AT1G66840

Protein PLASTID MOVEMENT IMPAIRED 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007774
Physical Location & Seq
Reverse (-)
24933777 .. 24936512
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66840.1

Sequence Viewer

Length: 1824 bp
ATGGGTGAAAGGAATTTAGATGGAACTGTATCAGTGAAAGCCACCATTAATAAGTACGGACAAAAAGCTACAAGAAGCGTCATTAAGTCTTCGGTTGCTGAGGATTTGCATAAGTCAGGTAGAGAACTTGGTATCTACAGAGAGAGTAGAAGAGTTGCTGAGTCTGCTAAGGCCAAAGCAGAAGTGGAATTGTGTAAGGCGAAGAAGATAGTAAAGGAGCTTACTTTACGGATAGAGGAGTCGAATAGAAGGTTAAAGTCTCGGAGAATCGATATTGAAGCTGTGATGAATGAGTCAAGGATAGATGGGAATGGTGGTTATGTTAGGATTATGAGAGAGTTAGAAGATATGAAGCAAGAATTGAGTAAGCTGAAGCTTGATGTAGTGTATGTTTCGAGAGAGAAAGTTGTGGCGGAGAAAGAAGTAATGGAGTTGGAGTCTAGAATGGAGGAGAATTTGAAGTTGCTTGAGAGTCTCAAGTTAGAAGTTGATGTTGCGAATGAAGAACATGTATTGGTTGAAGTGGCGAAGATCGAGGCTTTGAAGGAGTGTAAAGAGGTTGAAGAGCAGAGGGAGAAGGAAAGGAAGGAAGTGTCTGAGTCATTGCATAAACGAAAGAAAAGAATAAGGGAAATGATTAGAGAGATTGAGAGATCGAAGAACTTTGAGAATGAGTTAGCTGAGACTTTGTTAGATATTGAGATGTTAGAAACTCAGTTAAAGCTTGTCAAGGAAATGGAGAGGAAGGTTCAGAGGAATGAGAGTATGTCGAGATCGAAAAACCGAGCTTTCGAAAGAGGGAAGGATAATTTGTCGGTTTTGAAAGAAGTTACCGAAGCAACAGAAGCGAAGAAGGCAGAACTCGCTTCTATTAACGCGGAGTTATTTTGTCTAGTCAATACAATGGATACATTGAGGAAAGAATTTGACCATGCAAAGAAAGAAACAGCTTGGTTAGATAAGATGATACAAAAGGATGATGTAATGCTTGAGAGACTCAATACGAAGCTGCTTATTGCGAAAGATCAATTAGAAGCAGTATCTAAAGCTGAAGAAAGAATCAGCTATCTTGCTGACAATTTAACTACTTCGTTTGAGAAACTAAAGAGTGATAGAGAAGCTGCAAAGAAAGAGGAGCTTAAGCTTAGAGAAGAAGCAAGGATCATCAACAATGAGATACAGAAAACCGAAACAGGATTTGATGGAAAGGAAAAAGAGTTGCTTTCCAAGTTAGATGAGCTTGAGAAAGCGAAGCACGCAGAGAGTTTAGCTCTAGAGAAGCTTGAAACAATGGTAGAGAAAACAATGGAAACTCGAGAAATGGAATCTCGGCGTAATTCAACTATTACAATATCGAGATTTGAGTACGAGTATTTGAGTGGAAAAGCTTGTCATGCTGAAGAAACTGCAGAGAAGAAAGTGGAAGCAGCAATGGCGTGGGTCGAAGCACTAAAGGCTAGCACTAAGGCGATTATGATAAAGACAGAATCTTTAAAGAGAGTGAGTGGGAAAACAATGTTGGAAGAAGAGAGGGAATCGTTTAGGATGCAGAGGTCGCTATCGATAAAGAGATTGGTCCAAGATGAGATTCAGAAGTTCAAGGGAAATTCAGAAGACAATGGCTTGATCAACTCTCCTAAACCGGTGAGAAAATCGGTTAGGCTGAGCGGGAAATTTGCACCGGTTCAGGGAGGGAAGTCGAGAAGGTACTCATCAGGGAACCGAGCGACTCCAACATTCTTTGTAATCAAGAAGAAGAAGAAAGTTCCAAACATGGTCAAATTTTTTAGCCGGAAGAGGCGTAATTCTTCTTTAGAACAATGA

Protein Analysis

607

Amino Acids

70.24

Weight (kDa)

9.22

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 110 - 500 1.6e-11 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1668
AccII CGCG 1 cut(s) 878
AciI CCGC 3 cut(s) 413, 878, 1668
AclWI GGATC 1 cut(s) 1169
AcsI RAATTY 6 cut(s) 13, 454, 923, 1606, 1673, 1781
AcuI CTGAAG 3 cut(s) 392, 1071, 1419
AfaI GTAC 3 cut(s) 56, 1367, 1709
AfiI CCNNNNNNNGG 1 cut(s) 1688
AflII CTTAAG 1 cut(s) 1139
AflIII ACRYGT 1 cut(s) 508
AgeI ACCGGT 2 cut(s) 1642, 1681
AgsI TTSAA 9 cut(s) 278, 460, 521, 544, 563, 823, 1286, 1341, 1600
AjuI GAANNNNNNNTTGG 4 cut(s) 1502, 1534, 1572, 1604
Alw26I GTCTC 4 cut(s) 264, 479, 677, 988
AlwI GGATC 1 cut(s) 1169
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 1009, 1121, 1427
ApoI RAATTY 6 cut(s) 13, 454, 923, 1606, 1673, 1781
AseI ATTAAT 1 cut(s) 48
AsiGI ACCGGT 2 cut(s) 1642, 1681
AspS9I GGNCC 1 cut(s) 1576
AsuHPI GGTGA 2 cut(s) 17, 1657
AsuII TTCGAA 1 cut(s) 792
AsuNHI GCTAGC 1 cut(s) 1457
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 1576
BbsI GAAGAC 2 cut(s) 81, 1620
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 3 cut(s) 996, 1108, 1439
BccI CCATC 3 cut(s) 14, 299, 1196
BciVI GTATCC 1 cut(s) 901
BclI TGATCA 1 cut(s) 1626
BcoDI GTCTC 4 cut(s) 264, 479, 677, 988
BfaI CTAG 4 cut(s) 441, 893, 1274, 1458
BfmI CTRYAG 2 cut(s) 136, 1407
BfrI CTTAAG 1 cut(s) 1139
BfuI GTATCC 1 cut(s) 901
BisI GCNGC 3 cut(s) 1010, 1122, 1428
BlpI GCTNAGC 1 cut(s) 1664
BlsI GCNGC 3 cut(s) 1011, 1123, 1429
Bme18I GGWCC 1 cut(s) 1576
BmeT110I CYCGRG 1 cut(s) 1314
BmgT120I GGNCC 1 cut(s) 1576
BmiI GGNNCC 1 cut(s) 1721
BmsI GCATC 1 cut(s) 1536
BmtI GCTAGC 1 cut(s) 1461
BpiI GAAGAC 2 cut(s) 81, 1620
BplI GAGNNNNNCTC 2 cut(s) 1255, 1287
Bpu10I CCTNAGC 2 cut(s) 99, 168
Bpu1102I GCTNAGC 1 cut(s) 1664
Bpu14I TTCGAA 1 cut(s) 792
BpuEI CTTGAG 4 cut(s) 461, 488, 1010, 1262
Bsa29I ATCGAT 2 cut(s) 270, 1562
BsaWI WCCGGW 2 cut(s) 1642, 1681
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 1688
Bse118I RCCGGY 2 cut(s) 1642, 1681
Bse3DI GCAATG 2 cut(s) 602, 1437
BseCI ATCGAT 2 cut(s) 270, 1562
BseGI GGATG 2 cut(s) 982, 1551
BseLI CCNNNNNNNGG 1 cut(s) 1688
BseMI GCAATG 2 cut(s) 602, 1437
BseMII CTCAG 6 cut(s) 90, 150, 588, 672, 728, 1655
BseRI GAGGAG 3 cut(s) 251, 464, 1148
BseXI GCAGC 3 cut(s) 996, 1108, 1439
Bsh1236I CGCG 1 cut(s) 878
BshFI GGCC 1 cut(s) 173
BshTI ACCGGT 2 cut(s) 1642, 1681
BshVI ATCGAT 2 cut(s) 270, 1562
BsiHKCI CYCGRG 1 cut(s) 1314
BsiSI CCGG 3 cut(s) 1643, 1682, 1792
BslI CCNNNNNNNGG 1 cut(s) 1688
BsmAI GTCTC 4 cut(s) 264, 479, 677, 988
BsnI GGCC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 1 cut(s) 792
Bsp143I GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
Bsp1720I GCTNAGC 1 cut(s) 1664
BspACI CCGC 3 cut(s) 413, 878, 1668
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 6 cut(s) 91, 151, 589, 673, 727, 1656
BspDI ATCGAT 2 cut(s) 270, 1562
BspFNI CGCG 1 cut(s) 878
BspLI GGNNCC 1 cut(s) 1721
BspMAI CTGCAG 1 cut(s) 1411
BspOI GCTAGC 1 cut(s) 1461
BspPI GGATC 1 cut(s) 1169
BspQI GCTCTTC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 792
BspTI CTTAAG 1 cut(s) 1139
BsrBI CCGCTC 1 cut(s) 1668
BsrDI GCAATG 2 cut(s) 602, 1437
BsrFI RCCGGY 2 cut(s) 1642, 1681
BssAI RCCGGY 2 cut(s) 1642, 1681
BssMI GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 4 cut(s) 145, 558, 1521, 1790
BstAFI CTTAAG 1 cut(s) 1139
BstBI TTCGAA 1 cut(s) 792
BstC8I GCNNGC 2 cut(s) 1257, 1459
BstDEI CTNAG 9 cut(s) 99, 159, 168, 597, 681, 714, 1145, 1464, 1664
BstF5I GGATG 2 cut(s) 982, 1551
BstFNI CGCG 1 cut(s) 878
BstKTI GATC 6 cut(s) 534, 656, 776, 1027, 1164, 1629
BstMAI GTCTC 4 cut(s) 264, 479, 677, 988
BstMBI GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
BstMWI GCNNNNNNNGC 9 cut(s) 164, 845, 854, 863, 1256, 1394, 1433, 1454, 1555
BstNSI RCATGY 1 cut(s) 512
BstSFI CTRYAG 2 cut(s) 136, 1407
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 3 cut(s) 996, 1108, 1439
BstV2I GAAGAC 2 cut(s) 81, 1620
Bsu15I ATCGAT 2 cut(s) 270, 1562
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 1 cut(s) 173
BsuTUI ATCGAT 2 cut(s) 270, 1562
BtsCI GGATG 2 cut(s) 982, 1551
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 1257, 1459
Cfr10I RCCGGY 2 cut(s) 1642, 1681
Cfr13I GGNCC 1 cut(s) 1576
ClaI ATCGAT 2 cut(s) 270, 1562
CseI GACGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 55, 1366, 1708
CspAI ACCGGT 2 cut(s) 1642, 1681
CviAII CATG 4 cut(s) 509, 932, 1394, 1774
CviQI GTAC 3 cut(s) 55, 1366, 1708
DdeI CTNAG 9 cut(s) 99, 159, 168, 597, 681, 714, 1145, 1464, 1664
DpnI GATC 6 cut(s) 533, 655, 775, 1026, 1163, 1628
DpnII GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
DraI TTTAAA 1 cut(s) 1494
Eam1104I CTCTTC 4 cut(s) 145, 558, 1521, 1790
EarI CTCTTC 4 cut(s) 145, 558, 1521, 1790
EciI GGCGGA 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 1576
Eco57I CTGAAG 3 cut(s) 392, 1071, 1419
Eco88I CYCGRG 1 cut(s) 1314
FaeI CATG 4 cut(s) 512, 935, 1397, 1777
FalI AAGNNNNNCTT 4 cut(s) 1122, 1154, 1206, 1238
FatI CATG 4 cut(s) 508, 931, 1393, 1773
FauI CCCGC 1 cut(s) 1661
FbaI TGATCA 1 cut(s) 1626
Fnu4HI GCNGC 3 cut(s) 1010, 1122, 1428
FokI GGATG 2 cut(s) 989, 1558
Fsp4HI GCNGC 3 cut(s) 1010, 1122, 1428
FspBI CTAG 4 cut(s) 441, 893, 1274, 1458
GluI GCNGC 3 cut(s) 1010, 1122, 1428
HaeIII GGCC 1 cut(s) 173
HapII CCGG 3 cut(s) 1643, 1682, 1792
HgaI GACGC 1 cut(s) 67
Hin1II CATG 4 cut(s) 512, 935, 1397, 1777
HindIII AAGCTT 5 cut(s) 374, 722, 1142, 1280, 1386
HpaII CCGG 3 cut(s) 1643, 1682, 1792
HphI GGTGA 2 cut(s) 17, 1657
Hpy188I TCNGA 5 cut(s) 264, 598, 753, 1593, 1612
Hpy188III TCNNGA 8 cut(s) 396, 441, 771, 1274, 1316, 1356, 1701, 1750
HpyAV CCTTC 8 cut(s) 243, 538, 571, 580, 739, 796, 847, 1698
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4V TGCA 7 cut(s) 109, 607, 935, 1124, 1409, 1549, 1679
HpyF10VI GCNNNNNNNGC 9 cut(s) 164, 845, 854, 863, 1256, 1394, 1433, 1454, 1555
HpyF3I CTNAG 9 cut(s) 99, 159, 168, 597, 681, 714, 1145, 1464, 1664
Hsp92II CATG 4 cut(s) 512, 935, 1397, 1777
Ksp22I TGATCA 1 cut(s) 1626
Kzo9I GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 2 cut(s) 217, 1135
LpnPI CCDG 7 cut(s) 102, 1179, 1656, 1673, 1695, 1701, 1805
Lsp1109I GCAGC 3 cut(s) 996, 1108, 1439
LweI GCATC 1 cut(s) 1536
MaeI CTAG 4 cut(s) 441, 893, 1274, 1458
MaeIII GTNAC 1 cut(s) 829
MalI GATC 6 cut(s) 533, 655, 775, 1026, 1163, 1628
MbiI CCGCTC 1 cut(s) 1668
MboI GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
MlyI GAGTC 8 cut(s) 170, 248, 302, 446, 481, 608, 990, 1723
MmeI TCCRAC 3 cut(s) 414, 1500, 1757
MseI TTAA 8 cut(s) 48, 84, 254, 719, 873, 1082, 1140, 1493
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 3 cut(s) 1643, 1682, 1792
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 9 cut(s) 164, 845, 854, 863, 1256, 1394, 1433, 1454, 1555
NdeII GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
NheI GCTAGC 1 cut(s) 1457
NlaIII CATG 4 cut(s) 512, 935, 1397, 1777
NlaIV GGNNCC 1 cut(s) 1721
NmeAIII GCCGAG 1 cut(s) 1309
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 1 cut(s) 792
PaeR7I CTCGAG 1 cut(s) 1314
PciI ACATGT 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 558
PfeI GAWTC 6 cut(s) 267, 1059, 1325, 1487, 1535, 1588
PinAI ACCGGT 2 cut(s) 1642, 1681
PkrI GCNGC 3 cut(s) 1011, 1123, 1429
PleI GAGTC 8 cut(s) 169, 247, 301, 445, 480, 607, 990, 1723
PpsI GAGTC 8 cut(s) 169, 247, 301, 445, 480, 607, 990, 1723
PscI ACATGT 1 cut(s) 508
PshBI ATTAAT 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 1721
PspPI GGNCC 1 cut(s) 1576
PstI CTGCAG 1 cut(s) 1411
RsaI GTAC 3 cut(s) 56, 1367, 1709
RsaNI GTAC 3 cut(s) 55, 1366, 1708
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 8 cut(s) 48, 84, 254, 719, 873, 1082, 1140, 1493
SatI GCNGC 3 cut(s) 1010, 1122, 1428
Sau3AI GATC 6 cut(s) 531, 653, 773, 1024, 1161, 1626
Sau96I GGNCC 1 cut(s) 1576
SchI GAGTC 8 cut(s) 170, 248, 302, 446, 481, 608, 990, 1723
SfaNI GCATC 1 cut(s) 1536
SfcI CTRYAG 2 cut(s) 136, 1407
Sfr274I CTCGAG 1 cut(s) 1314
SfuI TTCGAA 1 cut(s) 792
SinI GGWCC 1 cut(s) 1576
SlaI CTCGAG 1 cut(s) 1314
SmlI CTYRAG 6 cut(s) 467, 476, 989, 1139, 1241, 1314
SmoI CTYRAG 6 cut(s) 467, 476, 989, 1139, 1241, 1314
SsiI CCGC 3 cut(s) 413, 878, 1668
SspMI CTAG 4 cut(s) 441, 893, 1274, 1458
TaaI ACNGT 1 cut(s) 28
TfiI GAWTC 6 cut(s) 267, 1059, 1325, 1487, 1535, 1588
Tru1I TTAA 8 cut(s) 48, 84, 254, 719, 873, 1082, 1140, 1493
Tru9I TTAA 8 cut(s) 48, 84, 254, 719, 873, 1082, 1140, 1493
TscAI CASTG 1 cut(s) 39
TseI GCWGC 3 cut(s) 1009, 1121, 1427
TspDTI ATGAA 3 cut(s) 302, 365, 516
TspGWI ACGGA 2 cut(s) 72, 244
TspRI CASTG 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 1139
VpaK11BI GGWCC 1 cut(s) 1576
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 6 cut(s) 13, 454, 923, 1606, 1673, 1781
XbaI TCTAGA 2 cut(s) 440, 1273
XceI RCATGY 1 cut(s) 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 1314
XspI CTAG 4 cut(s) 441, 893, 1274, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.