Rw3G027390

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0014371
Physical Location & Seq
Reverse (-)
38808817 .. 38813199
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027390.1

Sequence Viewer

Length: 1008 bp
ATGGCTGGAATCCGATTGGGCCCTGATGACTCAGAGATATCGCAGCAGAGCCGAGCTGCTCCGGCCGATCTTATCTCAGACGACGATCGCTCCGTCGCCGCGGACTCGTGGTCCATCAAGAGCGACTATGGGAGCACTCTCGACGACGACCAACGCCACGCTGATGCTGCTGAGGATCTCTCCGCCGCTAACCTCCGCGCCCCCTCCGATTACAGCTCTGACAAGGAGGAACCGGATTCTGATGGGATGACATCAATGCTAGGCCTTCAGAGTTATTGGGATGCTGCCTATGCAGATGAGCTGGTTAATTTTCGTGAACATGGGCATGCTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTTGCCTCTTGGACCAAAACATTATGCATTGATGTTTCTCAAGGTCGCATGCCAAATCATGTTGATAATAGCAAGTCTGAATCAGCTGAACAAGGTTACAAGTTTCTTTCAGGTTGGAGTGTACTTGACATTGGGACTGGCAATGGATTGCTTCTTCAAGAACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAATTGATTATAGTGAAGGGGCGATTGACCTTGCTCGAAACCTTGCTGACCGTGATGGGTTACCCAATATTCATTTTTTGGTTGATGATATCCTAGAGACGAAGTTGGAACGACAGTTTCAGCTTGTTGTAGACAAAGGAACCTTAGATGCCATTGGATTGCATCCAGATGGTGCTATAAAAAGGTTAATATATTGGGATTCAGTTTCGAGATTGATGGCTCCTGGTGGAATATTAGTAATCACATCATGTAACAGCACTAAAGAAGAATTAGTACAAGAAGTGGAAAATTTCAATCAAAGAAGGATTGGTGTGACCCAAGATCAAGAAGGATGCAAAGATACGGCTCCTCAGCTTCAATACCTTAGTCATGTTCGCACATATCCAACATTCATGTTTGGTGGATCTGTAGGATCACGGGTTGCCACTGTGGCATTTCTCCGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

335

Amino Acids

36.69

Weight (kDa)

4.44

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 159 - 285 2e-20 Methyltransferase domain
Methyltransf_23 PF13489 159 - 273 5e-10 Methyltransferase domain
MTS PF05175 162 - 268 7.6e-06 Methyltransferase small domain
Methyltransf_25 PF13649 163 - 238 7.6e-13 Methyltransferase domain
Methyltransf_12 PF08242 164 - 265 6.3e-10 Methyltransferase domain
Methyltransf_11 PF08241 164 - 267 5.4e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 690
AccII CGCG 2 cut(s) 101, 198
AccIII TCCGGA 1 cut(s) 999
AciI CCGC 5 cut(s) 99, 101, 183, 186, 196
AclWI GGATC 3 cut(s) 183, 970, 979
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 847
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 2 cut(s) 489, 834
AfiI CCNNNNNNNGG 1 cut(s) 540
AgsI TTSAA 3 cut(s) 524, 853, 917
AhdI GACNNNNNGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 781
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 7 cut(s) 56, 216, 301, 350, 452, 682, 913
AluI AGCT 7 cut(s) 56, 216, 301, 350, 452, 682, 913
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 3 cut(s) 183, 970, 979
Aor13HI TCCGGA 1 cut(s) 999
AoxI GGCC 3 cut(s) 19, 63, 262
ApaI GGGCCC 1 cut(s) 23
ApeKI GCWGC 4 cut(s) 43, 56, 167, 284
ApoI RAATTY 1 cut(s) 847
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 4 cut(s) 19, 20, 111, 378
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 2 cut(s) 111, 378
BaeGI GKGCMC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 816, 848
BauI CACGAG 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 2 cut(s) 171, 909
BbvI GCAGC 4 cut(s) 43, 55, 154, 271
BccI CCATC 5 cut(s) 122, 236, 608, 722, 769
BceAI ACGGC 1 cut(s) 918
BciT130I CCWGG 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 2 cut(s) 260, 653
BfmI CTRYAG 1 cut(s) 966
BglI GCCNNNNNGGC 1 cut(s) 989
BisI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
BlsI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 2 cut(s) 111, 378
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 4 cut(s) 19, 20, 111, 378
BmiI GGNNCC 5 cut(s) 21, 231, 700, 780, 906
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 5 cut(s) 154, 271, 697, 730, 881
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu10I CCTNAGC 2 cut(s) 171, 909
BpuEI CTTGAG 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 2 cut(s) 232, 999
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 540
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 514
BseAI TCCGGA 1 cut(s) 999
BseBI CCWGG 1 cut(s) 783
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 4 cut(s) 252, 286, 721, 896
BseLI CCNNNNNNNGG 1 cut(s) 540
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 4 cut(s) 45, 90, 162, 923
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 897
BseSI GKGCMC 1 cut(s) 23
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 43, 55, 154, 271
Bsh1236I CGCG 2 cut(s) 101, 198
Bsh1285I CGRYCG 2 cut(s) 66, 88
BshFI GGCC 3 cut(s) 21, 65, 264
BsiEI CGRYCG 2 cut(s) 66, 88
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 3 cut(s) 62, 233, 1000
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 540
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 21, 65, 264
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 23, 137
Bsp13I TCCGGA 1 cut(s) 999
Bsp143I GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BspACI CCGC 5 cut(s) 99, 101, 183, 186, 196
BspANI GGCC 3 cut(s) 21, 65, 264
BspCNI CTCAG 4 cut(s) 44, 89, 163, 922
BspEI TCCGGA 1 cut(s) 999
BspFNI CGCG 2 cut(s) 101, 198
BspLI GGNNCC 5 cut(s) 21, 231, 700, 780, 906
BspPI GGATC 3 cut(s) 183, 970, 979
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 3 cut(s) 611, 675, 988
BstC8I GCNNGC 2 cut(s) 327, 416
BstDEI CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
BstDSI CCRYGG 1 cut(s) 99
BstEII GGTNACC 1 cut(s) 618
BstF5I GGATG 4 cut(s) 252, 286, 721, 896
BstFNI CGCG 2 cut(s) 101, 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 6 cut(s) 70, 88, 178, 883, 965, 974
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
BstMCI CGRYCG 2 cut(s) 66, 88
BstMWI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
BstNI CCWGG 1 cut(s) 783
BstNSI RCATGY 2 cut(s) 329, 418
BstPI GGTNACC 1 cut(s) 618
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 1 cut(s) 966
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 2 cut(s) 101, 198
BstV1I GCAGC 4 cut(s) 43, 55, 154, 271
BstX2I RGATCY 2 cut(s) 175, 962
BstYI RGATCY 2 cut(s) 175, 962
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 21, 65, 264
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 4 cut(s) 252, 286, 721, 896
BtsIMutI CAGTG 1 cut(s) 984
Cac8I GCNNGC 2 cut(s) 327, 416
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 4 cut(s) 19, 20, 111, 378
Cfr42I CCGCGG 1 cut(s) 102
Csp6I GTAC 2 cut(s) 488, 833
CviAII CATG 8 cut(s) 320, 326, 358, 415, 425, 807, 929, 952
CviQI GTAC 2 cut(s) 488, 833
DdeI CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
DpnI GATC 6 cut(s) 69, 87, 177, 882, 964, 973
DpnII GATC 6 cut(s) 67, 85, 175, 880, 962, 971
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 2 cut(s) 39, 649
Eco47I GGWCC 2 cut(s) 111, 378
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 20
EcoO65I GGTNACC 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 781
EcoRV GATATC 2 cut(s) 39, 649
EcoT22I ATGCAT 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
FaqI GGGAC 1 cut(s) 514
FatI CATG 8 cut(s) 319, 325, 357, 414, 424, 806, 928, 951
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FokI GGATG 4 cut(s) 259, 293, 708, 903
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FspBI CTAG 2 cut(s) 260, 653
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
HaeIII GGCC 3 cut(s) 21, 65, 264
HapII CCGG 3 cut(s) 62, 233, 1000
HhaI GCGC 1 cut(s) 200
Hin1II CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 6 cut(s) 9, 29, 104, 236, 446, 758
HpaII CCGG 3 cut(s) 62, 233, 1000
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 317, 488, 691
Hpy188I TCNGA 9 cut(s) 14, 34, 79, 208, 220, 241, 270, 445, 550
Hpy188III TCNNGA 8 cut(s) 118, 140, 314, 524, 725, 768, 884, 1000
Hpy8I GTNNAC 3 cut(s) 317, 488, 691
Hpy99I CGWCG 4 cut(s) 86, 98, 146, 149
HpyAV CCTTC 4 cut(s) 275, 569, 855, 881
HpyCH4III ACNGT 3 cut(s) 611, 675, 988
HpyCH4V TGCA 4 cut(s) 293, 393, 721, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
HpyF3I CTNAG 6 cut(s) 31, 76, 171, 703, 909, 923
Hsp92II CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
HspAI GCGC 1 cut(s) 198
Kpn2I TCCGGA 1 cut(s) 999
KspI CCGCGG 1 cut(s) 102
Kzo9I GATC 6 cut(s) 67, 85, 175, 880, 962, 971
LmnI GCTCC 6 cut(s) 64, 95, 132, 347, 784, 910
Lsp1109I GCAGC 4 cut(s) 43, 55, 154, 271
LweI GCATC 5 cut(s) 154, 271, 697, 730, 881
MaeI CTAG 2 cut(s) 260, 653
MaeIII GTNAC 4 cut(s) 461, 618, 809, 871
MalI GATC 6 cut(s) 69, 87, 177, 882, 964, 973
MboI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
MboII GAAGA 2 cut(s) 512, 836
MflI RGATCY 2 cut(s) 175, 962
MhlI GDGCHC 2 cut(s) 23, 137
MluCI AATT 5 cut(s) 307, 561, 827, 847, 1003
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 3 cut(s) 461, 645, 968
MnlI CCTC 6 cut(s) 166, 203, 214, 220, 382, 918
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 1 cut(s) 999
MseI TTAA 3 cut(s) 306, 554, 746
MslI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
MspA1I CMGCKG 2 cut(s) 101, 452
MspI CCGG 3 cut(s) 62, 233, 1000
MspR9I CCNGG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 783
MvnI CGCG 2 cut(s) 101, 198
MwoI GCNNNNNNNGC 4 cut(s) 62, 167, 290, 989
NdeII GATC 6 cut(s) 67, 85, 175, 880, 962, 971
NlaIII CATG 8 cut(s) 323, 329, 361, 418, 428, 810, 932, 955
NlaIV GGNNCC 5 cut(s) 21, 231, 700, 780, 906
NmeAIII GCCGAG 1 cut(s) 77
NmuCI GTSAC 1 cut(s) 871
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 2 cut(s) 329, 418
PaeI GCATGC 2 cut(s) 329, 418
PceI AGGCCT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 4 cut(s) 9, 236, 446, 758
PkrI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
Psp6I CCWGG 1 cut(s) 781
PspEI GGTNACC 1 cut(s) 618
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 5 cut(s) 21, 231, 700, 780, 906
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 4 cut(s) 19, 20, 111, 378
PsuI RGATCY 2 cut(s) 175, 962
PvuI CGATCG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 489, 834
RsaNI GTAC 2 cut(s) 488, 833
RseI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SacII CCGCGG 1 cut(s) 102
SaqAI TTAA 3 cut(s) 306, 554, 746
SatI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
Sau3AI GATC 6 cut(s) 67, 85, 175, 880, 962, 971
Sau96I GGNCC 4 cut(s) 19, 20, 111, 378
SchI GAGTC 2 cut(s) 23, 98
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 2 cut(s) 23, 137
SfaNI GCATC 5 cut(s) 154, 271, 697, 730, 881
SfcI CTRYAG 1 cut(s) 966
Sfr303I CCGCGG 1 cut(s) 102
SgrBI CCGCGG 1 cut(s) 102
SinI GGWCC 2 cut(s) 111, 378
SmiMI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 2 cut(s) 329, 418
Sse9I AATT 5 cut(s) 307, 561, 827, 847, 1003
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 5 cut(s) 99, 101, 183, 186, 196
SspI AATATT 2 cut(s) 628, 792
SspMI CTAG 2 cut(s) 260, 653
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 781
TaaI ACNGT 3 cut(s) 611, 675, 988
TaqI TCGA 3 cut(s) 141, 595, 767
TasI AATT 5 cut(s) 307, 561, 827, 847, 1003
TatI WGTACW 2 cut(s) 487, 832
TauI GCSGC 2 cut(s) 101, 188
TfiI GAWTC 4 cut(s) 9, 236, 446, 758
Tru1I TTAA 3 cut(s) 306, 554, 746
Tru9I TTAA 3 cut(s) 306, 554, 746
TscAI CASTG 1 cut(s) 991
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 4 cut(s) 43, 56, 167, 284
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 2 cut(s) 620, 940
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 1 cut(s) 991
VpaK11BI GGWCC 2 cut(s) 111, 378
XapI RAATTY 1 cut(s) 847
XceI RCATGY 2 cut(s) 329, 418
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 2 cut(s) 260, 653
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.