MD09G1258900.v1.1

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014371
Physical Location & Seq
Forward (+)
33115153 .. 33125656
10504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1258900.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCAGGATTCCGGTTGGGGCCAGATGACTCGGAGATATCACAGCAGAGCCGAGCAGCACCGCCTGATCTCGTCTCCGACGACGACCGCTCAATCGCCGCCGACTCATGGTCCATCAAGAGTGACTACGGAAGCACTCTCGACGACGACCAGCGCCACGCCGACGCTGCCGAGGACCTTTCTGCTGCTAACCTTCGCGCCCCCTCCGAGTACAGTTCTGACAAGGAGGAACCAGATGCTGAAGGAGTGACATCAATGCTAGGTCTTCAGAGTTACTGGGATGCTGCATATGCCGATGAGTTGGCGAATTTTCGGGAACATGGTCATACCGGTGAAGTTTGGTTCGGAGCTAATGTCATGGAAGTTGTCTCCTCTTGGACCAAAAGCTTATGCATTGATATTTCTCAAGGTCGCACACCAAATCATGATAATAATAAGTCTGATCCTGTGGTACAAGGTAATAATAAGTCTGATCCTGTGGAACAAGGTGATAAGTATCTTTCAGGCTGGAGTGTACTTGACATCGGGACTGGCAATGGATTGCTTCTTCAAGCACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAACTGATTATAGTGAAGGGGCGATTGATCTCGCTCGCAGCCTTGCTGATCGTGATGGATTGCCAAACATTAATTTTTTGGTTGATGATATTCTAGAGACCAAGTTGGACAGGCAGTTCAAACTAGTTGTGGACAAAGGAACCTTGGATGCCATTGGATTGCATCCAGACGGTTCTATCAAAAGGGTAATGTATTGGGATTCCGTTTCAAAATTGGTGGCTCCTGGTGGATTAGTAGTGATCACATCATGTAACAACACAAAGGATGAATTGGTTCAAGAAGTGGAAAGTTTCAATCAAAGAAGTGTCATCCAGGAACACGAAACAAGCAGAGATCTTCCTAAGTTTCAATACCTTAGTCATGTCCGCACATATCCAACATTCGCGTTTGGTGGATCTGTTGGATCGCGCGTTGCCACTGTGGCATTTCTCCGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

342

Amino Acids

37.24

Weight (kDa)

4.5

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 168 - 295 2.7e-17 Methyltransferase domain
Methyltransf_23 PF13489 170 - 296 2.9e-09 Methyltransferase domain
MTS PF05175 171 - 288 7.2e-06 Methyltransferase small domain
Methyltransf_25 PF13649 172 - 246 6.5e-12 Methyltransferase domain
Methyltransf_12 PF08242 173 - 273 5.2e-09 Methyltransferase domain
Methyltransf_11 PF08241 173 - 275 1.1e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AccII CGCG 4 cut(s) 198, 971, 994, 996
AccIII TCCGGA 1 cut(s) 1017
AciI CCGC 4 cut(s) 62, 88, 99, 952
AclWI GGATC 4 cut(s) 437, 467, 988, 997
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 2 cut(s) 251, 261
AfaI GTAC 3 cut(s) 212, 453, 516
AfiI CCNNNNNNNGG 2 cut(s) 108, 567
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 6 cut(s) 551, 706, 795, 863, 880, 935
AhdI GACNNNNNGTC 1 cut(s) 109
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 2 cut(s) 808, 897
AloI GAACNNNNNNTCC 2 cut(s) 686, 718
AluBI AGCT 2 cut(s) 350, 387
AluI AGCT 2 cut(s) 350, 387
Alw26I GTCTC 3 cut(s) 79, 373, 677
AlwI GGATC 4 cut(s) 437, 467, 988, 997
AlwNI CAGNNNCTG 2 cut(s) 239, 590
Aor13HI TCCGGA 1 cut(s) 1017
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 5 cut(s) 56, 167, 185, 284, 624
ApoI RAATTY 1 cut(s) 307
AseI ATTAAT 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 858
AspLEI GCGC 3 cut(s) 156, 200, 996
AspS9I GGNCC 4 cut(s) 20, 111, 175, 378
AsuHPI GGTGA 2 cut(s) 344, 500
AvaII GGWCC 3 cut(s) 111, 175, 378
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 5 cut(s) 68, 154, 172, 271, 636
BccI CCATC 2 cut(s) 122, 635
BciT130I CCWGG 2 cut(s) 810, 899
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 3 cut(s) 79, 373, 677
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 3 cut(s) 260, 680, 710
BfoI RGCGCY 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 1007
BglII AGATCT 1 cut(s) 919
BisI GCNGC 6 cut(s) 57, 99, 168, 186, 285, 625
BlsI GCNGC 6 cut(s) 58, 100, 169, 187, 286, 626
Bme1390I CCNGG 2 cut(s) 810, 899
Bme18I GGWCC 3 cut(s) 111, 175, 378
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 4 cut(s) 20, 111, 175, 378
BmiI GGNNCC 4 cut(s) 21, 231, 727, 807
BmrFI CCNGG 2 cut(s) 810, 899
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 4 cut(s) 226, 271, 724, 757
BmuI ACTGGG 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 2 cut(s) 171, 729
BsaWI WCCGGW 3 cut(s) 12, 329, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 567
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 2 cut(s) 281, 535
Bse3DI GCAATG 1 cut(s) 541
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 810, 899
BseDI CCNNGG 2 cut(s) 171, 729
BseGI GGATG 5 cut(s) 286, 739, 748, 856, 894
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 108, 567
BseMI GCAATG 1 cut(s) 541
BseNI ACTGG 2 cut(s) 281, 535
BseRI GAGGAG 1 cut(s) 361
BseXI GCAGC 5 cut(s) 68, 154, 172, 271, 636
Bsh1236I CGCG 4 cut(s) 198, 971, 994, 996
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 1 cut(s) 22
BshTI ACCGGT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 88
BsiSI CCGG 3 cut(s) 13, 330, 1018
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 108, 567
BsmAI GTCTC 3 cut(s) 79, 373, 677
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 1 cut(s) 677
Bsp13I TCCGGA 1 cut(s) 1017
Bsp143I GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
BspACI CCGC 4 cut(s) 62, 88, 99, 952
BspANI GGCC 1 cut(s) 22
BspEI TCCGGA 1 cut(s) 1017
BspFNI CGCG 4 cut(s) 198, 971, 994, 996
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 21, 231, 727, 807
BspPI GGATC 4 cut(s) 437, 467, 988, 997
BspTNI GGTCTC 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 2 cut(s) 281, 535
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 171, 729
BssMI GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 2 cut(s) 810, 899
Bst4CI ACNGT 3 cut(s) 215, 758, 1006
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 2 cut(s) 927, 941
BstF5I GGATG 5 cut(s) 286, 739, 748, 856, 894
BstFNI CGCG 4 cut(s) 198, 971, 994, 996
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 3 cut(s) 156, 200, 996
BstKTI GATC 9 cut(s) 70, 445, 475, 616, 637, 828, 922, 983, 992
BstMAI GTCTC 3 cut(s) 79, 373, 677
BstMBI GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 167, 290, 1007
BstNI CCWGG 2 cut(s) 810, 899
BstSCI CCNGG 2 cut(s) 808, 897
BstUI CGCG 4 cut(s) 198, 971, 994, 996
BstV1I GCAGC 5 cut(s) 68, 154, 172, 271, 636
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 2 cut(s) 919, 980
BstYI RGATCY 2 cut(s) 919, 980
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 5 cut(s) 286, 739, 748, 856, 894
BtsIMutI CAGTG 1 cut(s) 1002
Cac8I GCNNGC 1 cut(s) 622
CaiI CAGNNNCTG 2 cut(s) 239, 590
CciI TCATGA 1 cut(s) 424
CfoI GCGC 3 cut(s) 156, 200, 996
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 4 cut(s) 20, 111, 175, 378
CseI GACGC 1 cut(s) 173
Csp6I GTAC 3 cut(s) 211, 452, 515
CspAI ACCGGT 1 cut(s) 329
CspCI CAANNNNNGTGG 2 cut(s) 783, 818
CviAII CATG 6 cut(s) 108, 320, 358, 425, 834, 947
CviJI RGCY 7 cut(s) 22, 51, 350, 387, 507, 627, 806
CviKI_1 RGCY 7 cut(s) 22, 51, 350, 387, 507, 627, 806
CviQI GTAC 3 cut(s) 211, 452, 515
DdeI CTNAG 2 cut(s) 927, 941
DpnI GATC 9 cut(s) 69, 444, 474, 615, 636, 827, 921, 982, 991
DpnII GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
DriI GACNNNNNGTC 1 cut(s) 109
Eam1105I GACNNNNNGTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 677
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 111, 175, 378
Eco57I CTGAAG 2 cut(s) 251, 261
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 808, 897
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 729
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 729
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 6 cut(s) 111, 323, 361, 428, 837, 950
FaqI GGGAC 1 cut(s) 541
FatI CATG 6 cut(s) 107, 319, 357, 424, 833, 946
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 825
Fnu4HI GCNGC 6 cut(s) 57, 99, 168, 186, 285, 625
FokI GGATG 5 cut(s) 293, 735, 746, 863, 881
Fsp4HI GCNGC 6 cut(s) 57, 99, 168, 186, 285, 625
FspBI CTAG 3 cut(s) 260, 680, 710
GlaI GCGC 3 cut(s) 155, 199, 995
GluI GCNGC 6 cut(s) 57, 99, 168, 186, 285, 625
GsuI CTGGAG 1 cut(s) 529
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 1 cut(s) 22
HapII CCGG 3 cut(s) 13, 330, 1018
HgaI GACGC 1 cut(s) 173
HhaI GCGC 3 cut(s) 156, 200, 996
Hin1II CATG 6 cut(s) 111, 323, 361, 428, 837, 950
Hin6I GCGC 3 cut(s) 154, 198, 994
HinP1I GCGC 3 cut(s) 154, 198, 994
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 4 cut(s) 9, 29, 104, 785
HpaII CCGG 3 cut(s) 13, 330, 1018
HphI GGTGA 2 cut(s) 344, 500
Hpy166II GTNNAC 2 cut(s) 515, 718
Hpy188I TCNGA 9 cut(s) 34, 79, 208, 220, 270, 347, 442, 472, 577
Hpy8I GTNNAC 2 cut(s) 515, 718
Hpy99I CGWCG 5 cut(s) 83, 86, 146, 149, 167
HpyAV CCTTC 3 cut(s) 203, 236, 596
HpyCH4III ACNGT 3 cut(s) 215, 758, 1006
HpyCH4V TGCA 3 cut(s) 287, 393, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 290, 1007
HpyF3I CTNAG 2 cut(s) 927, 941
Hsp92II CATG 6 cut(s) 111, 323, 361, 428, 837, 950
HspAI GCGC 3 cut(s) 154, 198, 994
Kpn2I TCCGGA 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
LmnI GCTCC 2 cut(s) 347, 811
Lsp1109I GCAGC 5 cut(s) 68, 154, 172, 271, 636
LweI GCATC 4 cut(s) 226, 271, 724, 757
MaeI CTAG 3 cut(s) 260, 680, 710
MaeIII GTNAC 4 cut(s) 122, 247, 272, 836
MalI GATC 9 cut(s) 69, 444, 474, 615, 636, 827, 921, 982, 991
MbiI CCGCTC 1 cut(s) 90
MboI GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
MboII GAAGA 3 cut(s) 257, 539, 914
MflI RGATCY 2 cut(s) 919, 980
MluCI AATT 4 cut(s) 307, 658, 797, 854
MlyI GAGTC 2 cut(s) 23, 98
MmeI TCCRAC 4 cut(s) 102, 672, 967, 986
MnlI CCTC 4 cut(s) 166, 214, 220, 382
Mph1103I ATGCAT 1 cut(s) 395
MroI TCCGGA 1 cut(s) 1017
MroXI GAANNNNTTC 1 cut(s) 858
MseI TTAA 2 cut(s) 581, 657
MslI CAYNNNNRTG 1 cut(s) 330
MspI CCGG 3 cut(s) 13, 330, 1018
MspR9I CCNGG 2 cut(s) 810, 899
MvaI CCWGG 2 cut(s) 810, 899
MvnI CGCG 4 cut(s) 198, 971, 994, 996
MwoI GCNNNNNNNGC 3 cut(s) 167, 290, 1007
NdeI CATATG 1 cut(s) 289
NdeII GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
NlaIII CATG 6 cut(s) 111, 323, 361, 428, 837, 950
NlaIV GGNNCC 4 cut(s) 21, 231, 727, 807
NmeAIII GCCGAG 2 cut(s) 77, 196
NmuCI GTSAC 2 cut(s) 122, 247
NsiI ATGCAT 1 cut(s) 395
PagI TCATGA 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 2 cut(s) 9, 785
PfoI TCCNGGA 1 cut(s) 897
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 6 cut(s) 58, 100, 169, 187, 286, 626
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
PpuMI RGGWCCY 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 808, 897
PspGI CCWGG 2 cut(s) 808, 897
PspN4I GGNNCC 4 cut(s) 21, 231, 727, 807
PspPI GGNCC 4 cut(s) 20, 111, 175, 378
PspPPI RGGWCCY 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 239, 590
PsuI RGATCY 2 cut(s) 919, 980
RsaI GTAC 3 cut(s) 212, 453, 516
RsaNI GTAC 3 cut(s) 211, 452, 515
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 2 cut(s) 581, 657
SatI GCNGC 6 cut(s) 57, 99, 168, 186, 285, 625
Sau3AI GATC 9 cut(s) 67, 442, 472, 613, 634, 825, 919, 980, 989
Sau96I GGNCC 4 cut(s) 20, 111, 175, 378
SchI GAGTC 2 cut(s) 23, 98
ScrFI CCNGG 2 cut(s) 810, 899
SfaNI GCATC 4 cut(s) 226, 271, 724, 757
SinI GGWCC 3 cut(s) 111, 175, 378
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SpeI ACTAGT 1 cut(s) 709
Sse9I AATT 4 cut(s) 307, 658, 797, 854
SsiI CCGC 4 cut(s) 62, 88, 99, 952
SspMI CTAG 3 cut(s) 260, 680, 710
StyD4I CCNGG 2 cut(s) 808, 897
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 3 cut(s) 215, 758, 1006
TaqI TCGA 1 cut(s) 141
TasI AATT 4 cut(s) 307, 658, 797, 854
TatI WGTACW 2 cut(s) 210, 514
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 9, 785
Tru1I TTAA 2 cut(s) 581, 657
Tru9I TTAA 2 cut(s) 581, 657
TscAI CASTG 1 cut(s) 1009
TseFI GTSAC 2 cut(s) 122, 247
TseI GCWGC 5 cut(s) 56, 167, 185, 284, 624
Tsp45I GTSAC 2 cut(s) 122, 247
TspDTI ATGAA 1 cut(s) 867
TspGWI ACGGA 2 cut(s) 144, 778
TspRI CASTG 1 cut(s) 1009
VpaK11BI GGWCC 3 cut(s) 111, 175, 378
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 1 cut(s) 307
XbaI TCTAGA 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 3 cut(s) 260, 680, 710
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.