Prupe.1G564300_v2.0.a1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011207
Physical Location & Seq
Forward (+)
46096258 .. 46098769
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G564300.1

Sequence Viewer

Length: 1686 bp
ATGGGGAGAGAGAAGGAGATGGGCTTGGATATTGATCTCTCACTCAAGATTGATTCTAAACAAGAAAAGCAAGGAGAAGACGGAGACCAAGGCGAAGGCGAAGAGGAAGAGGAAGAGAAAGAGGAAGAGGAAGATCTTAACGACGACGATGATATTGAACAAGGAGCACAACAAGAGGAGGATGAGCATGAACCAAGAGCACAGAATAACAACCTAAAAGCAGCAGAAGCCGCTGCTGGGGAAGTTGAAGATGATGCCTCAGTAGTAGAAACATCTTTGGAGGAGAATAACAAGATGAAGACGGATCAAGAGCTATGCGTTTTACAAATGGAGATGAGCCGCATGAAAGAAGAAAACAAAGTTTTAAGGAAGGTCGTGGAGCAAACCATGAAAGATTATTATGATCTCCAAATGAAATTTACGGCTCTCCACCAAAGCAACCAAAGCAAGGATCCTCAAACCTTTCTATCACTTGATGGTAATGATCCCGATGCTGTTCAAGGACCCAAGACGATTCCAAAGAGATCTCCATTATCACCAACACATGAAGATATGATGAAAGAGAGTCAACTAGGGTTGTCACTGAGGCTACAAACCAGCCCAACTACCAATATTCAATCACACGGAAGAGAAGAAGAAGATGATGATAAAGAAGAGAACAACAAGAAGGAAGAATTGACGCGTTCAAGTCTTGCATCATCACTACTGCAGAACAAGCTTCAGCGAACCGAATTGCCCGGAATTTCGAGTCACGTTGCTTCCCAACCCAACAGAAAAGCTAGGGTTTCGGTTAGAGCAAGATGTGAATCCGCCACAATGAATGATGGGTGTCAATGGAGGAAATACGGACAGAAAATTGCAAAAGGAAACCCTTGCCCACGAGCTTATTATCGTTGCACAGTAGCTCCTGGATGCCCAGTTAGGAAACAGGTCCAAAGATGCCTAGAGGACATGTCCATACTCATAACAACATATGAAGGAACACATAACCATCCACTTCCTGTGGGTGCAACAGCCATGGCTTCAACAGCTTCTGCAGCAGCTTCATTCATGTTATTAGATTCAAGTAACCATCATCTTTTAGATGGAACACCCTCTAATTACACCCAGGCATCCCTCCCTAATTACTATAATGTCCCTGATCACAGGATGAACCCTTCATCTCATTCCTCTTCAAACTTTAGAACCATCAATTCTCATGACCCTTCCAAAGGAATTGTTCTTGACCTCACAAACAACTTTTATGATCACAACAATACACCACAAAACATTCCCATGGGCAGCTCCTCATCAGCACCACCAGGTCTTAGTAACTGGATGATGAGCACAAGCAGATCTGCAAGTAGTTATCAAAACCCTAATTCTATAACCAGCCGTCTTTTGGCTAACAGCACTATTTCCAGATCATCAGGAGTTGGAGTGGAAGATCAGAGAAGTTGGAGAGGGGAAGAAAATAATATGTCATTGGCTGAGAATGTGACTGCCATTGCTTCAGATCCCAAATTTAGGGTTGCTGTTGCAGCTGCTATCACATCCCTCATTAATAAAGAGAATCATACAGCTAATCATCCTTTTGGTCCAAGGGATGGAGGAGAGAATGGTAGTTCTACTAGCAACAATTGGGCCCTTGATCACCAGTCCCTCTCAGCCAATGGTAAGCCCATTCGCAAAAACAGCTTAGAATAA

Protein Analysis

562

Amino Acids

62.07

Weight (kDa)

5.06

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1586
AccII CGCG 1 cut(s) 682
AciI CCGC 3 cut(s) 231, 340, 810
AclWI GGATC 5 cut(s) 312, 446, 459, 479, 1490
AcsI RAATTY 3 cut(s) 416, 741, 1502
AcuI CTGAAG 2 cut(s) 704, 1476
AfiI CCNNNNNNNGG 6 cut(s) 237, 448, 1211, 1381, 1506, 1586
AflIII ACRYGT 2 cut(s) 680, 951
AgsI TTSAA 8 cut(s) 158, 248, 500, 617, 687, 1026, 1065, 1176
AjnI CCWGG 3 cut(s) 907, 1107, 1300
Alw21I GWGCWC 3 cut(s) 169, 202, 1328
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 5 cut(s) 312, 446, 459, 479, 1490
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 1 cut(s) 1623
ApaI GGGCCC 1 cut(s) 1627
ApeKI GCWGC 7 cut(s) 221, 233, 1037, 1040, 1281, 1520, 1523
ApoI RAATTY 3 cut(s) 416, 741, 1502
AseI ATTAAT 1 cut(s) 1542
AspS9I GGNCC 5 cut(s) 503, 931, 1577, 1623, 1624
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 2 cut(s) 528, 1625
AvaII GGWCC 3 cut(s) 503, 931, 1577
BaeGI GKGCMC 1 cut(s) 1627
BamHI GGATCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 1627
BauI CACGAG 1 cut(s) 879
BbsI GAAGAC 2 cut(s) 84, 305
Bbv12I GWGCWC 3 cut(s) 169, 202, 1328
BbvI GCAGC 7 cut(s) 220, 233, 1049, 1052, 1293, 1510, 1532
BccI CCATC 8 cut(s) 13, 470, 818, 999, 1079, 1080, 1196, 1580
BceAI ACGGC 2 cut(s) 438, 1359
BciT130I CCWGG 3 cut(s) 909, 1109, 1302
BclI TGATCA 3 cut(s) 1141, 1246, 1630
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 4 cut(s) 572, 780, 944, 1611
BfmI CTRYAG 2 cut(s) 707, 1035
BglII AGATCT 3 cut(s) 133, 524, 1334
BisI GCNGC 9 cut(s) 222, 231, 234, 340, 1038, 1041, 1282, 1521, 1524
BlsI GCNGC 9 cut(s) 223, 232, 235, 341, 1039, 1042, 1283, 1522, 1525
Bme1390I CCNGG 4 cut(s) 738, 909, 1109, 1302
Bme18I GGWCC 3 cut(s) 503, 931, 1577
BmgT120I GGNCC 5 cut(s) 503, 931, 1577, 1623, 1624
BmiI GGNNCC 3 cut(s) 453, 505, 1625
BmrFI CCNGG 4 cut(s) 738, 909, 1109, 1302
BmrI ACTGGG 1 cut(s) 911
BmsI GCATC 6 cut(s) 244, 481, 704, 902, 929, 1121
BmuI ACTGGG 1 cut(s) 911
BpiI GAAGAC 2 cut(s) 84, 305
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 738
BsaBI GATNNNNATC 2 cut(s) 33, 805
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 88, 1017, 1107, 1275, 1580
BsaXI ACNNNNNCTCC 4 cut(s) 889, 919, 1404, 1434
Bsc4I CCNNNNNNNGG 6 cut(s) 237, 448, 1211, 1381, 1506, 1586
Bse1I ACTGG 3 cut(s) 917, 1319, 1636
Bse3DI GCAATG 1 cut(s) 1485
Bse8I GATNNNNATC 2 cut(s) 33, 805
BseBI CCWGG 3 cut(s) 909, 1109, 1302
BseDI CCNNGG 5 cut(s) 88, 1017, 1107, 1275, 1580
BseGI GGATG 9 cut(s) 187, 917, 991, 1112, 1155, 1323, 1532, 1567, 1591
BseJI GATNNNNATC 2 cut(s) 33, 805
BseLI CCNNNNNNNGG 6 cut(s) 237, 448, 1211, 1381, 1506, 1586
BseMI GCAATG 1 cut(s) 1485
BseMII CTCAG 4 cut(s) 273, 575, 1461, 1659
BseNI ACTGG 3 cut(s) 917, 1319, 1636
BseRI GAGGAG 4 cut(s) 191, 296, 1276, 1605
BseSI GKGCMC 1 cut(s) 1627
BseXI GCAGC 7 cut(s) 220, 233, 1049, 1052, 1293, 1510, 1532
BseYI CCCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 682
BshFI GGCC 1 cut(s) 1625
BsiHKAI GWGCWC 3 cut(s) 169, 202, 1328
BsiSI CCGG 1 cut(s) 738
BslFI GGGAC 2 cut(s) 1121, 1624
BslI CCNNNNNNNGG 6 cut(s) 237, 448, 1211, 1381, 1506, 1586
BsmAI GTCTC 1 cut(s) 78
BsmFI GGGAC 2 cut(s) 1121, 1624
BsnI GGCC 1 cut(s) 1625
Bso31I GGTCTC 1 cut(s) 78
Bsp120I GGGCCC 1 cut(s) 1623
Bsp1286I GDGCHC 4 cut(s) 169, 202, 1328, 1627
Bsp19I CCATGG 2 cut(s) 1017, 1275
BspACI CCGC 3 cut(s) 231, 340, 810
BspANI GGCC 1 cut(s) 1625
BspCNI CTCAG 4 cut(s) 272, 576, 1462, 1658
BspFNI CGCG 1 cut(s) 682
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 3 cut(s) 453, 505, 1625
BspMAI CTGCAG 2 cut(s) 711, 1039
BspPI GGATC 5 cut(s) 312, 446, 459, 479, 1490
BspTNI GGTCTC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 1485
BsrI ACTGG 3 cut(s) 917, 1319, 1636
BssECI CCNNGG 5 cut(s) 88, 1017, 1107, 1275, 1580
BssSI CACGAG 1 cut(s) 879
BssT1I CCWWGG 4 cut(s) 88, 1017, 1275, 1580
Bst2BI CACGAG 1 cut(s) 879
Bst2UI CCWGG 3 cut(s) 909, 1109, 1302
Bst4CI ACNGT 1 cut(s) 901
Bst6I CTCTTC 7 cut(s) 96, 102, 108, 120, 622, 648, 1177
BstDEI CTNAG 6 cut(s) 259, 584, 1307, 1470, 1645, 1678
BstDSI CCRYGG 2 cut(s) 1017, 1275
BstENI CCTNNNNNAGG 1 cut(s) 1209
BstF5I GGATG 9 cut(s) 187, 917, 991, 1112, 1155, 1323, 1532, 1567, 1591
BstFNI CGCG 1 cut(s) 682
BstMAI GTCTC 1 cut(s) 78
BstMWI GCNNNNNNNGC 8 cut(s) 227, 230, 444, 715, 1028, 1037, 1520, 1674
BstNI CCWGG 3 cut(s) 909, 1109, 1302
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 4 cut(s) 736, 907, 1107, 1300
BstSFI CTRYAG 2 cut(s) 707, 1035
BstSLI GKGCMC 1 cut(s) 1627
BstUI CGCG 1 cut(s) 682
BstV1I GCAGC 7 cut(s) 220, 233, 1049, 1052, 1293, 1510, 1532
BstV2I GAAGAC 2 cut(s) 84, 305
BstX2I RGATCY 5 cut(s) 133, 451, 524, 1334, 1495
BstYI RGATCY 5 cut(s) 133, 451, 524, 1334, 1495
BsuRI GGCC 1 cut(s) 1625
BtgI CCRYGG 2 cut(s) 1017, 1275
BtsCI GGATG 9 cut(s) 187, 917, 991, 1112, 1155, 1323, 1532, 1567, 1591
BtsIMutI CAGTG 1 cut(s) 581
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 1198
Cfr13I GGNCC 5 cut(s) 503, 931, 1577, 1623, 1624
CseI GACGC 1 cut(s) 688
CsiI ACCWGGT 1 cut(s) 1300
CviAII CATG 9 cut(s) 188, 343, 388, 545, 952, 1018, 1051, 1199, 1276
DdeI CTNAG 6 cut(s) 259, 584, 1307, 1470, 1645, 1678
Eam1104I CTCTTC 7 cut(s) 96, 102, 108, 120, 622, 648, 1177
EarI CTCTTC 7 cut(s) 96, 102, 108, 120, 622, 648, 1177
EciI GGCGGA 1 cut(s) 799
Eco130I CCWWGG 4 cut(s) 88, 1017, 1275, 1580
Eco24I GRGCYC 1 cut(s) 1627
Eco31I GGTCTC 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 503, 931, 1577
Eco57I CTGAAG 2 cut(s) 704, 1476
EcoNI CCTNNNNNAGG 1 cut(s) 1209
EcoO109I RGGNCCY 2 cut(s) 503, 1624
EcoRII CCWGG 3 cut(s) 907, 1107, 1300
EcoT14I CCWWGG 4 cut(s) 88, 1017, 1275, 1580
EcoT38I GRGCYC 1 cut(s) 1627
ErhI CCWWGG 4 cut(s) 88, 1017, 1275, 1580
FaeI CATG 9 cut(s) 191, 346, 391, 548, 955, 1021, 1054, 1202, 1279
FaqI GGGAC 2 cut(s) 1121, 1624
FatI CATG 9 cut(s) 187, 342, 387, 544, 951, 1017, 1050, 1198, 1275
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 3 cut(s) 1141, 1246, 1630
Fnu4HI GCNGC 9 cut(s) 222, 231, 234, 340, 1038, 1041, 1282, 1521, 1524
FokI GGATG 9 cut(s) 194, 924, 978, 1099, 1162, 1330, 1519, 1554, 1598
FriOI GRGCYC 1 cut(s) 1627
Fsp4HI GCNGC 9 cut(s) 222, 231, 234, 340, 1038, 1041, 1282, 1521, 1524
FspBI CTAG 4 cut(s) 572, 780, 944, 1611
GluI GCNGC 9 cut(s) 222, 231, 234, 340, 1038, 1041, 1282, 1521, 1524
GsaI CCCAGC 1 cut(s) 240
HaeIII GGCC 1 cut(s) 1625
HapII CCGG 1 cut(s) 738
HgaI GACGC 1 cut(s) 688
Hin1II CATG 9 cut(s) 191, 346, 391, 548, 955, 1021, 1054, 1202, 1279
HincII GTYRAC 1 cut(s) 569
HindII GTYRAC 1 cut(s) 569
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 7 cut(s) 53, 514, 565, 748, 806, 1061, 1552
HpaII CCGG 1 cut(s) 738
HphI GGTGA 2 cut(s) 528, 1625
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 2 cut(s) 1431, 1495
Hpy188III TCNNGA 7 cut(s) 46, 308, 488, 1199, 1223, 1401, 1410
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 2 cut(s) 146, 149
HpyAV CCTTC 7 cut(s) 7, 89, 364, 661, 971, 1167, 1215
HpyCH4III ACNGT 1 cut(s) 901
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 8 cut(s) 695, 709, 860, 897, 1010, 1037, 1340, 1520
HpyF10VI GCNNNNNNNGC 8 cut(s) 227, 230, 444, 715, 1028, 1037, 1520, 1674
HpyF3I CTNAG 6 cut(s) 259, 584, 1307, 1470, 1645, 1678
HpySE526I ACGT 1 cut(s) 753
Hsp92II CATG 9 cut(s) 191, 346, 391, 548, 955, 1021, 1054, 1202, 1279
Ksp22I TGATCA 3 cut(s) 1141, 1246, 1630
LmnI GCTCC 4 cut(s) 164, 379, 910, 1289
Lsp1109I GCAGC 7 cut(s) 220, 233, 1049, 1052, 1293, 1510, 1532
LweI GCATC 6 cut(s) 244, 481, 704, 902, 929, 1121
MabI ACCWGGT 1 cut(s) 1300
MaeI CTAG 4 cut(s) 572, 780, 944, 1611
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 5 cut(s) 579, 749, 1067, 1310, 1477
MfeI CAATTG 1 cut(s) 1618
MflI RGATCY 5 cut(s) 133, 451, 524, 1334, 1495
MhlI GDGCHC 4 cut(s) 169, 202, 1328, 1627
MluI ACGCGT 1 cut(s) 680
MlyI GAGTC 2 cut(s) 574, 757
MmeI TCCRAC 2 cut(s) 1396, 1418
MseI TTAA 3 cut(s) 138, 365, 1542
MslI CAYNNNNRTG 1 cut(s) 1274
MspA1I CMGCKG 2 cut(s) 233, 1523
MspI CCGG 1 cut(s) 738
MspR9I CCNGG 4 cut(s) 738, 909, 1109, 1302
MunI CAATTG 1 cut(s) 1618
MvaI CCWGG 3 cut(s) 909, 1109, 1302
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 8 cut(s) 227, 230, 444, 715, 1028, 1037, 1520, 1674
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 2 cut(s) 1017, 1275
NdeI CATATG 1 cut(s) 973
NlaIII CATG 9 cut(s) 191, 346, 391, 548, 955, 1021, 1054, 1202, 1279
NlaIV GGNNCC 3 cut(s) 453, 505, 1625
NmuCI GTSAC 3 cut(s) 579, 749, 1477
NspI RCATGY 1 cut(s) 955
PagI TCATGA 1 cut(s) 1198
PciI ACATGT 1 cut(s) 951
PfeI GAWTC 5 cut(s) 53, 514, 806, 1061, 1552
PflMI CCANNNNNTGG 1 cut(s) 1586
PfoI TCCNGGA 1 cut(s) 907
PkrI GCNGC 9 cut(s) 223, 232, 235, 341, 1039, 1042, 1283, 1522, 1525
PleI GAGTC 2 cut(s) 573, 756
PpsI GAGTC 2 cut(s) 573, 756
PpuMI RGGWCCY 1 cut(s) 503
PscI ACATGT 1 cut(s) 951
PshBI ATTAAT 1 cut(s) 1542
Psp5II RGGWCCY 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 907, 1107, 1300
PspFI CCCAGC 1 cut(s) 236
PspGI CCWGG 3 cut(s) 907, 1107, 1300
PspN4I GGNNCC 3 cut(s) 453, 505, 1625
PspOMI GGGCCC 1 cut(s) 1623
PspPI GGNCC 5 cut(s) 503, 931, 1577, 1623, 1624
PspPPI RGGWCCY 1 cut(s) 503
PstI CTGCAG 2 cut(s) 711, 1039
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 5 cut(s) 133, 451, 524, 1334, 1495
PvuII CAGCTG 1 cut(s) 1523
RseI CAYNNNNRTG 1 cut(s) 1274
SaqAI TTAA 3 cut(s) 138, 365, 1542
SatI GCNGC 9 cut(s) 222, 231, 234, 340, 1038, 1041, 1282, 1521, 1524
Sau96I GGNCC 5 cut(s) 503, 931, 1577, 1623, 1624
SchI GAGTC 2 cut(s) 574, 757
ScrFI CCNGG 4 cut(s) 738, 909, 1109, 1302
SduI GDGCHC 4 cut(s) 169, 202, 1328, 1627
SexAI ACCWGGT 1 cut(s) 1300
SfaNI GCATC 6 cut(s) 244, 481, 704, 902, 929, 1121
SfcI CTRYAG 2 cut(s) 707, 1035
SinI GGWCC 3 cut(s) 503, 931, 1577
SmiMI CAYNNNNRTG 1 cut(s) 1274
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SsiI CCGC 3 cut(s) 231, 340, 810
SspI AATATT 1 cut(s) 613
SspMI CTAG 4 cut(s) 572, 780, 944, 1611
StyD4I CCNGG 4 cut(s) 736, 907, 1107, 1300
StyI CCWWGG 4 cut(s) 88, 1017, 1275, 1580
TaaI ACNGT 1 cut(s) 901
TaiI ACGT 1 cut(s) 756
TaqI TCGA 1 cut(s) 746
TauI GCSGC 2 cut(s) 233, 342
TfiI GAWTC 5 cut(s) 53, 514, 806, 1061, 1552
Tru1I TTAA 3 cut(s) 138, 365, 1542
Tru9I TTAA 3 cut(s) 138, 365, 1542
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 3 cut(s) 579, 749, 1477
TseI GCWGC 7 cut(s) 221, 233, 1037, 1040, 1281, 1520, 1523
Tsp45I GTSAC 3 cut(s) 579, 749, 1477
TspGWI ACGGA 4 cut(s) 96, 317, 639, 861
TspRI CASTG 1 cut(s) 588
Van91I CCANNNNNTGG 1 cut(s) 1586
VpaK11BI GGWCC 3 cut(s) 503, 931, 1577
VspI ATTAAT 1 cut(s) 1542
XagI CCTNNNNNAGG 1 cut(s) 1209
XapI RAATTY 3 cut(s) 416, 741, 1502
XceI RCATGY 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 1378
XspI CTAG 4 cut(s) 572, 780, 944, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.