MD08G1227200.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011207
Physical Location & Seq
Forward (+)
29356105 .. 29358646
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1227200.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGGGGAATGGAGACAAGGAGAAAGACTTGGATGTTGATCTCTCACTCAAAATTGATGCAAAAGAAGAAAAACAAGAAGAAGGTGTAGGCGGCGAGGAAGAAAAAGAAGAAGAAGATGCTCATCGCGTCAACGATGGCGTTGAATCAACAGCAGCAGAAGAAAAGGAGGATGAGCAACAACCAGGAGTACAGAATAAGAGGCGAAAAACAACGGAAACCGGGGCCGGGGAAGTGGAAGACGATGATGTCTCAGTAGTCGAAACATCGGTGCAGGAGAATAATATGAAGACGGATCAAGAGTTAAGCGTTTTACAAATGGAGATGAGCCGTATGAAAGAAGAAAACGAGGTTTTGAGGAAGGTCGTAGAGCAAACCATGAAAGATTATAATGATCTTCAAATGAAATTTACGGTTCTCCATCAAAGTTGCCAAAACAAGGATCCTCAAACCTTTCTATCACTTGCTGGTAACGATCCCAACGCAGTTCAAGGACCCAGGAAGATTACCAAGATGTCAGATATTCGCCATAGATCTCCACTGTTACCAAAACGTGATGATGAGAGTCAACTAGGGTTGTCACTGAGGCTAACCAGCTCTGCTACTACTGCCAGTCAATTAAGGGGAAGAGAAGAAGGAGAAGAAGACGATGATGACAAAGAAGAGATCAATAACAAGGAAGGATTGACGAGGACTACTAGTCTTACATCATCGTCAGTGCAACTGCAAAACAAGTTACAGCGGCCGGAATTGGCCGGAACTCCAAGCCACGGTGGCTCCCAACCCAATCGAAAAGCTAGGGTTTCGGTTAGATCAAGATGTGAATCGGCCGCGATAAATGATGGGTGCAGTTGGAGGAAATATGGGCAGAAAATCTCAAAAGGAAACCCTTGCCCACGAGCCTATTATCGTTGCACAGTAGTCGCGGGATGCCCAGTCAGAAAACAGGTCCAAAGATGCCAAGAGGACATGTCCATACTTATAACAACTTATGAAGGAACACACAACCATCCTCTTCCAGTTGGTGCGACGGCCATGGGTGCAACAGCCATGGCTTCAACAGCTGCTGCTGCAGCTTCCTTTATGTTACTAGATTCAAGTAACCTTCATTTAGCAGATGGCACACCCTCTACTTACACCCAAGCATCCCTCCCTTACCATCATATGATGAACATGAACCCTTCATCTCATTCCTCGTCAAACTTGCGAAGCGTCGATCCTCATGACCCTTCCAAAGGACTTGTTCTTGACCTCACGAACAACTTCTATGACACCATGGGTGGCAGCTCCTCATCTATACCACCAGGTTTTAGTACTAATTGGTTGATGAGCAGCACTAGCAGCAGATCTGCAAATTACCCTAGCAGTACTAGTCTCACCAACCGTCTTTTGGATAACGGCAGTAGATCAACCAGAGTGGCGCTAGGGGACGATCAGAAAGGTAATTGGAGATCAGGGGAAGAAAATATGTCCATGGCTGAAAATGTAAGTGCCATTGCTTCAGATCCCAAATTTAGAGTTGCTGTTGCAGCTGCTATCACATCCCTTATTAACAAAGATAACCATACAACAACTAGTCATCCTGCGCCGAATGCTATTGCCCCAAGAGATCATGGAGACAACAACGGTAGTTCATCTACTAGCAACCTTTGGGCTATTGATGAACATTTTCTCTCATCCAACGGTAAGCTCATTCGCAAAAACACCTCAGAATAA

Protein Analysis

571

Amino Acids

62.31

Weight (kDa)

5.36

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 279 - 337 8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68150
fragaria_vesca FvH4_5g29820 FvH4_5g29820 FvH4_5g29820
malus_domestica MD08G1227200.v1.1 MD15G1419600.v1.1
prunus_persica Prupe.1G564300_v2.0.a1
pyrus_communis pycom08g19820
rosa_chinensis RchiOBHm_Chr7g0223361
rosa_laevigata RLG00000001986
rosa_multiflora Rmu_sc0005124.1_g000024 Rmu_sc0009107.1_g000007
rosa_roxburghii Rroxscaffold_3G00235870
rosa_rugosa Rorug07G0216200 Rorug07G0216300
rosa_samantha Rh7AG360000 Rh7BG349800 Rh7CG378400 Rh7DG355200
rosa_wichuraiana Rw7G030590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 387, 980
AasI GACNNNNNNGTC 1 cut(s) 245
AccII CGCG 3 cut(s) 126, 830, 923
AciI CCGC 4 cut(s) 90, 739, 828, 923
AclWI GGATC 6 cut(s) 300, 434, 447, 467, 1208, 1496
AcoI YGGCCR 4 cut(s) 740, 750, 825, 1029
AcsI RAATTY 2 cut(s) 404, 1508
AcuI CTGAAG 1 cut(s) 1482
AfaI GTAC 3 cut(s) 189, 1312, 1366
AfiI CCNNNNNNNGG 5 cut(s) 225, 436, 767, 1232, 1387
AflIII ACRYGT 1 cut(s) 966
AgsI TTSAA 5 cut(s) 143, 398, 488, 1056, 1095
AhdI GACNNNNNGTC 1 cut(s) 696
AhlI ACTAGT 3 cut(s) 695, 1367, 1571
AjnI CCWGG 3 cut(s) 181, 494, 1300
AluBI AGCT 7 cut(s) 594, 794, 1061, 1073, 1284, 1529, 1687
AluI AGCT 7 cut(s) 594, 794, 1061, 1073, 1284, 1529, 1687
Alw26I GTCTC 4 cut(s) 6, 253, 1376, 1608
AlwI GGATC 6 cut(s) 300, 434, 447, 467, 1208, 1496
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 5 cut(s) 222, 740, 750, 825, 1029
ApoI RAATTY 2 cut(s) 404, 1508
Asp700I GAANNNNTTC 2 cut(s) 1259, 1665
AspLEI GCGC 2 cut(s) 1420, 1585
AspS9I GGNCC 3 cut(s) 222, 491, 946
AsuC2I CCSGG 2 cut(s) 220, 226
AsuHPI GGTGA 1 cut(s) 1366
AvaII GGWCC 2 cut(s) 491, 946
BamHI GGATCC 1 cut(s) 439
BauI CACGAG 1 cut(s) 894
BbsI GAAGAC 3 cut(s) 243, 293, 648
BccI CCATC 6 cut(s) 128, 426, 833, 1014, 1109, 1164
BceAI ACGGC 3 cut(s) 312, 1044, 1411
BcgI CGANNNNNNTGC 2 cut(s) 901, 935
BciT130I CCWGG 3 cut(s) 183, 496, 1302
BcnI CCSGG 2 cut(s) 220, 226
BcoDI GTCTC 4 cut(s) 6, 253, 1376, 1608
BcuI ACTAGT 3 cut(s) 695, 1367, 1571
BfmI CTRYAG 1 cut(s) 1068
BfoI RGCGCY 1 cut(s) 1421
BglI GCCNNNNNGGC 1 cut(s) 771
BglII AGATCT 2 cut(s) 530, 1343
BmcAI AGTACT 2 cut(s) 1312, 1366
Bme1390I CCNGG 5 cut(s) 183, 220, 226, 496, 1302
Bme18I GGWCC 2 cut(s) 491, 946
BmeRI GACNNNNNGTC 1 cut(s) 696
BmgT120I GGNCC 3 cut(s) 222, 491, 946
BmiI GGNNCC 4 cut(s) 223, 441, 493, 775
BmrFI CCNGG 5 cut(s) 183, 220, 226, 496, 1302
BmrI ACTGGG 1 cut(s) 926
BmsI GCATC 5 cut(s) 46, 106, 917, 944, 1151
BmuI ACTGGG 1 cut(s) 926
BpiI GAAGAC 3 cut(s) 243, 293, 648
BpuMI CCSGG 2 cut(s) 220, 226
BsaBI GATNNNNATC 3 cut(s) 36, 120, 820
BsaJI CCNNGG 8 cut(s) 219, 225, 494, 766, 1032, 1047, 1272, 1470
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 436, 767, 1232, 1387
Bse1I ACTGG 3 cut(s) 609, 932, 1016
Bse3DI GCAATG 1 cut(s) 1491
Bse8I GATNNNNATC 3 cut(s) 36, 120, 820
BseBI CCWGG 3 cut(s) 183, 496, 1302
BseDI CCNNGG 8 cut(s) 219, 225, 494, 766, 1032, 1047, 1272, 1470
BseGI GGATG 8 cut(s) 37, 175, 932, 1006, 1142, 1538, 1576, 1673
BseJI GATNNNNATC 3 cut(s) 36, 120, 820
BseLI CCNNNNNNNGG 5 cut(s) 225, 436, 767, 1232, 1387
BseMI GCAATG 1 cut(s) 1491
BseMII CTCAG 2 cut(s) 264, 572
BseNI ACTGG 3 cut(s) 609, 932, 1016
BseRI GAGGAG 1 cut(s) 1276
BseX3I CGGCCG 2 cut(s) 740, 825
BsgI GTGCAG 2 cut(s) 290, 865
Bsh1236I CGCG 3 cut(s) 126, 830, 923
Bsh1285I CGRYCG 2 cut(s) 743, 828
BshFI GGCC 5 cut(s) 224, 742, 752, 827, 1031
BsiEI CGRYCG 2 cut(s) 743, 828
BsiSI CCGG 4 cut(s) 219, 225, 743, 753
BslFI GGGAC 1 cut(s) 1439
BslI CCNNNNNNNGG 5 cut(s) 225, 436, 767, 1232, 1387
BsmAI GTCTC 4 cut(s) 6, 253, 1376, 1608
BsmFI GGGAC 1 cut(s) 1439
BsmI GAATGC 1 cut(s) 1594
BsnI GGCC 5 cut(s) 224, 742, 752, 827, 1031
Bsp19I CCATGG 4 cut(s) 1032, 1047, 1272, 1470
BspACI CCGC 4 cut(s) 90, 739, 828, 923
BspANI GGCC 5 cut(s) 224, 742, 752, 827, 1031
BspCNI CTCAG 2 cut(s) 263, 573
BspFNI CGCG 3 cut(s) 126, 830, 923
BspHI TCATGA 1 cut(s) 1219
BspLI GGNNCC 4 cut(s) 223, 441, 493, 775
BspMAI CTGCAG 1 cut(s) 1072
BspPI GGATC 6 cut(s) 300, 434, 447, 467, 1208, 1496
BsrDI GCAATG 1 cut(s) 1491
BsrI ACTGG 3 cut(s) 609, 932, 1016
BssECI CCNNGG 8 cut(s) 219, 225, 494, 766, 1032, 1047, 1272, 1470
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 4 cut(s) 1032, 1047, 1272, 1470
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 3 cut(s) 183, 496, 1302
Bst4CI ACNGT 7 cut(s) 412, 540, 770, 916, 1382, 1625, 1682
Bst6I CTCTTC 3 cut(s) 619, 654, 1017
BstDEI CTNAG 3 cut(s) 250, 581, 1705
BstDSI CCRYGG 5 cut(s) 766, 1032, 1047, 1272, 1470
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 8 cut(s) 37, 175, 932, 1006, 1142, 1538, 1576, 1673
BstFNI CGCG 3 cut(s) 126, 830, 923
BstH2I RGCGCY 1 cut(s) 1421
BstHHI GCGC 2 cut(s) 1420, 1585
BstMAI GTCTC 4 cut(s) 6, 253, 1376, 1608
BstMCI CGRYCG 2 cut(s) 743, 828
BstNI CCWGG 3 cut(s) 183, 496, 1302
BstNSI RCATGY 1 cut(s) 970
BstSCI CCNGG 5 cut(s) 181, 218, 224, 494, 1300
BstSFI CTRYAG 1 cut(s) 1068
BstUI CGCG 3 cut(s) 126, 830, 923
BstV2I GAAGAC 3 cut(s) 243, 293, 648
BstX2I RGATCY 4 cut(s) 439, 530, 1343, 1501
BstYI RGATCY 4 cut(s) 439, 530, 1343, 1501
BstZI CGGCCG 2 cut(s) 740, 825
BsuRI GGCC 5 cut(s) 224, 742, 752, 827, 1031
BtgI CCRYGG 5 cut(s) 766, 1032, 1047, 1272, 1470
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 8 cut(s) 37, 175, 932, 1006, 1142, 1538, 1576, 1673
BtsIMutI CAGTG 3 cut(s) 536, 578, 720
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 1 cut(s) 1219
CfoI GCGC 2 cut(s) 1420, 1585
Cfr13I GGNCC 3 cut(s) 222, 491, 946
CseI GACGC 2 cut(s) 115, 1198
CsiI ACCWGGT 1 cut(s) 1300
Csp6I GTAC 3 cut(s) 188, 1311, 1365
CspCI CAANNNNNGTGG 2 cut(s) 1395, 1430
CviAII CATG 9 cut(s) 376, 967, 1033, 1048, 1171, 1220, 1273, 1471, 1610
CviQI GTAC 3 cut(s) 188, 1311, 1365
DdeI CTNAG 3 cut(s) 250, 581, 1705
DrdI GACNNNNNNGTC 1 cut(s) 245
DriI GACNNNNNGTC 1 cut(s) 696
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 4 cut(s) 740, 750, 825, 1029
EagI CGGCCG 2 cut(s) 740, 825
Eam1104I CTCTTC 3 cut(s) 619, 654, 1017
Eam1105I GACNNNNNGTC 1 cut(s) 696
EarI CTCTTC 3 cut(s) 619, 654, 1017
EclXI CGGCCG 2 cut(s) 740, 825
Eco130I CCWWGG 4 cut(s) 1032, 1047, 1272, 1470
Eco47I GGWCC 2 cut(s) 491, 946
Eco52I CGGCCG 2 cut(s) 740, 825
Eco57I CTGAAG 1 cut(s) 1482
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 181, 494, 1300
EcoT14I CCWWGG 4 cut(s) 1032, 1047, 1272, 1470
ErhI CCWWGG 4 cut(s) 1032, 1047, 1272, 1470
FaeI CATG 9 cut(s) 379, 970, 1036, 1051, 1174, 1223, 1276, 1474, 1613
FaqI GGGAC 1 cut(s) 1439
FatI CATG 9 cut(s) 375, 966, 1032, 1047, 1170, 1219, 1272, 1470, 1609
FauI CCCGC 1 cut(s) 916
FauNDI CATATG 1 cut(s) 1161
FokI GGATG 8 cut(s) 44, 182, 939, 993, 1129, 1525, 1563, 1660
GlaI GCGC 2 cut(s) 1419, 1584
HaeII RGCGCY 1 cut(s) 1421
HaeIII GGCC 5 cut(s) 224, 742, 752, 827, 1031
HapII CCGG 4 cut(s) 219, 225, 743, 753
HgaI GACGC 2 cut(s) 115, 1198
HhaI GCGC 2 cut(s) 1420, 1585
Hin1II CATG 9 cut(s) 379, 970, 1036, 1051, 1174, 1223, 1276, 1474, 1613
Hin6I GCGC 2 cut(s) 1418, 1583
HinP1I GCGC 2 cut(s) 1418, 1583
HincII GTYRAC 2 cut(s) 130, 566
HindII GTYRAC 2 cut(s) 130, 566
HinfI GANTC 4 cut(s) 143, 562, 821, 1091
HpaII CCGG 4 cut(s) 219, 225, 743, 753
HphI GGTGA 1 cut(s) 1366
Hpy166II GTNNAC 2 cut(s) 130, 566
Hpy188I TCNGA 5 cut(s) 517, 938, 1434, 1501, 1708
Hpy188III TCNNGA 5 cut(s) 296, 813, 1220, 1244, 1252
Hpy8I GTNNAC 2 cut(s) 130, 566
Hpy99I CGWCG 2 cut(s) 1030, 1214
HpyAV CCTTC 8 cut(s) 74, 352, 626, 671, 986, 1112, 1188, 1236
HpyCH4III ACNGT 7 cut(s) 412, 540, 770, 916, 1382, 1625, 1682
HpyCH4IV ACGT 1 cut(s) 550
HpyF3I CTNAG 3 cut(s) 250, 581, 1705
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 9 cut(s) 379, 970, 1036, 1051, 1174, 1223, 1276, 1474, 1613
HspAI GCGC 2 cut(s) 1418, 1583
LmnI GCTCC 2 cut(s) 779, 1289
LweI GCATC 5 cut(s) 46, 106, 917, 944, 1151
MabI ACCWGGT 1 cut(s) 1300
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 6 cut(s) 467, 540, 576, 732, 1083, 1097
MflI RGATCY 4 cut(s) 439, 530, 1343, 1501
MluCI AATT 8 cut(s) 51, 404, 614, 746, 1315, 1351, 1441, 1508
MlyI GAGTC 1 cut(s) 571
MmeI TCCRAC 2 cut(s) 830, 1701
MroXI GAANNNNTTC 2 cut(s) 1259, 1665
MseI TTAA 3 cut(s) 302, 617, 1548
MspA1I CMGCKG 3 cut(s) 739, 1061, 1529
MspI CCGG 4 cut(s) 219, 225, 743, 753
MspR9I CCNGG 5 cut(s) 183, 220, 226, 496, 1302
Mva1269I GAATGC 1 cut(s) 1594
MvaI CCWGG 3 cut(s) 183, 496, 1302
MvnI CGCG 3 cut(s) 126, 830, 923
NciI CCSGG 2 cut(s) 220, 226
NcoI CCATGG 4 cut(s) 1032, 1047, 1272, 1470
NdeI CATATG 1 cut(s) 1161
NlaIII CATG 9 cut(s) 379, 970, 1036, 1051, 1174, 1223, 1276, 1474, 1613
NlaIV GGNNCC 4 cut(s) 223, 441, 493, 775
NmuCI GTSAC 1 cut(s) 576
NspI RCATGY 1 cut(s) 970
PagI TCATGA 1 cut(s) 1219
PciI ACATGT 1 cut(s) 966
PctI GAATGC 1 cut(s) 1594
PdmI GAANNNNTTC 2 cut(s) 1259, 1665
PfeI GAWTC 3 cut(s) 143, 821, 1091
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
PpuMI RGGWCCY 1 cut(s) 491
PscI ACATGT 1 cut(s) 966
PsiI TTATAA 2 cut(s) 387, 980
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 3 cut(s) 181, 494, 1300
PspGI CCWGG 3 cut(s) 181, 494, 1300
PspN4I GGNNCC 4 cut(s) 223, 441, 493, 775
PspPI GGNCC 3 cut(s) 222, 491, 946
PspPPI RGGWCCY 1 cut(s) 491
PstI CTGCAG 1 cut(s) 1072
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 4 cut(s) 439, 530, 1343, 1501
PvuII CAGCTG 2 cut(s) 1061, 1529
RsaI GTAC 3 cut(s) 189, 1312, 1366
RsaNI GTAC 3 cut(s) 188, 1311, 1365
SaqAI TTAA 3 cut(s) 302, 617, 1548
Sau96I GGNCC 3 cut(s) 222, 491, 946
ScaI AGTACT 2 cut(s) 1312, 1366
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 5 cut(s) 183, 220, 226, 496, 1302
SexAI ACCWGGT 1 cut(s) 1300
SfaNI GCATC 5 cut(s) 46, 106, 917, 944, 1151
SfcI CTRYAG 1 cut(s) 1068
SinI GGWCC 2 cut(s) 491, 946
SpeI ACTAGT 3 cut(s) 695, 1367, 1571
Sse9I AATT 8 cut(s) 51, 404, 614, 746, 1315, 1351, 1441, 1508
SsiI CCGC 4 cut(s) 90, 739, 828, 923
StyD4I CCNGG 5 cut(s) 181, 218, 224, 494, 1300
StyI CCWWGG 4 cut(s) 1032, 1047, 1272, 1470
TaaI ACNGT 7 cut(s) 412, 540, 770, 916, 1382, 1625, 1682
TaiI ACGT 1 cut(s) 553
TaqI TCGA 3 cut(s) 258, 787, 1212
TasI AATT 8 cut(s) 51, 404, 614, 746, 1315, 1351, 1441, 1508
TatI WGTACW 3 cut(s) 187, 1310, 1364
TauI GCSGC 3 cut(s) 93, 742, 830
TfiI GAWTC 3 cut(s) 143, 821, 1091
Tru1I TTAA 3 cut(s) 302, 617, 1548
Tru9I TTAA 3 cut(s) 302, 617, 1548
TscAI CASTG 3 cut(s) 543, 585, 720
TseFI GTSAC 1 cut(s) 576
Tsp45I GTSAC 1 cut(s) 576
TspGWI ACGGA 2 cut(s) 227, 305
TspRI CASTG 3 cut(s) 543, 585, 720
VpaK11BI GGWCC 2 cut(s) 491, 946
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 2 cut(s) 404, 1508
XceI RCATGY 1 cut(s) 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 1384
XmnI GAANNNNTTC 2 cut(s) 1259, 1665
ZrmI AGTACT 2 cut(s) 1312, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.