FvH4_5g29820
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011207
Physical Location & Seq
Reverse (-)
20708528 .. 20711614
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29820.t3

Sequence Viewer

Length: 1566 bp
ATGGGGATCAGCAGAGAGAATGATCAGAAAGCAGACCTGGAAATTGGTCTTTCACTGAAGATTGATCATCCTTCTAAATCAGAACAAGAAGAAGAAGATGACGACAACCACGACGAAGACGAGAATATCCAAGAAGCATCAGCATCATTAGCAGCAGCAGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAATCAAACAGGATGATATTGAAGAAGAACAGAAAGCGGCTGCAGGTGAAGTGGATGATGATGCCTCAATAGTACTGGAGTCTTTGCATGAGAATAACATGAAGACAGATCAGGAGTTATACGCTTTGCAAATGGAAATGAGTCGCATGAAAGAAGAAAACAAGGTTTTGAGGAAGGTCGTGGAGCAAACCAAGAAGGATTATTACGATCTTCAAATGAAATTCACTGCTGTCCATCAGAATAGCCAAACCAATAAGGATCCTCATACATTTCTATCGCTTCATGGCAATGATCATGAAGAAGCTGATCCTCCAAAAAGTAGTATTCCCAAGACGTTGGATCTTCGTCGTCATAGATCTCCATCACCGCCGGCACGTGAGGCTGATGATATTATCAGAGAGAGTCAACTAGGGTTGTCACTGAGGCTACAAACCAACGAATTTAGGGATCAAAGAGAAGATCACGATGATATCCAAGAACGTAACAAGGAAGAATTGACGAGTAGTAGTCTCACATCATCAGTGCAACACAAGCTGCAGCGGACCGATTTGGCCGGAATTACTAGCCATGCCGCCCAAGCCAACAGAAAAGCTAGGGTTTCAGTTAGAGCAAGATGTGAATCTGCCACAATGAATGATGGGTGTCAATGGAGGAAATATGGGCAGAAAATCGCGAAAGGAAATCCTTGCCCCAGAGCCTATTACCGTTGCACAGTAGCTCCTGGATGCCCTGTTAGAAAACAGGTTCAAAGATGCCTAGAGGACATGTCCATACTGATAACAACCTACGAAGGAACACATAATCATCCACTTCCAGTTGGTGCAACTGCCATGGCTTCCACAGCTTCTGCAGCAGCTGCTTCCTTTATGTTACTAGATTCAAGCAATCACCATCTCTCAGATGGAACATTACCCTCCTCTAATTTCATGACCCAATCCTCCCTCCCTTCCAATAATTACTACAACACTCCTCACCCCATGTTAAACAACCAATACCCCTCTTCAATATCTCATCAATTTTCCTCCCCATCCAACTTCAGAACCCTAAGTTCCCTTCATCATCAGCCTCATCATCATGACCCTTCGAAAGGAATTGTGCTCGACCTCACGAACAACTTCGCTGATACTAGTACTAGTACAACTGCTATGGCTTCAGCTGGCTCTTCCTCCCACTCATCTGCACCGCCGGGGTTTAACAATTGGATGAGTAGCAAACCTGCAAAATATAACGCTGGTAACAGTACTACTATGATGATGGGCAACCATCATCTGCTGGGTAACAGTAGGAGGACGCCTGGGGAAGAAAATAATATGACGTCCTCCAGCTCGGCATTAATGGCTGAAAATCAGGGATAA

Protein Analysis

522

Amino Acids

58.09

Weight (kDa)

5.02

Isoelectric Point (pI)

60.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 282 - 340 1.1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 245
AatII GACGTC 1 cut(s) 1529
Acc36I ACCTGC 2 cut(s) 245, 1435
AccII CGCG 1 cut(s) 884
AciI CCGC 5 cut(s) 248, 578, 751, 783, 1394
AclWI GGATC 6 cut(s) 14, 464, 477, 512, 558, 666
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 2 cut(s) 431, 650
AcuI CTGAAG 3 cut(s) 77, 1231, 1347
AcvI CACGTG 1 cut(s) 587
AcyI GRCGYC 2 cut(s) 1502, 1526
AfaI GTAC 4 cut(s) 285, 1342, 1348, 1453
AfiI CCNNNNNNNGG 1 cut(s) 1298
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 5 cut(s) 233, 425, 959, 1092, 1215
AhlI ACTAGT 2 cut(s) 1337, 1343
AjnI CCWGG 3 cut(s) 36, 931, 1504
AluBI AGCT 8 cut(s) 515, 745, 803, 929, 1055, 1067, 1367, 1536
AluI AGCT 8 cut(s) 515, 745, 803, 929, 1055, 1067, 1367, 1536
Alw21I GWGCWC 1 cut(s) 1311
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 6 cut(s) 14, 464, 477, 512, 558, 666
AlwNI CAGNNNCTG 2 cut(s) 1058, 1067
AoxI GGCC 1 cut(s) 762
ApeKI GCWGC 8 cut(s) 152, 155, 251, 745, 748, 1061, 1064, 1067
ApoI RAATTY 2 cut(s) 431, 650
AseI ATTAAT 1 cut(s) 1544
Asp700I GAANNNNTTC 1 cut(s) 1325
AspS9I GGNCC 1 cut(s) 753
AsuC2I CCSGG 1 cut(s) 1398
AsuHPI GGTGA 4 cut(s) 269, 567, 1091, 1175
AsuII TTCGAA 1 cut(s) 1295
AvaII GGWCC 1 cut(s) 753
BamHI GGATCC 1 cut(s) 469
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbrPI CACGTG 1 cut(s) 587
BbsI GAAGAC 2 cut(s) 123, 320
Bbv12I GWGCWC 1 cut(s) 1311
BbvI GCAGC 8 cut(s) 164, 167, 238, 732, 760, 1054, 1073, 1076
BccI CCATC 8 cut(s) 453, 580, 842, 1106, 1110, 1246, 1459, 1482
BciT130I CCWGG 3 cut(s) 38, 933, 1506
BclI TGATCA 3 cut(s) 22, 64, 502
BcnI CCSGG 1 cut(s) 1398
BcoDI GTCTC 1 cut(s) 725
BcuI ACTAGT 2 cut(s) 1337, 1343
BfaI CTAG 7 cut(s) 620, 774, 804, 968, 1085, 1338, 1344
BfmI CTRYAG 3 cut(s) 252, 746, 1059
BfuAI ACCTGC 2 cut(s) 245, 1435
BglII AGATCT 1 cut(s) 566
BmcAI AGTACT 3 cut(s) 285, 1342, 1453
Bme1390I CCNGG 4 cut(s) 38, 933, 1398, 1506
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 753
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 4 cut(s) 38, 933, 1398, 1506
BmsI GCATC 5 cut(s) 146, 152, 262, 926, 953
BpiI GAAGAC 2 cut(s) 123, 320
BpmI CTGGAG 2 cut(s) 308, 1516
Bpu14I TTCGAA 1 cut(s) 1295
BpuMI CCSGG 1 cut(s) 1398
BsaAI YACGTR 1 cut(s) 587
BsaBI GATNNNNATC 2 cut(s) 571, 829
BsaHI GRCGYC 2 cut(s) 1502, 1526
BsaJI CCNNGG 3 cut(s) 1041, 1397, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 1 cut(s) 1298
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 2 cut(s) 291, 1025
Bse3DI GCAATG 1 cut(s) 505
Bse8I GATNNNNATC 2 cut(s) 571, 829
BseBI CCWGG 3 cut(s) 38, 933, 1506
BseDI CCNNGG 3 cut(s) 1041, 1397, 1505
BseGI GGATG 7 cut(s) 67, 229, 271, 941, 1015, 1238, 1419
BseJI GATNNNNATC 2 cut(s) 571, 829
BseLI CCNNNNNNNGG 1 cut(s) 1298
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 2 cut(s) 623, 1122
BseNI ACTGG 2 cut(s) 291, 1025
BseRI GAGGAG 2 cut(s) 1117, 1170
BseXI GCAGC 8 cut(s) 164, 167, 238, 732, 760, 1054, 1073, 1076
BseYI CCCAGC 1 cut(s) 1483
BsgI GTGCAG 1 cut(s) 1374
Bsh1236I CGCG 1 cut(s) 884
BshFI GGCC 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 1311
BsiSI CCGG 3 cut(s) 581, 765, 1397
BslI CCNNNNNNNGG 1 cut(s) 1298
BsmAI GTCTC 1 cut(s) 725
BsnI GGCC 1 cut(s) 764
Bsp119I TTCGAA 1 cut(s) 1295
Bsp1286I GDGCHC 1 cut(s) 1311
Bsp19I CCATGG 1 cut(s) 1041
Bsp68I TCGCGA 1 cut(s) 884
BspACI CCGC 5 cut(s) 248, 578, 751, 783, 1394
BspANI GGCC 1 cut(s) 764
BspCNI CTCAG 2 cut(s) 624, 1121
BspFNI CGCG 1 cut(s) 884
BspHI TCATGA 3 cut(s) 505, 1137, 1285
BspLI GGNNCC 1 cut(s) 471
BspMAI CTGCAG 3 cut(s) 256, 750, 1063
BspMI ACCTGC 2 cut(s) 245, 1435
BspPI GGATC 6 cut(s) 14, 464, 477, 512, 558, 666
BspQI GCTCTTC 1 cut(s) 1378
BspT104I TTCGAA 1 cut(s) 1295
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 2 cut(s) 291, 1025
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 3 cut(s) 1041, 1397, 1505
BssNI GRCGYC 2 cut(s) 1502, 1526
BssT1I CCWWGG 1 cut(s) 1041
Bst2UI CCWGG 3 cut(s) 38, 933, 1506
Bst4CI ACNGT 4 cut(s) 917, 925, 1451, 1493
Bst6I CTCTTC 2 cut(s) 1216, 1378
BstACI GRCGYC 2 cut(s) 1502, 1526
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstBAI YACGTR 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 1295
BstC8I GCNNGC 2 cut(s) 582, 1369
BstDEI CTNAG 3 cut(s) 632, 1108, 1256
BstDSI CCRYGG 1 cut(s) 1041
BstENI CCTNNNNNAGG 1 cut(s) 1296
BstF5I GGATG 7 cut(s) 67, 229, 271, 941, 1015, 1238, 1419
BstFNI CGCG 1 cut(s) 884
BstMAI GTCTC 1 cut(s) 725
BstMWI GCNNNNNNNGC 8 cut(s) 149, 590, 742, 788, 1052, 1061, 1067, 1547
BstNI CCWGG 3 cut(s) 38, 933, 1506
BstNSI RCATGY 1 cut(s) 979
BstSCI CCNGG 4 cut(s) 36, 931, 1396, 1504
BstSFI CTRYAG 3 cut(s) 252, 746, 1059
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 8 cut(s) 164, 167, 238, 732, 760, 1054, 1073, 1076
BstV2I GAAGAC 2 cut(s) 123, 320
BstX2I RGATCY 3 cut(s) 469, 550, 566
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 3 cut(s) 469, 550, 566
BsuRI GGCC 1 cut(s) 764
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 7 cut(s) 67, 229, 271, 941, 1015, 1238, 1419
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 4 cut(s) 53, 435, 629, 738
BtuMI TCGCGA 1 cut(s) 884
BveI ACCTGC 2 cut(s) 245, 1435
Cac8I GCNNGC 2 cut(s) 582, 1369
CaiI CAGNNNCTG 2 cut(s) 1058, 1067
CciI TCATGA 3 cut(s) 505, 1137, 1285
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 753
CpoI CGGWCCG 1 cut(s) 753
CseI GACGC 1 cut(s) 1510
Csp6I GTAC 4 cut(s) 284, 1341, 1347, 1452
CspI CGGWCCG 1 cut(s) 753
CviQI GTAC 4 cut(s) 284, 1341, 1347, 1452
DdeI CTNAG 3 cut(s) 632, 1108, 1256
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 1216, 1378
EarI CTCTTC 2 cut(s) 1216, 1378
Eco130I CCWWGG 1 cut(s) 1041
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 3 cut(s) 77, 1231, 1347
Eco72I CACGTG 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 1296
EcoRII CCWGG 3 cut(s) 36, 931, 1504
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 1041
ErhI CCWWGG 1 cut(s) 1041
FbaI TGATCA 3 cut(s) 22, 64, 502
FokI GGATG 7 cut(s) 54, 236, 278, 948, 1002, 1225, 1426
FspBI CTAG 7 cut(s) 620, 774, 804, 968, 1085, 1338, 1344
GsaI CCCAGC 1 cut(s) 1487
GsuI CTGGAG 2 cut(s) 308, 1516
HaeIII GGCC 1 cut(s) 764
HapII CCGG 3 cut(s) 581, 765, 1397
HgaI GACGC 1 cut(s) 1510
Hin1I GRCGYC 2 cut(s) 1502, 1526
HincII GTYRAC 1 cut(s) 617
HindII GTYRAC 1 cut(s) 617
HinfI GANTC 5 cut(s) 290, 352, 613, 830, 1088
HpaII CCGG 3 cut(s) 581, 765, 1397
HphI GGTGA 4 cut(s) 269, 567, 1091, 1175
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 6 cut(s) 27, 82, 450, 608, 1111, 1250
Hpy188III TCNNGA 7 cut(s) 323, 506, 674, 883, 1138, 1286, 1318
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 2 cut(s) 116, 561
HpyAV CCTTC 7 cut(s) 81, 379, 400, 995, 1167, 1274, 1302
HpyCH4III ACNGT 4 cut(s) 917, 925, 1451, 1493
HpyCH4IV ACGT 4 cut(s) 545, 586, 691, 1526
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 590, 742, 788, 1052, 1061, 1067, 1547
HpyF3I CTNAG 3 cut(s) 632, 1108, 1256
HpySE526I ACGT 4 cut(s) 545, 586, 691, 1526
Hsp92I GRCGYC 2 cut(s) 1502, 1526
KroI GCCGGC 1 cut(s) 580
KroNI GCCGGC 1 cut(s) 582
Ksp22I TGATCA 3 cut(s) 22, 64, 502
LguI GCTCTTC 1 cut(s) 1378
LmnI GCTCC 2 cut(s) 394, 934
Lsp1109I GCAGC 8 cut(s) 164, 167, 238, 732, 760, 1054, 1073, 1076
LweI GCATC 5 cut(s) 146, 152, 262, 926, 953
MaeI CTAG 7 cut(s) 620, 774, 804, 968, 1085, 1338, 1344
MaeII ACGT 4 cut(s) 545, 586, 691, 1526
MaeIII GTNAC 5 cut(s) 627, 692, 1080, 1445, 1487
MfeI CAATTG 1 cut(s) 1408
MflI RGATCY 3 cut(s) 469, 550, 566
MhlI GDGCHC 1 cut(s) 1311
MlyI GAGTC 3 cut(s) 299, 361, 622
MmeI TCCRAC 2 cut(s) 528, 1266
MroNI GCCGGC 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 1325
MseI TTAA 3 cut(s) 1193, 1404, 1544
MslI CAYNNNNRTG 2 cut(s) 498, 1284
MspA1I CMGCKG 3 cut(s) 751, 1067, 1367
MspI CCGG 3 cut(s) 581, 765, 1397
MspR9I CCNGG 4 cut(s) 38, 933, 1398, 1506
MunI CAATTG 1 cut(s) 1408
MvaI CCWGG 3 cut(s) 38, 933, 1506
MvnI CGCG 1 cut(s) 884
MwoI GCNNNNNNNGC 8 cut(s) 149, 590, 742, 788, 1052, 1061, 1067, 1547
NaeI GCCGGC 1 cut(s) 582
NciI CCSGG 1 cut(s) 1398
NcoI CCATGG 1 cut(s) 1041
NgoMIV GCCGGC 1 cut(s) 580
NlaIV GGNNCC 1 cut(s) 471
NmeAIII GCCGAG 1 cut(s) 1517
NmuCI GTSAC 1 cut(s) 627
NruI TCGCGA 1 cut(s) 884
NspI RCATGY 1 cut(s) 979
NspV TTCGAA 1 cut(s) 1295
PagI TCATGA 3 cut(s) 505, 1137, 1285
PaqCI CACCTGC 1 cut(s) 245
PciI ACATGT 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 1378
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 117
PdiI GCCGGC 1 cut(s) 582
PdmI GAANNNNTTC 1 cut(s) 1325
PfeI GAWTC 2 cut(s) 830, 1088
PfoI TCCNGGA 1 cut(s) 931
PleI GAGTC 3 cut(s) 298, 360, 621
PmaCI CACGTG 1 cut(s) 587
PmlI CACGTG 1 cut(s) 587
PpsI GAGTC 3 cut(s) 298, 360, 621
Ppu21I YACGTR 1 cut(s) 587
PscI ACATGT 1 cut(s) 975
PshBI ATTAAT 1 cut(s) 1544
Psp6I CCWGG 3 cut(s) 36, 931, 1504
PspCI CACGTG 1 cut(s) 587
PspFI CCCAGC 1 cut(s) 1483
PspGI CCWGG 3 cut(s) 36, 931, 1504
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 753
PstI CTGCAG 3 cut(s) 256, 750, 1063
PstNI CAGNNNCTG 2 cut(s) 1058, 1067
PsuI RGATCY 3 cut(s) 469, 550, 566
PvuII CAGCTG 2 cut(s) 1067, 1367
RruI TCGCGA 1 cut(s) 884
RsaI GTAC 4 cut(s) 285, 1342, 1348, 1453
RsaNI GTAC 4 cut(s) 284, 1341, 1347, 1452
RseI CAYNNNNRTG 2 cut(s) 498, 1284
Rsr2I CGGWCCG 1 cut(s) 753
RsrII CGGWCCG 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 1378
SaqAI TTAA 3 cut(s) 1193, 1404, 1544
Sau96I GGNCC 1 cut(s) 753
ScaI AGTACT 3 cut(s) 285, 1342, 1453
SchI GAGTC 3 cut(s) 299, 361, 622
ScrFI CCNGG 4 cut(s) 38, 933, 1398, 1506
SduI GDGCHC 1 cut(s) 1311
SfaNI GCATC 5 cut(s) 146, 152, 262, 926, 953
SfcI CTRYAG 3 cut(s) 252, 746, 1059
SfuI TTCGAA 1 cut(s) 1295
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 2 cut(s) 498, 1284
SpeI ACTAGT 2 cut(s) 1337, 1343
SsiI CCGC 5 cut(s) 248, 578, 751, 783, 1394
SspMI CTAG 7 cut(s) 620, 774, 804, 968, 1085, 1338, 1344
StyD4I CCNGG 4 cut(s) 36, 931, 1396, 1504
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 4 cut(s) 917, 925, 1451, 1493
TaiI ACGT 4 cut(s) 548, 589, 694, 1529
TaqI TCGA 2 cut(s) 1295, 1311
TaqII GACCGA 1 cut(s) 770
TatI WGTACW 4 cut(s) 283, 1340, 1346, 1451
TauI GCSGC 2 cut(s) 251, 785
TfiI GAWTC 2 cut(s) 830, 1088
Tru1I TTAA 3 cut(s) 1193, 1404, 1544
Tru9I TTAA 3 cut(s) 1193, 1404, 1544
TscAI CASTG 4 cut(s) 60, 442, 636, 738
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 8 cut(s) 152, 155, 251, 745, 748, 1061, 1064, 1067
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 9 cut(s) 177, 326, 374, 443, 482, 522, 857, 1126, 1256
TspRI CASTG 4 cut(s) 60, 442, 636, 738
VpaK11BI GGWCC 1 cut(s) 753
VspI ATTAAT 1 cut(s) 1544
XagI CCTNNNNNAGG 1 cut(s) 1296
XapI RAATTY 2 cut(s) 431, 650
XceI RCATGY 1 cut(s) 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XmnI GAANNNNTTC 1 cut(s) 1325
XspI CTAG 7 cut(s) 620, 774, 804, 968, 1085, 1338, 1344
ZraI GACGTC 1 cut(s) 1527
ZrmI AGTACT 3 cut(s) 285, 1342, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.