MD15G1422200.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013457
Physical Location & Seq
Forward (+)
52403279 .. 52403992
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1422200.v1.1.491

Sequence Viewer

Length: 714 bp
ATGTCACTGCAGGCTCATCGTCCTCGTGATATTGTCAACGGAATAAGAAGTTGGTGCCAACACTGCCTACGTACCATAAGAATTGCCTCTTCCAACCCACATGCAATTCTATTTCCTTACTGCTACACCGAGTTTAATCACGAGCTCGATGTTTCGAGGCCTAGGCTTATCGATCACCTTACAGGCCTAGAACAACCGTCCCCCGCTGCCCGGTTACTGGATACCTTGTCTCGTTGGCGGACGGAAAACGAAGATGAACGAGTGTCTTGGATCACCTTTGAGGATGCGGTAAATGATGTTACTGGAGGGACCTGGATGGTTGAGAATGACCGTCCAGGTCCCTCTGCAGCAGCAACTTCTGCCATTCGAGCTTTGCCATTGGTGAAAATATCGGAAGGGCAGTTAGCCAACGAGCCAAATTGCCCCGTGTGCAAGGAAGGGTTTGAGGTTGGCGGAGAAGCGAGGGAGATGCCATGCAAGCATGTCTACCATTCCGACTGCATACTCCCTTGGCTTCACATACACAATACATGCCCGGTTTGCCGCTACGAGCTTCAAGCAGCCAGCAGCTGCAGCCCGAATGACGATTACTATGAAGATGAGGAGGAGGAGGAGGAGGAGGTGACAGAAAGCCCAAATATTTGGCGGTGCTGGTGGAATCTCTTCTTAATTTTCTGGCCATTTTCTGCATTGGTTAATTGGACGCAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

238

Amino Acids

27.33

Weight (kDa)

4.91

Isoelectric Point (pI)

62.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 140 - 182 1.2e-13 Ring finger domain
zf-C3HC4_2 PF13923 140 - 181 7.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 140 - 168 3.4e-08 RING-like zinc finger
zf-C3HC4 PF00097 141 - 181 9.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 141 - 179 6.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68180
fragaria_vesca FvH4_5g29600 FvH4_5g29600
malus_domestica MD08G1228300.v1.1 MD15G1422200.v1.1
prunus_persica Prupe.1G567500_v2.0.a1 Prupe.1G567500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0222631
rosa_laevigata RLG00000002042
rosa_multiflora Rmu_sc0001498.1_g000010
rosa_roxburghii Rroxscaffold_3G00236580
rosa_rugosa Rorug07G0211600
rosa_samantha Rh7AG355200 Rh7BG343500 Rh7CG372500 Rh7DG349700
rosa_wichuraiana Rw7G029810 Rw7G030160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 54
AccI GTMKAC 1 cut(s) 486
AciI CCGC 6 cut(s) 204, 238, 287, 453, 544, 646
AclI AACGTT 1 cut(s) 709
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 677
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 2 cut(s) 210, 217
AgsI TTSAA 1 cut(s) 557
AjnI CCWGG 2 cut(s) 311, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 4 cut(s) 145, 371, 553, 570
AluI AGCT 4 cut(s) 145, 371, 553, 570
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 1 cut(s) 278
AlwNI CAGNNNCTG 1 cut(s) 570
AoxI GGCC 3 cut(s) 158, 184, 677
ApeKI GCWGC 7 cut(s) 206, 347, 350, 560, 567, 570, 573
Asp700I GAANNNNTTC 1 cut(s) 662
AspA2I CCTAGG 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 309, 338
AsuC2I CCSGG 2 cut(s) 211, 536
AsuHPI GGTGA 4 cut(s) 167, 265, 394, 634
AvaII GGWCC 2 cut(s) 309, 338
AvrII CCTAGG 1 cut(s) 161
BalI TGGCCA 1 cut(s) 679
BanI GGYRCC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 2 cut(s) 24, 140
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 7 cut(s) 193, 359, 362, 557, 572, 579, 585
BccI CCATC 1 cut(s) 310
BcgI CGANNNNNNTGC 3 cut(s) 33, 451, 485
BciT130I CCWGG 2 cut(s) 313, 336
BciVI GTATCC 1 cut(s) 214
BcnI CCSGG 2 cut(s) 211, 536
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 162, 188
BfmI CTRYAG 3 cut(s) 8, 345, 571
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 8 cut(s) 207, 348, 351, 544, 561, 568, 571, 574
BlnI CCTAGG 1 cut(s) 161
BlsI GCNGC 8 cut(s) 208, 349, 352, 545, 562, 569, 572, 575
Bme1390I CCNGG 4 cut(s) 211, 313, 336, 536
Bme18I GGWCC 2 cut(s) 309, 338
BmgT120I GGNCC 2 cut(s) 309, 338
BmiI GGNNCC 3 cut(s) 56, 310, 340
BmrFI CCNGG 4 cut(s) 211, 313, 336, 536
BmsI GCATC 2 cut(s) 274, 459
BpmI CTGGAG 1 cut(s) 324
BpuMI CCSGG 2 cut(s) 211, 536
Bsa29I ATCGAT 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 161, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 217
Bse1I ACTGG 2 cut(s) 222, 307
BseBI CCWGG 2 cut(s) 313, 336
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 161, 509
BseGI GGATG 2 cut(s) 289, 321
BseLI CCNNNNNNNGG 2 cut(s) 210, 217
BseNI ACTGG 2 cut(s) 222, 307
BseRI GAGGAG 6 cut(s) 617, 620, 623, 626, 629, 632
BseXI GCAGC 7 cut(s) 193, 359, 362, 557, 572, 579, 585
BshFI GGCC 3 cut(s) 160, 186, 679
BshNI GGYRCC 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 2 cut(s) 211, 536
BslFI GGGAC 3 cut(s) 184, 322, 324
BslI CCNNNNNNNGG 2 cut(s) 210, 217
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 3 cut(s) 184, 322, 324
BsnI GGCC 3 cut(s) 160, 186, 679
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 172, 270
BspACI CCGC 6 cut(s) 204, 238, 287, 453, 544, 646
BspANI GGCC 3 cut(s) 160, 186, 679
BspDI ATCGAT 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 56, 310, 340
BspMAI CTGCAG 3 cut(s) 12, 349, 575
BspPI GGATC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 54
BsrI ACTGG 2 cut(s) 222, 307
BssECI CCNNGG 2 cut(s) 161, 509
BssMI GATC 2 cut(s) 172, 270
BssSI CACGAG 2 cut(s) 24, 140
BssT1I CCWWGG 2 cut(s) 161, 509
Bst2BI CACGAG 2 cut(s) 24, 140
Bst2UI CCWGG 2 cut(s) 313, 336
Bst4CI ACNGT 2 cut(s) 198, 332
Bst6I CTCTTC 2 cut(s) 94, 668
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 12, 479, 565
BstF5I GGATG 2 cut(s) 289, 321
BstKTI GATC 2 cut(s) 175, 273
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 2 cut(s) 172, 270
BstMWI GCNNNNNNNGC 7 cut(s) 63, 359, 368, 429, 478, 540, 573
BstNI CCWGG 2 cut(s) 313, 336
BstNSI RCATGY 3 cut(s) 104, 485, 534
BstSCI CCNGG 4 cut(s) 209, 311, 334, 534
BstSFI CTRYAG 3 cut(s) 8, 345, 571
BstSNI TACGTA 1 cut(s) 71
BstV1I GCAGC 7 cut(s) 193, 359, 362, 557, 572, 579, 585
BstXI CCANNNNNNTGG 1 cut(s) 642
Bsu15I ATCGAT 1 cut(s) 171
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 3 cut(s) 160, 186, 679
BsuTUI ATCGAT 1 cut(s) 171
BtsCI GGATG 2 cut(s) 289, 321
BtsI GCAGTG 2 cut(s) 5, 61
BtsIMutI CAGTG 2 cut(s) 5, 61
Cac8I GCNNGC 3 cut(s) 12, 479, 565
CaiI CAGNNNCTG 1 cut(s) 570
Cfr13I GGNCC 2 cut(s) 309, 338
ClaI ATCGAT 1 cut(s) 171
Csp6I GTAC 1 cut(s) 72
CviAII CATG 4 cut(s) 101, 474, 482, 531
CviQI GTAC 1 cut(s) 72
DpnI GATC 2 cut(s) 174, 272
DpnII GATC 2 cut(s) 172, 270
EaeI YGGCCR 1 cut(s) 677
Eam1104I CTCTTC 2 cut(s) 94, 668
EarI CTCTTC 2 cut(s) 94, 668
EciI GGCGGA 2 cut(s) 253, 468
Ecl136II GAGCTC 1 cut(s) 145
Eco105I TACGTA 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 161, 509
Eco147I AGGCCT 2 cut(s) 160, 186
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 309, 338
Eco53kI GAGCTC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 145
EcoO109I RGGNCCY 2 cut(s) 309, 338
EcoRII CCWGG 2 cut(s) 311, 334
EcoT14I CCWWGG 2 cut(s) 161, 509
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 161, 509
FaeI CATG 4 cut(s) 104, 477, 485, 534
FaiI YATR 8 cut(s) 77, 102, 475, 483, 503, 521, 532, 594
FaqI GGGAC 3 cut(s) 184, 322, 324
FatI CATG 4 cut(s) 100, 473, 481, 530
FauI CCCGC 1 cut(s) 211
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 8 cut(s) 207, 348, 351, 544, 561, 568, 571, 574
FokI GGATG 2 cut(s) 296, 328
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 8 cut(s) 207, 348, 351, 544, 561, 568, 571, 574
FspBI CTAG 2 cut(s) 162, 188
GluI GCNGC 8 cut(s) 207, 348, 351, 544, 561, 568, 571, 574
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 3 cut(s) 160, 186, 679
HapII CCGG 2 cut(s) 211, 536
Hin1II CATG 4 cut(s) 104, 477, 485, 534
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 1 cut(s) 658
HpaII CCGG 2 cut(s) 211, 536
HphI GGTGA 4 cut(s) 167, 265, 394, 634
Hpy166II GTNNAC 2 cut(s) 37, 487
Hpy188I TCNGA 2 cut(s) 394, 496
Hpy188III TCNNGA 2 cut(s) 26, 140
Hpy8I GTNNAC 2 cut(s) 37, 487
HpyAV CCTTC 2 cut(s) 389, 431
HpyCH4III ACNGT 2 cut(s) 198, 332
HpyCH4IV ACGT 2 cut(s) 70, 709
HpyCH4V TGCA 8 cut(s) 10, 104, 347, 432, 477, 501, 573, 689
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 359, 368, 429, 478, 540, 573
HpySE526I ACGT 2 cut(s) 70, 709
Hsp92II CATG 4 cut(s) 104, 477, 485, 534
Kzo9I GATC 2 cut(s) 172, 270
Lsp1109I GCAGC 7 cut(s) 193, 359, 362, 557, 572, 579, 585
LweI GCATC 2 cut(s) 274, 459
MaeI CTAG 2 cut(s) 162, 188
MaeII ACGT 2 cut(s) 70, 709
MaeIII GTNAC 4 cut(s) 3, 213, 298, 622
MalI GATC 2 cut(s) 174, 272
MboI GATC 2 cut(s) 172, 270
MboII GAAGA 4 cut(s) 81, 263, 608, 655
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 679
MluCI AATT 5 cut(s) 81, 105, 418, 669, 697
MluNI TGGCCA 1 cut(s) 679
MmeI TCCRAC 2 cut(s) 117, 519
Mox20I TGGCCA 1 cut(s) 679
MroXI GAANNNNTTC 1 cut(s) 662
MscI TGGCCA 1 cut(s) 679
MseI TTAA 4 cut(s) 135, 668, 696, 712
Msp20I TGGCCA 1 cut(s) 679
MspA1I CMGCKG 2 cut(s) 206, 570
MspI CCGG 2 cut(s) 211, 536
MspR9I CCNGG 4 cut(s) 211, 313, 336, 536
MvaI CCWGG 2 cut(s) 313, 336
MwoI GCNNNNNNNGC 7 cut(s) 63, 359, 368, 429, 478, 540, 573
NciI CCSGG 2 cut(s) 211, 536
NdeII GATC 2 cut(s) 172, 270
NlaIII CATG 4 cut(s) 104, 477, 485, 534
NlaIV GGNNCC 3 cut(s) 56, 310, 340
NmuCI GTSAC 2 cut(s) 3, 622
NspI RCATGY 3 cut(s) 104, 485, 534
PceI AGGCCT 2 cut(s) 160, 186
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 1 cut(s) 658
PkrI GCNGC 8 cut(s) 208, 349, 352, 545, 562, 569, 572, 575
Ppu21I YACGTR 1 cut(s) 71
PpuMI RGGWCCY 2 cut(s) 309, 338
Psp124BI GAGCTC 1 cut(s) 147
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 2 cut(s) 309, 338
Psp6I CCWGG 2 cut(s) 311, 334
PspGI CCWGG 2 cut(s) 311, 334
PspN4I GGNNCC 3 cut(s) 56, 310, 340
PspPI GGNCC 2 cut(s) 309, 338
PspPPI RGGWCCY 2 cut(s) 309, 338
PstI CTGCAG 3 cut(s) 12, 349, 575
PstNI CAGNNNCTG 1 cut(s) 570
PvuII CAGCTG 1 cut(s) 570
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 4 cut(s) 135, 668, 696, 712
SatI GCNGC 8 cut(s) 207, 348, 351, 544, 561, 568, 571, 574
Sau3AI GATC 2 cut(s) 172, 270
Sau96I GGNCC 2 cut(s) 309, 338
ScrFI CCNGG 4 cut(s) 211, 313, 336, 536
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 2 cut(s) 274, 459
SfcI CTRYAG 3 cut(s) 8, 345, 571
SinI GGWCC 2 cut(s) 309, 338
SnaBI TACGTA 1 cut(s) 71
Sse9I AATT 5 cut(s) 81, 105, 418, 669, 697
SseBI AGGCCT 2 cut(s) 160, 186
SsiI CCGC 6 cut(s) 204, 238, 287, 453, 544, 646
SspI AATATT 1 cut(s) 640
SspMI CTAG 2 cut(s) 162, 188
SstI GAGCTC 1 cut(s) 147
StuI AGGCCT 2 cut(s) 160, 186
StyD4I CCNGG 4 cut(s) 209, 311, 334, 534
StyI CCWWGG 2 cut(s) 161, 509
TaaI ACNGT 2 cut(s) 198, 332
TaiI ACGT 2 cut(s) 73, 712
TaqI TCGA 4 cut(s) 147, 155, 171, 367
TasI AATT 5 cut(s) 81, 105, 418, 669, 697
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 1 cut(s) 658
Tru1I TTAA 4 cut(s) 135, 668, 696, 712
Tru9I TTAA 4 cut(s) 135, 668, 696, 712
TscAI CASTG 2 cut(s) 12, 68
TseFI GTSAC 2 cut(s) 3, 622
TseI GCWGC 7 cut(s) 206, 347, 350, 560, 567, 570, 573
Tsp45I GTSAC 2 cut(s) 3, 622
TspDTI ATGAA 2 cut(s) 270, 609
TspGWI ACGGA 2 cut(s) 54, 257
TspRI CASTG 2 cut(s) 12, 68
VpaK11BI GGWCC 2 cut(s) 309, 338
XceI RCATGY 3 cut(s) 104, 485, 534
XmaJI CCTAGG 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 2 cut(s) 162, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.