FvH4_5g29600

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013457
Physical Location & Seq
Reverse (-)
20540733 .. 20542932
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29600.t1

Sequence Viewer

Length: 915 bp
ATGTCGTCGTCACTGGTTCGTCCTCGTCCTCGTGTTATGGTCAATGGAATAAGAAGAATGAGAACCTTCCGTTTCTTCTGGTGCCGCCATTGCCAACATACTGTAAGAATTGCCTCTTCTAACCCATATGGACTTCAATGTCACTATTGCTCCAATGAGCTCAATCATGAGCTCGATGTATTAAGGCCTGCAGCCTCCCTCAATAACCACCTTCATCAAGCCCTAGAACCTTCTCCCACTGCCCGGTTACTTCAAAACCTTGCTCTAGCCCTCGATCCTCCCCCTCCGAGGCACCACGGACGACTTCGATGGCAAATCGAAACCGACAATGAGCCTGTTGATCATCCGCGGTCTTGGATCACCCTCCAGTTCGACAGACCGCCAAGACTTGTAGTCCCTGTGCCACAAAATGCCACCCTCGACGAGGATCATACTAATAACGCTACTACTTTCGAGGAAGCAACAAATGAGGTTCAAAGTGAGCGTCGAGGGCCGCCGCCAGCACCCGGTTGGGCAATCGAAGGATTGCCGACGGTGAAAGTCACGGAAGAGGGGTTGGTGAAGGAACCTGGTTGCCCGGTTTGCAAAGAAGTGTTTGTGGTTGGTGGAGAAGTGAGAGAAATGCCATGCAAACATGTGTATCATTCTGATTGCATTGTCCCTTGGCTCAGCTTACACAATACTTGTCCAGTTTGCCGATATGAGCTTATTAACAATGGGATCGATGATGATCGAAATCCTGAAAATTATTACGCAGAGGAGGAGGAAGAAGCGGTGACGTCACGATATTGGTGGTGGAATCAGTTCCTTTCCTTGTGGCCATTTCGTGCATTAGTTCATTGGAGGCAAGGTTACCTTGATAACCAAGCAAGGCCTCAAGGTAGAACCTGGTGGTCCTCTTGGATTATTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

305

Amino Acids

35.38

Weight (kDa)

6.27

Isoelectric Point (pI)

60.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 192 - 233 1.4e-12 Ring finger domain
zf-C3HC4_2 PF13923 192 - 232 4.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 192 - 219 1.7e-07 RING-like zinc finger
zf-C3HC4 PF00097 192 - 232 6.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 192 - 230 4.9e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68180
fragaria_vesca FvH4_5g29600 FvH4_5g29600
malus_domestica MD08G1228300.v1.1 MD15G1422200.v1.1
prunus_persica Prupe.1G567500_v2.0.a1 Prupe.1G567500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0222631
rosa_laevigata RLG00000002042
rosa_multiflora Rmu_sc0001498.1_g000010
rosa_roxburghii Rroxscaffold_3G00236580
rosa_rugosa Rorug07G0211600
rosa_samantha Rh7AG355200 Rh7BG343500 Rh7CG372500 Rh7DG349700
rosa_wichuraiana Rw7G029810 Rw7G030160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 138
AatII GACGTC 1 cut(s) 782
AccB1I GGYRCC 2 cut(s) 81, 291
AccII CGCG 1 cut(s) 349
AciI CCGC 7 cut(s) 85, 347, 349, 380, 494, 497, 773
AclWI GGATC 4 cut(s) 269, 365, 435, 728
AcoI YGGCCR 1 cut(s) 818
AcyI GRCGYC 1 cut(s) 779
AfiI CCNNNNNNNGG 4 cut(s) 243, 288, 424, 506
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 3 cut(s) 137, 254, 476
AhdI GACNNNNNGTC 1 cut(s) 392
AjnI CCWGG 2 cut(s) 568, 887
AluBI AGCT 4 cut(s) 160, 172, 672, 706
AluI AGCT 4 cut(s) 160, 172, 672, 706
Alw21I GWGCWC 2 cut(s) 162, 174
AlwI GGATC 4 cut(s) 269, 365, 435, 728
AoxI GGCC 4 cut(s) 185, 491, 818, 872
ApeKI GCWGC 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 469, 501
Asp700I GAANNNNTTC 1 cut(s) 803
AspS9I GGNCC 2 cut(s) 491, 894
AsuC2I CCSGG 3 cut(s) 244, 507, 578
AsuHPI GGTGA 4 cut(s) 352, 547, 571, 787
AvaII GGWCC 1 cut(s) 894
BaeI ACNNNNGTAYC 2 cut(s) 623, 656
BalI TGGCCA 1 cut(s) 820
BanI GGYRCC 2 cut(s) 81, 291
BanII GRGCYC 2 cut(s) 162, 174
BauI CACGAG 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 162, 174
BbvI GCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 303
BciT130I CCWGG 2 cut(s) 570, 889
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 3 cut(s) 244, 507, 578
BfaI CTAG 2 cut(s) 224, 266
BfmI CTRYAG 2 cut(s) 189, 911
BisI GCNGC 4 cut(s) 85, 192, 494, 497
BlpI GCTNAGC 1 cut(s) 668
BlsI GCNGC 4 cut(s) 86, 193, 495, 498
Bme1390I CCNGG 5 cut(s) 244, 507, 570, 578, 889
Bme18I GGWCC 1 cut(s) 894
BmeRI GACNNNNNGTC 1 cut(s) 392
BmgT120I GGNCC 2 cut(s) 491, 894
BmiI GGNNCC 3 cut(s) 83, 293, 567
BmrFI CCNGG 5 cut(s) 244, 507, 570, 578, 889
BpmI CTGGAG 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 861
BpuMI CCSGG 3 cut(s) 244, 507, 578
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 2 cut(s) 729, 735
BsaHI GRCGYC 1 cut(s) 779
BsaJI CCNNGG 4 cut(s) 287, 295, 347, 662
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 835, 865
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 288, 424, 506
Bse1I ACTGG 3 cut(s) 18, 367, 689
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 2 cut(s) 729, 735
BseBI CCWGG 2 cut(s) 570, 889
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 4 cut(s) 287, 295, 347, 662
BseGI GGATG 1 cut(s) 343
BseJI GATNNNNATC 2 cut(s) 729, 735
BseLI CCNNNNNNNGG 4 cut(s) 243, 288, 424, 506
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 682
BseNI ACTGG 3 cut(s) 18, 367, 689
BseRI GAGGAG 2 cut(s) 773, 776
BseXI GCAGC 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 4 cut(s) 187, 493, 820, 874
BshNI GGYRCC 2 cut(s) 81, 291
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 2 cut(s) 162, 174
BsiSI CCGG 3 cut(s) 244, 507, 578
BslFI GGGAC 2 cut(s) 380, 644
BslI CCNNNNNNNGG 4 cut(s) 243, 288, 424, 506
BsmFI GGGAC 2 cut(s) 380, 644
BsnI GGCC 4 cut(s) 187, 493, 820, 874
Bsp1286I GDGCHC 2 cut(s) 162, 174
Bsp143I GATC 6 cut(s) 274, 340, 357, 427, 720, 730
Bsp1720I GCTNAGC 1 cut(s) 668
BspACI CCGC 7 cut(s) 85, 347, 349, 380, 494, 497, 773
BspANI GGCC 4 cut(s) 187, 493, 820, 874
BspCNI CTCAG 1 cut(s) 681
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 83, 293, 567
BspMAI CTGCAG 1 cut(s) 193
BspPI GGATC 4 cut(s) 269, 365, 435, 728
BspT107I GGYRCC 2 cut(s) 81, 291
BsrDI GCAATG 1 cut(s) 88
BsrI ACTGG 3 cut(s) 18, 367, 689
BssECI CCNNGG 4 cut(s) 287, 295, 347, 662
BssMI GATC 6 cut(s) 274, 340, 357, 427, 720, 730
BssNI GRCGYC 1 cut(s) 779
BssSI CACGAG 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 570, 889
Bst4CI ACNGT 2 cut(s) 103, 535
Bst6I CTCTTC 2 cut(s) 121, 543
BstACI GRCGYC 1 cut(s) 779
BstC8I GCNNGC 2 cut(s) 189, 501
BstDEI CTNAG 1 cut(s) 668
BstDSI CCRYGG 2 cut(s) 295, 347
BstEII GGTNACC 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 6 cut(s) 277, 343, 360, 430, 723, 733
BstMBI GATC 6 cut(s) 274, 340, 357, 427, 720, 730
BstMWI GCNNNNNNNGC 3 cut(s) 90, 490, 582
BstNI CCWGG 2 cut(s) 570, 889
BstNSI RCATGY 1 cut(s) 638
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 5 cut(s) 242, 505, 568, 576, 887
BstSFI CTRYAG 2 cut(s) 189, 911
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 203
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 4 cut(s) 187, 493, 820, 874
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 2 cut(s) 295, 347
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 11, 237
Cac8I GCNNGC 2 cut(s) 189, 501
CciI TCATGA 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 491, 894
Cfr42I CCGCGG 1 cut(s) 350
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 473
CsiI ACCWGGT 2 cut(s) 568, 887
CviAII CATG 3 cut(s) 167, 627, 635
DdeI CTNAG 1 cut(s) 668
DpnI GATC 6 cut(s) 276, 342, 359, 429, 722, 732
DpnII GATC 6 cut(s) 274, 340, 357, 427, 720, 730
DrdI GACNNNNNNGTC 1 cut(s) 138
DriI GACNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 2 cut(s) 121, 543
Eam1105I GACNNNNNGTC 1 cut(s) 392
EarI CTCTTC 2 cut(s) 121, 543
Ecl136II GAGCTC 2 cut(s) 160, 172
Eco130I CCWWGG 1 cut(s) 662
Eco147I AGGCCT 2 cut(s) 187, 874
Eco24I GRGCYC 2 cut(s) 162, 174
Eco47I GGWCC 1 cut(s) 894
Eco53kI GAGCTC 2 cut(s) 160, 172
Eco91I GGTNACC 1 cut(s) 851
EcoICRI GAGCTC 2 cut(s) 160, 172
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 851
EcoRII CCWGG 2 cut(s) 568, 887
EcoT14I CCWWGG 1 cut(s) 662
EcoT38I GRGCYC 2 cut(s) 162, 174
ErhI CCWWGG 1 cut(s) 662
FaeI CATG 3 cut(s) 170, 630, 638
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 2 cut(s) 380, 644
FatI CATG 3 cut(s) 166, 626, 634
FauNDI CATATG 1 cut(s) 127
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 85, 192, 494, 497
FokI GGATG 1 cut(s) 330
FriOI GRGCYC 2 cut(s) 162, 174
Fsp4HI GCNGC 4 cut(s) 85, 192, 494, 497
FspBI CTAG 2 cut(s) 224, 266
GluI GCNGC 4 cut(s) 85, 192, 494, 497
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 4 cut(s) 187, 493, 820, 874
HapII CCGG 3 cut(s) 244, 507, 578
HgaI GACGC 1 cut(s) 473
Hin1I GRCGYC 1 cut(s) 779
Hin1II CATG 3 cut(s) 170, 630, 638
HinfI GANTC 1 cut(s) 799
HpaII CCGG 3 cut(s) 244, 507, 578
HphI GGTGA 4 cut(s) 352, 547, 571, 787
Hpy188I TCNGA 2 cut(s) 288, 649
Hpy188III TCNNGA 3 cut(s) 167, 740, 783
Hpy99I CGWCG 4 cut(s) 10, 425, 489, 535
HpyAV CCTTC 5 cut(s) 76, 221, 240, 515, 556
HpyCH4III ACNGT 2 cut(s) 103, 535
HpyCH4IV ACGT 1 cut(s) 779
HpyCH4V TGCA 5 cut(s) 191, 585, 630, 654, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 490, 582
HpyF3I CTNAG 1 cut(s) 668
HpySE526I ACGT 1 cut(s) 779
Hsp92I GRCGYC 1 cut(s) 779
Hsp92II CATG 3 cut(s) 170, 630, 638
Ksp22I TGATCA 1 cut(s) 340
KspI CCGCGG 1 cut(s) 350
Kzo9I GATC 6 cut(s) 274, 340, 357, 427, 720, 730
LmnI GCTCC 1 cut(s) 155
Lsp1109I GCAGC 1 cut(s) 203
MabI ACCWGGT 2 cut(s) 568, 887
MaeI CTAG 2 cut(s) 224, 266
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 7 cut(s) 9, 140, 246, 541, 775, 780, 851
MalI GATC 6 cut(s) 276, 342, 359, 429, 722, 732
MboI GATC 6 cut(s) 274, 340, 357, 427, 720, 730
MboII GAAGA 5 cut(s) 66, 67, 108, 560, 779
MhlI GDGCHC 2 cut(s) 162, 174
MlsI TGGCCA 1 cut(s) 820
MluCI AATT 2 cut(s) 108, 745
MluNI TGGCCA 1 cut(s) 820
Mox20I TGGCCA 1 cut(s) 820
MroXI GAANNNNTTC 1 cut(s) 803
MscI TGGCCA 1 cut(s) 820
MseI TTAA 2 cut(s) 182, 711
Msp20I TGGCCA 1 cut(s) 820
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 3 cut(s) 244, 507, 578
MspR9I CCNGG 5 cut(s) 244, 507, 570, 578, 889
MvaI CCWGG 2 cut(s) 570, 889
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 90, 490, 582
NciI CCSGG 3 cut(s) 244, 507, 578
NdeI CATATG 1 cut(s) 127
NdeII GATC 6 cut(s) 274, 340, 357, 427, 720, 730
NlaIII CATG 3 cut(s) 170, 630, 638
NlaIV GGNNCC 3 cut(s) 83, 293, 567
NmuCI GTSAC 5 cut(s) 9, 140, 541, 775, 780
NspI RCATGY 1 cut(s) 638
PagI TCATGA 1 cut(s) 166
PceI AGGCCT 2 cut(s) 187, 874
PciI ACATGT 1 cut(s) 634
PdmI GAANNNNTTC 1 cut(s) 803
PfeI GAWTC 1 cut(s) 799
PkrI GCNGC 4 cut(s) 86, 193, 495, 498
PscI ACATGT 1 cut(s) 634
Psp124BI GAGCTC 2 cut(s) 162, 174
Psp6I CCWGG 2 cut(s) 568, 887
PspEI GGTNACC 1 cut(s) 851
PspGI CCWGG 2 cut(s) 568, 887
PspN4I GGNNCC 3 cut(s) 83, 293, 567
PspPI GGNCC 2 cut(s) 491, 894
PstI CTGCAG 1 cut(s) 193
SacI GAGCTC 2 cut(s) 162, 174
SacII CCGCGG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 182, 711
SatI GCNGC 4 cut(s) 85, 192, 494, 497
Sau3AI GATC 6 cut(s) 274, 340, 357, 427, 720, 730
Sau96I GGNCC 2 cut(s) 491, 894
ScrFI CCNGG 5 cut(s) 244, 507, 570, 578, 889
SduI GDGCHC 2 cut(s) 162, 174
SexAI ACCWGGT 2 cut(s) 568, 887
SfcI CTRYAG 2 cut(s) 189, 911
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SinI GGWCC 1 cut(s) 894
SmlI CTYRAG 1 cut(s) 876
SmoI CTYRAG 1 cut(s) 876
Sse9I AATT 2 cut(s) 108, 745
SseBI AGGCCT 2 cut(s) 187, 874
SsiI CCGC 7 cut(s) 85, 347, 349, 380, 494, 497, 773
SspMI CTAG 2 cut(s) 224, 266
SstI GAGCTC 2 cut(s) 162, 174
StuI AGGCCT 2 cut(s) 187, 874
StyD4I CCNGG 5 cut(s) 242, 505, 568, 576, 887
StyI CCWWGG 1 cut(s) 662
TaaI ACNGT 2 cut(s) 103, 535
TaiI ACGT 1 cut(s) 782
TasI AATT 2 cut(s) 108, 745
TauI GCSGC 3 cut(s) 87, 496, 499
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 2 cut(s) 182, 711
Tru9I TTAA 2 cut(s) 182, 711
TscAI CASTG 2 cut(s) 18, 244
TseFI GTSAC 5 cut(s) 9, 140, 541, 775, 780
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 5 cut(s) 9, 140, 541, 775, 780
TspDTI ATGAA 2 cut(s) 203, 827
TspGWI ACGGA 3 cut(s) 59, 312, 560
TspRI CASTG 2 cut(s) 18, 244
VpaK11BI GGWCC 1 cut(s) 894
XagI CCTNNNNNAGG 1 cut(s) 422
XceI RCATGY 1 cut(s) 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 2 cut(s) 224, 266
ZraI GACGTC 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.