AT1G68150
WRKY Family

DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011207
Physical Location & Seq
Forward (+)
25543949 .. 25545791
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68150.1

Sequence Viewer

Length: 1125 bp
ATGGGATTTGACTTTAGCACTTCAAAATCCAAAGCTAAAAGACAAAAAAGAATAGAGGTTCGATTTGCATCTCCATTAATGGGCATCGATCTTTCTCTTAAGCTCGAGGCCGAGGAGAAAAAGAAAGAGATAGAAGGATCGAAACATAGCCGTGAGAACAAAGAAGACGAAGAACATGATGCTAGTGGTGATGAAGATGAACAAATGGTGAAAGAAGACGAAGATGATTCTTCTTCTTTAGGTTTAAGAACCCGAGAAGAAGAAAACGAACGTGAAGAGCTCTTGCAGCTACAGATCCAGATGGAAAGTGTGAAAGAAGAGAATACTAGGTTGAGGAAGCTTGTCGAGCAGACTCTTGAAGATTATCGTCATCTTGAGATGAAATTCCCGGTTATCGATAAAACCAAGAAGATGGATCTTGAAATGTTCCTTGGAGTACAAGGCAAACGATGTGTGGATATAACAAGTAAGGCTCGGAAAAGAGGAGCTGAGAGATCTCCGTCAATGGAAAGAGAAATAGGGCTTTCACTTTCTCTAGAGAAAAAACAGAAACAAGAAGAGAGCAAAGAAGCTGTTCAGTCTCATCACCAAAGATACAATAGTAGCAGCTTAGATATGAATATGCCACGTATCATTTCATCTTCTCAAGGTAATAGAAAGGCCAGGGTGTCCGTGAGGGCGAGATGTGAGACCGCAACAATGAATGATGGATGCCAATGGAGGAAGTACGGTCAGAAAACCGCGAAAGGGAATCCATGTCCTCGAGCTTATTACCGATGCACCGTGGCTCCAGGATGTCCCGTTAGAAAACAGGTGCAAAGGTGTTTAGAAGACATGTCAATACTGATAACAACCTACGAAGGAACACATAACCATCCACTTCCGGTCGGAGCAACAGCCATGGCTTCCACTGCCTCTACTTCTCCATTCTTGTTACTCGATTCCAGTGACAACCTCTCTCATCCTTCCTATTACCAAACTCCTCAAGCCATAGACTCTTCTTTGATTACATACCCACAAAATAGCAGCTACAACAATCGAACCATAAGAAGCTTGAACTTTGATGGTCCATCTAGAGGAGATCACGTTTCATCTTCTCAAAACCGATTAAATTGGATGATGTAG

Protein Analysis

374

Amino Acids

42.74

Weight (kDa)

7.61

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 235 - 293 3.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 743
AciI CCGC 2 cut(s) 693, 741
AclWI GGATC 3 cut(s) 145, 289, 423
AcsI RAATTY 1 cut(s) 383
AfaI GTAC 2 cut(s) 438, 728
AfiI CCNNNNNNNGG 3 cut(s) 80, 747, 1076
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 1 cut(s) 834
AgsI TTSAA 4 cut(s) 24, 359, 422, 1057
AjnI CCWGG 2 cut(s) 662, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 2 cut(s) 585, 683
AlwI GGATC 3 cut(s) 145, 289, 423
Ama87I CYCGRG 3 cut(s) 104, 252, 762
AoxI GGCC 2 cut(s) 108, 660
ApeKI GCWGC 3 cut(s) 286, 606, 1026
ApoI RAATTY 1 cut(s) 383
AseI ATTAAT 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 573
AspS9I GGNCC 1 cut(s) 1067
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 3 cut(s) 200, 220, 578
AvaI CYCGRG 3 cut(s) 104, 252, 762
AvaII GGWCC 1 cut(s) 1067
BaeI ACNNNNGTAYC 2 cut(s) 586, 619
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 3 cut(s) 171, 222, 837
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 298, 618, 1038
BccI CCATC 6 cut(s) 295, 406, 701, 882, 1058, 1078
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 2 cut(s) 664, 792
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 585, 683
BfaI CTAG 4 cut(s) 183, 327, 536, 1074
BfmI CTRYAG 1 cut(s) 290
BfrI CTTAAG 1 cut(s) 98
BglII AGATCT 1 cut(s) 494
BisI GCNGC 3 cut(s) 287, 607, 1027
BlsI GCNGC 3 cut(s) 288, 608, 1028
Bme1390I CCNGG 3 cut(s) 389, 664, 792
Bme18I GGWCC 1 cut(s) 1067
BmeT110I CYCGRG 3 cut(s) 104, 252, 762
BmgT120I GGNCC 1 cut(s) 1067
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 3 cut(s) 389, 664, 792
BmsI GCATC 5 cut(s) 77, 93, 169, 701, 767
BpiI GAAGAC 3 cut(s) 171, 222, 837
BpmI CTGGAG 1 cut(s) 774
BpuEI CTTGAG 3 cut(s) 395, 630, 969
BpuMI CCSGG 1 cut(s) 389
Bsa29I ATCGAT 2 cut(s) 87, 396
BsaAI YACGTR 1 cut(s) 629
BsaBI GATNNNNATC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 683
BsaJI CCNNGG 5 cut(s) 111, 430, 663, 783, 900
BsaWI WCCGGW 1 cut(s) 883
BsaXI ACNNNNNCTCC 4 cut(s) 772, 802, 1071, 1101
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 747, 1076
Bse1I ACTGG 1 cut(s) 945
Bse8I GATNNNNATC 1 cut(s) 67
BseBI CCWGG 2 cut(s) 664, 792
BseCI ATCGAT 2 cut(s) 87, 396
BseDI CCNNGG 5 cut(s) 111, 430, 663, 783, 900
BseGI GGATG 5 cut(s) 716, 800, 874, 961, 1122
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 3 cut(s) 80, 747, 1076
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 1 cut(s) 945
BseRI GAGGAG 4 cut(s) 128, 498, 972, 1092
BseXI GCAGC 3 cut(s) 298, 618, 1038
Bsh1236I CGCG 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 888
BshFI GGCC 2 cut(s) 110, 662
BshVI ATCGAT 2 cut(s) 87, 396
BsiEI CGRYCG 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 3 cut(s) 104, 252, 762
BsiSI CCGG 2 cut(s) 389, 884
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 3 cut(s) 80, 747, 1076
BsmAI GTCTC 2 cut(s) 585, 683
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 2 cut(s) 110, 662
Bso31I GGTCTC 1 cut(s) 683
BsoBI CYCGRG 3 cut(s) 104, 252, 762
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 2 cut(s) 693, 741
BspANI GGCC 2 cut(s) 110, 662
BspCNI CTCAG 1 cut(s) 481
BspDI ATCGAT 2 cut(s) 87, 396
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 1 cut(s) 789
BspPI GGATC 3 cut(s) 145, 289, 423
BspQI GCTCTTC 1 cut(s) 270
BspTI CTTAAG 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 683
BsrI ACTGG 1 cut(s) 945
BssECI CCNNGG 5 cut(s) 111, 430, 663, 783, 900
BssMI GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
BssT1I CCWWGG 2 cut(s) 430, 900
Bst2UI CCWGG 2 cut(s) 664, 792
Bst4CI ACNGT 2 cut(s) 731, 784
Bst6I CTCTTC 4 cut(s) 270, 312, 552, 1003
BstAFI CTTAAG 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 629
BstDEI CTNAG 2 cut(s) 489, 610
BstDSI CCRYGG 2 cut(s) 783, 900
BstF5I GGATG 5 cut(s) 716, 800, 874, 961, 1122
BstFNI CGCG 1 cut(s) 743
BstKTI GATC 6 cut(s) 91, 140, 297, 418, 497, 1084
BstMAI GTCTC 2 cut(s) 585, 683
BstMBI GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
BstMCI CGRYCG 1 cut(s) 888
BstMWI GCNNNNNNNGC 3 cut(s) 286, 346, 911
BstNI CCWGG 2 cut(s) 664, 792
BstNSI RCATGY 1 cut(s) 838
BstSCI CCNGG 3 cut(s) 387, 662, 790
BstSFI CTRYAG 1 cut(s) 290
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 3 cut(s) 298, 618, 1038
BstV2I GAAGAC 3 cut(s) 171, 222, 837
BstX2I RGATCY 3 cut(s) 294, 415, 494
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 3 cut(s) 294, 415, 494
Bsu15I ATCGAT 2 cut(s) 87, 396
BsuRI GGCC 2 cut(s) 110, 662
BsuTUI ATCGAT 2 cut(s) 87, 396
BtgI CCRYGG 2 cut(s) 783, 900
BtsCI GGATG 5 cut(s) 716, 800, 874, 961, 1122
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 2 cut(s) 909, 952
Cfr13I GGNCC 1 cut(s) 1067
ClaI ATCGAT 2 cut(s) 87, 396
Csp6I GTAC 2 cut(s) 437, 727
CviAII CATG 4 cut(s) 176, 756, 835, 901
CviQI GTAC 2 cut(s) 437, 727
DdeI CTNAG 2 cut(s) 489, 610
DpnI GATC 6 cut(s) 90, 139, 296, 417, 496, 1083
DpnII GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
Eam1104I CTCTTC 4 cut(s) 270, 312, 552, 1003
EarI CTCTTC 4 cut(s) 270, 312, 552, 1003
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 430, 900
Eco24I GRGCYC 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 683
Eco47I GGWCC 1 cut(s) 1067
Eco53kI GAGCTC 1 cut(s) 280
Eco88I CYCGRG 3 cut(s) 104, 252, 762
EcoICRI GAGCTC 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 662, 790
EcoT14I CCWWGG 2 cut(s) 430, 900
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 430, 900
FaeI CATG 4 cut(s) 179, 759, 838, 904
FaqI GGGAC 1 cut(s) 783
FatI CATG 4 cut(s) 175, 755, 834, 900
Fnu4HI GCNGC 3 cut(s) 287, 607, 1027
FokI GGATG 4 cut(s) 723, 807, 861, 948
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 3 cut(s) 287, 607, 1027
FspBI CTAG 4 cut(s) 183, 327, 536, 1074
GluI GCNGC 3 cut(s) 287, 607, 1027
GsuI CTGGAG 1 cut(s) 774
HaeIII GGCC 2 cut(s) 110, 662
HapII CCGG 2 cut(s) 389, 884
Hin1II CATG 4 cut(s) 179, 759, 838, 904
HindIII AAGCTT 2 cut(s) 338, 1051
HinfI GANTC 5 cut(s) 227, 352, 751, 941, 995
HpaII CCGG 2 cut(s) 389, 884
HphI GGTGA 3 cut(s) 200, 220, 578
Hpy188I TCNGA 3 cut(s) 477, 735, 890
Hpy188III TCNNGA 6 cut(s) 298, 356, 374, 419, 536, 1074
HpyAV CCTTC 3 cut(s) 128, 854, 975
HpyCH4III ACNGT 2 cut(s) 731, 784
HpyCH4IV ACGT 3 cut(s) 271, 628, 1086
HpyCH4V TGCA 4 cut(s) 68, 286, 780, 817
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 346, 911
HpyF3I CTNAG 2 cut(s) 489, 610
HpySE526I ACGT 3 cut(s) 271, 628, 1086
Hsp92II CATG 4 cut(s) 179, 759, 838, 904
Kzo9I GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 3 cut(s) 485, 793, 890
LpnPI CCDG 9 cut(s) 311, 402, 649, 676, 777, 797, 804, 897, 958
Lsp1109I GCAGC 3 cut(s) 298, 618, 1038
LweI GCATC 5 cut(s) 77, 93, 169, 701, 767
MaeI CTAG 4 cut(s) 183, 327, 536, 1074
MaeII ACGT 3 cut(s) 271, 628, 1086
MaeIII GTNAC 2 cut(s) 933, 947
MalI GATC 6 cut(s) 90, 139, 296, 417, 496, 1083
MboI GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
MflI RGATCY 3 cut(s) 294, 415, 494
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 383, 1111
MlyI GAGTC 2 cut(s) 346, 989
MmeI TCCRAC 1 cut(s) 868
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 4 cut(s) 77, 99, 245, 1109
MslI CAYNNNNRTG 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 2 cut(s) 389, 884
MspR9I CCNGG 3 cut(s) 389, 664, 792
MvaI CCWGG 2 cut(s) 664, 792
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 3 cut(s) 286, 346, 911
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 900
NdeII GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
NlaIII CATG 4 cut(s) 179, 759, 838, 904
NlaIV GGNNCC 1 cut(s) 789
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 947
NspI RCATGY 1 cut(s) 838
PaeR7I CTCGAG 2 cut(s) 104, 762
PciI ACATGT 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 3 cut(s) 227, 751, 941
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 3 cut(s) 288, 608, 1028
PleI GAGTC 2 cut(s) 346, 989
PpsI GAGTC 2 cut(s) 346, 989
Ppu21I YACGTR 1 cut(s) 629
PscI ACATGT 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 662, 790
PspGI CCWGG 2 cut(s) 662, 790
PspN4I GGNNCC 1 cut(s) 789
PspPI GGNCC 1 cut(s) 1067
PspXI VCTCGAGB 2 cut(s) 104, 762
PsuI RGATCY 3 cut(s) 294, 415, 494
RsaI GTAC 2 cut(s) 438, 728
RsaNI GTAC 2 cut(s) 437, 727
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 4 cut(s) 77, 99, 245, 1109
SatI GCNGC 3 cut(s) 287, 607, 1027
Sau3AI GATC 6 cut(s) 88, 137, 294, 415, 494, 1081
Sau96I GGNCC 1 cut(s) 1067
SchI GAGTC 2 cut(s) 346, 989
ScrFI CCNGG 3 cut(s) 389, 664, 792
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 5 cut(s) 77, 93, 169, 701, 767
SfcI CTRYAG 1 cut(s) 290
Sfr274I CTCGAG 2 cut(s) 104, 762
SinI GGWCC 1 cut(s) 1067
SlaI CTCGAG 2 cut(s) 104, 762
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 6 cut(s) 98, 104, 374, 645, 762, 984
SmoI CTYRAG 6 cut(s) 98, 104, 374, 645, 762, 984
Sse9I AATT 2 cut(s) 383, 1111
SsiI CCGC 2 cut(s) 693, 741
SspMI CTAG 4 cut(s) 183, 327, 536, 1074
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 387, 662, 790
StyI CCWWGG 2 cut(s) 430, 900
TaaI ACNGT 2 cut(s) 731, 784
TaiI ACGT 3 cut(s) 274, 631, 1089
TaqI TCGA 9 cut(s) 61, 87, 105, 140, 345, 396, 763, 939, 1039
TasI AATT 2 cut(s) 383, 1111
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 3 cut(s) 227, 751, 941
Tru1I TTAA 4 cut(s) 77, 99, 245, 1109
Tru9I TTAA 4 cut(s) 77, 99, 245, 1109
TscAI CASTG 2 cut(s) 916, 952
TseFI GTSAC 1 cut(s) 947
TseI GCWGC 3 cut(s) 286, 606, 1026
Tsp45I GTSAC 1 cut(s) 947
TspDTI ATGAA 7 cut(s) 207, 213, 395, 627, 632, 716, 1080
TspGWI ACGGA 2 cut(s) 489, 661
TspRI CASTG 2 cut(s) 916, 952
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 1067
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 1 cut(s) 383
XbaI TCTAGA 2 cut(s) 535, 1073
XceI RCATGY 1 cut(s) 838
XhoI CTCGAG 2 cut(s) 104, 762
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 4 cut(s) 183, 327, 536, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.