Rh3CG345800

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0014371
Physical Location & Seq
Reverse (-)
39250965 .. 39255284
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG345800.1

Sequence Viewer

Length: 801 bp
ATGTACAGCTCTGACAAGGAGGAACCGGATTCTGATGGGATGACATCAATGCTAGGCCTTCAGAGTTATTGGGATGCTGCCTATGCAGATGAGCTGGTTAATTTTCGTGAACATGGGCATGCTGGTGAAGTTTGGTTTGGAGCTGATGTCATGGAAGTTGTTGCCTCTTGGACCAAAACATTATGCATTGATGTTTCTCAAGGTCGCATGCCAAATCATGTTGATAATAGCAAGTCTGAATCAGCTGAACAAGGTTACAAGTTTCTTTCAGGTTGGAGTGTACTTGACATTGGGACTGGCAATGGATTGCTTCTTCAAGAACTTTCCAAGCAGGGGTTTTCTGATTTAACAGGAATTGATTATAGTGAAGGGGCGATTGACCTTGCTCGAAACCTTGCTGACCGTGATGGGTTACCCAATATTCATTTTTTGGTTGATGATATCCTAGAGACGAAGTTGGAACGACAGTTTCAGCTTGTTGTAGACAAAGGAACCTTAGATGCCATTGGATTGCATCCAGATGGTGCTATAAAAAGGTTAATATATTGGGATTCAGTTTCGAGATTGATGGCTCCTGGTGGAATATTAGTAATCACATCATGTAACAGCACTAAAGAAGAATTAGTACAAGAAGTGGAAAATTTCAATCAAAGAAGGATTGGTGTGACCCAAGATCAAGAAGGATGCAAAGATACGGCTCCTCAGCTTCAATACCTTAGTCATGTTCGCACATATCCAACATTCATGTTTGGTGGATCTGTAGGATCACGGGTTGCCACTGTGGCATTTCTCCGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

266

Amino Acids

29.44

Weight (kDa)

4.64

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 90 - 216 1.2e-20 Methyltransferase domain
Methyltransf_23 PF13489 90 - 204 2.7e-10 Methyltransferase domain
MTS PF05175 93 - 200 4.6e-06 Methyltransferase small domain
Methyltransf_25 PF13649 94 - 169 4.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 95 - 196 3.9e-10 Methyltransferase domain
Methyltransf_11 PF08241 95 - 198 3.3e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66680
fragaria_vesca FvH4_1g29040 FvH4_1g29040 FvH4_1g29040
malus_domestica MD09G1258900.v1.1 MD17G1253400.v1.1
prunus_persica Prupe.3G150900_v2.0.a1
pyrus_communis pycom09g17370
rosa_chinensis RchiOBHm_Chr3g0493661
rosa_laevigata RLG00000022698
rosa_multiflora Rmu_sc0000568.1_g000014
rosa_roxburghii Rroxscaffold_6G00391390
rosa_rugosa Rorug03G0263300
rosa_samantha Rh3AG312200 Rh3BG348100 Rh3CG345800
rosa_wichuraiana Rw3G027390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 792
AclWI GGATC 2 cut(s) 763, 772
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 3 cut(s) 5, 282, 627
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 3 cut(s) 317, 646, 710
AjnI CCWGG 1 cut(s) 574
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 6 cut(s) 9, 94, 143, 245, 475, 706
AluI AGCT 6 cut(s) 9, 94, 143, 245, 475, 706
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 2 cut(s) 763, 772
Aor13HI TCCGGA 1 cut(s) 792
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 640
AspS9I GGNCC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 171
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 1 cut(s) 64
BccI CCATC 4 cut(s) 29, 401, 515, 562
BceAI ACGGC 1 cut(s) 711
BciT130I CCWGG 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 53, 446
BfmI CTRYAG 1 cut(s) 759
BglI GCCNNNNNGGC 1 cut(s) 782
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 4 cut(s) 24, 493, 573, 699
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 4 cut(s) 64, 490, 523, 674
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 183
BsaWI WCCGGW 2 cut(s) 25, 792
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 307
BseAI TCCGGA 1 cut(s) 792
BseBI CCWGG 1 cut(s) 576
BseGI GGATG 4 cut(s) 45, 79, 514, 689
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 716
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 690
BseXI GCAGC 1 cut(s) 64
BshFI GGCC 1 cut(s) 57
BsiSI CCGG 2 cut(s) 26, 793
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 443
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 307
BsnI GGCC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 792
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 673, 755, 764
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 715
BspEI TCCGGA 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 24, 493, 573, 699
BspPI GGATC 2 cut(s) 763, 772
BsrDI GCAATG 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 301
BssMI GATC 3 cut(s) 673, 755, 764
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 404, 468, 781
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 120, 209
BstDEI CTNAG 3 cut(s) 496, 702, 716
BstEII GGTNACC 1 cut(s) 411
BstF5I GGATG 4 cut(s) 45, 79, 514, 689
BstKTI GATC 3 cut(s) 676, 758, 767
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 3 cut(s) 673, 755, 764
BstMWI GCNNNNNNNGC 2 cut(s) 83, 782
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 2 cut(s) 122, 211
BstPI GGTNACC 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 1 cut(s) 759
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 4 cut(s) 45, 79, 514, 689
BtsIMutI CAGTG 1 cut(s) 777
Cac8I GCNNGC 2 cut(s) 120, 209
Cfr13I GGNCC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 4, 281, 626
CviAII CATG 8 cut(s) 113, 119, 151, 208, 218, 600, 722, 745
CviJI RGCY 9 cut(s) 9, 57, 94, 143, 245, 475, 572, 698, 706
CviKI_1 RGCY 9 cut(s) 9, 57, 94, 143, 245, 475, 572, 698, 706
CviQI GTAC 3 cut(s) 4, 281, 626
DdeI CTNAG 3 cut(s) 496, 702, 716
DpnI GATC 3 cut(s) 675, 757, 766
DpnII GATC 3 cut(s) 673, 755, 764
Eco147I AGGCCT 1 cut(s) 57
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 411
EcoO65I GGTNACC 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 8 cut(s) 116, 122, 154, 211, 221, 603, 725, 748
FaqI GGGAC 1 cut(s) 307
FatI CATG 8 cut(s) 112, 118, 150, 207, 217, 599, 721, 744
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 4 cut(s) 52, 86, 501, 696
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 2 cut(s) 53, 446
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 57
HapII CCGG 2 cut(s) 26, 793
Hin1II CATG 8 cut(s) 116, 122, 154, 211, 221, 603, 725, 748
HinfI GANTC 3 cut(s) 29, 239, 551
HpaII CCGG 2 cut(s) 26, 793
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 3 cut(s) 110, 281, 484
Hpy188I TCNGA 5 cut(s) 13, 34, 63, 238, 343
Hpy188III TCNNGA 6 cut(s) 107, 317, 518, 561, 677, 793
Hpy8I GTNNAC 3 cut(s) 110, 281, 484
HpyAV CCTTC 4 cut(s) 68, 362, 648, 674
HpyCH4III ACNGT 3 cut(s) 404, 468, 781
HpyCH4V TGCA 4 cut(s) 86, 186, 514, 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 782
HpyF3I CTNAG 3 cut(s) 496, 702, 716
Hsp92II CATG 8 cut(s) 116, 122, 154, 211, 221, 603, 725, 748
Kpn2I TCCGGA 1 cut(s) 792
Kzo9I GATC 3 cut(s) 673, 755, 764
LmnI GCTCC 3 cut(s) 140, 577, 703
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 4 cut(s) 64, 490, 523, 674
MaeI CTAG 2 cut(s) 53, 446
MaeIII GTNAC 4 cut(s) 254, 411, 602, 664
MalI GATC 3 cut(s) 675, 757, 766
MboI GATC 3 cut(s) 673, 755, 764
MboII GAAGA 2 cut(s) 305, 629
MflI RGATCY 1 cut(s) 755
MluCI AATT 5 cut(s) 100, 354, 620, 640, 796
MmeI TCCRAC 3 cut(s) 254, 438, 761
MnlI CCTC 3 cut(s) 13, 175, 711
Mph1103I ATGCAT 1 cut(s) 188
MroI TCCGGA 1 cut(s) 792
MseI TTAA 3 cut(s) 99, 347, 539
MslI CAYNNNNRTG 3 cut(s) 117, 123, 519
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 26, 793
MspR9I CCNGG 1 cut(s) 576
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 83, 782
NdeII GATC 3 cut(s) 673, 755, 764
NlaIII CATG 8 cut(s) 116, 122, 154, 211, 221, 603, 725, 748
NlaIV GGNNCC 4 cut(s) 24, 493, 573, 699
NmuCI GTSAC 1 cut(s) 664
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 122, 211
PaeI GCATGC 2 cut(s) 122, 211
PceI AGGCCT 1 cut(s) 57
PfeI GAWTC 3 cut(s) 29, 239, 551
PkrI GCNGC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 574
PspEI GGTNACC 1 cut(s) 411
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 4 cut(s) 24, 493, 573, 699
PspPI GGNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 755
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 3 cut(s) 5, 282, 627
RsaNI GTAC 3 cut(s) 4, 281, 626
RseI CAYNNNNRTG 3 cut(s) 117, 123, 519
SaqAI TTAA 3 cut(s) 99, 347, 539
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 3 cut(s) 673, 755, 764
Sau96I GGNCC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 576
SfaNI GCATC 4 cut(s) 64, 490, 523, 674
SfcI CTRYAG 1 cut(s) 759
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 3 cut(s) 117, 123, 519
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 2 cut(s) 122, 211
Sse9I AATT 5 cut(s) 100, 354, 620, 640, 796
SseBI AGGCCT 1 cut(s) 57
SspI AATATT 2 cut(s) 421, 585
SspMI CTAG 2 cut(s) 53, 446
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 574
TaaI ACNGT 3 cut(s) 404, 468, 781
TaqI TCGA 2 cut(s) 388, 560
TasI AATT 5 cut(s) 100, 354, 620, 640, 796
TatI WGTACW 3 cut(s) 3, 280, 625
TfiI GAWTC 3 cut(s) 29, 239, 551
Tru1I TTAA 3 cut(s) 99, 347, 539
Tru9I TTAA 3 cut(s) 99, 347, 539
TscAI CASTG 1 cut(s) 784
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 2 cut(s) 413, 733
TspRI CASTG 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 171
XapI RAATTY 1 cut(s) 640
XceI RCATGY 2 cut(s) 122, 211
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 53, 446
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.