Prupe.3G150900_v2.0.a1

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014371
Physical Location & Seq
Forward (+)
16518839 .. 16528425
9587 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G150900.1

Sequence Viewer

Length: 996 bp
ATGGCTGGACTTCGATTGGGGCCAGATGACTCGGAGCTATCGCAGCAGAGCCGAGCTGCTCCGGCCGATCTCATCTCCGACGACGACCGCTCCGTCGCCGCAGACTCATGGTCCATCAAGAGTGACTACGGAAGCACTCTCGACGACGACCAACGCCACGCCGACGCTGCCGAGGCCCTCTCCGCCGCTAACCTCCGCGCCCCCTCCGATTACAGTTCTGACAAGGAGGAACCAGATGCTGAAGGTGTGACATCAATGCTAGGTCTTCAGAGTTATTGGGATGCTGCATATGCAGATGAGTTGGCGAATTTTCGTGAACATGGTCATACTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTCGGCTCTTGGACCAAAAACTTATGCATTGATATTTCTCGAGGTCGCATGCCAAATCATGTTGATAATATCAAGTCTGATCCTGTTGAACAAGGTGATAAGTATCTTTCAGATTGGAGTGTACTTGACATTGGGACTGGCAATGGATTGCTACTTCAAGAACTTTCCAAGCAAGGGTTTTCGGATTTAACTGGAACCGATTATAGTGAAGGAGCGATTGACCTTGCTCGAAGCCTTGCAGGTCGTGTTGGATTACCCAACATTCATTTTTTGGTTGATGATGTTCTAGACACCAAGTTGGAAAGGCAGTTTCAACTTGTTGTGGACAAAGGAACCTTAGATGCCATTGGATTGCATCCAGATGGTTCTATCAAAAGGGTAATGTATTGGGATTCAGTTTCAAGATTGGTGGCTCCTGGCGGAGTATTGGTGATCACATCATGTAACAGCACAAAAGATGAATTGGTGCAAGAAGTGGAAAGTTTCAATCAAAGGAGTGTGACCCAGGAACATGATGAAACACCCAAAGATAAGTTCCAATACCTTAGTCATGTCCGCACATATCCGACATTCATGTTCGGTGGATCCGTAGGATCACGTGTTGCCACTGTGGCATTTCTCCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

332

Amino Acids

36.23

Weight (kDa)

4.5

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 596
AccBSI CCGCTC 1 cut(s) 90
AccII CGCG 1 cut(s) 198
AciI CCGC 7 cut(s) 88, 99, 183, 186, 196, 786, 922
AclWI GGATC 4 cut(s) 440, 945, 958, 967
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 2 cut(s) 251, 261
AcvI CACGTG 1 cut(s) 965
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 1 cut(s) 540
AflIII ACRYGT 1 cut(s) 964
AgsI TTSAA 5 cut(s) 455, 524, 680, 768, 853
AhdI GACNNNNNGTC 1 cut(s) 109
AjnI CCWGG 2 cut(s) 781, 870
AjuI GAANNNNNNNTTGG 4 cut(s) 327, 359, 884, 916
AluBI AGCT 3 cut(s) 37, 56, 350
AluI AGCT 3 cut(s) 37, 56, 350
AlwI GGATC 4 cut(s) 440, 945, 958, 967
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 3 cut(s) 20, 63, 174
ApeKI GCWGC 4 cut(s) 43, 56, 167, 284
ApoI RAATTY 1 cut(s) 307
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 4 cut(s) 20, 111, 175, 378
AsuHPI GGTGA 3 cut(s) 344, 473, 808
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 2 cut(s) 111, 378
BamHI GGATCC 1 cut(s) 950
BbrPI CACGTG 1 cut(s) 965
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 43, 55, 154, 271
BccI CCATC 2 cut(s) 122, 722
BciT130I CCWGG 2 cut(s) 783, 872
BclI TGATCA 1 cut(s) 798
BfaI CTAG 2 cut(s) 260, 653
BfuAI ACCTGC 1 cut(s) 596
BglI GCCNNNNNGGC 1 cut(s) 977
BisI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
BlsI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Bme1390I CCNGG 2 cut(s) 783, 872
Bme18I GGWCC 2 cut(s) 111, 378
BmeRI GACNNNNNGTC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 4 cut(s) 20, 111, 175, 378
BmiI GGNNCC 6 cut(s) 21, 231, 562, 700, 780, 952
BmrFI CCNGG 2 cut(s) 783, 872
BmsI GCATC 4 cut(s) 226, 271, 697, 730
BpiI GAAGAC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BsaAI YACGTR 1 cut(s) 965
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 171, 870
BsaXI ACNNNNNCTCC 6 cut(s) 74, 104, 339, 369, 780, 810
Bsc4I CCNNNNNNNGG 1 cut(s) 540
Bse1I ACTGG 3 cut(s) 334, 508, 562
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 2 cut(s) 783, 872
BseDI CCNNGG 2 cut(s) 171, 870
BseGI GGATG 2 cut(s) 286, 721
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 540
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 3 cut(s) 334, 508, 562
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 43, 55, 154, 271
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 2 cut(s) 66, 88
BshFI GGCC 3 cut(s) 22, 65, 176
BsiEI CGRYCG 2 cut(s) 66, 88
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 540
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 22, 65, 176
BsoBI CYCGRG 1 cut(s) 405
Bsp143I GATC 5 cut(s) 67, 445, 798, 950, 959
BspACI CCGC 7 cut(s) 88, 99, 183, 186, 196, 786, 922
BspANI GGCC 3 cut(s) 22, 65, 176
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 6 cut(s) 21, 231, 562, 700, 780, 952
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 4 cut(s) 440, 945, 958, 967
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 3 cut(s) 334, 508, 562
BssECI CCNNGG 2 cut(s) 171, 870
BssMI GATC 5 cut(s) 67, 445, 798, 950, 959
Bst2UI CCWGG 2 cut(s) 783, 872
Bst4CI ACNGT 2 cut(s) 215, 976
BstBAI YACGTR 1 cut(s) 965
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 703, 911
BstF5I GGATG 2 cut(s) 286, 721
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 5 cut(s) 70, 448, 801, 953, 962
BstMBI GATC 5 cut(s) 67, 445, 798, 950, 959
BstMCI CGRYCG 2 cut(s) 66, 88
BstMWI GCNNNNNNNGC 7 cut(s) 43, 62, 167, 173, 182, 290, 977
BstNI CCWGG 2 cut(s) 783, 872
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 2 cut(s) 781, 870
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 43, 55, 154, 271
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 22, 65, 176
BtsCI GGATG 2 cut(s) 286, 721
BtsIMutI CAGTG 1 cut(s) 972
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 1 cut(s) 416
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 4 cut(s) 20, 111, 175, 378
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 488
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 9 cut(s) 108, 320, 358, 415, 425, 807, 878, 917, 940
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 2 cut(s) 703, 911
DpnI GATC 5 cut(s) 69, 447, 800, 952, 961
DpnII GATC 5 cut(s) 67, 445, 798, 950, 959
DrdI GACNNNNNNGTC 1 cut(s) 92
DriI GACNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 109
EciI GGCGGA 2 cut(s) 172, 801
EclXI CGGCCG 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 111, 378
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 251, 261
Eco72I CACGTG 1 cut(s) 965
Eco88I CYCGRG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 781, 870
EcoT22I ATGCAT 1 cut(s) 395
FaeI CATG 9 cut(s) 111, 323, 361, 418, 428, 810, 881, 920, 943
FaqI GGGAC 1 cut(s) 514
FatI CATG 9 cut(s) 107, 319, 357, 414, 424, 806, 877, 916, 939
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FokI GGATG 2 cut(s) 293, 708
Fsp4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FspBI CTAG 2 cut(s) 260, 653
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
HaeIII GGCC 3 cut(s) 22, 65, 176
HapII CCGG 1 cut(s) 62
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 200
Hin1II CATG 9 cut(s) 111, 323, 361, 418, 428, 810, 881, 920, 943
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 3 cut(s) 29, 104, 758
HpaII CCGG 1 cut(s) 62
HphI GGTGA 3 cut(s) 344, 473, 808
Hpy166II GTNNAC 3 cut(s) 317, 488, 691
Hpy188III TCNNGA 8 cut(s) 118, 140, 314, 405, 524, 653, 725, 768
Hpy8I GTNNAC 3 cut(s) 317, 488, 691
Hpy99I CGWCG 6 cut(s) 83, 86, 98, 146, 149, 167
HpyAV CCTTC 2 cut(s) 236, 569
HpyCH4III ACNGT 2 cut(s) 215, 976
HpyCH4IV ACGT 1 cut(s) 964
HpyCH4V TGCA 6 cut(s) 287, 293, 393, 605, 721, 835
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 62, 167, 173, 182, 290, 977
HpyF3I CTNAG 2 cut(s) 703, 911
HpySE526I ACGT 1 cut(s) 964
Hsp92II CATG 9 cut(s) 111, 323, 361, 418, 428, 810, 881, 920, 943
HspAI GCGC 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 5 cut(s) 67, 445, 798, 950, 959
LmnI GCTCC 6 cut(s) 34, 64, 95, 347, 578, 784
Lsp1109I GCAGC 4 cut(s) 43, 55, 154, 271
LweI GCATC 4 cut(s) 226, 271, 697, 730
MaeI CTAG 2 cut(s) 260, 653
MaeII ACGT 1 cut(s) 964
MaeIII GTNAC 4 cut(s) 122, 247, 809, 865
MalI GATC 5 cut(s) 69, 447, 800, 952, 961
MbiI CCGCTC 1 cut(s) 90
MboI GATC 5 cut(s) 67, 445, 798, 950, 959
MboII GAAGA 1 cut(s) 257
MflI RGATCY 1 cut(s) 950
MluCI AATT 2 cut(s) 307, 827
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 4 cut(s) 102, 595, 645, 956
MnlI CCTC 6 cut(s) 166, 188, 203, 214, 220, 401
Mph1103I ATGCAT 1 cut(s) 395
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 2 cut(s) 330, 726
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 2 cut(s) 783, 872
MvaI CCWGG 2 cut(s) 783, 872
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 7 cut(s) 43, 62, 167, 173, 182, 290, 977
NdeI CATATG 1 cut(s) 289
NdeII GATC 5 cut(s) 67, 445, 798, 950, 959
NlaIII CATG 9 cut(s) 111, 323, 361, 418, 428, 810, 881, 920, 943
NlaIV GGNNCC 6 cut(s) 21, 231, 562, 700, 780, 952
NmeAIII GCCGAG 2 cut(s) 77, 196
NmuCI GTSAC 3 cut(s) 122, 247, 865
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 1 cut(s) 418
PaeI GCATGC 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 405
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 951
PfeI GAWTC 1 cut(s) 758
PkrI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
PleI GAGTC 2 cut(s) 23, 98
PmaCI CACGTG 1 cut(s) 965
PmlI CACGTG 1 cut(s) 965
PpsI GAGTC 2 cut(s) 23, 98
Ppu21I YACGTR 1 cut(s) 965
Psp6I CCWGG 2 cut(s) 781, 870
PspCI CACGTG 1 cut(s) 965
PspGI CCWGG 2 cut(s) 781, 870
PspN4I GGNNCC 6 cut(s) 21, 231, 562, 700, 780, 952
PspPI GGNCC 4 cut(s) 20, 111, 175, 378
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 950
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 2 cut(s) 330, 726
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
Sau3AI GATC 5 cut(s) 67, 445, 798, 950, 959
Sau96I GGNCC 4 cut(s) 20, 111, 175, 378
SchI GAGTC 2 cut(s) 23, 98
ScrFI CCNGG 2 cut(s) 783, 872
SfaNI GCATC 4 cut(s) 226, 271, 697, 730
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 2 cut(s) 111, 378
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 2 cut(s) 330, 726
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 1 cut(s) 418
Sse9I AATT 2 cut(s) 307, 827
SsiI CCGC 7 cut(s) 88, 99, 183, 186, 196, 786, 922
SspMI CTAG 2 cut(s) 260, 653
StyD4I CCNGG 2 cut(s) 781, 870
TaaI ACNGT 2 cut(s) 215, 976
TaiI ACGT 1 cut(s) 967
TaqI TCGA 4 cut(s) 13, 141, 406, 595
TasI AATT 2 cut(s) 307, 827
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 101, 188
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 1 cut(s) 979
TseFI GTSAC 3 cut(s) 122, 247, 865
TseI GCWGC 4 cut(s) 43, 56, 167, 284
Tsp45I GTSAC 3 cut(s) 122, 247, 865
TspDTI ATGAA 4 cut(s) 620, 840, 897, 928
TspGWI ACGGA 3 cut(s) 82, 144, 943
TspRI CASTG 1 cut(s) 979
VpaK11BI GGWCC 2 cut(s) 111, 378
XapI RAATTY 1 cut(s) 307
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 1 cut(s) 418
XhoI CTCGAG 1 cut(s) 405
XspI CTAG 2 cut(s) 260, 653
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.