Rw7G030590
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011207
Physical Location & Seq
Reverse (-)
43415476 .. 43418137
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030590.1

Sequence Viewer

Length: 1653 bp
ATGGGGATCAGCAGAGAGAATGATCAGAACGACTTGGAAATTGGTCTTTCACTGAAAATTGATCACCCTCCTAAAAAAGAACAAGAAGAAGAAGATGGCAACGACGACGAAGACGAAGTCCACGAGGATATCGAAGAAGAATCAGCAGCAGAAGAAGAAGAGATCAAAGAGGATAATATTGAAGAAGAACCAAAAGCGGCTGCAGGTGAAGTGGATGATGATGCCTCAGTAGTACTGGAGACAACAAATTTGCATGAGAATAACTTGAAGACAGATCAGGAGTTATGCTCTTTGCAAATGGAGATGAGCCACATGAAAGAAGAAAATAAGGTTTTGAGGAAGGTCGTGGAGCAAACCATGAAGGATTATTACGATCTTCAAATGAAATTCACAGCTGTCCATCAAAATAGCCAAACCAAGGATCCTCAGACCTTTTTATCGCTTCGTGGTAATGATCATGATGAAGCTGATCCTCCAAAAAGTAGTATTCCCAAGATGTCGGAAATTCGTCGTCATAGATCTCCATCACCGGCACGTGAGGATGATATCATAAAAGAGAGTCAACTAGGGCTGTCACTGAGGCTACAAACCAATGAATTTAAGGATCAGAGAGAAGATCACGATATCCAAGAACGTAACAAGGAAGAATTGACGAGTAGTAGTCTCACATCATCAGTGCAACACAAGCTGCAGCGGACCGATTTGGCCGGAATTACTAGCCATGCCGCCCAAGCCAACAGAAAAGCTAGGGTTTCGGTTAGAGCAAGATGTGAATCTGCCACAATGAATGATGGGTGTCAATGGAGGAAATATGGGCAGAAAATCGCGAAAGGAAACCCTTGCCCCAGAGCCTATTACCGTTGCACAGTAGCTCCTGGATGTCCTGTTAGAAAACAGGTCCAAAGATGCCTAGAGGACATGTCCATACTGATAACAACATACGAAGGAACACATAATCATCCACTTCCAGTTGGTGCAACTGCCATGGCTTCCACAGCTTCTGCAGCAGCTGCTTCCTTTATGTTACTAGATTCAAGCAACCACCATCTCTCAGATGGAACACAACCCTCCTCTAATTTCATGACCCAAGCCTCCCTCCCTCCCAATAATTACTACAACACCCCTCACCACATGTTAAACAACCTCTACCCCTCATCAATATCTCATCAATATTCCTCCTCATCAAACTTCCGAACCCTAAATTCCGTTCATCATCAGCCTCATCATGACCCTTCAAAAGGAATCGTTCTCGACCTCACCAACAACTTCAGTGATACTTCTACTAGTACTGCCATAGCTTCAGCTGGCTCTTCCTCCCATTCATCTGCACCGCTGGGGTTTAACAATTGGATGGGTGGCAAACCTGCAAATTATTACCATAATGCTGGTAATAGTACGATGATAATGGGCAACCATCATCTGCTGAGTAACAGCAGGAGGACATCTGGGGAAGAAAGTATGACGTCGTCGTCAGCATTAATGGCTGAAAATGTGAGTGCTATTGCTTCAGATCCTAAATTTAGGGTTGCTGTTGCAGCTGCTATTACATCTCTCATTAATAGTAAAGAGAATAATACAACTCATCATCCTACTCCTCCTCATAATAATAATAATTTTGGTCCAAGGGATAATGGAGAGAAAGGTGGTAGCTAA

Protein Analysis

550

Amino Acids

60.9

Weight (kDa)

5.49

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 263 - 321 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
AasI GACNNNNNNGTC 1 cut(s) 1468
AatII GACGTC 1 cut(s) 1466
Acc36I ACCTGC 2 cut(s) 194, 1372
AccII CGCG 1 cut(s) 827
AciI CCGC 4 cut(s) 197, 694, 726, 1331
AclWI GGATC 6 cut(s) 14, 416, 429, 464, 612, 1505
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 6 cut(s) 247, 386, 504, 596, 1201, 1517
AcuI CTGAAG 3 cut(s) 1252, 1284, 1491
AcvI CACGTG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 1463
AfaI GTAC 3 cut(s) 234, 1288, 1396
AfiI CCNNNNNNNGG 2 cut(s) 418, 1238
AflIII ACRYGT 2 cut(s) 918, 1131
AgsI TTSAA 5 cut(s) 182, 268, 380, 1035, 1236
AhlI ACTAGT 1 cut(s) 1283
AjnI CCWGG 1 cut(s) 874
Alw26I GTCTC 2 cut(s) 233, 668
AlwI GGATC 6 cut(s) 14, 416, 429, 464, 612, 1505
AlwNI CAGNNNCTG 2 cut(s) 1001, 1010
AoxI GGCC 1 cut(s) 705
ApeKI GCWGC 9 cut(s) 146, 200, 688, 691, 1004, 1007, 1010, 1535, 1538
ApoI RAATTY 6 cut(s) 247, 386, 504, 596, 1201, 1517
ArsI GACNNNNNNTTYG 4 cut(s) 102, 134, 232, 264
AseI ATTAAT 2 cut(s) 1478, 1557
AspS9I GGNCC 3 cut(s) 696, 898, 1619
AsuHPI GGTGA 5 cut(s) 56, 218, 519, 1118, 1249
AvaII GGWCC 3 cut(s) 696, 898, 1619
BamHI GGATCC 1 cut(s) 421
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 1 cut(s) 122
BbrPI CACGTG 1 cut(s) 536
BbsI GAAGAC 2 cut(s) 117, 275
BbvI GCAGC 9 cut(s) 158, 187, 675, 703, 997, 1016, 1019, 1525, 1547
BccI CCATC 8 cut(s) 89, 408, 532, 785, 1049, 1053, 1345, 1422
BciT130I CCWGG 1 cut(s) 876
BclI TGATCA 3 cut(s) 22, 61, 454
BcoDI GTCTC 2 cut(s) 233, 668
BcuI ACTAGT 1 cut(s) 1283
BfaI CTAG 6 cut(s) 566, 717, 747, 911, 1028, 1284
BfmI CTRYAG 3 cut(s) 201, 689, 1002
BfuAI ACCTGC 2 cut(s) 194, 1372
BglII AGATCT 1 cut(s) 518
BmcAI AGTACT 2 cut(s) 234, 1288
Bme1390I CCNGG 1 cut(s) 876
Bme18I GGWCC 3 cut(s) 696, 898, 1619
BmgT120I GGNCC 3 cut(s) 696, 898, 1619
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 1 cut(s) 876
BmsI GCATC 2 cut(s) 211, 896
BpiI GAAGAC 2 cut(s) 117, 275
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 536
BsaBI GATNNNNATC 2 cut(s) 523, 772
BsaHI GRCGYC 1 cut(s) 1463
BsaJI CCNNGG 3 cut(s) 417, 984, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 856, 886
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 1238
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 2 cut(s) 240, 968
Bse8I GATNNNNATC 2 cut(s) 523, 772
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 3 cut(s) 417, 984, 1622
BseGI GGATG 6 cut(s) 220, 547, 884, 958, 1356, 1585
BseJI GATNNNNATC 2 cut(s) 523, 772
BseLI CCNNNNNNNGG 2 cut(s) 418, 1238
BseMII CTCAG 5 cut(s) 240, 440, 569, 1065, 1415
BseNI ACTGG 2 cut(s) 240, 968
BseRI GAGGAG 4 cut(s) 1060, 1168, 1584, 1587
BseXI GCAGC 9 cut(s) 158, 187, 675, 703, 997, 1016, 1019, 1525, 1547
BseYI CCCAGC 1 cut(s) 1333
BsgI GTGCAG 1 cut(s) 1311
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 1 cut(s) 707
BsiSI CCGG 2 cut(s) 530, 708
BslI CCNNNNNNNGG 2 cut(s) 418, 1238
BsmAI GTCTC 2 cut(s) 233, 668
BsnI GGCC 1 cut(s) 707
Bsp19I CCATGG 1 cut(s) 984
Bsp68I TCGCGA 1 cut(s) 827
BspACI CCGC 4 cut(s) 197, 694, 726, 1331
BspANI GGCC 1 cut(s) 707
BspCNI CTCAG 5 cut(s) 239, 439, 570, 1064, 1416
BspFNI CGCG 1 cut(s) 827
BspHI TCATGA 3 cut(s) 457, 1080, 1225
BspLI GGNNCC 1 cut(s) 423
BspMAI CTGCAG 3 cut(s) 205, 693, 1006
BspMI ACCTGC 2 cut(s) 194, 1372
BspPI GGATC 6 cut(s) 14, 416, 429, 464, 612, 1505
BspQI GCTCTTC 1 cut(s) 1315
BsrFI RCCGGY 1 cut(s) 529
BsrI ACTGG 2 cut(s) 240, 968
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 3 cut(s) 417, 984, 1622
BssNI GRCGYC 1 cut(s) 1463
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 3 cut(s) 417, 984, 1622
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 2 cut(s) 860, 868
Bst6I CTCTTC 2 cut(s) 153, 1315
BstACI GRCGYC 1 cut(s) 1463
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstBAI YACGTR 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 1306
BstDEI CTNAG 5 cut(s) 226, 426, 578, 1051, 1424
BstDSI CCRYGG 1 cut(s) 984
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 6 cut(s) 220, 547, 884, 958, 1356, 1585
BstFNI CGCG 1 cut(s) 827
BstMAI GTCTC 2 cut(s) 233, 668
BstMWI GCNNNNNNNGC 7 cut(s) 685, 731, 995, 1004, 1010, 1481, 1535
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 2 cut(s) 922, 1135
BstSCI CCNGG 1 cut(s) 874
BstSFI CTRYAG 3 cut(s) 201, 689, 1002
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 9 cut(s) 158, 187, 675, 703, 997, 1016, 1019, 1525, 1547
BstV2I GAAGAC 2 cut(s) 117, 275
BstX2I RGATCY 3 cut(s) 421, 518, 1510
BstXI CCANNNNNNTGG 1 cut(s) 1385
BstYI RGATCY 3 cut(s) 421, 518, 1510
BsuRI GGCC 1 cut(s) 707
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 6 cut(s) 220, 547, 884, 958, 1356, 1585
BtsIMutI CAGTG 4 cut(s) 50, 575, 681, 1276
BtuMI TCGCGA 1 cut(s) 827
BveI ACCTGC 2 cut(s) 194, 1372
Cac8I GCNNGC 1 cut(s) 1306
CaiI CAGNNNCTG 2 cut(s) 1001, 1010
CciI TCATGA 3 cut(s) 457, 1080, 1225
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 3 cut(s) 696, 898, 1619
CpoI CGGWCCG 1 cut(s) 696
Csp6I GTAC 3 cut(s) 233, 1287, 1395
CspI CGGWCCG 1 cut(s) 696
CviQI GTAC 3 cut(s) 233, 1287, 1395
DdeI CTNAG 5 cut(s) 226, 426, 578, 1051, 1424
DrdI GACNNNNNNGTC 1 cut(s) 1468
DseDI GACNNNNNNGTC 1 cut(s) 1468
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 2 cut(s) 153, 1315
EarI CTCTTC 2 cut(s) 153, 1315
Eco130I CCWWGG 3 cut(s) 417, 984, 1622
Eco32I GATATC 3 cut(s) 130, 547, 625
Eco47I GGWCC 3 cut(s) 696, 898, 1619
Eco57I CTGAAG 3 cut(s) 1252, 1284, 1491
Eco72I CACGTG 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoRII CCWGG 1 cut(s) 874
EcoRV GATATC 3 cut(s) 130, 547, 625
EcoT14I CCWWGG 3 cut(s) 417, 984, 1622
ErhI CCWWGG 3 cut(s) 417, 984, 1622
FbaI TGATCA 3 cut(s) 22, 61, 454
FokI GGATG 6 cut(s) 227, 554, 891, 945, 1363, 1572
FspBI CTAG 6 cut(s) 566, 717, 747, 911, 1028, 1284
GsaI CCCAGC 1 cut(s) 1337
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 707
HapII CCGG 2 cut(s) 530, 708
Hin1I GRCGYC 1 cut(s) 1463
HincII GTYRAC 1 cut(s) 563
HindII GTYRAC 1 cut(s) 563
HinfI GANTC 5 cut(s) 140, 559, 773, 1031, 1242
HpaII CCGG 2 cut(s) 530, 708
HphI GGTGA 5 cut(s) 56, 218, 519, 1118, 1249
Hpy166II GTNNAC 2 cut(s) 121, 563
Hpy188I TCNGA 7 cut(s) 27, 429, 502, 609, 1054, 1193, 1510
Hpy188III TCNNGA 7 cut(s) 278, 458, 620, 826, 1081, 1226, 1250
Hpy8I GTNNAC 2 cut(s) 121, 563
Hpy99I CGWCG 5 cut(s) 107, 110, 513, 1468, 1471
HpyAV CCTTC 4 cut(s) 334, 355, 938, 1242
HpyCH4III ACNGT 2 cut(s) 860, 868
HpyCH4IV ACGT 3 cut(s) 535, 634, 1463
HpyF10VI GCNNNNNNNGC 7 cut(s) 685, 731, 995, 1004, 1010, 1481, 1535
HpyF3I CTNAG 5 cut(s) 226, 426, 578, 1051, 1424
HpySE526I ACGT 3 cut(s) 535, 634, 1463
Hsp92I GRCGYC 1 cut(s) 1463
Ksp22I TGATCA 3 cut(s) 22, 61, 454
LguI GCTCTTC 1 cut(s) 1315
LmnI GCTCC 2 cut(s) 349, 877
Lsp1109I GCAGC 9 cut(s) 158, 187, 675, 703, 997, 1016, 1019, 1525, 1547
LweI GCATC 2 cut(s) 211, 896
MaeI CTAG 6 cut(s) 566, 717, 747, 911, 1028, 1284
MaeII ACGT 3 cut(s) 535, 634, 1463
MaeIII GTNAC 4 cut(s) 573, 635, 1023, 1427
MfeI CAATTG 1 cut(s) 1345
MflI RGATCY 3 cut(s) 421, 518, 1510
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 1 cut(s) 480
MseI TTAA 5 cut(s) 600, 1136, 1341, 1478, 1557
MspA1I CMGCKG 6 cut(s) 395, 694, 1010, 1304, 1333, 1538
MspI CCGG 2 cut(s) 530, 708
MspR9I CCNGG 1 cut(s) 876
MunI CAATTG 1 cut(s) 1345
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 7 cut(s) 685, 731, 995, 1004, 1010, 1481, 1535
NcoI CCATGG 1 cut(s) 984
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 573
NruI TCGCGA 1 cut(s) 827
NspI RCATGY 2 cut(s) 922, 1135
PagI TCATGA 3 cut(s) 457, 1080, 1225
PaqCI CACCTGC 1 cut(s) 194
PciI ACATGT 2 cut(s) 918, 1131
PciSI GCTCTTC 1 cut(s) 1315
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 120, 129
PfeI GAWTC 4 cut(s) 140, 773, 1031, 1242
PflFI GACNNNGTC 2 cut(s) 116, 1465
PfoI TCCNGGA 1 cut(s) 874
PleI GAGTC 1 cut(s) 567
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
PpsI GAGTC 1 cut(s) 567
Ppu21I YACGTR 1 cut(s) 536
PscI ACATGT 2 cut(s) 918, 1131
PshBI ATTAAT 2 cut(s) 1478, 1557
Psp6I CCWGG 1 cut(s) 874
PspCI CACGTG 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 1333
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 3 cut(s) 696, 898, 1619
PstI CTGCAG 3 cut(s) 205, 693, 1006
PstNI CAGNNNCTG 2 cut(s) 1001, 1010
PsuI RGATCY 3 cut(s) 421, 518, 1510
PsyI GACNNNGTC 2 cut(s) 116, 1465
PvuII CAGCTG 4 cut(s) 395, 1010, 1304, 1538
RruI TCGCGA 1 cut(s) 827
RsaI GTAC 3 cut(s) 234, 1288, 1396
RsaNI GTAC 3 cut(s) 233, 1287, 1395
Rsr2I CGGWCCG 1 cut(s) 696
RsrII CGGWCCG 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 1315
SaqAI TTAA 5 cut(s) 600, 1136, 1341, 1478, 1557
Sau96I GGNCC 3 cut(s) 696, 898, 1619
ScaI AGTACT 2 cut(s) 234, 1288
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 1 cut(s) 876
SfaNI GCATC 2 cut(s) 211, 896
SfcI CTRYAG 3 cut(s) 201, 689, 1002
SinI GGWCC 3 cut(s) 696, 898, 1619
SpeI ACTAGT 1 cut(s) 1283
SsiI CCGC 4 cut(s) 197, 694, 726, 1331
SspI AATATT 2 cut(s) 178, 1172
SspMI CTAG 6 cut(s) 566, 717, 747, 911, 1028, 1284
StyD4I CCNGG 1 cut(s) 874
StyI CCWWGG 3 cut(s) 417, 984, 1622
TaaI ACNGT 2 cut(s) 860, 868
TaiI ACGT 3 cut(s) 538, 637, 1466
TaqI TCGA 2 cut(s) 132, 1251
TaqII GACCGA 1 cut(s) 713
TatI WGTACW 2 cut(s) 232, 1286
TauI GCSGC 2 cut(s) 200, 728
TfiI GAWTC 4 cut(s) 140, 773, 1031, 1242
Tru1I TTAA 5 cut(s) 600, 1136, 1341, 1478, 1557
Tru9I TTAA 5 cut(s) 600, 1136, 1341, 1478, 1557
TscAI CASTG 4 cut(s) 57, 582, 681, 1276
TseFI GTSAC 1 cut(s) 573
TseI GCWGC 9 cut(s) 146, 200, 688, 691, 1004, 1007, 1010, 1535, 1538
Tsp45I GTSAC 1 cut(s) 573
TspDTI ATGAA 9 cut(s) 329, 374, 398, 477, 609, 800, 1069, 1199, 1311
TspGWI ACGGA 1 cut(s) 1195
TspRI CASTG 4 cut(s) 57, 582, 681, 1276
Tth111I GACNNNGTC 2 cut(s) 116, 1465
VpaK11BI GGWCC 3 cut(s) 696, 898, 1619
VspI ATTAAT 2 cut(s) 1478, 1557
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 6 cut(s) 247, 386, 504, 596, 1201, 1517
XceI RCATGY 2 cut(s) 922, 1135
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XspI CTAG 6 cut(s) 566, 717, 747, 911, 1028, 1284
ZraI GACGTC 1 cut(s) 1464
ZrmI AGTACT 2 cut(s) 234, 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.