AT1G68180

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013457
Physical Location & Seq
Forward (+)
25554721 .. 25556097
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68180.1

Sequence Viewer

Length: 708 bp
ATGACATCTGCTTATCGTCCAAGAGTTATTGTGAATGGAACAAGAAGAACAAGAACGTTCCATTACTTCTATTGTCGCCATTGTAGCCGTACCATTAGGCTAAGAAGCTTTGGTCTATATGGTCCCATATGTCCTTTCTGTTCTAGAGAGATCAATCTTCACGATGAGCTTGACATTATGAGGCCAAGCCGACCTTATTGGGATAGTGATACCGATTGGATCACTCTCCATCTTATCAACGAACCAAGAAGTAACCGGTTCAATCATGATGATCTAGTTTATAATACTGACGAGGAATTCGCTGATGTCATGCCTAGTGTACAAATCGGTCCACCACCAGCCTCGCAATCCGCCATCGAGGCTGTAAGGACGGTGATAATCACGGATGAAGATTTGGTGAAAGAGAAGGTTTGTGCGATATGCAAGGAGGAATTTGAAGTTGGAGAGGAAGGAAAAGAATTGAAATGCTTGCATTTGTACCATTCAAGCTGCATTGTGTCTTGGTTGAACATTCACAACACTTGTCCCATTTGTCGTTTTGAGGTTAACTTAGGTGTTTCCGAGAGTAACGTAGACGAAGGAGGGTCTTACAACATCGACAATGATCGATCAAATCGGTTTCGGACTCGGGTTTGTTCTTTGTGGCCTCTCCGAATGATGATTGATTGGGTACATAATCTCATAGATATAAGAATAACAAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

235

Amino Acids

27.42

Weight (kDa)

5.85

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 179 6.1e-12 Ring finger domain
zf-C3HC4 PF00097 138 - 178 7.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 138 - 178 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 138 - 165 3.1e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68180
fragaria_vesca FvH4_5g29600 FvH4_5g29600
malus_domestica MD08G1228300.v1.1 MD15G1422200.v1.1
prunus_persica Prupe.1G567500_v2.0.a1 Prupe.1G567500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0222631
rosa_laevigata RLG00000002042
rosa_multiflora Rmu_sc0001498.1_g000010
rosa_roxburghii Rroxscaffold_3G00236580
rosa_rugosa Rorug07G0211600
rosa_samantha Rh7AG355200 Rh7BG343500 Rh7CG372500 Rh7DG349700
rosa_wichuraiana Rw7G029810 Rw7G030160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AccI GTMKAC 1 cut(s) 573
AciI CCGC 1 cut(s) 351
AclI AACGTT 1 cut(s) 56
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 2 cut(s) 296, 431
AfaI GTAC 4 cut(s) 91, 321, 479, 672
AgeI ACCGGT 1 cut(s) 255
AgsI TTSAA 5 cut(s) 262, 437, 463, 486, 508
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 3 cut(s) 108, 169, 489
AluI AGCT 3 cut(s) 108, 169, 489
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 627
AoxI GGCC 2 cut(s) 182, 644
ApeKI GCWGC 1 cut(s) 489
ApoI RAATTY 2 cut(s) 296, 431
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AsiGI ACCGGT 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 122, 329
AsuHPI GGTGA 2 cut(s) 385, 409
AvaI CYCGRG 1 cut(s) 627
AvaII GGWCC 2 cut(s) 122, 329
BbvI GCAGC 1 cut(s) 476
BccI CCATC 2 cut(s) 237, 362
BceAI ACGGC 1 cut(s) 72
BfaI CTAG 3 cut(s) 144, 275, 315
BglI GCCNNNNNGGC 1 cut(s) 359
BisI GCNGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 122, 329
BmeT110I CYCGRG 1 cut(s) 627
BmgT120I GGNCC 2 cut(s) 122, 329
BmiI GGNNCC 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 607
BsaWI WCCGGW 1 cut(s) 255
Bse118I RCCGGY 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 607
BseGI GGATG 1 cut(s) 391
BseXI GCAGC 1 cut(s) 476
BshFI GGCC 2 cut(s) 184, 646
BshTI ACCGGT 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 607
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 1 cut(s) 256
BslFI GGGAC 2 cut(s) 108, 510
BsmFI GGGAC 2 cut(s) 108, 510
BsnI GGCC 2 cut(s) 184, 646
BsoBI CYCGRG 1 cut(s) 627
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 5 cut(s) 150, 219, 271, 604, 608
BspACI CCGC 1 cut(s) 351
BspANI GGCC 2 cut(s) 184, 646
BspDI ATCGAT 1 cut(s) 607
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 124
BspPI GGATC 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 255
BsrGI TGTACA 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 255
BssMI GATC 5 cut(s) 150, 219, 271, 604, 608
Bst4CI ACNGT 1 cut(s) 373
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 101, 550
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 5 cut(s) 153, 222, 274, 607, 611
BstMBI GATC 5 cut(s) 150, 219, 271, 604, 608
BstMWI GCNNNNNNNGC 2 cut(s) 84, 359
BstV1I GCAGC 1 cut(s) 476
Bsu15I ATCGAT 1 cut(s) 607
BsuRI GGCC 2 cut(s) 184, 646
BsuTUI ATCGAT 1 cut(s) 607
BtsCI GGATG 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 470
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 122, 329
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 4 cut(s) 90, 320, 478, 671
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 2 cut(s) 266, 310
CviQI GTAC 4 cut(s) 90, 320, 478, 671
DdeI CTNAG 2 cut(s) 101, 550
DpnI GATC 5 cut(s) 152, 221, 273, 606, 610
DpnII GATC 5 cut(s) 150, 219, 271, 604, 608
EciI GGCGGA 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 122, 329
Eco88I CYCGRG 1 cut(s) 627
EcoRI GAATTC 1 cut(s) 296
FaeI CATG 2 cut(s) 269, 313
FalI AAGNNNNNCTT 2 cut(s) 178, 210
FaqI GGGAC 2 cut(s) 108, 510
FatI CATG 2 cut(s) 265, 309
FauNDI CATATG 1 cut(s) 128
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 490
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 1 cut(s) 490
FspBI CTAG 3 cut(s) 144, 275, 315
GluI GCNGC 1 cut(s) 490
HaeIII GGCC 2 cut(s) 184, 646
HapII CCGG 1 cut(s) 256
Hin1II CATG 2 cut(s) 269, 313
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 1 cut(s) 625
HpaI GTTAAC 1 cut(s) 547
HpaII CCGG 1 cut(s) 256
HphI GGTGA 2 cut(s) 385, 409
Hpy166II GTNNAC 4 cut(s) 320, 332, 547, 574
Hpy188I TCNGA 3 cut(s) 562, 624, 653
Hpy188III TCNNGA 4 cut(s) 144, 161, 266, 705
Hpy8I GTNNAC 4 cut(s) 320, 332, 547, 574
HpyAV CCTTC 3 cut(s) 400, 443, 572
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 2 cut(s) 56, 570
HpyCH4V TGCA 3 cut(s) 423, 472, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 359
HpyF3I CTNAG 2 cut(s) 101, 550
HpySE526I ACGT 2 cut(s) 56, 570
Hsp92II CATG 2 cut(s) 269, 313
KspAI GTTAAC 1 cut(s) 547
Kzo9I GATC 5 cut(s) 150, 219, 271, 604, 608
LpnPI CCDG 2 cut(s) 269, 351
Lsp1109I GCAGC 1 cut(s) 476
MaeI CTAG 3 cut(s) 144, 275, 315
MaeII ACGT 2 cut(s) 56, 570
MaeIII GTNAC 2 cut(s) 251, 566
MalI GATC 5 cut(s) 152, 221, 273, 606, 610
MboI GATC 5 cut(s) 150, 219, 271, 604, 608
MboII GAAGA 3 cut(s) 57, 149, 401
MluCI AATT 3 cut(s) 296, 431, 458
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 9 cut(s) 174, 286, 352, 352, 421, 439, 535, 575, 657
MseI TTAA 1 cut(s) 546
MspI CCGG 1 cut(s) 256
MwoI GCNNNNNNNGC 2 cut(s) 84, 359
NdeI CATATG 1 cut(s) 128
NdeII GATC 5 cut(s) 150, 219, 271, 604, 608
NlaIII CATG 2 cut(s) 269, 313
NlaIV GGNNCC 1 cut(s) 124
PagI TCATGA 1 cut(s) 265
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 1 cut(s) 491
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PsiI TTATAA 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 2 cut(s) 122, 329
RsaI GTAC 4 cut(s) 91, 321, 479, 672
RsaNI GTAC 4 cut(s) 90, 320, 478, 671
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 1 cut(s) 490
Sau3AI GATC 5 cut(s) 150, 219, 271, 604, 608
Sau96I GGNCC 2 cut(s) 122, 329
SchI GAGTC 1 cut(s) 619
SetI ASST 9 cut(s) 59, 110, 171, 196, 411, 491, 546, 556, 573
SinI GGWCC 2 cut(s) 122, 329
Sse9I AATT 3 cut(s) 296, 431, 458
SsiI CCGC 1 cut(s) 351
SspMI CTAG 3 cut(s) 144, 275, 315
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 2 cut(s) 59, 573
TaqI TCGA 3 cut(s) 357, 597, 607
TaqII GACCGA 1 cut(s) 317
TasI AATT 3 cut(s) 296, 431, 458
TatI WGTACW 1 cut(s) 319
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TseI GCWGC 1 cut(s) 489
TspDTI ATGAA 1 cut(s) 402
TspGWI ACGGA 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 122, 329
XapI RAATTY 2 cut(s) 296, 431
XbaI TCTAGA 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 3 cut(s) 144, 275, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.