RLG00000018771

Protein PLASTID MOVEMENT IMPAIRED

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007774
Physical Location & Seq
Forward (+)
35721788 .. 35723530
1743 bp
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UTR
Exon/CDS
Intron
RLM00000018771

Sequence Viewer

Length: 1743 bp
ATGGCACGGAGGGACATAGGTCGATACAGAGAAATCAGAGCTGACACAGACTCGGTCAAAGAACAGGCGCAAATTGAGTTATATGATGCAAAGAAGATAGCCAAAGATCTCTCCTCAGTAATTGAGGAATCAAATTTGATGGCCAGAGGACACACGAGAGAGATTGAAGCACTAAGGAGACCGAGAAGGCGTGGAGATAATCGAGTATTATTGCCTGTTGGGAATGATCATGTGGAGAATAACAAAAATTATGAACAAGTCATGAGAGAATTGGAGCTTTTGAAACAGGAACTGAGCATGCTCAAGATCGATATGGCTAATGTTCTGGAAGAGAAATCGCAGGCAGAGAAGCAAGCAGAAGCCGCAAATGAAAAGATGATGTTTTATACAAGCTCGGCGGAAGCTATCAGGAAGGAGATTGAGGAAGCTGATGAAGAGCAAGTGCTAGTTGAGTTGGCTCAGATTGAAGCCTTGAAAGAATTTGGAGATATTGAAGCTCAGAGACAACAAGAAGCCAATCAACTCTCATCTGCAGTGGAGGAAACCAGGAAAAAAGTGAAAGATATTACTGAAGAGATTGATCACTCAAAACAAGTTGAAACCAAATTGTCTGTCACATTGTCTAATGTTGATGCGTTACGGAATGAACTACAGCTAGTTAAAGGAATGGACAAAAGTAGTAGTCAAATGACTGATAGCTTTCAGCTTTCGGAAACCAGTCTCCAAAGAACAGAGGACTCGGAAGCTTCACCCTTGTTACACTCAATCACAGAAGAACTAGAGGCGGCAAAGAAAGAACTGGCTGCAGTAAAGGAAGAAGGATTCCAGTACATGGCCTCCATGGATGTTATAAGAAACGAGATGAAGCATATCACAGATCAGACAACTCGGATAAAGAAGGCAGAGGATAAAGCAGATTTTACTGTTCAAACTCTCAATTCAAAGCTTCTCAGAGCCAAAGCCAAGTTGGAAGCTTTATGTGCATCTGAAGAAAAAGCAAAATCAATTCAATCCAGTCTCTCACTCACTCTTGAACAGCTTAAGACAGATGCTGATGCCGCAAACAAAGAGAAGGAGCATGCCAGTGAAGAAGCTGCAACCATTAAGTCAGAAATACTCAAGATTGAATCGGATATAGACATGGCTGAGGAAAAATTTGAGGCTGCAATGCAAGAGCTTGAAGCAGTAAAATCTTCAGAGGCTGTAGCTCTTGAAAACCTCAAAACGCTTATTGAGAATACCATGAGAGCTAGAGCTTCTGCAGCTCAGAATAGTTCTTCAATTACCATATCCAAGTTTGAATATGAGTACTTGACTGGACGTGCAGTTGGGGCTGAAGAAATTGCTGATAAAAAGGTAGCAGCTGCTCAGGCTTGGGTTGAAGCTATAAAGGCCAGTGAGAAGGAAATATTGATAAAGATAGACTTAGCTGAAAAAGAGCTGAAAGAAACGAGGGTGGAGGAGGTCCAGCAAGAGGCCTCTAGACCTGAAAGACAACCTTCTGGACAGAGGATGCTTGAGGGACAAGAATTACAAATTTGGAGACAAAAGAGGGACAGAAATGCAGCATCTGGAAACCGGCGAGCTTATGGAAATTTAACTCCATCAAGGCGAGCCAAGTTCCGAAAATCTGCCTCTCCTGGAGCTCGGCATATGAATAATTTCCCCATTCAGAAAAAGACGAAGGTAATGCCTAATCTGGGCAAGTTTTTTGGGGGTAAGAGAATTGCAAAAGATGTGTGA

Protein Analysis

581

Amino Acids

65.55

Weight (kDa)

5.54

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 3 - 519 7e-26 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 851
AccB7I CCANNNNNTGG 1 cut(s) 832
AciI CCGC 4 cut(s) 363, 398, 785, 1059
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 5 cut(s) 133, 479, 1154, 1536, 1594
AcuI CTGAAG 4 cut(s) 591, 1008, 1179, 1356
AfaI GTAC 2 cut(s) 830, 1310
AfiI CCNNNNNNNGG 3 cut(s) 832, 1474, 1700
AflII CTTAAG 1 cut(s) 1040
AjiI CACGTC 1 cut(s) 1322
AjnI CCWGG 2 cut(s) 545, 1639
Alw21I GWGCWC 1 cut(s) 1648
Alw26I GTCTC 5 cut(s) 172, 496, 725, 1022, 1537
AlwNI CAGNNNCTG 4 cut(s) 292, 1052, 1202, 1571
AoxI GGCC 4 cut(s) 141, 834, 1392, 1476
ApeKI GCWGC 7 cut(s) 803, 1094, 1163, 1262, 1361, 1364, 1565
ApoI RAATTY 5 cut(s) 133, 479, 1154, 1536, 1594
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
Asp700I GAANNNNTTC 1 cut(s) 1661
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 1465
AsuHPI GGTGA 1 cut(s) 741
AvaII GGWCC 1 cut(s) 1465
BalI TGGCCA 1 cut(s) 143
BanII GRGCYC 1 cut(s) 1648
BauI CACGAG 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 1648
BbvCI CCTCAGC 1 cut(s) 1146
BbvI GCAGC 7 cut(s) 790, 1081, 1150, 1274, 1351, 1373, 1577
BccI CCATC 2 cut(s) 133, 1612
BcgI CGANNNNNNTGC 2 cut(s) 1672, 1706
BciT130I CCWGG 2 cut(s) 547, 1641
BclI TGATCA 2 cut(s) 226, 580
BcoDI GTCTC 5 cut(s) 172, 496, 725, 1022, 1537
BfaI CTAG 5 cut(s) 446, 656, 779, 1251, 1482
BfmI CTRYAG 5 cut(s) 531, 650, 804, 1203, 1260
BfrI CTTAAG 1 cut(s) 1040
BglII AGATCT 1 cut(s) 106
BmcAI AGTACT 1 cut(s) 1310
Bme1390I CCNGG 2 cut(s) 547, 1641
Bme18I GGWCC 1 cut(s) 1465
BmgBI CACGTC 1 cut(s) 1322
BmgT120I GGNCC 1 cut(s) 1465
BmrFI CCNGG 2 cut(s) 547, 1641
BmsI GCATC 7 cut(s) 76, 622, 992, 1039, 1045, 1503, 1577
BoxI GACNNNNGTC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 1662
Bpu10I CCTNAGC 2 cut(s) 1146, 1368
BpuEI CTTGAG 3 cut(s) 287, 1103, 1538
Bsa29I ATCGAT 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 840
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 3 cut(s) 832, 1474, 1700
Bse118I RCCGGY 1 cut(s) 1578
Bse1I ACTGG 7 cut(s) 717, 804, 826, 1014, 1083, 1321, 1395
Bse3DI GCAATG 1 cut(s) 1173
BseBI CCWGG 2 cut(s) 547, 1641
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 2 cut(s) 850, 1518
BseLI CCNNNNNNNGG 3 cut(s) 832, 1474, 1700
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 8 cut(s) 129, 284, 473, 512, 964, 1137, 1280, 1382
BseNI ACTGG 7 cut(s) 717, 804, 826, 1014, 1083, 1321, 1395
BseRI GAGGAG 2 cut(s) 103, 1475
BseXI GCAGC 7 cut(s) 790, 1081, 1150, 1274, 1351, 1373, 1577
BsgI GTGCAG 1 cut(s) 1344
BshFI GGCC 4 cut(s) 143, 836, 1394, 1478
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 1648
BsiSI CCGG 1 cut(s) 1579
BslFI GGGAC 3 cut(s) 26, 1536, 1568
BslI CCNNNNNNNGG 3 cut(s) 832, 1474, 1700
BsmAI GTCTC 5 cut(s) 172, 496, 725, 1022, 1537
BsmFI GGGAC 3 cut(s) 26, 1536, 1568
BsnI GGCC 4 cut(s) 143, 836, 1394, 1478
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 1648
Bsp143I GATC 5 cut(s) 106, 226, 306, 580, 877
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 4 cut(s) 363, 398, 785, 1059
BspANI GGCC 4 cut(s) 143, 836, 1394, 1478
BspCNI CTCAG 8 cut(s) 128, 285, 472, 511, 963, 1138, 1279, 1381
BspDI ATCGAT 1 cut(s) 309
BspHI TCATGA 1 cut(s) 261
BspMAI CTGCAG 3 cut(s) 535, 808, 1264
BspQI GCTCTTC 1 cut(s) 429
BspTI CTTAAG 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 1173
BsrFI RCCGGY 1 cut(s) 1578
BsrI ACTGG 7 cut(s) 717, 804, 826, 1014, 1083, 1321, 1395
BssAI RCCGGY 1 cut(s) 1578
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 5 cut(s) 106, 226, 306, 580, 877
BssSI CACGAG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 840
Bst2BI CACGAG 1 cut(s) 154
Bst2UI CCWGG 2 cut(s) 547, 1641
Bst4CI ACNGT 1 cut(s) 925
Bst6I CTCTTC 3 cut(s) 324, 429, 567
BstAFI CTTAAG 1 cut(s) 1040
BstC8I GCNNGC 6 cut(s) 299, 342, 354, 1080, 1584, 1614
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 2 cut(s) 850, 1518
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 5 cut(s) 109, 229, 309, 583, 880
BstMAI GTCTC 5 cut(s) 172, 496, 725, 1022, 1537
BstMBI GATC 5 cut(s) 106, 226, 306, 580, 877
BstMWI GCNNNNNNNGC 7 cut(s) 362, 980, 1058, 1262, 1331, 1370, 1391
BstNI CCWGG 2 cut(s) 547, 1641
BstNSI RCATGY 2 cut(s) 301, 1082
BstPAI GACNNNNGTC 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 545, 1639
BstSFI CTRYAG 5 cut(s) 531, 650, 804, 1203, 1260
BstV1I GCAGC 7 cut(s) 790, 1081, 1150, 1274, 1351, 1373, 1577
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 4 cut(s) 143, 836, 1394, 1478
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 840
BtrI CACGTC 1 cut(s) 1322
BtsCI GGATG 2 cut(s) 850, 1518
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 3 cut(s) 540, 1090, 1402
Cac8I GCNNGC 6 cut(s) 299, 342, 354, 1080, 1584, 1614
CaiI CAGNNNCTG 4 cut(s) 292, 1052, 1202, 1571
CciI TCATGA 1 cut(s) 261
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 1 cut(s) 1578
Cfr13I GGNCC 1 cut(s) 1465
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 2 cut(s) 829, 1309
CviAII CATG 8 cut(s) 230, 262, 298, 832, 841, 1079, 1141, 1243
CviQI GTAC 2 cut(s) 829, 1309
DpnI GATC 5 cut(s) 108, 228, 308, 582, 879
DpnII GATC 5 cut(s) 106, 226, 306, 580, 877
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 3 cut(s) 324, 429, 567
EarI CTCTTC 3 cut(s) 324, 429, 567
EciI GGCGGA 1 cut(s) 413
Ecl136II GAGCTC 1 cut(s) 1646
Eco130I CCWWGG 1 cut(s) 840
Eco147I AGGCCT 1 cut(s) 1478
Eco24I GRGCYC 1 cut(s) 1648
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 1465
Eco53kI GAGCTC 1 cut(s) 1646
Eco57I CTGAAG 4 cut(s) 591, 1008, 1179, 1356
EcoICRI GAGCTC 1 cut(s) 1646
EcoRII CCWGG 2 cut(s) 545, 1639
EcoT14I CCWWGG 1 cut(s) 840
EcoT38I GRGCYC 1 cut(s) 1648
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 8 cut(s) 233, 265, 301, 835, 844, 1082, 1144, 1246
FalI AAGNNNNNCTT 4 cut(s) 1409, 1441, 1483, 1515
FaqI GGGAC 3 cut(s) 26, 1536, 1568
FatI CATG 8 cut(s) 229, 261, 297, 831, 840, 1078, 1140, 1242
FauNDI CATATG 1 cut(s) 1653
FbaI TGATCA 2 cut(s) 226, 580
FokI GGATG 2 cut(s) 857, 1525
FriOI GRGCYC 1 cut(s) 1648
FspBI CTAG 5 cut(s) 446, 656, 779, 1251, 1482
GlaI GCGC 1 cut(s) 69
GsuI CTGGAG 1 cut(s) 1662
HaeIII GGCC 4 cut(s) 143, 836, 1394, 1478
HapII CCGG 1 cut(s) 1579
HhaI GCGC 1 cut(s) 70
Hin1II CATG 8 cut(s) 233, 265, 301, 835, 844, 1082, 1144, 1246
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 3 cut(s) 744, 944, 972
HinfI GANTC 5 cut(s) 50, 128, 737, 822, 1127
HpaII CCGG 1 cut(s) 1579
HphI GGTGA 1 cut(s) 741
HpyAV CCTTC 8 cut(s) 180, 406, 812, 892, 1066, 1396, 1509, 1678
HpyCH4III ACNGT 1 cut(s) 925
HpyCH4IV ACGT 1 cut(s) 1321
HpyF10VI GCNNNNNNNGC 7 cut(s) 362, 980, 1058, 1262, 1331, 1370, 1391
HpySE526I ACGT 1 cut(s) 1321
Hsp92II CATG 8 cut(s) 233, 265, 301, 835, 844, 1082, 1144, 1246
HspAI GCGC 1 cut(s) 68
Ksp22I TGATCA 2 cut(s) 226, 580
Kzo9I GATC 5 cut(s) 106, 226, 306, 580, 877
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 274, 1075, 1643
Lsp1109I GCAGC 7 cut(s) 790, 1081, 1150, 1274, 1351, 1373, 1577
LweI GCATC 7 cut(s) 76, 622, 992, 1039, 1045, 1503, 1577
MaeI CTAG 5 cut(s) 446, 656, 779, 1251, 1482
MaeII ACGT 1 cut(s) 1321
MaeIII GTNAC 3 cut(s) 613, 636, 756
MalI GATC 5 cut(s) 108, 228, 308, 582, 879
MboI GATC 5 cut(s) 106, 226, 306, 580, 877
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 1648
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 44, 731
MmeI TCCRAC 1 cut(s) 948
Mox20I TGGCCA 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1661
MscI TGGCCA 1 cut(s) 143
MseI TTAA 4 cut(s) 660, 1041, 1104, 1598
MslI CAYNNNNRTG 1 cut(s) 1083
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 1364
MspCI CTTAAG 1 cut(s) 1040
MspI CCGG 1 cut(s) 1579
MspR9I CCNGG 2 cut(s) 547, 1641
MvaI CCWGG 2 cut(s) 547, 1641
MwoI GCNNNNNNNGC 7 cut(s) 362, 980, 1058, 1262, 1331, 1370, 1391
NcoI CCATGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 1653
NdeII GATC 5 cut(s) 106, 226, 306, 580, 877
NlaIII CATG 8 cut(s) 233, 265, 301, 835, 844, 1082, 1144, 1246
NmeAIII GCCGAG 2 cut(s) 374, 1627
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 2 cut(s) 301, 1082
PaeI GCATGC 2 cut(s) 301, 1082
PagI TCATGA 1 cut(s) 261
PceI AGGCCT 1 cut(s) 1478
PciSI GCTCTTC 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 1661
PfeI GAWTC 3 cut(s) 128, 822, 1127
PflFI GACNNNGTC 1 cut(s) 53
PflMI CCANNNNNTGG 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 1639
PleI GAGTC 2 cut(s) 44, 731
PpsI GAGTC 2 cut(s) 44, 731
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 1648
Psp6I CCWGG 2 cut(s) 545, 1639
PspGI CCWGG 2 cut(s) 545, 1639
PspPI GGNCC 1 cut(s) 1465
PstI CTGCAG 3 cut(s) 535, 808, 1264
PstNI CAGNNNCTG 4 cut(s) 292, 1052, 1202, 1571
PsuI RGATCY 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 2 cut(s) 830, 1310
RsaNI GTAC 2 cut(s) 829, 1309
RseI CAYNNNNRTG 1 cut(s) 1083
SacI GAGCTC 1 cut(s) 1648
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 660, 1041, 1104, 1598
Sau3AI GATC 5 cut(s) 106, 226, 306, 580, 877
Sau96I GGNCC 1 cut(s) 1465
ScaI AGTACT 1 cut(s) 1310
SchI GAGTC 2 cut(s) 44, 731
ScrFI CCNGG 2 cut(s) 547, 1641
SduI GDGCHC 1 cut(s) 1648
SfaNI GCATC 7 cut(s) 76, 622, 992, 1039, 1045, 1503, 1577
SfcI CTRYAG 5 cut(s) 531, 650, 804, 1203, 1260
SinI GGWCC 1 cut(s) 1465
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 4 cut(s) 302, 1040, 1118, 1517
SmoI CTYRAG 4 cut(s) 302, 1040, 1118, 1517
SphI GCATGC 2 cut(s) 301, 1082
SseBI AGGCCT 1 cut(s) 1478
SsiI CCGC 4 cut(s) 363, 398, 785, 1059
SspI AATATT 1 cut(s) 1410
SspMI CTAG 5 cut(s) 446, 656, 779, 1251, 1482
SstI GAGCTC 1 cut(s) 1648
StuI AGGCCT 1 cut(s) 1478
StyD4I CCNGG 2 cut(s) 545, 1639
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 1 cut(s) 925
TaiI ACGT 1 cut(s) 1324
TaqI TCGA 3 cut(s) 22, 202, 309
TaqII GACCGA 2 cut(s) 43, 196
TatI WGTACW 2 cut(s) 828, 1308
TauI GCSGC 3 cut(s) 365, 788, 1061
TfiI GAWTC 3 cut(s) 128, 822, 1127
Tru1I TTAA 4 cut(s) 660, 1041, 1104, 1598
Tru9I TTAA 4 cut(s) 660, 1041, 1104, 1598
TscAI CASTG 3 cut(s) 540, 1090, 1402
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 7 cut(s) 803, 1094, 1163, 1262, 1361, 1364, 1565
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 6 cut(s) 267, 384, 447, 660, 878, 1670
TspGWI ACGGA 2 cut(s) 22, 655
TspRI CASTG 3 cut(s) 540, 1090, 1402
Tth111I GACNNNGTC 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 1040
VpaK11BI GGWCC 1 cut(s) 1465
XapI RAATTY 5 cut(s) 133, 479, 1154, 1536, 1594
XbaI TCTAGA 1 cut(s) 1481
XceI RCATGY 2 cut(s) 301, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 964
XmnI GAANNNNTTC 1 cut(s) 1661
XspI CTAG 5 cut(s) 446, 656, 779, 1251, 1482
ZrmI AGTACT 1 cut(s) 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.