MD08G1228300.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013457
Physical Location & Seq
Forward (+)
29451768 .. 29453130
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1228300.v1.1.491

Sequence Viewer

Length: 936 bp
ATGTCACTGCAGGCTCATCGTCCTCGTGTTACTGTCAACGGAATAAGAAGAATGAGAACACACCGTTACTTCTGGTGCCAACATTGCCAACGTACCATAAGGATTGCCTCTTCCAACCCATATACAATTCTATGTCCCTACTGCTCCACCGAGTTCAACCATGAGCTCGATGTAGCAAATCCTAGGCTTGCCGATCATCTTATTACAGACCTAGAACAACCACCCTCCCCCGCTGCCCGGTTACTGGAGTCCTTGTCTCTCGCCCTCGATTCAAGGCTTCCAAGAGCTCGATGGCAGACGGAAAACGAAGACGAACGATTGTCTTGGATCACCCTCCGATTTGATAGACCACCTCGCACTCCGCGACTCCAAGTTTCTGCGTCATCAGAAAACCTTGTGCCTCGACCAAATTATAATCGCGAGGATGGCGCTTTTGAGGATGCGGTAAGAGATGTTACTGGAGAGACTTGGACTGTTGAGAATGACCGTCCAGGTCCCCCTCCGGCAGCAACTTCTGCCATTCGAGCTTTGCCATTGGTGACAGTCTCGGAAGGGCAGTTAGCGAACGAGCCCAGTTGCCCCGTGTGCAAGGAAGCGTTCGAGGTTGGTGGGGAAGTGAGGGAGATGCCATGCAAGCACGTCTACCATTCTGATTGCATACTCCCTTGGCTTCACATCCACAATACTTGTCCGGTTTGCCGCTACGAGCTTCGAACAGCCGGCAGCTGTAACCCTAATGACGATTACTATGGAGATGAGGAGGAGGTGACTGAAAGCCCAGATATTTGGGGGTGGTGGAATCACTTCATATCCTTCTGGCCATTTTCTGCATTGGTTAATTGGACGCAACGTTACTTGGATAACCAACAACGTACTGGAGACAGAACCTGGTGGTCCCTCTTGGATGATTCTATAGGACATTTGTTCCTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

312

Amino Acids

35.95

Weight (kDa)

5.48

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 24 - 55 5.7e-06 zinc-ribbon
zf-RING_2 PF13639 192 - 234 6.3e-14 Ring finger domain
zf-C3HC4_2 PF13923 193 - 233 3.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 193 - 220 2.2e-08 RING-like zinc finger
zf-C3HC4 PF00097 193 - 233 6.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 193 - 231 1.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68180
fragaria_vesca FvH4_5g29600 FvH4_5g29600
malus_domestica MD08G1228300.v1.1 MD15G1422200.v1.1
prunus_persica Prupe.1G567500_v2.0.a1 Prupe.1G567500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0222631
rosa_laevigata RLG00000002042
rosa_multiflora Rmu_sc0001498.1_g000010
rosa_roxburghii Rroxscaffold_3G00236580
rosa_rugosa Rorug07G0211600
rosa_samantha Rh7AG355200 Rh7BG343500 Rh7CG372500 Rh7DG349700
rosa_wichuraiana Rw7G029810 Rw7G030160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 642
AccII CGCG 2 cut(s) 364, 420
AciI CCGC 4 cut(s) 231, 362, 443, 700
AclI AACGTT 1 cut(s) 850
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 818
AfaI GTAC 2 cut(s) 94, 874
AfiI CCNNNNNNNGG 2 cut(s) 237, 244
AgsI TTSAA 2 cut(s) 157, 273
AjiI CACGTC 1 cut(s) 640
AjnI CCWGG 2 cut(s) 490, 887
AluBI AGCT 5 cut(s) 166, 287, 527, 709, 726
AluI AGCT 5 cut(s) 166, 287, 527, 709, 726
Alw21I GWGCWC 2 cut(s) 168, 289
Alw26I GTCTC 4 cut(s) 261, 458, 550, 873
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 888
AoxI GGCC 1 cut(s) 818
ApeKI GCWGC 3 cut(s) 233, 506, 723
Asp700I GAANNNNTTC 1 cut(s) 803
AspA2I CCTAGG 1 cut(s) 182
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 2 cut(s) 494, 894
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 322, 550, 778
AsuII TTCGAA 1 cut(s) 712
AvaII GGWCC 2 cut(s) 494, 894
AvrII CCTAGG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 820
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 3 cut(s) 168, 289, 573
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 168, 289
BbvI GCAGC 3 cut(s) 220, 518, 735
BccI CCATC 2 cut(s) 285, 419
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 2 cut(s) 492, 889
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 4 cut(s) 261, 458, 550, 873
BfaI CTAG 2 cut(s) 183, 212
BfmI CTRYAG 2 cut(s) 8, 912
BfoI RGCGCY 1 cut(s) 432
BisI GCNGC 4 cut(s) 234, 507, 700, 724
BlnI CCTAGG 1 cut(s) 182
BlsI GCNGC 4 cut(s) 235, 508, 701, 725
Bme1390I CCNGG 3 cut(s) 238, 492, 889
Bme18I GGWCC 2 cut(s) 494, 894
BmgBI CACGTC 1 cut(s) 640
BmgT120I GGNCC 2 cut(s) 494, 894
BmiI GGNNCC 3 cut(s) 77, 496, 896
BmrFI CCNGG 3 cut(s) 238, 492, 889
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 2 cut(s) 430, 615
BmuI ACTGGG 1 cut(s) 567
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 3 cut(s) 266, 480, 897
Bpu14I TTCGAA 1 cut(s) 712
BpuMI CCSGG 1 cut(s) 238
BsaJI CCNNGG 2 cut(s) 182, 665
BsaWI WCCGGW 1 cut(s) 691
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 244
Bse118I RCCGGY 1 cut(s) 719
Bse1I ACTGG 4 cut(s) 249, 463, 573, 880
Bse3DI GCAATG 1 cut(s) 82
BseBI CCWGG 2 cut(s) 492, 889
BseDI CCNNGG 2 cut(s) 182, 665
BseGI GGATG 4 cut(s) 430, 445, 675, 910
BseLI CCNNNNNNNGG 2 cut(s) 237, 244
BseMI GCAATG 1 cut(s) 82
BseNI ACTGG 4 cut(s) 249, 463, 573, 880
BseRI GAGGAG 2 cut(s) 773, 776
BseXI GCAGC 3 cut(s) 220, 518, 735
Bsh1236I CGCG 2 cut(s) 364, 420
BshFI GGCC 1 cut(s) 820
BshNI GGYRCC 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 168, 289
BsiSI CCGG 4 cut(s) 238, 503, 692, 720
BslFI GGGAC 3 cut(s) 120, 480, 880
BslI CCNNNNNNNGG 2 cut(s) 237, 244
BsmAI GTCTC 4 cut(s) 261, 458, 550, 873
BsmFI GGGAC 3 cut(s) 120, 480, 880
BsnI GGCC 1 cut(s) 820
Bsp119I TTCGAA 1 cut(s) 712
Bsp1286I GDGCHC 3 cut(s) 168, 289, 573
Bsp143I GATC 2 cut(s) 193, 327
Bsp68I TCGCGA 1 cut(s) 420
BspACI CCGC 4 cut(s) 231, 362, 443, 700
BspANI GGCC 1 cut(s) 820
BspFNI CGCG 2 cut(s) 364, 420
BspLI GGNNCC 3 cut(s) 77, 496, 896
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 1 cut(s) 335
BspT104I TTCGAA 1 cut(s) 712
BspT107I GGYRCC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 719
BsrI ACTGG 4 cut(s) 249, 463, 573, 880
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 2 cut(s) 182, 665
BssMI GATC 2 cut(s) 193, 327
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 182, 665
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 492, 889
Bst4CI ACNGT 5 cut(s) 34, 65, 475, 488, 544
Bst6I CTCTTC 1 cut(s) 115
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 712
BstC8I GCNNGC 4 cut(s) 12, 189, 635, 721
BstF5I GGATG 4 cut(s) 430, 445, 675, 910
BstFNI CGCG 2 cut(s) 364, 420
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 2 cut(s) 196, 330
BstMAI GTCTC 4 cut(s) 261, 458, 550, 873
BstMBI GATC 2 cut(s) 193, 327
BstMWI GCNNNNNNNGC 6 cut(s) 84, 426, 515, 524, 585, 634
BstNI CCWGG 2 cut(s) 492, 889
BstSCI CCNGG 3 cut(s) 236, 490, 887
BstSFI CTRYAG 2 cut(s) 8, 912
BstUI CGCG 2 cut(s) 364, 420
BstV1I GCAGC 3 cut(s) 220, 518, 735
BstV2I GAAGAC 1 cut(s) 315
BstXI CCANNNNNNTGG 1 cut(s) 786
BsuRI GGCC 1 cut(s) 820
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 4 cut(s) 430, 445, 675, 910
BtsI GCAGTG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 5
BtuMI TCGCGA 1 cut(s) 420
Cac8I GCNNGC 4 cut(s) 12, 189, 635, 721
CaiI CAGNNNCTG 1 cut(s) 888
CfoI GCGC 1 cut(s) 431
Cfr10I RCCGGY 1 cut(s) 719
Cfr13I GGNCC 2 cut(s) 494, 894
CseI GACGC 2 cut(s) 369, 853
CsiI ACCWGGT 1 cut(s) 887
Csp6I GTAC 2 cut(s) 93, 873
CspCI CAANNNNNGTGG 2 cut(s) 668, 703
CviAII CATG 2 cut(s) 161, 630
CviQI GTAC 2 cut(s) 93, 873
DpnI GATC 2 cut(s) 195, 329
DpnII GATC 2 cut(s) 193, 327
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Ecl136II GAGCTC 2 cut(s) 166, 287
Eco130I CCWWGG 2 cut(s) 182, 665
Eco24I GRGCYC 3 cut(s) 168, 289, 573
Eco47I GGWCC 2 cut(s) 494, 894
Eco53kI GAGCTC 2 cut(s) 166, 287
EcoICRI GAGCTC 2 cut(s) 166, 287
EcoO109I RGGNCCY 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 490, 887
EcoT14I CCWWGG 2 cut(s) 182, 665
EcoT38I GRGCYC 3 cut(s) 168, 289, 573
ErhI CCWWGG 2 cut(s) 182, 665
FaeI CATG 2 cut(s) 164, 633
FaqI GGGAC 3 cut(s) 120, 480, 880
FatI CATG 2 cut(s) 160, 629
FauI CCCGC 1 cut(s) 238
FblI GTMKAC 1 cut(s) 642
Fnu4HI GCNGC 4 cut(s) 234, 507, 700, 724
FokI GGATG 4 cut(s) 437, 452, 662, 917
FriOI GRGCYC 3 cut(s) 168, 289, 573
Fsp4HI GCNGC 4 cut(s) 234, 507, 700, 724
FspBI CTAG 2 cut(s) 183, 212
GlaI GCGC 1 cut(s) 430
GluI GCNGC 4 cut(s) 234, 507, 700, 724
GsuI CTGGAG 3 cut(s) 266, 480, 897
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 1 cut(s) 820
HapII CCGG 4 cut(s) 238, 503, 692, 720
HgaI GACGC 2 cut(s) 369, 853
HhaI GCGC 1 cut(s) 431
Hin1II CATG 2 cut(s) 164, 633
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 5 cut(s) 248, 269, 366, 799, 908
HpaII CCGG 4 cut(s) 238, 503, 692, 720
HphI GGTGA 3 cut(s) 322, 550, 778
Hpy166II GTNNAC 2 cut(s) 37, 643
Hpy188I TCNGA 4 cut(s) 338, 388, 550, 652
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 2 cut(s) 37, 643
HpyAV CCTTC 2 cut(s) 545, 823
HpyCH4III ACNGT 5 cut(s) 34, 65, 475, 488, 544
HpyCH4IV ACGT 4 cut(s) 91, 639, 850, 871
HpyCH4V TGCA 5 cut(s) 10, 588, 633, 657, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 426, 515, 524, 585, 634
HpySE526I ACGT 4 cut(s) 91, 639, 850, 871
Hsp92II CATG 2 cut(s) 164, 633
HspAI GCGC 1 cut(s) 429
KroI GCCGGC 1 cut(s) 719
KroNI GCCGGC 1 cut(s) 721
Kzo9I GATC 2 cut(s) 193, 327
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 3 cut(s) 220, 518, 735
LweI GCATC 2 cut(s) 430, 615
MabI ACCWGGT 1 cut(s) 887
MaeI CTAG 2 cut(s) 183, 212
MaeII ACGT 4 cut(s) 91, 639, 850, 871
MaeIII GTNAC 9 cut(s) 3, 28, 65, 240, 454, 538, 728, 766, 851
MalI GATC 2 cut(s) 195, 329
MboI GATC 2 cut(s) 193, 327
MboII GAAGA 3 cut(s) 60, 102, 320
MhlI GDGCHC 3 cut(s) 168, 289, 573
MlsI TGGCCA 1 cut(s) 820
MluCI AATT 4 cut(s) 126, 409, 838, 931
MluNI TGGCCA 1 cut(s) 820
MlyI GAGTC 2 cut(s) 257, 360
MmeI TCCRAC 1 cut(s) 138
Mox20I TGGCCA 1 cut(s) 820
MroNI GCCGGC 1 cut(s) 719
MroXI GAANNNNTTC 1 cut(s) 803
MscI TGGCCA 1 cut(s) 820
MseI TTAA 2 cut(s) 837, 930
Msp20I TGGCCA 1 cut(s) 820
MspA1I CMGCKG 2 cut(s) 233, 726
MspI CCGG 4 cut(s) 238, 503, 692, 720
MspR9I CCNGG 3 cut(s) 238, 492, 889
MvaI CCWGG 2 cut(s) 492, 889
MvnI CGCG 2 cut(s) 364, 420
MwoI GCNNNNNNNGC 6 cut(s) 84, 426, 515, 524, 585, 634
NaeI GCCGGC 1 cut(s) 721
NciI CCSGG 1 cut(s) 238
NdeII GATC 2 cut(s) 193, 327
NgoMIV GCCGGC 1 cut(s) 719
NlaIII CATG 2 cut(s) 164, 633
NlaIV GGNNCC 3 cut(s) 77, 496, 896
NmuCI GTSAC 3 cut(s) 3, 538, 766
NruI TCGCGA 1 cut(s) 420
NspV TTCGAA 1 cut(s) 712
PcsI WCGNNNNNNNCGW 1 cut(s) 361
PdiI GCCGGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 803
PfeI GAWTC 3 cut(s) 269, 799, 908
PkrI GCNGC 4 cut(s) 235, 508, 701, 725
PleI GAGTC 2 cut(s) 256, 360
PpsI GAGTC 2 cut(s) 256, 360
PpuMI RGGWCCY 1 cut(s) 494
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 2 cut(s) 168, 289
Psp1406I AACGTT 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 490, 887
PspGI CCWGG 2 cut(s) 490, 887
PspN4I GGNNCC 3 cut(s) 77, 496, 896
PspPI GGNCC 2 cut(s) 494, 894
PspPPI RGGWCCY 1 cut(s) 494
PstI CTGCAG 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 888
PvuII CAGCTG 1 cut(s) 726
RruI TCGCGA 1 cut(s) 420
RsaI GTAC 2 cut(s) 94, 874
RsaNI GTAC 2 cut(s) 93, 873
SacI GAGCTC 2 cut(s) 168, 289
SaqAI TTAA 2 cut(s) 837, 930
SatI GCNGC 4 cut(s) 234, 507, 700, 724
Sau3AI GATC 2 cut(s) 193, 327
Sau96I GGNCC 2 cut(s) 494, 894
SchI GAGTC 2 cut(s) 257, 360
ScrFI CCNGG 3 cut(s) 238, 492, 889
SduI GDGCHC 3 cut(s) 168, 289, 573
SexAI ACCWGGT 1 cut(s) 887
SfaNI GCATC 2 cut(s) 430, 615
SfcI CTRYAG 2 cut(s) 8, 912
SfuI TTCGAA 1 cut(s) 712
SinI GGWCC 2 cut(s) 494, 894
Sse9I AATT 4 cut(s) 126, 409, 838, 931
SsiI CCGC 4 cut(s) 231, 362, 443, 700
SspMI CTAG 2 cut(s) 183, 212
SstI GAGCTC 2 cut(s) 168, 289
StyD4I CCNGG 3 cut(s) 236, 490, 887
StyI CCWWGG 2 cut(s) 182, 665
TaaI ACNGT 5 cut(s) 34, 65, 475, 488, 544
TaiI ACGT 4 cut(s) 94, 642, 853, 874
TaqI TCGA 7 cut(s) 168, 267, 289, 403, 523, 600, 712
TasI AATT 4 cut(s) 126, 409, 838, 931
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 3 cut(s) 269, 799, 908
Tru1I TTAA 2 cut(s) 837, 930
Tru9I TTAA 2 cut(s) 837, 930
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 3 cut(s) 3, 538, 766
TseI GCWGC 3 cut(s) 233, 506, 723
Tsp45I GTSAC 3 cut(s) 3, 538, 766
TspDTI ATGAA 1 cut(s) 796
TspGWI ACGGA 2 cut(s) 54, 314
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 494, 894
XcmI CCANNNNNNNNNTGG 2 cut(s) 288, 872
XmaJI CCTAGG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 642
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 2 cut(s) 183, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.