Rh2CG292900

Protein PLASTID MOVEMENT IMPAIRED

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007774
Physical Location & Seq
Forward (+)
33222038 .. 33224723
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG292900.1

Sequence Viewer

Length: 1893 bp
ATGAGCAGAAGAAGATTTGGGTCAGTTCGATCTGCTAACAATCTAGTATATGGAGATAGAACACTTGAAGGTAGTCCTTGTCCTTTGCTCAAGAGACAACAGCCCCCATTTGATTCCCCAGAGTCCCCTTCAAGAGCAAGAGAACTCCATATGGCACGGAGGGACATAGGTCGATACAGAGAAACCAGAACTGACACAGACTGGGTCAAAGAACAGGCGCAAATTGAGTTATATGATGCAAAGAAGATAGCCAAAGATCTCTCCTCAGTAATCGAGGAATCAAACTTGATGGCCAGAGGACACACGAGAGAGATTGAAGCACTAAGGAGACCGAGAAGGCGTGGAGATAATCGAGTATTATTGCCTGTTGGGAATGATCATGTGGAGAATAACAAAAATTATGAACAAGTCATGAGAGAAGTGCAGCTTTTGAAACAGGAACTGAGCATGCTCAAGATCGATATGGCTAATGTTTTGGAAGAGAAATCACAGGCAGAGAAGCAAGCAGAAGCCGCAAATGAAAAGATGATGTTTTATACAAGCTCGGCGGAAGCTATCAGGAAGGAGATTGAGGAAGCTGATGAAGAGCAAGTGCTAGTTGAGTTGGCTCAGATTGAAGCCTTGAAAGAATTTGGAGATATTGAAGCTCAGAGACAACAAGAAGCCAATCAACTCTCATCTGCAGTGGAGGAAACCAGGAATAAAGTGAAAGATATTACTGAAGAGATTGATCACTCAAAACAAGTTGAAACCAAATTGTCTGTCACATTGTCTAATGTCGATGCGTTACAGAATGAACTACAGCTAGTTAAAGGATTGGACAAAAGTAGTAGTCAAATGACTGATAGCTTTCAGCTTTCGGAAACCAGTCTCCAAAGAGCAGAGGACTCGGAAGCTTCACCCTTGTTACACTCAATCACAGAAGAACTAGAGGCGGCAAAGAAAGAACTGGCTGCAGTAAAGGAAGAAGGATTCCAGTACATGGCCTCCATGGATGTTATAAGAAACGAGATGAAGCATATCACAGATCAGACAGCTCGGATAAAGAAGGCAGAGGATAAAGCAGATTTAACTGTTCAAACTCTCAATTCAAAGTTTCTCAGAGCCAAAGCCAAGTTGGAAGCTTTATGTGCATCTGAAGAAAAAGCAAAATCAATTCAATCCAGTCTCTCACTCACTCTTGAACAGCTTAAGACAGATGCTGATGCCGCAAACAAAGAGAAGGAGAATGCCAGTGAAGAAGCTGCAACCATTAAGTCGGAAATACTCAAGATTGAATCGGATATAGACATAGCTGAAGAAAAATTTGAGGCTGCAATGCAAGAGCTTGAAGCAGTAAAATCTTCAGAGGCTGTAGCTCTTGAAAACCTCAAAACACTTATTGAGAATACCATGAGAGCTAGAGCTTCTGCAGCTCAGAATAGTTCTTCAATTACCATATCCAAGTTTGAATATGAGTACTTGACTGGACGTGCAGTTGGGGCTGAAGAAATTGCTGATAAAAAGGTAGCAGCTGCTCAGGCTTGGGTTGAAGCTATAAAGGCCAGTGAGAAGGAAATATTGATAAAGATAGACTTAGCTGAGAAAGAGCTGAAAGAAACGAGGGTGGAGGAGGTACAGCAAGAGGCCTCTAGACCTGAAAGACAACCTTCTGGACAGAGGATGCTGGAGGGACAAGAATTACAAATTTGGAGACAAAAGAGGGACAGAAATGCAGCATCTGGAAACCGGCGAACTAATGGAAATTTAACTCCATCAAGGCGAGCCAAGTTCCAAAAATCTGCCTCTCCTGGAGCTCGGCATATGAATAATTTCCCCATTCAGAAAAAGACGAAGGTAATGCCTAATCTGGGCAAGTTTTTTGGCGGTAAGAGAATTGCAAAAGATGTGTGA

Protein Analysis

630

Amino Acids

71.12

Weight (kDa)

5.67

Isoelectric Point (pI)

58.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 47 - 569 6.1e-26 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 982
AciI CCGC 5 cut(s) 513, 548, 935, 1209, 1866
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 4 cut(s) 629, 1304, 1686, 1744
AcuI CTGAAG 5 cut(s) 741, 1158, 1317, 1329, 1506
AfaI GTAC 3 cut(s) 980, 1460, 1617
AfiI CCNNNNNNNGG 2 cut(s) 982, 1850
AflII CTTAAG 1 cut(s) 1190
AjiI CACGTC 1 cut(s) 1472
AjnI CCWGG 2 cut(s) 695, 1789
Alw21I GWGCWC 1 cut(s) 1798
Alw26I GTCTC 6 cut(s) 88, 322, 646, 875, 1172, 1687
AlwNI CAGNNNCTG 4 cut(s) 442, 1202, 1352, 1721
AoxI GGCC 4 cut(s) 291, 984, 1542, 1626
ApeKI GCWGC 8 cut(s) 424, 953, 1244, 1313, 1412, 1511, 1514, 1715
ApoI RAATTY 4 cut(s) 629, 1304, 1686, 1744
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 1 cut(s) 1811
AspLEI GCGC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 891
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 1 cut(s) 1798
BauI CACGAG 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 1798
BbvI GCAGC 8 cut(s) 436, 940, 1231, 1300, 1424, 1501, 1523, 1727
BccI CCATC 2 cut(s) 283, 1762
BcgI CGANNNNNNTGC 4 cut(s) 870, 904, 1822, 1856
BciT130I CCWGG 2 cut(s) 697, 1791
BclI TGATCA 2 cut(s) 376, 730
BcoDI GTCTC 6 cut(s) 88, 322, 646, 875, 1172, 1687
BfaI CTAG 6 cut(s) 44, 596, 806, 929, 1401, 1632
BfmI CTRYAG 5 cut(s) 681, 800, 954, 1353, 1410
BfrI CTTAAG 1 cut(s) 1190
BglII AGATCT 1 cut(s) 256
BmcAI AGTACT 1 cut(s) 1460
Bme1390I CCNGG 2 cut(s) 697, 1791
BmgBI CACGTC 1 cut(s) 1472
BmrFI CCNGG 2 cut(s) 697, 1791
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 7 cut(s) 226, 772, 1142, 1189, 1195, 1653, 1727
BmuI ACTGGG 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 168
BpmI CTGGAG 2 cut(s) 1688, 1812
Bpu10I CCTNAGC 1 cut(s) 1518
BpuEI CTTGAG 3 cut(s) 74, 437, 1253
Bsa29I ATCGAT 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 1 cut(s) 990
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 982, 1850
Bse118I RCCGGY 1 cut(s) 1728
Bse1I ACTGG 8 cut(s) 206, 867, 954, 976, 1164, 1233, 1471, 1545
Bse3DI GCAATG 1 cut(s) 1323
BseBI CCWGG 2 cut(s) 697, 1791
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 990
BseGI GGATG 2 cut(s) 1000, 1668
BseLI CCNNNNNNNGG 2 cut(s) 982, 1850
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 8 cut(s) 279, 434, 623, 662, 1114, 1430, 1532, 1572
BseNI ACTGG 8 cut(s) 206, 867, 954, 976, 1164, 1233, 1471, 1545
BseRI GAGGAG 2 cut(s) 253, 1625
BseXI GCAGC 8 cut(s) 436, 940, 1231, 1300, 1424, 1501, 1523, 1727
BsgI GTGCAG 2 cut(s) 443, 1494
BshFI GGCC 4 cut(s) 293, 986, 1544, 1628
BshVI ATCGAT 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 1798
BsiSI CCGG 1 cut(s) 1729
BslFI GGGAC 4 cut(s) 109, 176, 1686, 1718
BslI CCNNNNNNNGG 2 cut(s) 982, 1850
BsmAI GTCTC 6 cut(s) 88, 322, 646, 875, 1172, 1687
BsmFI GGGAC 4 cut(s) 109, 176, 1686, 1718
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 4 cut(s) 293, 986, 1544, 1628
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1798
Bsp143I GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
Bsp19I CCATGG 1 cut(s) 990
BspACI CCGC 5 cut(s) 513, 548, 935, 1209, 1866
BspANI GGCC 4 cut(s) 293, 986, 1544, 1628
BspCNI CTCAG 8 cut(s) 278, 435, 622, 661, 1113, 1429, 1531, 1573
BspDI ATCGAT 1 cut(s) 459
BspHI TCATGA 1 cut(s) 411
BspMAI CTGCAG 3 cut(s) 685, 958, 1414
BspQI GCTCTTC 1 cut(s) 579
BspTI CTTAAG 1 cut(s) 1190
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 1728
BsrI ACTGG 8 cut(s) 206, 867, 954, 976, 1164, 1233, 1471, 1545
BssAI RCCGGY 1 cut(s) 1728
BssECI CCNNGG 1 cut(s) 990
BssMI GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
BssSI CACGAG 1 cut(s) 304
BssT1I CCWWGG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 304
Bst2UI CCWGG 2 cut(s) 697, 1791
Bst4CI ACNGT 1 cut(s) 1075
Bst6I CTCTTC 3 cut(s) 474, 579, 717
BstAFI CTTAAG 1 cut(s) 1190
BstC8I GCNNGC 3 cut(s) 449, 504, 1764
BstDSI CCRYGG 1 cut(s) 990
BstF5I GGATG 2 cut(s) 1000, 1668
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 6 cut(s) 32, 259, 379, 459, 733, 1030
BstMAI GTCTC 6 cut(s) 88, 322, 646, 875, 1172, 1687
BstMBI GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
BstMWI GCNNNNNNNGC 7 cut(s) 512, 1130, 1208, 1412, 1481, 1520, 1541
BstNI CCWGG 2 cut(s) 697, 1791
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 695, 1789
BstSFI CTRYAG 5 cut(s) 681, 800, 954, 1353, 1410
BstV1I GCAGC 8 cut(s) 436, 940, 1231, 1300, 1424, 1501, 1523, 1727
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 4 cut(s) 293, 986, 1544, 1628
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 990
BtrI CACGTC 1 cut(s) 1472
BtsCI GGATG 2 cut(s) 1000, 1668
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 3 cut(s) 690, 1240, 1552
Cac8I GCNNGC 3 cut(s) 449, 504, 1764
CaiI CAGNNNCTG 4 cut(s) 442, 1202, 1352, 1721
CciI TCATGA 1 cut(s) 411
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 1728
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 3 cut(s) 979, 1459, 1616
CviAII CATG 6 cut(s) 380, 412, 448, 982, 991, 1393
CviQI GTAC 3 cut(s) 979, 1459, 1616
DpnI GATC 6 cut(s) 31, 258, 378, 458, 732, 1029
DpnII GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 3 cut(s) 474, 579, 717
EarI CTCTTC 3 cut(s) 474, 579, 717
EciI GGCGGA 1 cut(s) 563
Ecl136II GAGCTC 1 cut(s) 1796
Eco130I CCWWGG 1 cut(s) 990
Eco147I AGGCCT 1 cut(s) 1628
Eco24I GRGCYC 1 cut(s) 1798
Eco31I GGTCTC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 1796
Eco57I CTGAAG 5 cut(s) 741, 1158, 1317, 1329, 1506
EcoICRI GAGCTC 1 cut(s) 1796
EcoRII CCWGG 2 cut(s) 695, 1789
EcoT14I CCWWGG 1 cut(s) 990
EcoT38I GRGCYC 1 cut(s) 1798
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 6 cut(s) 383, 415, 451, 985, 994, 1396
FalI AAGNNNNNCTT 6 cut(s) 411, 443, 1559, 1591, 1633, 1665
FaqI GGGAC 4 cut(s) 109, 176, 1686, 1718
FatI CATG 6 cut(s) 379, 411, 447, 981, 990, 1392
FauNDI CATATG 2 cut(s) 150, 1803
FbaI TGATCA 2 cut(s) 376, 730
FokI GGATG 2 cut(s) 1007, 1675
FriOI GRGCYC 1 cut(s) 1798
FspBI CTAG 6 cut(s) 44, 596, 806, 929, 1401, 1632
GlaI GCGC 1 cut(s) 219
GsuI CTGGAG 2 cut(s) 1688, 1812
HaeIII GGCC 4 cut(s) 293, 986, 1544, 1628
HapII CCGG 1 cut(s) 1729
HhaI GCGC 1 cut(s) 220
Hin1II CATG 6 cut(s) 383, 415, 451, 985, 994, 1396
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HindIII AAGCTT 2 cut(s) 894, 1122
HinfI GANTC 6 cut(s) 113, 122, 278, 887, 972, 1277
HpaII CCGG 1 cut(s) 1729
HphI GGTGA 1 cut(s) 891
HpyCH4III ACNGT 1 cut(s) 1075
HpyCH4IV ACGT 1 cut(s) 1471
HpyF10VI GCNNNNNNNGC 7 cut(s) 512, 1130, 1208, 1412, 1481, 1520, 1541
HpySE526I ACGT 1 cut(s) 1471
Hsp92II CATG 6 cut(s) 383, 415, 451, 985, 994, 1396
HspAI GCGC 1 cut(s) 218
Ksp22I TGATCA 2 cut(s) 376, 730
Kzo9I GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 1 cut(s) 1793
Lsp1109I GCAGC 8 cut(s) 436, 940, 1231, 1300, 1424, 1501, 1523, 1727
LweI GCATC 7 cut(s) 226, 772, 1142, 1189, 1195, 1653, 1727
MaeI CTAG 6 cut(s) 44, 596, 806, 929, 1401, 1632
MaeII ACGT 1 cut(s) 1471
MaeIII GTNAC 3 cut(s) 763, 786, 906
MalI GATC 6 cut(s) 31, 258, 378, 458, 732, 1029
MboI GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 1798
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 2 cut(s) 131, 881
MmeI TCCRAC 2 cut(s) 1098, 1239
Mox20I TGGCCA 1 cut(s) 293
MroXI GAANNNNTTC 1 cut(s) 1811
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 810, 1070, 1191, 1254, 1748
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 1514
MspCI CTTAAG 1 cut(s) 1190
MspI CCGG 1 cut(s) 1729
MspR9I CCNGG 2 cut(s) 697, 1791
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 2 cut(s) 697, 1791
MwoI GCNNNNNNNGC 7 cut(s) 512, 1130, 1208, 1412, 1481, 1520, 1541
NcoI CCATGG 1 cut(s) 990
NdeI CATATG 2 cut(s) 150, 1803
NdeII GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
NlaIII CATG 6 cut(s) 383, 415, 451, 985, 994, 1396
NmeAIII GCCGAG 2 cut(s) 524, 1777
NmuCI GTSAC 1 cut(s) 763
NspI RCATGY 1 cut(s) 451
PaeI GCATGC 1 cut(s) 451
PagI TCATGA 1 cut(s) 411
PceI AGGCCT 1 cut(s) 1628
PciSI GCTCTTC 1 cut(s) 579
PctI GAATGC 1 cut(s) 1234
PdmI GAANNNNTTC 1 cut(s) 1811
PfeI GAWTC 4 cut(s) 113, 278, 972, 1277
PflFI GACNNNGTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 982
PfoI TCCNGGA 1 cut(s) 1789
PleI GAGTC 2 cut(s) 130, 881
PpsI GAGTC 2 cut(s) 130, 881
PshAI GACNNNNGTC 1 cut(s) 168
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 1798
Psp6I CCWGG 2 cut(s) 695, 1789
PspGI CCWGG 2 cut(s) 695, 1789
PstI CTGCAG 3 cut(s) 685, 958, 1414
PstNI CAGNNNCTG 4 cut(s) 442, 1202, 1352, 1721
PsuI RGATCY 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 203
PvuII CAGCTG 1 cut(s) 1514
RsaI GTAC 3 cut(s) 980, 1460, 1617
RsaNI GTAC 3 cut(s) 979, 1459, 1616
SacI GAGCTC 1 cut(s) 1798
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 5 cut(s) 810, 1070, 1191, 1254, 1748
Sau3AI GATC 6 cut(s) 29, 256, 376, 456, 730, 1027
ScaI AGTACT 1 cut(s) 1460
SchI GAGTC 2 cut(s) 131, 881
ScrFI CCNGG 2 cut(s) 697, 1791
SduI GDGCHC 1 cut(s) 1798
SfaNI GCATC 7 cut(s) 226, 772, 1142, 1189, 1195, 1653, 1727
SfcI CTRYAG 5 cut(s) 681, 800, 954, 1353, 1410
SmlI CTYRAG 4 cut(s) 89, 452, 1190, 1268
SmoI CTYRAG 4 cut(s) 89, 452, 1190, 1268
SphI GCATGC 1 cut(s) 451
SseBI AGGCCT 1 cut(s) 1628
SsiI CCGC 5 cut(s) 513, 548, 935, 1209, 1866
SspI AATATT 1 cut(s) 1560
SspMI CTAG 6 cut(s) 44, 596, 806, 929, 1401, 1632
SstI GAGCTC 1 cut(s) 1798
StuI AGGCCT 1 cut(s) 1628
StyD4I CCNGG 2 cut(s) 695, 1789
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 1 cut(s) 1075
TaiI ACGT 1 cut(s) 1474
TaqI TCGA 6 cut(s) 28, 172, 273, 352, 459, 780
TaqII GACCGA 1 cut(s) 346
TatI WGTACW 2 cut(s) 978, 1458
TauI GCSGC 3 cut(s) 515, 938, 1211
TfiI GAWTC 4 cut(s) 113, 278, 972, 1277
Tru1I TTAA 5 cut(s) 810, 1070, 1191, 1254, 1748
Tru9I TTAA 5 cut(s) 810, 1070, 1191, 1254, 1748
TscAI CASTG 3 cut(s) 690, 1240, 1552
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 8 cut(s) 424, 953, 1244, 1313, 1412, 1511, 1514, 1715
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 6 cut(s) 417, 534, 597, 810, 1028, 1820
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 3 cut(s) 690, 1240, 1552
Tth111I GACNNNGTC 1 cut(s) 203
Van91I CCANNNNNTGG 1 cut(s) 982
Vha464I CTTAAG 1 cut(s) 1190
XapI RAATTY 4 cut(s) 629, 1304, 1686, 1744
XbaI TCTAGA 1 cut(s) 1631
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XmnI GAANNNNTTC 1 cut(s) 1811
XspI CTAG 6 cut(s) 44, 596, 806, 929, 1401, 1632
ZrmI AGTACT 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.