FvH4_1g29040

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014371
Physical Location & Seq
Reverse (-)
21466386 .. 21470126
3741 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29040.t1

Sequence Viewer

Length: 867 bp
ATGGCCGGATTTCGATTGGGCCCTGATGACTCTGAGATATCGCAGCAGCAGAGCCGAGCTGCTCCGGCCGATCTCATCTCAGACGATGATCGCTCCGTCGCCGCGGACTCGTGGTCCATCAAGAGCGACTATGGCAGCACTCTTGACGACGACCTACGCCACGCTGATGCTGCTGAGGATCTCTCCGCCGCTAACCTCCGCGCCCCCTCCGATTACAGCTCTGACAAGGAGGAACCTGATTCGGACGGGGTGACATCAATGCTAGGTCTTCAGAGTTATTGGGATGCTGCTTATGCAGATGAGCTCGTTAATTTTCGTGAACATGGCCATGCTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTTGCTGCTTGGACCAAAACATTATGCATTGATGTTTCTCAAGGTCGCATGCCAAATCATGTTGATAATAACAAGTTTGAATATGTTGAGCAAGGTCACAAGTTCCTTTCAGGTTGGAGTGTACTTGACATTGGGACTGGCAACGGATTGCTGCTTCAAGAACTTTCCAAGCTGGGGTTTTCTGATTTAACAGGAACTGATTATAGTGAAGGGGCAATTGACCTTGCTCGAAACCTTGCTAACCGTGATGGATTACCGAATATTAATTTCTTGGTTGATGATATTCTAGAGACAAAGTTGGAACGCCAATTTCAACTTGTCGTAGACAAAGGAACCTTAGATGCCATCGGATTGCATCCAGACGGTGCCATCAAAAGGTTAGTATATTGGGATTCAGTTTCAAGATTGATGGCTCCTGGTGGAATATTGGTAAGCCTCTTCACTTGGCTGGTCCTTGTTACTCCAAATTTCACATACCTTCGTATAGGGATGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

289

Amino Acids

31.6

Weight (kDa)

4.34

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 160 - 268 6.9e-15 Methyltransferase domain
Methyltransf_23 PF13489 160 - 267 2.1e-07 Methyltransferase domain
Methyltransf_25 PF13649 164 - 239 3.3e-12 Methyltransferase domain
Methyltransf_12 PF08242 165 - 266 1.1e-09 Methyltransferase domain
Methyltransf_11 PF08241 165 - 267 1e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AccI GTMKAC 1 cut(s) 693
AccII CGCG 2 cut(s) 104, 201
AciI CCGC 5 cut(s) 102, 104, 186, 189, 199
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 3 cut(s) 3, 66, 325
AcsI RAATTY 1 cut(s) 835
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 543, 744
AgsI TTSAA 4 cut(s) 449, 527, 683, 771
AhdI GACNNNNNGTC 1 cut(s) 112
AjnI CCWGG 1 cut(s) 784
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 5 cut(s) 59, 219, 304, 353, 541
AluI AGCT 5 cut(s) 59, 219, 304, 353, 541
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 1 cut(s) 186
AlwNI CAGNNNCTG 1 cut(s) 566
AoxI GGCC 4 cut(s) 3, 19, 66, 325
ApaI GGGCCC 1 cut(s) 23
ApeKI GCWGC 8 cut(s) 43, 46, 59, 135, 170, 287, 374, 520
ApoI RAATTY 1 cut(s) 835
AseI ATTAAT 1 cut(s) 633
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 5 cut(s) 19, 20, 114, 381, 820
AsuHPI GGTGA 2 cut(s) 262, 347
AvaII GGWCC 3 cut(s) 114, 381, 820
BaeGI GKGCMC 1 cut(s) 23
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 2 cut(s) 23, 306
BauI CACGAG 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 8 cut(s) 46, 55, 58, 147, 157, 274, 361, 507
BccI CCATC 5 cut(s) 125, 611, 722, 746, 772
BciT130I CCWGG 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 2 cut(s) 263, 656
Bme1390I CCNGG 1 cut(s) 786
Bme18I GGWCC 3 cut(s) 114, 381, 820
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgT120I GGNCC 5 cut(s) 19, 20, 114, 381, 820
BmiI GGNNCC 5 cut(s) 21, 234, 703, 736, 783
BmrFI CCNGG 1 cut(s) 786
BmsI GCATC 4 cut(s) 157, 274, 700, 733
BpiI GAAGAC 1 cut(s) 260
BplI GAGNNNNNCTC 2 cut(s) 167, 199
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 543, 744
Bse1I ACTGG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 3 cut(s) 289, 724, 864
BseLI CCNNNNNNNGG 2 cut(s) 543, 744
BseMII CTCAG 3 cut(s) 24, 93, 165
BseNI ACTGG 1 cut(s) 511
BseSI GKGCMC 1 cut(s) 23
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 8 cut(s) 46, 55, 58, 147, 157, 274, 361, 507
BseYI CCCAGC 1 cut(s) 541
Bsh1236I CGCG 2 cut(s) 104, 201
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 5, 21, 68, 327
BshNI GGYRCC 1 cut(s) 734
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 6, 65
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 543, 744
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 4 cut(s) 5, 21, 68, 327
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 23, 306
Bsp143I GATC 3 cut(s) 70, 88, 178
BspACI CCGC 5 cut(s) 102, 104, 186, 189, 199
BspANI GGCC 4 cut(s) 5, 21, 68, 327
BspCNI CTCAG 3 cut(s) 25, 92, 166
BspFNI CGCG 2 cut(s) 104, 201
BspLI GGNNCC 5 cut(s) 21, 234, 703, 736, 783
BspPI GGATC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 734
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 70, 88, 178
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 2 cut(s) 614, 734
Bst6I CTCTTC 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 4 cut(s) 33, 79, 174, 706
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 289, 724, 864
BstFNI CGCG 2 cut(s) 104, 201
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 3 cut(s) 73, 91, 181
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 3 cut(s) 70, 88, 178
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 4 cut(s) 65, 132, 170, 293
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 784
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 2 cut(s) 104, 201
BstV1I GCAGC 8 cut(s) 46, 55, 58, 147, 157, 274, 361, 507
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BstZI CGGCCG 1 cut(s) 66
BsuRI GGCC 4 cut(s) 5, 21, 68, 327
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 289, 724, 864
Cac8I GCNNGC 1 cut(s) 419
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 5 cut(s) 19, 20, 114, 381, 820
Cfr42I CCGCGG 1 cut(s) 105
Csp6I GTAC 1 cut(s) 491
CviAII CATG 5 cut(s) 323, 329, 361, 418, 428
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 4 cut(s) 33, 79, 174, 706
DpnI GATC 3 cut(s) 72, 90, 180
DpnII GATC 3 cut(s) 70, 88, 178
DriI GACNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 3 cut(s) 3, 66, 325
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 812
Eam1105I GACNNNNNGTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 812
EciI GGCGGA 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 23, 306
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 114, 381, 820
Eco52I CGGCCG 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 784
EcoRV GATATC 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 398
EcoT38I GRGCYC 2 cut(s) 23, 306
FaeI CATG 5 cut(s) 326, 332, 364, 421, 431
FaqI GGGAC 1 cut(s) 517
FatI CATG 5 cut(s) 322, 328, 360, 417, 427
FblI GTMKAC 1 cut(s) 693
FokI GGATG 2 cut(s) 296, 711
FriOI GRGCYC 2 cut(s) 23, 306
FspBI CTAG 2 cut(s) 263, 656
GlaI GCGC 1 cut(s) 202
GsaI CCCAGC 1 cut(s) 545
HaeIII GGCC 4 cut(s) 5, 21, 68, 327
HapII CCGG 2 cut(s) 6, 65
HhaI GCGC 1 cut(s) 203
Hin1II CATG 5 cut(s) 326, 332, 364, 421, 431
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 4 cut(s) 29, 107, 239, 761
HpaII CCGG 2 cut(s) 6, 65
HphI GGTGA 2 cut(s) 262, 347
Hpy166II GTNNAC 3 cut(s) 320, 491, 694
Hpy188I TCNGA 8 cut(s) 34, 82, 211, 223, 244, 273, 553, 719
Hpy188III TCNNGA 7 cut(s) 121, 143, 317, 527, 656, 728, 771
Hpy8I GTNNAC 3 cut(s) 320, 491, 694
Hpy99I CGWCG 2 cut(s) 101, 152
HpyAV CCTTC 2 cut(s) 572, 857
HpyCH4III ACNGT 2 cut(s) 614, 734
HpyCH4V TGCA 3 cut(s) 296, 396, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 132, 170, 293
HpyF3I CTNAG 4 cut(s) 33, 79, 174, 706
Hsp92II CATG 5 cut(s) 326, 332, 364, 421, 431
HspAI GCGC 1 cut(s) 201
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 3 cut(s) 70, 88, 178
LmnI GCTCC 4 cut(s) 67, 98, 350, 787
Lsp1109I GCAGC 8 cut(s) 46, 55, 58, 147, 157, 274, 361, 507
LweI GCATC 4 cut(s) 157, 274, 700, 733
MaeI CTAG 2 cut(s) 263, 656
MaeIII GTNAC 3 cut(s) 250, 464, 826
MalI GATC 3 cut(s) 72, 90, 180
MboI GATC 3 cut(s) 70, 88, 178
MboII GAAGA 2 cut(s) 260, 799
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 2 cut(s) 23, 306
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 5 cut(s) 310, 585, 634, 677, 835
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 2 cut(s) 23, 101
MmeI TCCRAC 2 cut(s) 464, 648
MnlI CCTC 5 cut(s) 169, 206, 217, 223, 815
Mox20I TGGCCA 1 cut(s) 327
Mph1103I ATGCAT 1 cut(s) 398
MscI TGGCCA 1 cut(s) 327
MseI TTAA 3 cut(s) 309, 557, 633
MslI CAYNNNNRTG 3 cut(s) 165, 327, 333
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 6, 65
MspR9I CCNGG 1 cut(s) 786
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 2 cut(s) 104, 201
MwoI GCNNNNNNNGC 4 cut(s) 65, 132, 170, 293
NdeII GATC 3 cut(s) 70, 88, 178
NlaIII CATG 5 cut(s) 326, 332, 364, 421, 431
NlaIV GGNNCC 5 cut(s) 21, 234, 703, 736, 783
NmeAIII GCCGAG 1 cut(s) 80
NmuCI GTSAC 2 cut(s) 250, 464
NsiI ATGCAT 1 cut(s) 398
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PfeI GAWTC 2 cut(s) 239, 761
PleI GAGTC 2 cut(s) 23, 101
PpsI GAGTC 2 cut(s) 23, 101
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 5 cut(s) 21, 234, 703, 736, 783
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 5 cut(s) 19, 20, 114, 381, 820
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 178
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 3 cut(s) 165, 327, 333
SacI GAGCTC 1 cut(s) 306
SacII CCGCGG 1 cut(s) 105
SaqAI TTAA 3 cut(s) 309, 557, 633
Sau3AI GATC 3 cut(s) 70, 88, 178
Sau96I GGNCC 5 cut(s) 19, 20, 114, 381, 820
SchI GAGTC 2 cut(s) 23, 101
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 2 cut(s) 23, 306
SfaNI GCATC 4 cut(s) 157, 274, 700, 733
Sfr303I CCGCGG 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 105
SinI GGWCC 3 cut(s) 114, 381, 820
SmiMI CAYNNNNRTG 3 cut(s) 165, 327, 333
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
SphI GCATGC 1 cut(s) 421
Sse9I AATT 5 cut(s) 310, 585, 634, 677, 835
SsiI CCGC 5 cut(s) 102, 104, 186, 189, 199
SspI AATATT 2 cut(s) 631, 795
SspMI CTAG 2 cut(s) 263, 656
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 784
TaaI ACNGT 2 cut(s) 614, 734
TaqI TCGA 2 cut(s) 13, 598
TasI AATT 5 cut(s) 310, 585, 634, 677, 835
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 104, 191
TfiI GAWTC 2 cut(s) 239, 761
Tru1I TTAA 3 cut(s) 309, 557, 633
Tru9I TTAA 3 cut(s) 309, 557, 633
TseFI GTSAC 2 cut(s) 250, 464
TseI GCWGC 8 cut(s) 43, 46, 59, 135, 170, 287, 374, 520
Tsp45I GTSAC 2 cut(s) 250, 464
TspGWI ACGGA 2 cut(s) 85, 528
VpaK11BI GGWCC 3 cut(s) 114, 381, 820
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 1 cut(s) 835
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 2 cut(s) 263, 656
Zsp2I ATGCAT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.