Rroxscaffold_6G00391390

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014371
Physical Location & Seq
Forward (+)
9382133 .. 9386470
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391390.1

Sequence Viewer

Length: 1008 bp
ATGGCCGGAATCCGATTGGGCCCTGATGACTCAGAGATATCGCAGCAGAGCCGAGCTGCTCCGGCCGATCTTATCTCAGACGACGATCGCTCCGTCGCCGCCGACTCGTGGTCCATCAAGAGCGACTATGGGAGCACTCTCGACGACGACCAACGCCACGCTGATGCTGCTGAGGATCTCTCCGCCGCTAACCTCCGTGCCCCCTCCGATTACAACTCTGACAAGGAGGAACCGGATTCTGATGGGATATCATCAATGCTAGGCCTTCAGAGTTATTGGGATGCTGCCTATGCAGATGAGCTGGTTAATTTTCGTGAACATGGGCATGCTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTTGCCTCTTGGACCAAAACATTATGCATTGATGCTTCTCAAGGTCGCATGCCAAATCATGTTGATAATAGCAAGTCTGAATCAGCTGAACAAGGTTACAAGTTTCTTTCAGGTTGGAGTGTACTTGACATTGGGACTGGCAATGGATTGCTTCTTCAAGAACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAATTGATTATAGTGAAGGGGCGATTGACCTTGCTCGAAACCTCGCTGACCGTGATGGATTACCCAATATTCATTTTTTGGTTGATGATATTCTAGAGACGAAGTTGGAACGACAGTTTCAGCTTGTTGTAGACAAAGGAACCTTAGATGCCATTGGATTGCATCCAGATGGTGCTATAAAAAGGATAATATATTGGGATTCAGTTTCGAGATTGATGGCTCCTGGTGGAATATTAGTAATCACATCATGTAACAGCACTAAAGAAGAATTAGTGCAAGAAGTGGAAAATTTCAATCAAAGAAGGATTGGTGTGACCCAAGATCAAGAAGGATGCAAAGATACGGCTCCTCAGCTTCAATACCTTAGTCATGTTCGCACATATCCAACGTTCATGTTTGGTGGATCTGTAGGATCACGGGTTGCCACTGTGGCATTTCTCCGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

335

Amino Acids

36.66

Weight (kDa)

4.44

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 159 - 285 2e-20 Methyltransferase domain
Methyltransf_23 PF13489 159 - 273 3.4e-10 Methyltransferase domain
MTS PF05175 162 - 268 7.4e-06 Methyltransferase small domain
Methyltransf_25 PF13649 163 - 238 7.5e-13 Methyltransferase domain
Methyltransf_12 PF08242 164 - 265 5.6e-10 Methyltransferase domain
Methyltransf_11 PF08241 164 - 267 5.6e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 690
AccIII TCCGGA 1 cut(s) 999
AciI CCGC 3 cut(s) 99, 183, 186
AclI AACGTT 1 cut(s) 947
AclWI GGATC 3 cut(s) 183, 970, 979
AcoI YGGCCR 2 cut(s) 3, 63
AcsI RAATTY 1 cut(s) 847
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 2 cut(s) 108, 540
AgsI TTSAA 3 cut(s) 524, 853, 917
AhdI GACNNNNNGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 781
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 6 cut(s) 56, 301, 350, 452, 682, 913
AluI AGCT 6 cut(s) 56, 301, 350, 452, 682, 913
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 3 cut(s) 183, 970, 979
Aor13HI TCCGGA 1 cut(s) 999
AoxI GGCC 4 cut(s) 3, 19, 63, 262
ApaI GGGCCC 1 cut(s) 23
ApeKI GCWGC 4 cut(s) 43, 56, 167, 284
ApoI RAATTY 1 cut(s) 847
AspS9I GGNCC 4 cut(s) 19, 20, 111, 378
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 2 cut(s) 111, 378
BaeGI GKGCMC 2 cut(s) 23, 202
BanII GRGCYC 1 cut(s) 23
BauI CACGAG 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 2 cut(s) 171, 909
BbvI GCAGC 4 cut(s) 43, 55, 154, 271
BccI CCATC 5 cut(s) 122, 236, 608, 722, 769
BceAI ACGGC 1 cut(s) 918
BciT130I CCWGG 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 2 cut(s) 260, 653
BfmI CTRYAG 1 cut(s) 966
BglI GCCNNNNNGGC 1 cut(s) 989
BisI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
BlsI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 2 cut(s) 111, 378
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 4 cut(s) 19, 20, 111, 378
BmiI GGNNCC 5 cut(s) 21, 231, 700, 780, 906
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 6 cut(s) 154, 271, 388, 697, 730, 881
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu10I CCTNAGC 2 cut(s) 171, 909
BpuEI CTTGAG 1 cut(s) 390
BsaWI WCCGGW 2 cut(s) 232, 999
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 339, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 540
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 514
BseAI TCCGGA 1 cut(s) 999
BseBI CCWGG 1 cut(s) 783
BseGI GGATG 3 cut(s) 286, 721, 896
BseLI CCNNNNNNNGG 2 cut(s) 108, 540
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 4 cut(s) 45, 90, 162, 923
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 897
BseSI GKGCMC 2 cut(s) 23, 202
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 43, 55, 154, 271
Bsh1285I CGRYCG 2 cut(s) 66, 88
BshFI GGCC 4 cut(s) 5, 21, 65, 264
BsiEI CGRYCG 2 cut(s) 66, 88
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 4 cut(s) 6, 62, 233, 1000
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 108, 540
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 4 cut(s) 5, 21, 65, 264
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 3 cut(s) 23, 137, 202
Bsp13I TCCGGA 1 cut(s) 999
Bsp143I GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BspACI CCGC 3 cut(s) 99, 183, 186
BspANI GGCC 4 cut(s) 5, 21, 65, 264
BspCNI CTCAG 4 cut(s) 44, 89, 163, 922
BspEI TCCGGA 1 cut(s) 999
BspLI GGNNCC 5 cut(s) 21, 231, 700, 780, 906
BspPI GGATC 3 cut(s) 183, 970, 979
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 1 cut(s) 508
BssMI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 3 cut(s) 611, 675, 988
BstC8I GCNNGC 2 cut(s) 327, 416
BstDEI CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
BstF5I GGATG 3 cut(s) 286, 721, 896
BstKTI GATC 6 cut(s) 70, 88, 178, 883, 965, 974
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BstMCI CGRYCG 2 cut(s) 66, 88
BstMWI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
BstNI CCWGG 1 cut(s) 783
BstNSI RCATGY 2 cut(s) 329, 418
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 1 cut(s) 966
BstSLI GKGCMC 2 cut(s) 23, 202
BstV1I GCAGC 4 cut(s) 43, 55, 154, 271
BstX2I RGATCY 2 cut(s) 175, 962
BstYI RGATCY 2 cut(s) 175, 962
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 4 cut(s) 5, 21, 65, 264
BtsCI GGATG 3 cut(s) 286, 721, 896
BtsIMutI CAGTG 1 cut(s) 984
Cac8I GCNNGC 2 cut(s) 327, 416
Cfr13I GGNCC 4 cut(s) 19, 20, 111, 378
Csp6I GTAC 1 cut(s) 488
CviAII CATG 8 cut(s) 320, 326, 358, 415, 425, 807, 929, 952
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
DpnI GATC 6 cut(s) 69, 87, 177, 882, 964, 973
DpnII GATC 6 cut(s) 67, 85, 175, 880, 962, 971
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 3, 63
EagI CGGCCG 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 2 cut(s) 39, 249
Eco47I GGWCC 2 cut(s) 111, 378
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 781
EcoRV GATATC 2 cut(s) 39, 249
EcoT22I ATGCAT 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
FaqI GGGAC 1 cut(s) 514
FatI CATG 8 cut(s) 319, 325, 357, 414, 424, 806, 928, 951
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FokI GGATG 3 cut(s) 293, 708, 903
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FspBI CTAG 2 cut(s) 260, 653
GluI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
HaeIII GGCC 4 cut(s) 5, 21, 65, 264
HapII CCGG 4 cut(s) 6, 62, 233, 1000
Hin1II CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
HinfI GANTC 6 cut(s) 9, 29, 104, 236, 446, 758
HpaII CCGG 4 cut(s) 6, 62, 233, 1000
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 317, 488, 691
Hpy188I TCNGA 9 cut(s) 14, 34, 79, 208, 220, 241, 270, 445, 550
Hpy188III TCNNGA 9 cut(s) 118, 140, 314, 524, 653, 725, 768, 884, 1000
Hpy8I GTNNAC 3 cut(s) 317, 488, 691
Hpy99I CGWCG 4 cut(s) 86, 98, 146, 149
HpyAV CCTTC 4 cut(s) 275, 569, 855, 881
HpyCH4III ACNGT 3 cut(s) 611, 675, 988
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 5 cut(s) 293, 393, 721, 835, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
HpyF3I CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
Kpn2I TCCGGA 1 cut(s) 999
Kzo9I GATC 6 cut(s) 67, 85, 175, 880, 962, 971
LmnI GCTCC 6 cut(s) 64, 95, 132, 347, 784, 910
Lsp1109I GCAGC 4 cut(s) 43, 55, 154, 271
LweI GCATC 6 cut(s) 154, 271, 388, 697, 730, 881
MaeI CTAG 2 cut(s) 260, 653
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 3 cut(s) 461, 809, 871
MalI GATC 6 cut(s) 69, 87, 177, 882, 964, 973
MboI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
MboII GAAGA 2 cut(s) 512, 836
MflI RGATCY 2 cut(s) 175, 962
MhlI GDGCHC 3 cut(s) 23, 137, 202
MluCI AATT 5 cut(s) 307, 561, 827, 847, 1003
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 3 cut(s) 461, 645, 968
MnlI CCTC 7 cut(s) 166, 203, 214, 220, 382, 611, 918
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 1 cut(s) 999
MseI TTAA 2 cut(s) 306, 554
MslI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 4 cut(s) 6, 62, 233, 1000
MspR9I CCNGG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 783
MwoI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
NdeII GATC 6 cut(s) 67, 85, 175, 880, 962, 971
NlaIII CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
NlaIV GGNNCC 5 cut(s) 21, 231, 700, 780, 906
NmeAIII GCCGAG 1 cut(s) 77
NmuCI GTSAC 1 cut(s) 871
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 2 cut(s) 329, 418
PaeI GCATGC 2 cut(s) 329, 418
PceI AGGCCT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 4 cut(s) 9, 236, 446, 758
PkrI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
Psp1406I AACGTT 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 5 cut(s) 21, 231, 700, 780, 906
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 4 cut(s) 19, 20, 111, 378
PsuI RGATCY 2 cut(s) 175, 962
PvuI CGATCG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SaqAI TTAA 2 cut(s) 306, 554
SatI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
Sau3AI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
Sau96I GGNCC 4 cut(s) 19, 20, 111, 378
SchI GAGTC 2 cut(s) 23, 98
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 3 cut(s) 23, 137, 202
SfaNI GCATC 6 cut(s) 154, 271, 388, 697, 730, 881
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 2 cut(s) 111, 378
SmiMI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 2 cut(s) 329, 418
Sse9I AATT 5 cut(s) 307, 561, 827, 847, 1003
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 3 cut(s) 99, 183, 186
SspI AATATT 2 cut(s) 628, 792
SspMI CTAG 2 cut(s) 260, 653
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 781
TaaI ACNGT 3 cut(s) 611, 675, 988
TaiI ACGT 1 cut(s) 950
TaqI TCGA 3 cut(s) 141, 595, 767
TasI AATT 5 cut(s) 307, 561, 827, 847, 1003
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 101, 188
TfiI GAWTC 4 cut(s) 9, 236, 446, 758
Tru1I TTAA 2 cut(s) 306, 554
Tru9I TTAA 2 cut(s) 306, 554
TscAI CASTG 1 cut(s) 991
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 4 cut(s) 43, 56, 167, 284
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 2 cut(s) 620, 940
TspGWI ACGGA 2 cut(s) 82, 185
TspRI CASTG 1 cut(s) 991
VpaK11BI GGWCC 2 cut(s) 111, 378
XapI RAATTY 1 cut(s) 847
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 2 cut(s) 329, 418
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 2 cut(s) 260, 653
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.