Rroxscaffold_3G00235870
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011207
Physical Location & Seq
Forward (+)
23083637 .. 23086372
2736 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00235870.1

Sequence Viewer

Length: 1662 bp
ATGGGGATCAGCAGAGAGAATGATCAGAACGACTTGGAAATTGGTCTTTCACTGAAAATTGATCAGCCTCCTAAAAAAGAACAAGAAGAAGAAGATGACAACGACGACGAAGACGAAGTCGACGAGGATATCGAAGAAGAATCGGCAGCAGTAGCAGCAGCAGAAGAAGAAGAGATCAAAAAGGATAATATTGAAGAAGAACCAAAAGCAGCTGCAGGTGAAGTGGATGATGATGCCTCAGTAGTACTGGAGACAACATATTTGCATGAGAATAAATTGAAGACAGATCAGGAGTTATGGGCTTTGCAAATGGAGATGAGTCGCATGAAAGAAGAAAATAAGGTTTTGAGGAAGGTTGTGGAGCAAACCATGAAGGATTATTACGATCTTCAAATGAAATTCACAGCTGTCCATCAAAATAGCCAAACCAAGGATCCTCGGACCTTTTTATCGCTTCGTGGTAATGATCATGATGAAGCTGATCCTCCAAAAAGTAGTATTCCCAAGATGTCGGATATTTGTCGTCATAGATCTCCATCACCGGCACGTGAGGATGATATCATAAAAGAGAGTCAACTAGGGTTGTCACTGAGGCTACAAACCAATGAATTTAGGGATCAGAGAGAAGATCATGATATCCAAGAACGTAACAAGGAAGAATTGAGGAGTAGTAGTCTCACATCATCAGTGCAACACAAGCTGCAGCAGACCGATTTGGCCGGAATTACTAGCCATGCCGCCCAAGCCAACAGAAAAGCTAGGGTTTCGGTTAGAGCAAGATGTGAATCTGCCACAATGAATGATGGGTGTCAATGGAGGAAATATGGGCAGAAAATCGCGAAAGGAAACCCTTGCCCCAGAGCCTATTACCGTTGCACAGTAGCTCCTGGATGTCCTGTTAGAAAACAGGTCCAAAGATGCCTAGAGGACATGTCCATACTGATAACAACATACGAAGGAACACATAATCATCCACTTCCAGTTGGTGCAACTGCCATGGCTTCCACAGCTTCTGCAGCAGCTGCTTCCTTTATGTTACTAGATTCAAGCAACCACCATCTCTCAGATGGAACACTACCCTCCTCTAATTTCATGACCCAAGCCTCCCTCCCTCCCAATAATTACTACAACACCCCTCACCACATGTTAAACAACCACTACCCCTCATCAATATCTCATCAATATTCCTCCTCATCAAACTTCCGAACCCTAAATTCCGTTCATCATCAGCCTCATCATGACCCTTCAAAAGGAATCGTTCTCGACCTCACCAACAACTTCAGTGATACTTCTACTAGTACTGCCATAGCTTCGGCTGGCTCTTCCTCCCATTCATCTGCACCGCTGGGGTTTAACAATTGGATGGGTAGCAAACCTGCAAATTATCACCATAATGCTGGAAATAGTACGATGATGATGGGCAACCATCATCTGCTGAGTAACAGCAGGAGGACATCTGGGGAAGAAAGTATGACGACGTCGTCAGCATTAATGGCCGAAAATGTGAGTGCTATTGCTTCAGATCCTAAATTTAGGGTTGCTGTTGCAGCTGCTATTACATCTCTCATTAATAGTAAAGAGAATAATACTCATCATCCTACTCCTCCTCATAATAATAATAATTTTGGTCCAAGGGATAATGGAGAGAAAGGTAGTAGCTAA

Protein Analysis

553

Amino Acids

61.37

Weight (kDa)

5.55

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 267 - 325 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
AasI GACNNNNNNGTC 1 cut(s) 1480
AatII GACGTC 1 cut(s) 1481
Acc36I ACCTGC 2 cut(s) 206, 1384
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 839
AciI CCGC 2 cut(s) 738, 1343
AclWI GGATC 6 cut(s) 14, 428, 441, 476, 624, 1517
AcoI YGGCCR 2 cut(s) 717, 1494
AcsI RAATTY 4 cut(s) 398, 608, 1213, 1529
AcuI CTGAAG 2 cut(s) 1264, 1503
AcvI CACGTG 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 1478
AfaI GTAC 3 cut(s) 246, 1300, 1408
AfiI CCNNNNNNNGG 2 cut(s) 430, 1250
AflIII ACRYGT 2 cut(s) 930, 1143
AgsI TTSAA 5 cut(s) 194, 280, 392, 1047, 1248
AhlI ACTAGT 1 cut(s) 1295
AjnI CCWGG 1 cut(s) 886
Alw26I GTCTC 2 cut(s) 245, 680
AlwI GGATC 6 cut(s) 14, 428, 441, 476, 624, 1517
AlwNI CAGNNNCTG 2 cut(s) 1013, 1022
AoxI GGCC 2 cut(s) 717, 1494
ApoI RAATTY 4 cut(s) 398, 608, 1213, 1529
ArsI GACNNNNNNTTYG 4 cut(s) 102, 134, 244, 276
AseI ATTAAT 2 cut(s) 1490, 1569
AspS9I GGNCC 3 cut(s) 441, 910, 1628
AsuHPI GGTGA 5 cut(s) 230, 531, 1130, 1261, 1379
AvaII GGWCC 3 cut(s) 441, 910, 1628
BamHI GGATCC 1 cut(s) 433
BarI GAAGNNNNNNTAC 2 cut(s) 365, 397
BbrPI CACGTG 1 cut(s) 548
BbsI GAAGAC 2 cut(s) 117, 287
BccI CCATC 8 cut(s) 420, 544, 797, 1061, 1065, 1357, 1411, 1434
BciT130I CCWGG 1 cut(s) 888
BclI TGATCA 3 cut(s) 22, 61, 466
BcoDI GTCTC 2 cut(s) 245, 680
BcuI ACTAGT 1 cut(s) 1295
BfaI CTAG 6 cut(s) 578, 729, 759, 923, 1040, 1296
BfmI CTRYAG 3 cut(s) 213, 701, 1014
BfuAI ACCTGC 2 cut(s) 206, 1384
BglII AGATCT 1 cut(s) 530
BmcAI AGTACT 2 cut(s) 246, 1300
Bme1390I CCNGG 1 cut(s) 888
Bme18I GGWCC 3 cut(s) 441, 910, 1628
BmgT120I GGNCC 3 cut(s) 441, 910, 1628
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 888
BmsI GCATC 2 cut(s) 223, 908
BpiI GAAGAC 2 cut(s) 117, 287
BpmI CTGGAG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 2 cut(s) 535, 784
BsaHI GRCGYC 1 cut(s) 1478
BsaJI CCNNGG 4 cut(s) 429, 437, 996, 1631
BsaXI ACNNNNNCTCC 4 cut(s) 658, 688, 868, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 1250
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 252, 980
Bse8I GATNNNNATC 2 cut(s) 535, 784
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 4 cut(s) 429, 437, 996, 1631
BseGI GGATG 6 cut(s) 232, 559, 896, 970, 1368, 1594
BseJI GATNNNNATC 2 cut(s) 535, 784
BseLI CCNNNNNNNGG 2 cut(s) 430, 1250
BseMII CTCAG 4 cut(s) 252, 581, 1077, 1427
BseNI ACTGG 2 cut(s) 252, 980
BseRI GAGGAG 5 cut(s) 679, 1072, 1180, 1593, 1596
BseYI CCCAGC 1 cut(s) 1345
BsgI GTGCAG 1 cut(s) 1323
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 719, 1496
BsiSI CCGG 2 cut(s) 542, 720
BslI CCNNNNNNNGG 2 cut(s) 430, 1250
BsmAI GTCTC 2 cut(s) 245, 680
BsnI GGCC 2 cut(s) 719, 1496
Bsp19I CCATGG 1 cut(s) 996
Bsp68I TCGCGA 1 cut(s) 839
BspACI CCGC 2 cut(s) 738, 1343
BspANI GGCC 2 cut(s) 719, 1496
BspCNI CTCAG 4 cut(s) 251, 582, 1076, 1428
BspFNI CGCG 1 cut(s) 839
BspHI TCATGA 4 cut(s) 469, 631, 1092, 1237
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 3 cut(s) 217, 705, 1018
BspMI ACCTGC 2 cut(s) 206, 1384
BspPI GGATC 6 cut(s) 14, 428, 441, 476, 624, 1517
BspQI GCTCTTC 1 cut(s) 1327
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 2 cut(s) 252, 980
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 4 cut(s) 429, 437, 996, 1631
BssNI GRCGYC 1 cut(s) 1478
BssT1I CCWWGG 3 cut(s) 429, 996, 1631
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 2 cut(s) 872, 880
Bst6I CTCTTC 2 cut(s) 165, 1327
BstACI GRCGYC 1 cut(s) 1478
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 1 cut(s) 1318
BstDEI CTNAG 4 cut(s) 238, 590, 1063, 1436
BstDSI CCRYGG 1 cut(s) 996
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 6 cut(s) 232, 559, 896, 970, 1368, 1594
BstFNI CGCG 1 cut(s) 839
BstMAI GTCTC 2 cut(s) 245, 680
BstMWI GCNNNNNNNGC 9 cut(s) 152, 155, 697, 743, 1007, 1016, 1022, 1493, 1547
BstNI CCWGG 1 cut(s) 888
BstNSI RCATGY 2 cut(s) 934, 1147
BstSCI CCNGG 1 cut(s) 886
BstSFI CTRYAG 3 cut(s) 213, 701, 1014
BstUI CGCG 1 cut(s) 839
BstV2I GAAGAC 2 cut(s) 117, 287
BstX2I RGATCY 3 cut(s) 433, 530, 1522
BstXI CCANNNNNNTGG 1 cut(s) 1397
BstYI RGATCY 3 cut(s) 433, 530, 1522
BsuRI GGCC 2 cut(s) 719, 1496
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 6 cut(s) 232, 559, 896, 970, 1368, 1594
BtsIMutI CAGTG 4 cut(s) 50, 587, 693, 1288
BtuMI TCGCGA 1 cut(s) 839
BveI ACCTGC 2 cut(s) 206, 1384
Cac8I GCNNGC 1 cut(s) 1318
CaiI CAGNNNCTG 2 cut(s) 1013, 1022
CciI TCATGA 4 cut(s) 469, 631, 1092, 1237
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 3 cut(s) 441, 910, 1628
Csp6I GTAC 3 cut(s) 245, 1299, 1407
CviQI GTAC 3 cut(s) 245, 1299, 1407
DdeI CTNAG 4 cut(s) 238, 590, 1063, 1436
DrdI GACNNNNNNGTC 1 cut(s) 1480
DseDI GACNNNNNNGTC 1 cut(s) 1480
EaeI YGGCCR 2 cut(s) 717, 1494
Eam1104I CTCTTC 2 cut(s) 165, 1327
EarI CTCTTC 2 cut(s) 165, 1327
Eco130I CCWWGG 3 cut(s) 429, 996, 1631
Eco32I GATATC 3 cut(s) 130, 559, 637
Eco47I GGWCC 3 cut(s) 441, 910, 1628
Eco57I CTGAAG 2 cut(s) 1264, 1503
Eco72I CACGTG 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRII CCWGG 1 cut(s) 886
EcoRV GATATC 3 cut(s) 130, 559, 637
EcoT14I CCWWGG 3 cut(s) 429, 996, 1631
ErhI CCWWGG 3 cut(s) 429, 996, 1631
FbaI TGATCA 3 cut(s) 22, 61, 466
FblI GTMKAC 1 cut(s) 120
FokI GGATG 6 cut(s) 239, 566, 903, 957, 1375, 1581
FspBI CTAG 6 cut(s) 578, 729, 759, 923, 1040, 1296
GsaI CCCAGC 1 cut(s) 1349
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 719, 1496
HapII CCGG 2 cut(s) 542, 720
Hin1I GRCGYC 1 cut(s) 1478
HincII GTYRAC 2 cut(s) 121, 575
HindII GTYRAC 2 cut(s) 121, 575
HinfI GANTC 6 cut(s) 140, 319, 571, 785, 1043, 1254
HpaII CCGG 2 cut(s) 542, 720
HphI GGTGA 5 cut(s) 230, 531, 1130, 1261, 1379
Hpy166II GTNNAC 2 cut(s) 121, 575
Hpy188I TCNGA 7 cut(s) 27, 441, 514, 621, 1066, 1205, 1522
Hpy188III TCNNGA 7 cut(s) 290, 470, 632, 838, 1093, 1238, 1262
Hpy8I GTNNAC 2 cut(s) 121, 575
Hpy99I CGWCG 5 cut(s) 107, 110, 125, 1480, 1483
HpyAV CCTTC 4 cut(s) 346, 367, 950, 1254
HpyCH4III ACNGT 2 cut(s) 872, 880
HpyCH4IV ACGT 3 cut(s) 547, 646, 1478
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 155, 697, 743, 1007, 1016, 1022, 1493, 1547
HpyF3I CTNAG 4 cut(s) 238, 590, 1063, 1436
HpySE526I ACGT 3 cut(s) 547, 646, 1478
Hsp92I GRCGYC 1 cut(s) 1478
Ksp22I TGATCA 3 cut(s) 22, 61, 466
LguI GCTCTTC 1 cut(s) 1327
LmnI GCTCC 2 cut(s) 361, 889
LweI GCATC 2 cut(s) 223, 908
MaeI CTAG 6 cut(s) 578, 729, 759, 923, 1040, 1296
MaeII ACGT 3 cut(s) 547, 646, 1478
MaeIII GTNAC 4 cut(s) 585, 647, 1035, 1439
MfeI CAATTG 1 cut(s) 1357
MflI RGATCY 3 cut(s) 433, 530, 1522
MlyI GAGTC 2 cut(s) 328, 580
MmeI TCCRAC 1 cut(s) 492
MseI TTAA 4 cut(s) 1148, 1353, 1490, 1569
MslI CAYNNNNRTG 1 cut(s) 1392
MspA1I CMGCKG 5 cut(s) 212, 407, 1022, 1345, 1550
MspI CCGG 2 cut(s) 542, 720
MspR9I CCNGG 1 cut(s) 888
MunI CAATTG 1 cut(s) 1357
MvaI CCWGG 1 cut(s) 888
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 9 cut(s) 152, 155, 697, 743, 1007, 1016, 1022, 1493, 1547
NcoI CCATGG 1 cut(s) 996
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 585
NruI TCGCGA 1 cut(s) 839
NspI RCATGY 2 cut(s) 934, 1147
PagI TCATGA 4 cut(s) 469, 631, 1092, 1237
PaqCI CACCTGC 1 cut(s) 206
PciI ACATGT 2 cut(s) 930, 1143
PciSI GCTCTTC 1 cut(s) 1327
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 120, 129
PfeI GAWTC 4 cut(s) 140, 785, 1043, 1254
PflFI GACNNNGTC 3 cut(s) 116, 1477, 1480
PfoI TCCNGGA 1 cut(s) 886
PleI GAGTC 2 cut(s) 327, 579
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 2 cut(s) 327, 579
Ppu21I YACGTR 1 cut(s) 548
PscI ACATGT 2 cut(s) 930, 1143
PshBI ATTAAT 2 cut(s) 1490, 1569
Psp6I CCWGG 1 cut(s) 886
PspCI CACGTG 1 cut(s) 548
PspFI CCCAGC 1 cut(s) 1345
PspGI CCWGG 1 cut(s) 886
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 3 cut(s) 441, 910, 1628
PstI CTGCAG 3 cut(s) 217, 705, 1018
PstNI CAGNNNCTG 2 cut(s) 1013, 1022
PsuI RGATCY 3 cut(s) 433, 530, 1522
PsyI GACNNNGTC 3 cut(s) 116, 1477, 1480
PvuII CAGCTG 4 cut(s) 212, 407, 1022, 1550
RruI TCGCGA 1 cut(s) 839
RsaI GTAC 3 cut(s) 246, 1300, 1408
RsaNI GTAC 3 cut(s) 245, 1299, 1407
RseI CAYNNNNRTG 1 cut(s) 1392
SalI GTCGAC 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 1327
SaqAI TTAA 4 cut(s) 1148, 1353, 1490, 1569
Sau96I GGNCC 3 cut(s) 441, 910, 1628
ScaI AGTACT 2 cut(s) 246, 1300
SchI GAGTC 2 cut(s) 328, 580
ScrFI CCNGG 1 cut(s) 888
SfaNI GCATC 2 cut(s) 223, 908
SfcI CTRYAG 3 cut(s) 213, 701, 1014
SinI GGWCC 3 cut(s) 441, 910, 1628
SmiMI CAYNNNNRTG 1 cut(s) 1392
SpeI ACTAGT 1 cut(s) 1295
SsiI CCGC 2 cut(s) 738, 1343
SspI AATATT 2 cut(s) 190, 1184
SspMI CTAG 6 cut(s) 578, 729, 759, 923, 1040, 1296
StyD4I CCNGG 1 cut(s) 886
StyI CCWWGG 3 cut(s) 429, 996, 1631
TaaI ACNGT 2 cut(s) 872, 880
TaiI ACGT 3 cut(s) 550, 649, 1481
TaqI TCGA 3 cut(s) 120, 132, 1263
TaqII GACCGA 1 cut(s) 725
TatI WGTACW 2 cut(s) 244, 1298
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 4 cut(s) 140, 785, 1043, 1254
Tru1I TTAA 4 cut(s) 1148, 1353, 1490, 1569
Tru9I TTAA 4 cut(s) 1148, 1353, 1490, 1569
TscAI CASTG 4 cut(s) 57, 594, 693, 1288
TseFI GTSAC 1 cut(s) 585
Tsp45I GTSAC 1 cut(s) 585
TspDTI ATGAA 9 cut(s) 341, 386, 410, 489, 621, 812, 1081, 1211, 1323
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 4 cut(s) 57, 594, 693, 1288
Tth111I GACNNNGTC 3 cut(s) 116, 1477, 1480
VpaK11BI GGWCC 3 cut(s) 441, 910, 1628
VspI ATTAAT 2 cut(s) 1490, 1569
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 4 cut(s) 398, 608, 1213, 1529
XceI RCATGY 2 cut(s) 934, 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 1064
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 6 cut(s) 578, 729, 759, 923, 1040, 1296
ZraI GACGTC 1 cut(s) 1479
ZrmI AGTACT 2 cut(s) 246, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.