RLG00000001986
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011207
Physical Location & Seq
Forward (+)
23941102 .. 23943763
2662 bp
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UTR
Exon/CDS
Intron
RLM00000001986

Sequence Viewer

Length: 1671 bp
ATGGGGATCAGCAGAGAGAATGATCAGAACGACTTGGAAATTAGTCTTTCACTGAAAATTGATCACCCTCCTAAAAAAGAACAAGAAGAAGAAGATGACAACGACGACGAAGACGAAGTCCACGAGGATACCGAAGAAGAATCACCAGCAGGAGCAACAGCAGCAGTAGAAGAAGAAGAGATCAAAGAGGATAATATTGAAGAAGAACCAAAAGCGGCTGTAGGTGAAGTGGATGATGATGCCTCAGTAGTACTGGAGACAACATATTTGCATGAGAATAACTTGAAGACAGATCAGGAGTTATGCGCTTTGCAAATGGAGATGAGTCGCATGAAAGAAGAAAATAAGGTTTTGAGGAAGGTCGTGGAGCAAACCATGAAGGATTATTACGATCTTCAAATGAAATTCACAGCTGTCCATCAAAATAGCCAAACCAAGGATCCTCAGACCTTTTTATCGCTTCGTGGTAATGATCATGATGAAGCTGATCCTCCAAAACGTAGTATTCCCAAGATGTCCGATATTCGTCGTCATAGATCACCATCACCGGCACGTGAGGATGATATTATAAAAGAGAGTCAACTAGGGCTGTCACTGAGGCTACAAACCAATGAATTTAGGGATCAGAGAGAAGATCATGATATCCAAGAACGTAACAAGGAAGAATTGCCGAGTAGTAGTCTCACATCATCAGTGCAACACAAGCTGCAGCGGACCGATTTGGCCGGAATTACTAGCCATGCCGCCCAAGCCAACAGAAAAGCTAGGGTTTCGGTTAGAGCAAGATGTGAATCTGCCACAATGAATGATGGGTGTCAATGGAGGAAATATGGGCAGAAAATCGCGAAAGGAAACCCTTGCCCCAGAGCCTATTACCGTTGCACAGTAGCTCCTGGATGTCCTGTTAGAAAACAGGTCCAAAGATGCCTAGAGGACATGTCCATACTGATAACAACATACGAAGGAACACATAATCATCCACTTCCAGTTGGTGCAACTGCCATGTCTTCCACAGCTTCTGCAGCAGCTGCTTCCTTTATGTTACTAGATTCAAGCAACCACCATCTCTCAGATGGAACACTACCCTCCTCTAATTTCATGACCCAAGCCTCCCTCCCTCCCAATAATTACTACAACACCCCTCACCACATGGTAACCAACCACTACCCCTCATCAATATCTCATCAATATTCCTCCTCATCAAACTTCCGAACCCTAAATTCCGTTCATCATCAGCCTCATCATGACCCTTCAAAAGGAATTGTTCTCGACCTCACCAACAACTTCAGTGATACTTCTACTTGTACTGCCATAGCTTCAGCTGGCTCTTCCTCCCATTCATCTGCACCGCTGGGGTTTAACAATTGGATGGGTGGCAAACCTGCAACTTATCACCATAATGCTGGTAATAGTACGATGATGATGGGCAACCATCATCTGCTGAGTAACAGCAGGAGGACATCTGGGGAAGAAATTATGACGTCGTCGTCAGCATTAATGGCTGAAAATGTGAGTGCTATTGCTTCAGATCCTAAATTTAGGGTTGCTGTTGCAGCTGCTATTACATCTCTCATTAATAGTAAAGAGAATAATACAACTCATCATCCTACTCCTCCTCATAATAATAATAATTTTGGTCCAAGGGATAATGGAGAGAAAGGTGGTAGCTAA

Protein Analysis

557

Amino Acids

61.65

Weight (kDa)

5.57

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 269 - 327 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AasI GACNNNNNNGTC 1 cut(s) 1486
AatII GACGTC 1 cut(s) 1484
Acc36I ACCTGC 1 cut(s) 1390
AccII CGCG 1 cut(s) 845
AciI CCGC 4 cut(s) 215, 712, 744, 1349
AclWI GGATC 6 cut(s) 14, 434, 447, 482, 630, 1523
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 4 cut(s) 404, 614, 1219, 1535
AcuI CTGAAG 3 cut(s) 1270, 1302, 1509
AcvI CACGTG 1 cut(s) 554
AcyI GRCGYC 1 cut(s) 1481
AfaI GTAC 3 cut(s) 252, 1306, 1414
AfiI CCNNNNNNNGG 2 cut(s) 436, 1256
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 5 cut(s) 200, 286, 398, 1053, 1254
AjnI CCWGG 1 cut(s) 892
Alw26I GTCTC 2 cut(s) 251, 686
AlwI GGATC 6 cut(s) 14, 434, 447, 482, 630, 1523
AlwNI CAGNNNCTG 2 cut(s) 1019, 1028
AoxI GGCC 1 cut(s) 723
ApeKI GCWGC 8 cut(s) 161, 706, 709, 1022, 1025, 1028, 1553, 1556
ApoI RAATTY 4 cut(s) 404, 614, 1219, 1535
ArsI GACNNNNNNTTYG 4 cut(s) 102, 134, 250, 282
AseI ATTAAT 2 cut(s) 1496, 1575
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 3 cut(s) 714, 916, 1637
AsuHPI GGTGA 8 cut(s) 56, 135, 236, 531, 537, 1136, 1267, 1385
AvaII GGWCC 3 cut(s) 714, 916, 1637
BamHI GGATCC 1 cut(s) 439
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BauI CACGAG 1 cut(s) 122
BbrPI CACGTG 1 cut(s) 554
BbsI GAAGAC 3 cut(s) 117, 293, 999
BbvI GCAGC 8 cut(s) 173, 693, 721, 1015, 1034, 1037, 1543, 1565
BccI CCATC 8 cut(s) 426, 550, 803, 1067, 1071, 1363, 1417, 1440
BciT130I CCWGG 1 cut(s) 894
BciVI GTATCC 1 cut(s) 121
BclI TGATCA 3 cut(s) 22, 61, 472
BcoDI GTCTC 2 cut(s) 251, 686
BfaI CTAG 5 cut(s) 584, 735, 765, 929, 1046
BfmI CTRYAG 3 cut(s) 219, 707, 1020
BfuAI ACCTGC 1 cut(s) 1390
BfuI GTATCC 1 cut(s) 121
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 894
Bme18I GGWCC 3 cut(s) 714, 916, 1637
BmgT120I GGNCC 3 cut(s) 714, 916, 1637
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 894
BmsI GCATC 2 cut(s) 229, 914
BpiI GAAGAC 3 cut(s) 117, 293, 999
BpmI CTGGAG 1 cut(s) 275
BsaAI YACGTR 1 cut(s) 554
BsaBI GATNNNNATC 2 cut(s) 541, 790
BsaHI GRCGYC 1 cut(s) 1481
BsaJI CCNNGG 2 cut(s) 435, 1640
BsaXI ACNNNNNCTCC 2 cut(s) 874, 904
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 1256
Bse118I RCCGGY 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 258, 986
Bse8I GATNNNNATC 2 cut(s) 541, 790
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 2 cut(s) 435, 1640
BseGI GGATG 6 cut(s) 238, 565, 902, 976, 1374, 1603
BseJI GATNNNNATC 2 cut(s) 541, 790
BseLI CCNNNNNNNGG 2 cut(s) 436, 1256
BseMII CTCAG 5 cut(s) 258, 458, 587, 1083, 1433
BseNI ACTGG 2 cut(s) 258, 986
BseRI GAGGAG 4 cut(s) 1078, 1186, 1602, 1605
BseXI GCAGC 8 cut(s) 173, 693, 721, 1015, 1034, 1037, 1543, 1565
BseYI CCCAGC 1 cut(s) 1351
BsgI GTGCAG 1 cut(s) 1329
Bsh1236I CGCG 1 cut(s) 845
BshFI GGCC 1 cut(s) 725
BsiSI CCGG 2 cut(s) 548, 726
BslI CCNNNNNNNGG 2 cut(s) 436, 1256
BsmAI GTCTC 2 cut(s) 251, 686
BsnI GGCC 1 cut(s) 725
Bsp68I TCGCGA 1 cut(s) 845
BspACI CCGC 4 cut(s) 215, 712, 744, 1349
BspANI GGCC 1 cut(s) 725
BspCNI CTCAG 5 cut(s) 257, 457, 588, 1082, 1434
BspFNI CGCG 1 cut(s) 845
BspHI TCATGA 4 cut(s) 475, 637, 1098, 1243
BspLI GGNNCC 1 cut(s) 441
BspMAI CTGCAG 2 cut(s) 711, 1024
BspMI ACCTGC 1 cut(s) 1390
BspPI GGATC 6 cut(s) 14, 434, 447, 482, 630, 1523
BspQI GCTCTTC 1 cut(s) 1333
BsrFI RCCGGY 1 cut(s) 547
BsrI ACTGG 2 cut(s) 258, 986
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 2 cut(s) 435, 1640
BssNI GRCGYC 1 cut(s) 1481
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 2 cut(s) 435, 1640
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 2 cut(s) 878, 886
Bst6I CTCTTC 2 cut(s) 171, 1333
BstACI GRCGYC 1 cut(s) 1481
BstAPI GCANNNNNTGC 1 cut(s) 1028
BstBAI YACGTR 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 1324
BstDEI CTNAG 5 cut(s) 244, 444, 596, 1069, 1442
BstEII GGTNACC 1 cut(s) 1153
BstENI CCTNNNNNAGG 1 cut(s) 1254
BstF5I GGATG 6 cut(s) 238, 565, 902, 976, 1374, 1603
BstFNI CGCG 1 cut(s) 845
BstHHI GCGC 1 cut(s) 308
BstMAI GTCTC 2 cut(s) 251, 686
BstMWI GCNNNNNNNGC 7 cut(s) 161, 703, 749, 1022, 1028, 1499, 1553
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 1 cut(s) 940
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 1 cut(s) 892
BstSFI CTRYAG 3 cut(s) 219, 707, 1020
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 8 cut(s) 173, 693, 721, 1015, 1034, 1037, 1543, 1565
BstV2I GAAGAC 3 cut(s) 117, 293, 999
BstX2I RGATCY 2 cut(s) 439, 1528
BstXI CCANNNNNNTGG 1 cut(s) 1403
BstYI RGATCY 2 cut(s) 439, 1528
BsuI GTATCC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 725
BtsCI GGATG 6 cut(s) 238, 565, 902, 976, 1374, 1603
BtsIMutI CAGTG 4 cut(s) 50, 593, 699, 1294
BtuMI TCGCGA 1 cut(s) 845
BveI ACCTGC 1 cut(s) 1390
Cac8I GCNNGC 1 cut(s) 1324
CaiI CAGNNNCTG 2 cut(s) 1019, 1028
CciI TCATGA 4 cut(s) 475, 637, 1098, 1243
CfoI GCGC 1 cut(s) 308
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 3 cut(s) 714, 916, 1637
CpoI CGGWCCG 1 cut(s) 714
Csp6I GTAC 3 cut(s) 251, 1305, 1413
CspI CGGWCCG 1 cut(s) 714
CviQI GTAC 3 cut(s) 251, 1305, 1413
DdeI CTNAG 5 cut(s) 244, 444, 596, 1069, 1442
DrdI GACNNNNNNGTC 1 cut(s) 1486
DseDI GACNNNNNNGTC 1 cut(s) 1486
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 2 cut(s) 171, 1333
EarI CTCTTC 2 cut(s) 171, 1333
Eco130I CCWWGG 2 cut(s) 435, 1640
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 3 cut(s) 714, 916, 1637
Eco57I CTGAAG 3 cut(s) 1270, 1302, 1509
Eco72I CACGTG 1 cut(s) 554
Eco91I GGTNACC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 1254
EcoO65I GGTNACC 1 cut(s) 1153
EcoRII CCWGG 1 cut(s) 892
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 435, 1640
ErhI CCWWGG 2 cut(s) 435, 1640
FbaI TGATCA 3 cut(s) 22, 61, 472
FokI GGATG 6 cut(s) 245, 572, 909, 963, 1381, 1590
FspBI CTAG 5 cut(s) 584, 735, 765, 929, 1046
GlaI GCGC 1 cut(s) 307
GsaI CCCAGC 1 cut(s) 1355
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 725
HapII CCGG 2 cut(s) 548, 726
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 1481
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HincII GTYRAC 1 cut(s) 581
HindII GTYRAC 1 cut(s) 581
HinfI GANTC 5 cut(s) 140, 325, 577, 791, 1049
HpaII CCGG 2 cut(s) 548, 726
HphI GGTGA 8 cut(s) 56, 135, 236, 531, 537, 1136, 1267, 1385
Hpy166II GTNNAC 2 cut(s) 121, 581
Hpy188I TCNGA 7 cut(s) 27, 447, 520, 627, 1072, 1211, 1528
Hpy188III TCNNGA 7 cut(s) 296, 476, 638, 844, 1099, 1244, 1268
Hpy8I GTNNAC 2 cut(s) 121, 581
Hpy99I CGWCG 5 cut(s) 107, 110, 531, 1486, 1489
HpyAV CCTTC 4 cut(s) 352, 373, 956, 1260
HpyCH4III ACNGT 2 cut(s) 878, 886
HpyCH4IV ACGT 4 cut(s) 499, 553, 652, 1481
HpyF10VI GCNNNNNNNGC 7 cut(s) 161, 703, 749, 1022, 1028, 1499, 1553
HpyF3I CTNAG 5 cut(s) 244, 444, 596, 1069, 1442
HpySE526I ACGT 4 cut(s) 499, 553, 652, 1481
Hsp92I GRCGYC 1 cut(s) 1481
HspAI GCGC 1 cut(s) 306
Ksp22I TGATCA 3 cut(s) 22, 61, 472
LguI GCTCTTC 1 cut(s) 1333
LmnI GCTCC 3 cut(s) 152, 367, 895
Lsp1109I GCAGC 8 cut(s) 173, 693, 721, 1015, 1034, 1037, 1543, 1565
LweI GCATC 2 cut(s) 229, 914
MaeI CTAG 5 cut(s) 584, 735, 765, 929, 1046
MaeII ACGT 4 cut(s) 499, 553, 652, 1481
MaeIII GTNAC 5 cut(s) 591, 653, 1041, 1153, 1445
MfeI CAATTG 1 cut(s) 1363
MflI RGATCY 2 cut(s) 439, 1528
MlyI GAGTC 2 cut(s) 334, 586
MseI TTAA 3 cut(s) 1359, 1496, 1575
MslI CAYNNNNRTG 1 cut(s) 1398
MspA1I CMGCKG 6 cut(s) 413, 712, 1028, 1322, 1351, 1556
MspI CCGG 2 cut(s) 548, 726
MspR9I CCNGG 1 cut(s) 894
MunI CAATTG 1 cut(s) 1363
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 1 cut(s) 845
MwoI GCNNNNNNNGC 7 cut(s) 161, 703, 749, 1022, 1028, 1499, 1553
NlaIV GGNNCC 1 cut(s) 441
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 1 cut(s) 591
NruI TCGCGA 1 cut(s) 845
NspI RCATGY 1 cut(s) 940
PagI TCATGA 4 cut(s) 475, 637, 1098, 1243
PciI ACATGT 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 1333
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 120, 129
PfeI GAWTC 3 cut(s) 140, 791, 1049
PflFI GACNNNGTC 2 cut(s) 116, 1483
PfoI TCCNGGA 1 cut(s) 892
PleI GAGTC 2 cut(s) 333, 585
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 2 cut(s) 333, 585
Ppu21I YACGTR 1 cut(s) 554
PscI ACATGT 1 cut(s) 936
PshBI ATTAAT 2 cut(s) 1496, 1575
PsiI TTATAA 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 892
PspCI CACGTG 1 cut(s) 554
PspEI GGTNACC 1 cut(s) 1153
PspFI CCCAGC 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 3 cut(s) 714, 916, 1637
PstI CTGCAG 2 cut(s) 711, 1024
PstNI CAGNNNCTG 2 cut(s) 1019, 1028
PsuI RGATCY 2 cut(s) 439, 1528
PsyI GACNNNGTC 2 cut(s) 116, 1483
PvuII CAGCTG 4 cut(s) 413, 1028, 1322, 1556
RruI TCGCGA 1 cut(s) 845
RsaI GTAC 3 cut(s) 252, 1306, 1414
RsaNI GTAC 3 cut(s) 251, 1305, 1413
RseI CAYNNNNRTG 1 cut(s) 1398
Rsr2I CGGWCCG 1 cut(s) 714
RsrII CGGWCCG 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 1333
SaqAI TTAA 3 cut(s) 1359, 1496, 1575
Sau96I GGNCC 3 cut(s) 714, 916, 1637
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 2 cut(s) 334, 586
ScrFI CCNGG 1 cut(s) 894
SfaNI GCATC 2 cut(s) 229, 914
SfcI CTRYAG 3 cut(s) 219, 707, 1020
SinI GGWCC 3 cut(s) 714, 916, 1637
SmiMI CAYNNNNRTG 1 cut(s) 1398
SsiI CCGC 4 cut(s) 215, 712, 744, 1349
SspI AATATT 2 cut(s) 196, 1190
SspMI CTAG 5 cut(s) 584, 735, 765, 929, 1046
StyD4I CCNGG 1 cut(s) 892
StyI CCWWGG 2 cut(s) 435, 1640
TaaI ACNGT 2 cut(s) 878, 886
TaiI ACGT 4 cut(s) 502, 556, 655, 1484
TaqI TCGA 1 cut(s) 1269
TaqII GACCGA 1 cut(s) 731
TatI WGTACW 2 cut(s) 250, 1304
TauI GCSGC 2 cut(s) 218, 746
TfiI GAWTC 3 cut(s) 140, 791, 1049
Tru1I TTAA 3 cut(s) 1359, 1496, 1575
Tru9I TTAA 3 cut(s) 1359, 1496, 1575
TscAI CASTG 4 cut(s) 57, 600, 699, 1294
TseFI GTSAC 1 cut(s) 591
TseI GCWGC 8 cut(s) 161, 706, 709, 1022, 1025, 1028, 1553, 1556
Tsp45I GTSAC 1 cut(s) 591
TspDTI ATGAA 9 cut(s) 347, 392, 416, 495, 627, 818, 1087, 1217, 1329
TspGWI ACGGA 1 cut(s) 1213
TspRI CASTG 4 cut(s) 57, 600, 699, 1294
Tth111I GACNNNGTC 2 cut(s) 116, 1483
VpaK11BI GGWCC 3 cut(s) 714, 916, 1637
VspI ATTAAT 2 cut(s) 1496, 1575
XagI CCTNNNNNAGG 1 cut(s) 1254
XapI RAATTY 4 cut(s) 404, 614, 1219, 1535
XceI RCATGY 1 cut(s) 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 1070
XspI CTAG 5 cut(s) 584, 735, 765, 929, 1046
ZraI GACGTC 1 cut(s) 1482
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.