MD17G1253400.v1.1

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014371
Physical Location & Seq
Forward (+)
30699408 .. 30710104
10697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1253400.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCAGGATTTCGATTGGGGCAAGATGACTCGGAGATATCGCAGCAGAGCCGAGCAGCACCGCCCGATCTCGTCTCTGACGATGAACGCTCCGTCGCCGCCGATTCATGGTCCATCAAGAGTGATTATGGAAGCACTCTCGACGACGACCAGCGCCATGCCGACGCCGCCGAGGACCTCTCTGCTGCTAACCTCCGTGCCCCCTCCGATTACAGTTCTGACAAGGAGGAACCAGATGCTGAAGGGGTGACATCAATGCTAGGTCTTCAGAGTTATTGGGATGCTGCATATGCAGATGAGTTGGCAAATTTTCGGGAACATGGTCATATCGGTGATGTTTGGTTTGGAGCTGATGTCATGGAAGTGGTCGCCTCTTGGACCAAAAGCTTATGCATTGATATTTCTAAAGGTCGCACACCAAATCATGTTGATAATAATAAGTCTGATCCTGTGGAACAAGATGATAAGTATCTTTCAGGCTGGAGTGTACTTGACATGGGGACTGGCAATGGATTGCTTCTTCAAGAACTTTCCAAGCAGGGGTTCTCTGATTTAACAGGAACTGATTATAGTGAAGGGGCGATTGACCTTGCTCGAAGCCGTGCTGATCGTGAAGGATTGCCAAACATTAAATTTTTGGTTGATGATATTCTAGAGACCAAGTTGGAAAGGCAGTTCCAACTAGTTGTGGACAAAGGAACCTTGGATGCCATTGGATTGCATCCAGATGGTTCTATCAAAAGGCTAATTTATTGGGATTCAGTTTCACGGTTGGTGGCTTCTGGTGGATTAGTAGTGATCACATCATGTAACAACACAAAGGATGAATTGGTGCAAGAAGTGGAAAGTTTCAATCAAAGAAGTGCGACGCAGGAACATGAAACACTACCTAATCTTGGTCATGTCCGCACATATCCAACATTCATGTTTCGTGGATCCGTAGGGTCACGTGTTGCCACTGTGGCTTTTCTCCGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

327

Amino Acids

35.81

Weight (kDa)

4.49

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 159 - 286 1.2e-17 Methyltransferase domain
Methyltransf_23 PF13489 162 - 287 3e-08 Methyltransferase domain
MTS PF05175 162 - 281 1.3e-06 Methyltransferase small domain
Methyltransf_25 PF13649 163 - 236 1.3e-12 Methyltransferase domain
Methyltransf_11 PF08241 164 - 266 7e-09 Methyltransferase domain
Methyltransf_12 PF08242 164 - 264 6.1e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 972
AciI CCGC 4 cut(s) 62, 99, 168, 907
AclWI GGATC 3 cut(s) 440, 930, 943
AcsI RAATTY 2 cut(s) 307, 632
AcuI CTGAAG 2 cut(s) 251, 261
AcvI CACGTG 1 cut(s) 950
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 3 cut(s) 108, 540, 896
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 2 cut(s) 524, 853
AhlI ACTAGT 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 2 cut(s) 350, 387
AluI AGCT 2 cut(s) 350, 387
Alw26I GTCTC 2 cut(s) 79, 650
AlwI GGATC 3 cut(s) 440, 930, 943
AlwNI CAGNNNCTG 2 cut(s) 239, 563
Aor13HI TCCGGA 1 cut(s) 972
ApeKI GCWGC 4 cut(s) 43, 56, 185, 284
ApoI RAATTY 2 cut(s) 307, 632
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 3 cut(s) 111, 175, 378
AsuHPI GGTGA 2 cut(s) 259, 344
AvaII GGWCC 3 cut(s) 111, 175, 378
BaeGI GKGCMC 1 cut(s) 202
BamHI GGATCC 1 cut(s) 935
BbrPI CACGTG 1 cut(s) 950
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 55, 68, 172, 271
BccI CCATC 2 cut(s) 122, 722
BceAI ACGGC 1 cut(s) 585
BclI TGATCA 1 cut(s) 798
BcoDI GTCTC 2 cut(s) 79, 650
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 4 cut(s) 260, 653, 683, 979
BfoI RGCGCY 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 962
BisI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
BlsI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Bme18I GGWCC 3 cut(s) 111, 175, 378
BmgT120I GGNCC 3 cut(s) 111, 175, 378
BmiI GGNNCC 3 cut(s) 231, 700, 937
BmsI GCATC 4 cut(s) 226, 271, 697, 730
BpiI GAAGAC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 502
BsaAI YACGTR 1 cut(s) 950
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 171, 702
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 540, 896
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 171, 702
BseGI GGATG 4 cut(s) 286, 712, 721, 829
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 108, 540, 896
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 202
BseXI GCAGC 4 cut(s) 55, 68, 172, 271
BsiSI CCGG 1 cut(s) 973
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 3 cut(s) 108, 540, 896
BsmAI GTCTC 2 cut(s) 79, 650
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 514
Bso31I GGTCTC 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 202
Bsp13I TCCGGA 1 cut(s) 972
Bsp143I GATC 5 cut(s) 67, 445, 607, 798, 935
BspACI CCGC 4 cut(s) 62, 99, 168, 907
BspEI TCCGGA 1 cut(s) 972
BspLI GGNNCC 3 cut(s) 231, 700, 937
BspPI GGATC 3 cut(s) 440, 930, 943
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 2 cut(s) 171, 702
BssMI GATC 5 cut(s) 67, 445, 607, 798, 935
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 702
Bst4CI ACNGT 3 cut(s) 215, 771, 961
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 950
BstF5I GGATG 4 cut(s) 286, 712, 721, 829
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 5 cut(s) 70, 448, 610, 801, 938
BstMAI GTCTC 2 cut(s) 79, 650
BstMBI GATC 5 cut(s) 67, 445, 607, 798, 935
BstMWI GCNNNNNNNGC 3 cut(s) 167, 290, 962
BstSLI GKGCMC 1 cut(s) 202
BstV1I GCAGC 4 cut(s) 55, 68, 172, 271
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 935
BstYI RGATCY 1 cut(s) 935
BtsCI GGATG 4 cut(s) 286, 712, 721, 829
BtsIMutI CAGTG 1 cut(s) 957
CaiI CAGNNNCTG 2 cut(s) 239, 563
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 111, 175, 378
CseI GACGC 2 cut(s) 173, 877
Csp6I GTAC 1 cut(s) 488
CviJI RGCY 8 cut(s) 51, 350, 387, 480, 600, 745, 779, 965
CviKI_1 RGCY 8 cut(s) 51, 350, 387, 480, 600, 745, 779, 965
CviQI GTAC 1 cut(s) 488
DpnI GATC 5 cut(s) 69, 447, 609, 800, 937
DpnII GATC 5 cut(s) 67, 445, 607, 798, 935
Eco130I CCWWGG 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 111, 175, 378
Eco57I CTGAAG 2 cut(s) 251, 261
Eco72I CACGTG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 175
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 79
FaqI GGGAC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FokI GGATG 4 cut(s) 293, 708, 719, 836
Fsp4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FspBI CTAG 4 cut(s) 260, 653, 683, 979
GlaI GCGC 1 cut(s) 155
GluI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
GsuI CTGGAG 1 cut(s) 502
HaeII RGCGCY 1 cut(s) 157
HapII CCGG 1 cut(s) 973
HgaI GACGC 2 cut(s) 173, 877
HhaI GCGC 1 cut(s) 156
Hin1I GRCGYC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 3 cut(s) 29, 104, 758
HpaII CCGG 1 cut(s) 973
HphI GGTGA 2 cut(s) 259, 344
Hpy166II GTNNAC 2 cut(s) 488, 691
Hpy188I TCNGA 7 cut(s) 34, 79, 208, 220, 270, 445, 550
Hpy188III TCNNGA 8 cut(s) 118, 140, 314, 524, 611, 653, 725, 973
Hpy8I GTNNAC 2 cut(s) 488, 691
Hpy99I CGWCG 5 cut(s) 98, 146, 149, 167, 871
HpyAV CCTTC 3 cut(s) 236, 569, 608
HpyCH4III ACNGT 3 cut(s) 215, 771, 961
HpyCH4IV ACGT 1 cut(s) 949
HpyCH4V TGCA 5 cut(s) 287, 293, 393, 721, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 290, 962
HpySE526I ACGT 1 cut(s) 949
Hsp92I GRCGYC 1 cut(s) 165
HspAI GCGC 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 972
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 5 cut(s) 67, 445, 607, 798, 935
LmnI GCTCC 2 cut(s) 95, 347
Lsp1109I GCAGC 4 cut(s) 55, 68, 172, 271
LweI GCATC 4 cut(s) 226, 271, 697, 730
MaeI CTAG 4 cut(s) 260, 653, 683, 979
MaeII ACGT 1 cut(s) 949
MaeIII GTNAC 3 cut(s) 247, 809, 945
MalI GATC 5 cut(s) 69, 447, 609, 800, 937
MboI GATC 5 cut(s) 67, 445, 607, 798, 935
MboII GAAGA 2 cut(s) 257, 512
MflI RGATCY 1 cut(s) 935
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 4 cut(s) 307, 632, 747, 827
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 3 cut(s) 645, 703, 941
MnlI CCTC 6 cut(s) 166, 188, 203, 214, 220, 382
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 1 cut(s) 972
MseI TTAA 2 cut(s) 554, 630
MslI CAYNNNNRTG 3 cut(s) 330, 362, 726
MspI CCGG 1 cut(s) 973
MwoI GCNNNNNNNGC 3 cut(s) 167, 290, 962
NdeI CATATG 1 cut(s) 289
NdeII GATC 5 cut(s) 67, 445, 607, 798, 935
NlaIV GGNNCC 3 cut(s) 231, 700, 937
NmeAIII GCCGAG 2 cut(s) 77, 196
NmuCI GTSAC 2 cut(s) 247, 945
NsiI ATGCAT 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 2 cut(s) 104, 758
PkrI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
PleI GAGTC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 950
PmlI CACGTG 1 cut(s) 950
PpsI GAGTC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 950
PpuMI RGGWCCY 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 175
PspCI CACGTG 1 cut(s) 950
PspN4I GGNNCC 3 cut(s) 231, 700, 937
PspPI GGNCC 3 cut(s) 111, 175, 378
PspPPI RGGWCCY 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 239, 563
PsuI RGATCY 1 cut(s) 935
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 3 cut(s) 330, 362, 726
SaqAI TTAA 2 cut(s) 554, 630
SatI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
Sau3AI GATC 5 cut(s) 67, 445, 607, 798, 935
Sau96I GGNCC 3 cut(s) 111, 175, 378
SchI GAGTC 1 cut(s) 23
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 4 cut(s) 226, 271, 697, 730
SinI GGWCC 3 cut(s) 111, 175, 378
SmiMI CAYNNNNRTG 3 cut(s) 330, 362, 726
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 4 cut(s) 307, 632, 747, 827
SsiI CCGC 4 cut(s) 62, 99, 168, 907
SspMI CTAG 4 cut(s) 260, 653, 683, 979
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 3 cut(s) 215, 771, 961
TaiI ACGT 1 cut(s) 952
TaqI TCGA 3 cut(s) 13, 141, 595
TasI AATT 4 cut(s) 307, 632, 747, 827
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 101, 170
TfiI GAWTC 2 cut(s) 104, 758
Tru1I TTAA 2 cut(s) 554, 630
Tru9I TTAA 2 cut(s) 554, 630
TscAI CASTG 1 cut(s) 964
TseFI GTSAC 2 cut(s) 247, 945
TseI GCWGC 4 cut(s) 43, 56, 185, 284
Tsp45I GTSAC 2 cut(s) 247, 945
TspDTI ATGAA 5 cut(s) 96, 99, 840, 894, 913
TspGWI ACGGA 3 cut(s) 82, 185, 928
TspRI CASTG 1 cut(s) 964
VpaK11BI GGWCC 3 cut(s) 111, 175, 378
XapI RAATTY 2 cut(s) 307, 632
XbaI TCTAGA 1 cut(s) 652
XspI CTAG 4 cut(s) 260, 653, 683, 979
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.