828, 970
BcoDI GTCTC 2 cut(s) 29, 232
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 2 cut(s) 612, 1035
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 7 cut(s) 63, 66, 69, 126, 195, 315, 957
BlsI GCNGC 7 cut(s) 64, 67, 70, 127, 196, 316, 958
Bme1390I CCNGG 1 cut(s) 813
BmgT120I GGNCC 1 cut(s) 761
BmrFI CCNGG 1 cut(s) 813
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 5 cut(s) 181, 253, 301, 727, 760
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 191, 223
Bpu10I CCTNAGC 1 cut(s) 564
Bsa29I ATCGAT 1 cut(s) 115
BsaBI GATNNNNATC 2 cut(s) 111, 174
BsaJI CCNNGG 2 cut(s) 69, 198
BsaWI WCCGGW 2 cut(s) 22, 587
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 2 cut(s) 667, 850
Bse1I ACTGG 3 cut(s) 538, 788, 864
Bse8I GATNNNNATC 2 cut(s) 111, 174
BseBI CCWGG 1 cut(s) 813
BseCI ATCGAT 1 cut(s) 115
BseDI CCNNGG 2 cut(s) 69, 198
BseGI GGATG 2 cut(s) 316, 751
BseJI GATNNNNATC 2 cut(s) 111, 174
BseLI CCNNNNNNNGG 2 cut(s) 667, 850
BseMII CTCAG 2 cut(s) 24, 1006
BseNI ACTGG 3 cut(s) 538, 788, 864
BseRI GAGGAG 3 cut(s) 33, 288, 924
BseXI GCAGC 2 cut(s) 326, 943
Bsh1236I CGCG 3 cut(s) 71, 125, 1045
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 762
BshVI ATCGAT 1 cut(s) 115
BsiEI CGRYCG 1 cut(s) 115
BsiSI CCGG 2 cut(s) 23, 588
BslI CCNNNNNNNGG 2 cut(s) 667, 850
BsmAI GTCTC 2 cut(s) 29, 232
BsmBI CGTCTC 1 cut(s) 29
BsnI GGCC 1 cut(s) 762
Bsp143I GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
BspANI GGCC 1 cut(s) 762
BspCNI CTCAG 2 cut(s) 25, 1005
BspDI ATCGAT 1 cut(s) 115
BspFNI CGCG 3 cut(s) 71, 125, 1045
BspPI GGATC 1 cut(s) 1048
BspQI GCTCTTC 1 cut(s) 249
BsrI ACTGG 3 cut(s) 538, 788, 864
BssECI CCNNGG 2 cut(s) 69, 198
BssMI GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 3 cut(s) 242, 602, 1057
Bst6I CTCTTC 2 cut(s) 235, 249
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstC8I GCNNGC 1 cut(s) 961
BstDEI CTNAG 3 cut(s) 33, 564, 992
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 316, 751
BstFNI CGCG 3 cut(s) 71, 125, 1045
BstKTI GATC 9 cut(s) 115, 119, 144, 178, 487, 526, 831, 973, 1043
BstMAI GTCTC 2 cut(s) 29, 232
BstMBI GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 6 cut(s) 62, 68, 194, 200, 709, 1058
BstNI CCWGG 1 cut(s) 813
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 811
BstSFI CTRYAG 2 cut(s) 612, 1035
BstUI CGCG 3 cut(s) 71, 125, 1045
BstV1I GCAGC 2 cut(s) 326, 943
BstV2I GAAGAC 1 cut(s) 287
BstXI CCANNNNNNTGG 1 cut(s) 644
Bsu15I ATCGAT 1 cut(s) 115
BsuRI GGCC 1 cut(s) 762
BsuTUI ATCGAT 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 2 cut(s) 316, 751
BtsIMutI CAGTG 3 cut(s) 607, 798, 1033
Cac8I GCNNGC 1 cut(s) 961
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 761
Cfr42I CCGCGG 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 115
CseI GACGC 1 cut(s) 932
Csp6I GTAC 3 cut(s) 531, 781, 935
CviAII CATG 7 cut(s) 350, 356, 445, 667, 778, 998, 1021
CviQI GTAC 3 cut(s) 531, 781, 935
DdeI CTNAG 3 cut(s) 33, 564, 992
DpnI GATC 9 cut(s) 114, 118, 143, 177, 486, 525, 830, 972, 1042
DpnII GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
DraIII CACNNNGTG 1 cut(s) 837
Eam1104I CTCTTC 2 cut(s) 235, 249
EarI CTCTTC 2 cut(s) 235, 249
EciI GGCGGA 1 cut(s) 143
Eco32I GATATC 1 cut(s) 679
Eco57I CTGAAG 1 cut(s) 972
EcoRII CCWGG 1 cut(s) 811
EcoRV GATATC 1 cut(s) 679
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 7 cut(s) 353, 359, 448, 670, 781, 1001, 1024
FatI CATG 7 cut(s) 349, 355, 444, 666, 777, 997, 1020
FbaI TGATCA 3 cut(s) 484, 828, 970
Fnu4HI GCNGC 7 cut(s) 63, 66, 69, 126, 195, 315, 957
FokI GGATG 2 cut(s) 323, 738
Fsp4HI GCNGC 7 cut(s) 63, 66, 69, 126, 195, 315, 957
FspBI CTAG 1 cut(s) 507
GluI GCNGC 7 cut(s) 63, 66, 69, 126, 195, 315, 957
HaeIII GGCC 1 cut(s) 762
HapII CCGG 2 cut(s) 23, 588
HgaI GACGC 1 cut(s) 932
Hin1II CATG 7 cut(s) 353, 359, 448, 670, 781, 1001, 1024
HinfI GANTC 5 cut(s) 9, 40, 131, 774, 788
HpaII CCGG 2 cut(s) 23, 588
HphI GGTGA 4 cut(s) 85, 374, 490, 838
Hpy188III TCNNGA 2 cut(s) 167, 755
Hpy99I CGWCG 1 cut(s) 1018
HpyAV CCTTC 2 cut(s) 560, 563
HpyCH4III ACNGT 3 cut(s) 242, 602, 1057
HpyCH4IV ACGT 3 cut(s) 181, 640, 1016
HpyCH4V TGCA 3 cut(s) 314, 751, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 68, 194, 200, 709, 1058
HpyF3I CTNAG 3 cut(s) 33, 564, 992
HpySE526I ACGT 3 cut(s) 181, 640, 1016
Hsp92II CATG 7 cut(s) 353, 359, 448, 670, 781, 1001, 1024
Ksp22I TGATCA 3 cut(s) 484, 828, 970
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 2 cut(s) 326, 943
LweI GCATC 5 cut(s) 181, 253, 301, 727, 760
MaeI CTAG 1 cut(s) 507
MaeII ACGT 3 cut(s) 181, 640, 1016
MaeIII GTNAC 6 cut(s) 73, 182, 397, 466, 602, 839
MalI GATC 9 cut(s) 114, 118, 143, 177, 486, 525, 830, 972, 1042
MboI GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
MboII GAAGA 5 cut(s) 38, 222, 266, 287, 878
MluCI AATT 4 cut(s) 337, 692, 877, 966
MlyI GAGTC 2 cut(s) 34, 783
MmeI TCCRAC 2 cut(s) 491, 921
MnlI CCTC 8 cut(s) 54, 193, 266, 269, 469, 902, 952, 987
MseI TTAA 1 cut(s) 584
MslI CAYNNNNRTG 4 cut(s) 189, 354, 360, 756
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 23, 588
MspR9I CCNGG 1 cut(s) 813
MvaI CCWGG 1 cut(s) 813
MvnI CGCG 3 cut(s) 71, 125, 1045
MwoI GCNNNNNNNGC 6 cut(s) 62, 68, 194, 200, 709, 1058
NdeII GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
NlaIII CATG 7 cut(s) 353, 359, 448, 670, 781, 1001, 1024
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 3 cut(s) 73, 182, 602
NspI RCATGY 1 cut(s) 448
PciI ACATGT 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 249
PfeI GAWTC 3 cut(s) 9, 131, 788
PkrI GCNGC 7 cut(s) 64, 67, 70, 127, 196, 316, 958
Ple19I CGATCG 1 cut(s) 115
PleI GAGTC 2 cut(s) 34, 782
PpsI GAGTC 2 cut(s) 34, 782
PscI ACATGT 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 811
PspGI CCWGG 1 cut(s) 811
PspPI GGNCC 1 cut(s) 761
PstNI CAGNNNCTG 1 cut(s) 266
PvuI CGATCG 1 cut(s) 115
RsaI GTAC 3 cut(s) 532, 782, 936
RsaNI GTAC 3 cut(s) 531, 781, 935
RseI CAYNNNNRTG 4 cut(s) 189, 354, 360, 756
SacII CCGCGG 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 7 cut(s) 63, 66, 69, 126, 195, 315, 957
Sau3AI GATC 9 cut(s) 112, 116, 141, 175, 484, 523, 828, 970, 1040
Sau96I GGNCC 1 cut(s) 761
SchI GAGTC 2 cut(s) 34, 783
ScrFI CCNGG 1 cut(s) 813
SfaNI GCATC 5 cut(s) 181, 253, 301, 727, 760
SfcI CTRYAG 2 cut(s) 612, 1035
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SmiMI CAYNNNNRTG 4 cut(s) 189, 354, 360, 756
Sse9I AATT 4 cut(s) 337, 692, 877, 966
SspI AATATT 1 cut(s) 822
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 811
TaaI ACNGT 3 cut(s) 242, 602, 1057
TaiI ACGT 3 cut(s) 184, 643, 1019
TaqI TCGA 3 cut(s) 115, 140, 168
TasI AATT 4 cut(s) 337, 692, 877, 966
TatI WGTACW 2 cut(s) 780, 934
TauI GCSGC 5 cut(s) 65, 68, 71, 128, 197
TfiI GAWTC 3 cut(s) 9, 131, 788
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 3 cut(s) 607, 798, 1033
TseFI GTSAC 3 cut(s) 73, 182, 602
TseI GCWGC 2 cut(s) 314, 956
Tsp45I GTSAC 3 cut(s) 73, 182, 602
TspDTI ATGAA 3 cut(s) 542, 655, 1009
TspGWI ACGGA 4 cut(s) 104, 212, 650, 969
TspRI CASTG 3 cut(s) 607, 798, 1033
XapI RAATTY 1 cut(s) 877
XceI RCATGY 1 cut(s) 448
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.