Rh3AG312200

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0014371
Physical Location & Seq
Reverse (-)
39299250 .. 39303433
4184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG312200.1

Sequence Viewer

Length: 747 bp
ATGGCTGGAATCCGATTGGGCCCTGATGACTCAGAGATATCGCAGCAGAGCCGAGCTGCTCCGGCCGATCTTATCTCAGACGACGATCGCTCCGTCGCCGCGGACTCGTGGTCCATCAAGAGCGACTATGGGAGCACTCTCGACGACGACCAACGCCACGCTGATGCTGCTGAGGATCTCTCCGCCGCTAACCTCCGCGCCCCCTCCGATTACAGCTCTGACAAGGAGGAACCAGATTCTGATGGGATGACATCAATGCTAGGCCTTCAGAGTTATTGGGATGCTGCCTATGCAGATGAGCTGGTTAATTTTCGTGAACATGGGCATGCTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTTGCCTCTTGGACCAAAACATTATGCATTGATGTTTCTCAAGGTCGCATGCCAAATCATGTTGATAATAGCAAGTCTGAATCAGCTGAACAAGGTTACAAGTTTCTTTCAGGTTGGAGTGTACTTGACATTGGGACCGGCAATGGATTGCTTCTTCAAGAACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAATTGATTATAGTGAAGGGGCGATTGACCTTGCTCGAAACCTTGCTGACCGTGATGGATTACCCAATATTCATTTTTTGGTTGATGATATTCTAGAGACGAAGTTGGAACGACAGTTTCAGCTTGTTGTAGACAAAGGAACCTTAGATGCCATTGGATTGCATCCAGATGGTGCTATAAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

248

Amino Acids

26.98

Weight (kDa)

4.27

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 159 - 239 8.1e-15 Methyltransferase domain
Methyltransf_23 PF13489 160 - 238 1.1e-07 Methyltransferase domain
Methyltransf_25 PF13649 163 - 235 1.1e-12 Methyltransferase domain
Methyltransf_11 PF08241 164 - 238 1.5e-08 Methyltransferase domain
Methyltransf_12 PF08242 164 - 245 3.3e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 690
AccII CGCG 2 cut(s) 101, 198
AciI CCGC 5 cut(s) 99, 101, 183, 186, 196
AclWI GGATC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 1 cut(s) 540
AgsI TTSAA 1 cut(s) 524
AhdI GACNNNNNGTC 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 6 cut(s) 56, 216, 301, 350, 452, 682
AluI AGCT 6 cut(s) 56, 216, 301, 350, 452, 682
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 183
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 3 cut(s) 19, 63, 262
ApaI GGGCCC 1 cut(s) 23
ApeKI GCWGC 4 cut(s) 43, 56, 167, 284
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 5 cut(s) 19, 20, 111, 378, 501
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 3 cut(s) 111, 378, 501
BaeGI GKGCMC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 23
BauI CACGAG 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 4 cut(s) 43, 55, 154, 271
BccI CCATC 4 cut(s) 122, 236, 608, 722
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 2 cut(s) 260, 653
BisI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
BlsI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Bme18I GGWCC 3 cut(s) 111, 378, 501
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 5 cut(s) 19, 20, 111, 378, 501
BmiI GGNNCC 4 cut(s) 21, 231, 502, 700
BmsI GCATC 4 cut(s) 154, 271, 697, 730
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 540
Bse118I RCCGGY 1 cut(s) 503
Bse3DI GCAATG 1 cut(s) 514
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 3 cut(s) 252, 286, 721
BseLI CCNNNNNNNGG 1 cut(s) 540
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 3 cut(s) 45, 90, 162
BseSI GKGCMC 1 cut(s) 23
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 43, 55, 154, 271
Bsh1236I CGCG 2 cut(s) 101, 198
Bsh1285I CGRYCG 2 cut(s) 66, 88
BshFI GGCC 3 cut(s) 21, 65, 264
BsiEI CGRYCG 2 cut(s) 66, 88
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 2 cut(s) 62, 504
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 540
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 21, 65, 264
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 23, 137
Bsp143I GATC 3 cut(s) 67, 85, 175
BspACI CCGC 5 cut(s) 99, 101, 183, 186, 196
BspANI GGCC 3 cut(s) 21, 65, 264
BspCNI CTCAG 3 cut(s) 44, 89, 163
BspFNI CGCG 2 cut(s) 101, 198
BspLI GGNNCC 4 cut(s) 21, 231, 502, 700
BspPI GGATC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 503
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 3 cut(s) 67, 85, 175
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 611, 675
BstC8I GCNNGC 2 cut(s) 327, 416
BstDEI CTNAG 4 cut(s) 31, 76, 171, 703
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 252, 286, 721
BstFNI CGCG 2 cut(s) 101, 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 3 cut(s) 70, 88, 178
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 67, 85, 175
BstMCI CGRYCG 2 cut(s) 66, 88
BstMWI GCNNNNNNNGC 3 cut(s) 62, 167, 290
BstNSI RCATGY 2 cut(s) 329, 418
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 2 cut(s) 101, 198
BstV1I GCAGC 4 cut(s) 43, 55, 154, 271
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 21, 65, 264
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 3 cut(s) 252, 286, 721
Cac8I GCNNGC 2 cut(s) 327, 416
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 5 cut(s) 19, 20, 111, 378, 501
Cfr42I CCGCGG 1 cut(s) 102
Csp6I GTAC 1 cut(s) 488
CviAII CATG 5 cut(s) 320, 326, 358, 415, 425
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 4 cut(s) 31, 76, 171, 703
DpnI GATC 3 cut(s) 69, 87, 177
DpnII GATC 3 cut(s) 67, 85, 175
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 111, 378, 501
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 20
EcoRV GATATC 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 5 cut(s) 323, 329, 361, 418, 428
FaqI GGGAC 1 cut(s) 514
FatI CATG 5 cut(s) 319, 325, 357, 414, 424
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FokI GGATG 3 cut(s) 259, 293, 708
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
FspBI CTAG 2 cut(s) 260, 653
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
HaeIII GGCC 3 cut(s) 21, 65, 264
HapII CCGG 2 cut(s) 62, 504
HhaI GCGC 1 cut(s) 200
Hin1II CATG 5 cut(s) 323, 329, 361, 418, 428
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 5 cut(s) 9, 29, 104, 236, 446
HpaII CCGG 2 cut(s) 62, 504
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 317, 488, 691
Hpy188I TCNGA 9 cut(s) 14, 34, 79, 208, 220, 241, 270, 445, 550
Hpy188III TCNNGA 6 cut(s) 118, 140, 314, 524, 653, 725
Hpy8I GTNNAC 3 cut(s) 317, 488, 691
Hpy99I CGWCG 4 cut(s) 86, 98, 146, 149
HpyAV CCTTC 2 cut(s) 275, 569
HpyCH4III ACNGT 2 cut(s) 611, 675
HpyCH4V TGCA 3 cut(s) 293, 393, 721
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 167, 290
HpyF3I CTNAG 4 cut(s) 31, 76, 171, 703
Hsp92II CATG 5 cut(s) 323, 329, 361, 418, 428
HspAI GCGC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 102
Kzo9I GATC 3 cut(s) 67, 85, 175
LmnI GCTCC 4 cut(s) 64, 95, 132, 347
Lsp1109I GCAGC 4 cut(s) 43, 55, 154, 271
LweI GCATC 4 cut(s) 154, 271, 697, 730
MaeI CTAG 2 cut(s) 260, 653
MaeIII GTNAC 1 cut(s) 461
MalI GATC 3 cut(s) 69, 87, 177
MboI GATC 3 cut(s) 67, 85, 175
MboII GAAGA 1 cut(s) 512
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 23, 137
MluCI AATT 2 cut(s) 307, 561
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 2 cut(s) 461, 645
MnlI CCTC 5 cut(s) 166, 203, 214, 220, 382
Mph1103I ATGCAT 1 cut(s) 395
MseI TTAA 2 cut(s) 306, 554
MslI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
MspA1I CMGCKG 2 cut(s) 101, 452
MspI CCGG 2 cut(s) 62, 504
MvnI CGCG 2 cut(s) 101, 198
MwoI GCNNNNNNNGC 3 cut(s) 62, 167, 290
NdeII GATC 3 cut(s) 67, 85, 175
NlaIII CATG 5 cut(s) 323, 329, 361, 418, 428
NlaIV GGNNCC 4 cut(s) 21, 231, 502, 700
NmeAIII GCCGAG 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 2 cut(s) 329, 418
PaeI GCATGC 2 cut(s) 329, 418
PceI AGGCCT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 3 cut(s) 9, 236, 446
PkrI GCNGC 6 cut(s) 45, 58, 100, 169, 187, 286
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
PspN4I GGNNCC 4 cut(s) 21, 231, 502, 700
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 5 cut(s) 19, 20, 111, 378, 501
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 175
PvuI CGATCG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SacII CCGCGG 1 cut(s) 102
SaqAI TTAA 2 cut(s) 306, 554
SatI GCNGC 6 cut(s) 44, 57, 99, 168, 186, 285
Sau3AI GATC 3 cut(s) 67, 85, 175
Sau96I GGNCC 5 cut(s) 19, 20, 111, 378, 501
SchI GAGTC 2 cut(s) 23, 98
SduI GDGCHC 2 cut(s) 23, 137
SfaNI GCATC 4 cut(s) 154, 271, 697, 730
Sfr303I CCGCGG 1 cut(s) 102
SgrBI CCGCGG 1 cut(s) 102
SinI GGWCC 3 cut(s) 111, 378, 501
SmiMI CAYNNNNRTG 4 cut(s) 162, 324, 330, 726
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 2 cut(s) 329, 418
Sse9I AATT 2 cut(s) 307, 561
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 5 cut(s) 99, 101, 183, 186, 196
SspI AATATT 1 cut(s) 628
SspMI CTAG 2 cut(s) 260, 653
StuI AGGCCT 1 cut(s) 264
TaaI ACNGT 2 cut(s) 611, 675
TaqI TCGA 2 cut(s) 141, 595
TasI AATT 2 cut(s) 307, 561
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 101, 188
TfiI GAWTC 3 cut(s) 9, 236, 446
Tru1I TTAA 2 cut(s) 306, 554
Tru9I TTAA 2 cut(s) 306, 554
TseI GCWGC 4 cut(s) 43, 56, 167, 284
TspDTI ATGAA 1 cut(s) 620
TspGWI ACGGA 1 cut(s) 82
VpaK11BI GGWCC 3 cut(s) 111, 378, 501
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 2 cut(s) 329, 418
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 2 cut(s) 260, 653
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.