RchiOBHm_Chr7g0223361
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011207
Physical Location & Seq
Reverse (-)
45049165 .. 45051484
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19998

Sequence Viewer

Length: 1374 bp
ATGGAGATGAGTCGCATGAAAGAAGAAAATAAGGTTTTGAGGAAGGTCGTGGAGCAAACCATGAAGGATTATTACGATCTTCAAATGAAATTCACAGCTGTCCATCAAAATAGCCAAACCAAGGATCCTCAGACCTTTTTATCGCTTCGTGGTAATGATCATGATGAAGCTGATCCTCCAAAAAGTAGTATTCCCAAGATGTCGGAAATTCGTCGTCATAGATCTCCATCACCGGCACGTGCGGATGATATCATAAAAGAGAGTCAACTAGGGCTGTCACTGAGGCTACAAACCAATGAATTTAGGGATCAAAGAGAAGATCACGATATCCAAGAACGTAACAAGGAAGAATTGACGAGTAGTAGTCTCACATCATCAGTGCAACACAAGCTGCAGCGGACCGATTTGGCCGGAATTACTAGCCATGCCGCCCAAGCCAACAGAAAAGCTAGGGTTTCGGTTAGAGCAAGATGTGAATCTGCCACAATGAATGATGGGTGTCAATGGAGGAAATATGGGCAGAAAATCGCGAAAGGAAACCCTTGCCCCAGAGCCTATTACCGTTGCACAGTAGCTCCTGGATGTCCTGTTAGAAAACAGGTCCAAAGATGCCTAGAGGACATGTCCATACTGATAACAACATACGAAGGAACACATAATCATCCACTTCCAGTTGGTGCAACTGCCATGGCTTCCACAGCTTCTGCAGCAGCTGCTTCCTTTATGTTACTAGATTCAAGCAACCACCATCTCTCAGATGGAACACTACCCTCCTCTAATTTCATGACCCAAGCCTCCCTCCCTCCCAATAATTACTACAACACCCCTCACCACATGTTAAACAACCACTACCCCTCATCAATATCTCATCAATATTCCTCCTCATCAAACTTCCGAACCCTAAATTCCGTTCATCATCAGCCTCATCATGACCCTTCAAAAGGAATCGTTCTCGACCTCACCAACAACTTCAGTGATACTTCTACTAGTACTGCCATAGCTTCAGCTGGCTCTTCCTCCCATTCATCTGCACCGCTGGGGTTTAACAATTGGATGGGTGGCAAACCTGCAAATTATCACCATAATGCTGGTAATAGTACGATGATAATGGGCAACCATCATCTGCTGAGTAACAGCAGGAGGACATCTGGGGAAGAAAGTATGACGTCGTCGTCAGCATTAATGGCTGAAAATGTGAGTGCTATTGCTTCAGATCCTAAATTTAGGGTTGCTGTTGCCGCTGCTATTACATCTCTCATTAATAGCAAAGAGAATAACACAACTCATCATCCTACTCCTCCTCATAATAATAATAATAATAATAATAATAATAATTTTGGTCCAAGGGATAATGGAGAGAAAGGTGGTAGCTAA

Protein Analysis

457

Amino Acids

50.41

Weight (kDa)

8.9

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 164 - 222 9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1171
AatII GACGTC 1 cut(s) 1169
Acc36I ACCTGC 1 cut(s) 1075
AccII CGCG 1 cut(s) 530
AciI CCGC 5 cut(s) 242, 397, 429, 1034, 1239
AclWI GGATC 5 cut(s) 119, 132, 167, 315, 1208
AcoI YGGCCR 1 cut(s) 408
AcsI RAATTY 5 cut(s) 89, 207, 299, 904, 1220
AcuI CTGAAG 3 cut(s) 955, 987, 1194
AcvI CACGTG 1 cut(s) 239
AcyI GRCGYC 1 cut(s) 1166
AfaI GTAC 2 cut(s) 991, 1099
AfiI CCNNNNNNNGG 2 cut(s) 121, 941
AflIII ACRYGT 2 cut(s) 621, 834
AgsI TTSAA 3 cut(s) 83, 738, 939
AhlI ACTAGT 1 cut(s) 986
AjnI CCWGG 1 cut(s) 577
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 5 cut(s) 119, 132, 167, 315, 1208
AlwNI CAGNNNCTG 2 cut(s) 704, 713
AoxI GGCC 1 cut(s) 408
ApeKI GCWGC 6 cut(s) 391, 394, 707, 710, 713, 1241
ApoI RAATTY 5 cut(s) 89, 207, 299, 904, 1220
AseI ATTAAT 2 cut(s) 1181, 1260
AspS9I GGNCC 3 cut(s) 399, 601, 1340
AsuHPI GGTGA 4 cut(s) 222, 821, 952, 1070
AvaII GGWCC 3 cut(s) 399, 601, 1340
BamHI GGATCC 1 cut(s) 124
BarI GAAGNNNNNNTAC 2 cut(s) 56, 88
BbrPI CACGTG 1 cut(s) 239
BbvI GCAGC 6 cut(s) 378, 406, 700, 719, 722, 1228
BccI CCATC 7 cut(s) 111, 235, 488, 752, 756, 1048, 1125
BciT130I CCWGG 1 cut(s) 579
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 371
BcuI ACTAGT 1 cut(s) 986
BfaI CTAG 6 cut(s) 269, 420, 450, 614, 731, 987
BfmI CTRYAG 2 cut(s) 392, 705
BfuAI ACCTGC 1 cut(s) 1075
BglII AGATCT 1 cut(s) 221
BisI GCNGC 8 cut(s) 392, 395, 429, 708, 711, 714, 1239, 1242
BlsI GCNGC 8 cut(s) 393, 396, 430, 709, 712, 715, 1240, 1243
BmcAI AGTACT 1 cut(s) 991
Bme1390I CCNGG 1 cut(s) 579
Bme18I GGWCC 3 cut(s) 399, 601, 1340
BmgT120I GGNCC 3 cut(s) 399, 601, 1340
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 1 cut(s) 579
BmsI GCATC 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 239
BsaBI GATNNNNATC 2 cut(s) 226, 475
BsaHI GRCGYC 1 cut(s) 1166
BsaJI CCNNGG 3 cut(s) 120, 687, 1343
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 941
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 671
Bse8I GATNNNNATC 2 cut(s) 226, 475
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 3 cut(s) 120, 687, 1343
BseGI GGATG 5 cut(s) 250, 587, 661, 1059, 1288
BseJI GATNNNNATC 2 cut(s) 226, 475
BseLI CCNNNNNNNGG 2 cut(s) 121, 941
BseMII CTCAG 4 cut(s) 143, 272, 768, 1118
BseNI ACTGG 1 cut(s) 671
BseRI GAGGAG 4 cut(s) 763, 871, 1287, 1290
BseXI GCAGC 6 cut(s) 378, 406, 700, 719, 722, 1228
BseYI CCCAGC 1 cut(s) 1036
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 410
BsiSI CCGG 2 cut(s) 233, 411
BslI CCNNNNNNNGG 2 cut(s) 121, 941
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 1 cut(s) 410
Bsp143I GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
Bsp19I CCATGG 1 cut(s) 687
Bsp68I TCGCGA 1 cut(s) 530
BspACI CCGC 5 cut(s) 242, 397, 429, 1034, 1239
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 4 cut(s) 142, 273, 767, 1119
BspFNI CGCG 1 cut(s) 530
BspHI TCATGA 3 cut(s) 160, 783, 928
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 2 cut(s) 396, 709
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 5 cut(s) 119, 132, 167, 315, 1208
BspQI GCTCTTC 1 cut(s) 1018
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 1 cut(s) 671
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 3 cut(s) 120, 687, 1343
BssMI GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
BssNI GRCGYC 1 cut(s) 1166
BssT1I CCWWGG 3 cut(s) 120, 687, 1343
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 563, 571
Bst6I CTCTTC 1 cut(s) 1018
BstACI GRCGYC 1 cut(s) 1166
BstAPI GCANNNNNTGC 1 cut(s) 713
BstBAI YACGTR 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 1009
BstDEI CTNAG 4 cut(s) 129, 281, 754, 1127
BstDSI CCRYGG 1 cut(s) 687
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 5 cut(s) 250, 587, 661, 1059, 1288
BstFNI CGCG 1 cut(s) 530
BstKTI GATC 8 cut(s) 79, 127, 160, 175, 224, 310, 322, 1216
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
BstMWI GCNNNNNNNGC 7 cut(s) 388, 434, 698, 707, 713, 1184, 1238
BstNI CCWGG 1 cut(s) 579
BstNSI RCATGY 2 cut(s) 625, 838
BstSCI CCNGG 1 cut(s) 577
BstSFI CTRYAG 2 cut(s) 392, 705
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 6 cut(s) 378, 406, 700, 719, 722, 1228
BstX2I RGATCY 3 cut(s) 124, 221, 1213
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 3 cut(s) 124, 221, 1213
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 5 cut(s) 250, 587, 661, 1059, 1288
BtsIMutI CAGTG 3 cut(s) 278, 384, 979
BtuMI TCGCGA 1 cut(s) 530
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 1 cut(s) 1009
CaiI CAGNNNCTG 2 cut(s) 704, 713
CciI TCATGA 3 cut(s) 160, 783, 928
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 399, 601, 1340
CpoI CGGWCCG 1 cut(s) 399
Csp6I GTAC 2 cut(s) 990, 1098
CspI CGGWCCG 1 cut(s) 399
CviAII CATG 9 cut(s) 16, 61, 161, 425, 622, 688, 784, 835, 929
CviQI GTAC 2 cut(s) 990, 1098
DdeI CTNAG 4 cut(s) 129, 281, 754, 1127
DpnI GATC 8 cut(s) 78, 126, 159, 174, 223, 309, 321, 1215
DpnII GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
DrdI GACNNNNNNGTC 1 cut(s) 1171
DseDI GACNNNNNNGTC 1 cut(s) 1171
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 1018
EarI CTCTTC 1 cut(s) 1018
Eco130I CCWWGG 3 cut(s) 120, 687, 1343
Eco32I GATATC 2 cut(s) 250, 328
Eco47I GGWCC 3 cut(s) 399, 601, 1340
Eco57I CTGAAG 3 cut(s) 955, 987, 1194
Eco72I CACGTG 1 cut(s) 239
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoRII CCWGG 1 cut(s) 577
EcoRV GATATC 2 cut(s) 250, 328
EcoT14I CCWWGG 3 cut(s) 120, 687, 1343
ErhI CCWWGG 3 cut(s) 120, 687, 1343
FaeI CATG 9 cut(s) 19, 64, 164, 428, 625, 691, 787, 838, 932
FatI CATG 9 cut(s) 15, 60, 160, 424, 621, 687, 783, 834, 928
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 8 cut(s) 392, 395, 429, 708, 711, 714, 1239, 1242
FokI GGATG 5 cut(s) 257, 594, 648, 1066, 1275
Fsp4HI GCNGC 8 cut(s) 392, 395, 429, 708, 711, 714, 1239, 1242
FspBI CTAG 6 cut(s) 269, 420, 450, 614, 731, 987
GluI GCNGC 8 cut(s) 392, 395, 429, 708, 711, 714, 1239, 1242
GsaI CCCAGC 1 cut(s) 1040
HaeIII GGCC 1 cut(s) 410
HapII CCGG 2 cut(s) 233, 411
Hin1I GRCGYC 1 cut(s) 1166
Hin1II CATG 9 cut(s) 19, 64, 164, 428, 625, 691, 787, 838, 932
HincII GTYRAC 1 cut(s) 266
HindII GTYRAC 1 cut(s) 266
HinfI GANTC 5 cut(s) 10, 262, 476, 734, 945
HpaII CCGG 2 cut(s) 233, 411
HphI GGTGA 4 cut(s) 222, 821, 952, 1070
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 132, 205, 757, 896, 1213
Hpy188III TCNNGA 6 cut(s) 161, 323, 529, 784, 929, 953
Hpy8I GTNNAC 1 cut(s) 266
Hpy99I CGWCG 3 cut(s) 216, 1171, 1174
HpyAV CCTTC 4 cut(s) 37, 58, 641, 945
HpyCH4III ACNGT 2 cut(s) 563, 571
HpyCH4IV ACGT 3 cut(s) 238, 337, 1166
HpyCH4V TGCA 7 cut(s) 382, 394, 567, 680, 707, 1031, 1070
HpyF10VI GCNNNNNNNGC 7 cut(s) 388, 434, 698, 707, 713, 1184, 1238
HpyF3I CTNAG 4 cut(s) 129, 281, 754, 1127
HpySE526I ACGT 3 cut(s) 238, 337, 1166
Hsp92I GRCGYC 1 cut(s) 1166
Hsp92II CATG 9 cut(s) 19, 64, 164, 428, 625, 691, 787, 838, 932
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
LguI GCTCTTC 1 cut(s) 1018
LmnI GCTCC 2 cut(s) 52, 580
Lsp1109I GCAGC 6 cut(s) 378, 406, 700, 719, 722, 1228
LweI GCATC 1 cut(s) 599
MaeI CTAG 6 cut(s) 269, 420, 450, 614, 731, 987
MaeII ACGT 3 cut(s) 238, 337, 1166
MaeIII GTNAC 4 cut(s) 276, 338, 726, 1130
MalI GATC 8 cut(s) 78, 126, 159, 174, 223, 309, 321, 1215
MboI GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
MboII GAAGA 6 cut(s) 35, 71, 329, 359, 1005, 1166
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 3 cut(s) 124, 221, 1213
MlyI GAGTC 2 cut(s) 19, 271
MmeI TCCRAC 1 cut(s) 183
MseI TTAA 4 cut(s) 839, 1044, 1181, 1260
MslI CAYNNNNRTG 1 cut(s) 1083
MspA1I CMGCKG 6 cut(s) 98, 397, 713, 1007, 1036, 1241
MspI CCGG 2 cut(s) 233, 411
MspR9I CCNGG 1 cut(s) 579
MunI CAATTG 1 cut(s) 1048
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 7 cut(s) 388, 434, 698, 707, 713, 1184, 1238
NcoI CCATGG 1 cut(s) 687
NdeII GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
NlaIII CATG 9 cut(s) 19, 64, 164, 428, 625, 691, 787, 838, 932
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 276
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 2 cut(s) 625, 838
PagI TCATGA 3 cut(s) 160, 783, 928
PciI ACATGT 2 cut(s) 621, 834
PciSI GCTCTTC 1 cut(s) 1018
PfeI GAWTC 3 cut(s) 476, 734, 945
PflFI GACNNNGTC 1 cut(s) 1168
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 8 cut(s) 393, 396, 430, 709, 712, 715, 1240, 1243
PleI GAGTC 2 cut(s) 18, 270
PmaCI CACGTG 1 cut(s) 239
PmlI CACGTG 1 cut(s) 239
PpsI GAGTC 2 cut(s) 18, 270
Ppu21I YACGTR 1 cut(s) 239
PscI ACATGT 2 cut(s) 621, 834
PshBI ATTAAT 2 cut(s) 1181, 1260
Psp6I CCWGG 1 cut(s) 577
PspCI CACGTG 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 1036
PspGI CCWGG 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 3 cut(s) 399, 601, 1340
PstI CTGCAG 2 cut(s) 396, 709
PstNI CAGNNNCTG 2 cut(s) 704, 713
PsuI RGATCY 3 cut(s) 124, 221, 1213
PsyI GACNNNGTC 1 cut(s) 1168
PvuII CAGCTG 3 cut(s) 98, 713, 1007
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 2 cut(s) 991, 1099
RsaNI GTAC 2 cut(s) 990, 1098
RseI CAYNNNNRTG 1 cut(s) 1083
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 1018
SaqAI TTAA 4 cut(s) 839, 1044, 1181, 1260
SatI GCNGC 8 cut(s) 392, 395, 429, 708, 711, 714, 1239, 1242
Sau3AI GATC 8 cut(s) 76, 124, 157, 172, 221, 307, 319, 1213
Sau96I GGNCC 3 cut(s) 399, 601, 1340
ScaI AGTACT 1 cut(s) 991
SchI GAGTC 2 cut(s) 19, 271
ScrFI CCNGG 1 cut(s) 579
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 2 cut(s) 392, 705
SinI GGWCC 3 cut(s) 399, 601, 1340
SmiMI CAYNNNNRTG 1 cut(s) 1083
SpeI ACTAGT 1 cut(s) 986
SsiI CCGC 5 cut(s) 242, 397, 429, 1034, 1239
SspI AATATT 1 cut(s) 875
SspMI CTAG 6 cut(s) 269, 420, 450, 614, 731, 987
StyD4I CCNGG 1 cut(s) 577
StyI CCWWGG 3 cut(s) 120, 687, 1343
TaaI ACNGT 2 cut(s) 563, 571
TaiI ACGT 3 cut(s) 241, 340, 1169
TaqI TCGA 1 cut(s) 954
TaqII GACCGA 1 cut(s) 416
TatI WGTACW 1 cut(s) 989
TauI GCSGC 2 cut(s) 431, 1241
TfiI GAWTC 3 cut(s) 476, 734, 945
Tru1I TTAA 4 cut(s) 839, 1044, 1181, 1260
Tru9I TTAA 4 cut(s) 839, 1044, 1181, 1260
TscAI CASTG 3 cut(s) 285, 384, 979
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 6 cut(s) 391, 394, 707, 710, 713, 1241
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 9 cut(s) 32, 77, 101, 180, 312, 503, 772, 902, 1014
TspGWI ACGGA 1 cut(s) 898
TspRI CASTG 3 cut(s) 285, 384, 979
Tth111I GACNNNGTC 1 cut(s) 1168
VpaK11BI GGWCC 3 cut(s) 399, 601, 1340
VspI ATTAAT 2 cut(s) 1181, 1260
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 5 cut(s) 89, 207, 299, 904, 1220
XceI RCATGY 2 cut(s) 625, 838
XcmI CCANNNNNNNNNTGG 1 cut(s) 755
XspI CTAG 6 cut(s) 269, 420, 450, 614, 731, 987
ZraI GACGTC 1 cut(s) 1167
ZrmI AGTACT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.