AT1G68620

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014973
Physical Location & Seq
Forward (+)
25765718 .. 25767240
1523 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68620.1

Sequence Viewer

Length: 1011 bp
ATGGGAGGCACAAAATTGACCCATGTGACCACCACTAACCCTAATAACTCCAACATTCATGGACCAGTCGTAGACGAAGTAGAAGGCCTTATTAAAGTTTACAAAGATGGCCACGTGGAACGATCCCAGCTCTTGCCATGTGTTGATCCCTCACTACCCCTCGAGCTCGGCGTGACTTGCTCCGATGTTGTTATAGACAAGTTGACCAACGTATGGGCACGTCTCTATGTCCCGATGACCACGACCAAGTCGTCGGTCTCTAAGCTCCCTTTGATTGTTTACTTCCACGGTGGAGGATTCTGCGTCGGCTCGGCTTCTTGGTTGTGTTACCACGAGTTCTTGGCTAGATTGTCCGCTCGCTCGCGGTGCTTGGTTATGTCTGTAAATTACCGTCTAGCCCCAGAGAACCCTCTTCCAGCAGCTTACGAAGATGGGGTCAATGCCATTCTTTGGCTCAACAAAGCAAGAAACGATAACTTGTGGGCCAAGCAATGTGACTTTGGCAGAATATTCTTGGCAGGAGATAGCGCTGGAGGCAACATCGCGCAGCAAGTCGCGGCAAGATTAGCATCCCCCGAAGATCTAGCACTGAAGATAGAAGGAACCATCCTGATCCAACCTTTCTACAGCGGAGAGGAGCGTACGGAGAGTGAGAGGCGCGTGGGGAACGACAAAACAGCGGTGCTGACACTGGCGTCGTCCGACGCATGGTGGAGGATGTCTTTGCCACGTGGTGCAAACAGAGAGCATCCTTATTGTAAACCTGTGAAGATGATCATCAAGTCGTCGACGGTGACAAGGACGCTGGTGTGCGTGGCCGAGATGGATCTGCTCATGGACAGCAACATGGAGATGTGCGATGGTAATGAGGACGTGATCAAGCGCGTGTTGCACAAAGGCGTTGGTCACGCGTTTCATATTCTCGGAAAGTCTCAGCTTGCTCACACCACAACTCTGGAGATGTTGTGCCAGATCGACGCTTTTATCCACCACTACGATCCCTTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

336

Amino Acids

37.15

Weight (kDa)

6.46

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 76 - 134 5.8e-08 Carboxylesterase family
BD-FAE PF20434 83 - 186 4.4e-12 BD-FAE
Abhydrolase_3 PF07859 92 - 306 1.5e-57 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 250, 694
AccB7I CCANNNNNTGG 2 cut(s) 213, 450
AccBSI CCGCTC 1 cut(s) 356
AccI GTMKAC 2 cut(s) 72, 788
AccII CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
AciI CCGC 5 cut(s) 354, 364, 557, 630, 680
AclWI GGATC 5 cut(s) 117, 140, 607, 834, 992
AcoI YGGCCR 2 cut(s) 109, 816
AcuI CTGAAG 1 cut(s) 611
AcvI CACGTG 2 cut(s) 115, 731
AcyI GRCGYC 1 cut(s) 695
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 1 cut(s) 643
AfeI AGCGCT 1 cut(s) 529
AfiI CCNNNNNNNGG 3 cut(s) 213, 450, 708
AflIII ACRYGT 1 cut(s) 909
AjiI CACGTC 2 cut(s) 221, 874
AluBI AGCT 5 cut(s) 130, 166, 265, 422, 937
AluI AGCT 5 cut(s) 130, 166, 265, 422, 937
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 227, 262, 936
AlwI GGATC 5 cut(s) 117, 140, 607, 834, 992
Ama87I CYCGRG 1 cut(s) 161
Aor51HI AGCGCT 1 cut(s) 529
AoxI GGCC 4 cut(s) 85, 109, 483, 816
ApeKI GCWGC 2 cut(s) 419, 547
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AspLEI GCGC 4 cut(s) 530, 547, 660, 885
AspS9I GGNCC 2 cut(s) 62, 483
AsuHPI GGTGA 1 cut(s) 805
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 111
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 1 cut(s) 332
BbrPI CACGTG 2 cut(s) 115, 731
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 431, 559
BccI CCATC 5 cut(s) 101, 425, 614, 817, 854
BclI TGATCA 2 cut(s) 774, 876
BcoDI GTCTC 3 cut(s) 227, 262, 936
BfaI CTAG 3 cut(s) 345, 395, 584
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 1 cut(s) 531
BglII AGATCT 1 cut(s) 580
BisI GCNGC 3 cut(s) 420, 548, 558
BlsI GCNGC 3 cut(s) 421, 549, 559
Bme18I GGWCC 1 cut(s) 62
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 2 cut(s) 221, 874
BmgT120I GGNCC 2 cut(s) 62, 483
BmiI GGNNCC 1 cut(s) 604
BmsI GCATC 2 cut(s) 578, 757
BpmI CTGGAG 2 cut(s) 552, 977
BsaAI YACGTR 2 cut(s) 115, 731
BsaBI GATNNNNATC 2 cut(s) 568, 776
BsaHI GRCGYC 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 950, 980
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 450, 708
Bse1I ACTGG 2 cut(s) 65, 696
Bse3DI GCAATG 1 cut(s) 497
Bse8I GATNNNNATC 2 cut(s) 568, 776
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 4 cut(s) 569, 606, 723, 748
BseJI GATNNNNATC 2 cut(s) 568, 776
BseLI CCNNNNNNNGG 3 cut(s) 213, 450, 708
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 947
BseNI ACTGG 2 cut(s) 65, 696
BseRI GAGGAG 1 cut(s) 650
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 431, 559
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
BshFI GGCC 4 cut(s) 87, 111, 485, 818
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 161
BsiWI CGTACG 1 cut(s) 641
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 213, 450, 708
BsmAI GTCTC 3 cut(s) 227, 262, 936
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 4 cut(s) 87, 111, 485, 818
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 168, 220
Bsp143I GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
BspACI CCGC 5 cut(s) 354, 364, 557, 630, 680
BspANI GGCC 4 cut(s) 87, 111, 485, 818
BspCNI CTCAG 1 cut(s) 946
BspFNI CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
BspLI GGNNCC 1 cut(s) 604
BspPI GGATC 5 cut(s) 117, 140, 607, 834, 992
BspTNI GGTCTC 1 cut(s) 262
BsrBI CCGCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 497
BsrI ACTGG 2 cut(s) 65, 696
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
BssNI GRCGYC 1 cut(s) 695
BssSI CACGAG 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 332
Bst4CI ACNGT 3 cut(s) 290, 392, 793
Bst6I CTCTTC 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 695
BstBAI YACGTR 2 cut(s) 115, 731
BstC8I GCNNGC 3 cut(s) 358, 362, 939
BstDEI CTNAG 2 cut(s) 261, 933
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 4 cut(s) 569, 606, 723, 748
BstFNI CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 4 cut(s) 530, 547, 660, 885
BstKTI GATC 9 cut(s) 125, 148, 583, 615, 777, 829, 879, 975, 1000
BstMAI GTCTC 3 cut(s) 227, 262, 936
BstMBI GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
BstMWI GCNNNNNNNGC 5 cut(s) 177, 366, 534, 566, 889
BstSFI CTRYAG 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
BstV1I GCAGC 2 cut(s) 431, 559
BstX2I RGATCY 2 cut(s) 580, 826
BstXI CCANNNNNNTGG 1 cut(s) 955
BstYI RGATCY 2 cut(s) 580, 826
BsuRI GGCC 4 cut(s) 87, 111, 485, 818
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 2 cut(s) 526, 873
BtrI CACGTC 2 cut(s) 221, 874
BtsCI GGATG 4 cut(s) 569, 606, 723, 748
BtsIMutI CAGTG 2 cut(s) 587, 689
Cac8I GCNNGC 3 cut(s) 358, 362, 939
CfoI GCGC 4 cut(s) 530, 547, 660, 885
Cfr13I GGNCC 2 cut(s) 62, 483
CseI GACGC 5 cut(s) 292, 684, 713, 811, 986
Csp6I GTAC 1 cut(s) 642
CviAII CATG 6 cut(s) 23, 59, 138, 708, 835, 847
CviQI GTAC 1 cut(s) 642
DdeI CTNAG 2 cut(s) 261, 933
DpnI GATC 9 cut(s) 124, 147, 582, 614, 776, 828, 878, 974, 999
DpnII GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
DraIII CACNNNGTG 1 cut(s) 734
DrdI GACNNNNNNGTC 2 cut(s) 250, 694
DseDI GACNNNNNNGTC 2 cut(s) 250, 694
EaeI YGGCCR 2 cut(s) 109, 816
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 62
Eco47III AGCGCT 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 611
Eco72I CACGTG 2 cut(s) 115, 731
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 168
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 6 cut(s) 26, 62, 141, 711, 838, 850
FaqI GGGAC 1 cut(s) 215
FatI CATG 6 cut(s) 22, 58, 137, 707, 834, 846
FbaI TGATCA 2 cut(s) 774, 876
FblI GTMKAC 2 cut(s) 72, 788
Fnu4HI GCNGC 3 cut(s) 420, 548, 558
FokI GGATG 4 cut(s) 556, 593, 730, 735
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 420, 548, 558
FspBI CTAG 3 cut(s) 345, 395, 584
GlaI GCGC 4 cut(s) 529, 546, 659, 884
GluI GCNGC 3 cut(s) 420, 548, 558
GsaI CCCAGC 1 cut(s) 130
GsuI CTGGAG 2 cut(s) 552, 977
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 4 cut(s) 87, 111, 485, 818
HgaI GACGC 5 cut(s) 292, 684, 713, 811, 986
HhaI GCGC 4 cut(s) 530, 547, 660, 885
Hin1I GRCGYC 1 cut(s) 695
Hin1II CATG 6 cut(s) 26, 62, 141, 711, 838, 850
Hin6I GCGC 4 cut(s) 528, 545, 658, 883
HinP1I GCGC 4 cut(s) 528, 545, 658, 883
HincII GTYRAC 2 cut(s) 204, 789
HindII GTYRAC 2 cut(s) 204, 789
HinfI GANTC 1 cut(s) 297
HphI GGTGA 1 cut(s) 805
Hpy166II GTNNAC 6 cut(s) 73, 100, 204, 280, 761, 789
Hpy188I TCNGA 3 cut(s) 184, 703, 926
Hpy188III TCNNGA 3 cut(s) 232, 610, 956
Hpy8I GTNNAC 6 cut(s) 73, 100, 204, 280, 761, 789
Hpy99I CGWCG 7 cut(s) 256, 308, 700, 707, 790, 793, 980
HpyAV CCTTC 2 cut(s) 77, 593
HpyCH4III ACNGT 3 cut(s) 290, 392, 793
HpyCH4IV ACGT 5 cut(s) 114, 210, 220, 730, 873
HpyCH4V TGCA 2 cut(s) 737, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 366, 534, 566, 889
HpyF3I CTNAG 2 cut(s) 261, 933
HpySE526I ACGT 5 cut(s) 114, 210, 220, 730, 873
Hsp92I GRCGYC 1 cut(s) 695
Hsp92II CATG 6 cut(s) 26, 62, 141, 711, 838, 850
HspAI GCGC 4 cut(s) 528, 545, 658, 883
Ksp22I TGATCA 2 cut(s) 774, 876
Kzo9I GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
LmnI GCTCC 3 cut(s) 185, 270, 637
Lsp1109I GCAGC 2 cut(s) 431, 559
LweI GCATC 2 cut(s) 578, 757
MaeI CTAG 3 cut(s) 345, 395, 584
MaeII ACGT 5 cut(s) 114, 210, 220, 730, 873
MaeIII GTNAC 6 cut(s) 25, 172, 326, 494, 793, 905
MalI GATC 9 cut(s) 124, 147, 582, 614, 776, 828, 878, 974, 999
MbiI CCGCTC 1 cut(s) 356
MboI GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
MboII GAAGA 5 cut(s) 404, 440, 590, 604, 781
MflI RGATCY 2 cut(s) 580, 826
MhlI GDGCHC 2 cut(s) 168, 220
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 3 cut(s) 14, 385, 1006
MluI ACGCGT 1 cut(s) 909
MluNI TGGCCA 1 cut(s) 111
MmeI TCCRAC 3 cut(s) 75, 640, 726
MnlI CCTC 9 cut(s) 160, 170, 287, 420, 527, 628, 648, 708, 862
Mox20I TGGCCA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 111
MseI TTAA 2 cut(s) 93, 1004
MslI CAYNNNNRTG 1 cut(s) 851
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 630, 680
MvnI CGCG 6 cut(s) 364, 545, 557, 660, 885, 911
MwoI GCNNNNNNNGC 5 cut(s) 177, 366, 534, 566, 889
NdeII GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
NlaIII CATG 6 cut(s) 26, 62, 141, 711, 838, 850
NlaIV GGNNCC 1 cut(s) 604
NmeAIII GCCGAG 3 cut(s) 147, 290, 844
NmuCI GTSAC 5 cut(s) 25, 172, 494, 793, 905
PaeR7I CTCGAG 1 cut(s) 161
PceI AGGCCT 1 cut(s) 87
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 248
PfeI GAWTC 1 cut(s) 297
Pfl23II CGTACG 1 cut(s) 641
PflFI GACNNNGTC 1 cut(s) 247
PflMI CCANNNNNTGG 2 cut(s) 213, 450
PkrI GCNGC 3 cut(s) 421, 549, 559
PmaCI CACGTG 2 cut(s) 115, 731
PmlI CACGTG 2 cut(s) 115, 731
Ppu21I YACGTR 2 cut(s) 115, 731
Psp124BI GAGCTC 1 cut(s) 168
PspCI CACGTG 2 cut(s) 115, 731
PspFI CCCAGC 1 cut(s) 126
PspLI CGTACG 1 cut(s) 641
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 2 cut(s) 62, 483
PspXI VCTCGAGB 1 cut(s) 161
PsuI RGATCY 2 cut(s) 580, 826
PsyI GACNNNGTC 1 cut(s) 247
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
RseI CAYNNNNRTG 1 cut(s) 851
SacI GAGCTC 1 cut(s) 168
SalI GTCGAC 1 cut(s) 787
SaqAI TTAA 2 cut(s) 93, 1004
SatI GCNGC 3 cut(s) 420, 548, 558
Sau3AI GATC 9 cut(s) 122, 145, 580, 612, 774, 826, 876, 972, 997
Sau96I GGNCC 2 cut(s) 62, 483
SduI GDGCHC 2 cut(s) 168, 220
SfaNI GCATC 2 cut(s) 578, 757
SfcI CTRYAG 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 161
SgrDI CGTCGACG 1 cut(s) 787
SinI GGWCC 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 851
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 3 cut(s) 14, 385, 1006
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 5 cut(s) 354, 364, 557, 630, 680
SspI AATATT 1 cut(s) 510
SspMI CTAG 3 cut(s) 345, 395, 584
SstI GAGCTC 1 cut(s) 168
StuI AGGCCT 1 cut(s) 87
TaaI ACNGT 3 cut(s) 290, 392, 793
TaiI ACGT 5 cut(s) 117, 213, 223, 733, 876
TaqI TCGA 3 cut(s) 162, 788, 975
TaqII GACCGA 1 cut(s) 244
TasI AATT 3 cut(s) 14, 385, 1006
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 2 cut(s) 93, 1004
Tru9I TTAA 2 cut(s) 93, 1004
TscAI CASTG 2 cut(s) 594, 696
TseFI GTSAC 5 cut(s) 25, 172, 494, 793, 905
TseI GCWGC 2 cut(s) 419, 547
Tsp45I GTSAC 5 cut(s) 25, 172, 494, 793, 905
TspDTI ATGAA 2 cut(s) 47, 905
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 594, 696
Tth111I GACNNNGTC 1 cut(s) 247
Van91I CCANNNNNTGG 2 cut(s) 213, 450
VpaK11BI GGWCC 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 161
XmiI GTMKAC 2 cut(s) 72, 788
XspI CTAG 3 cut(s) 345, 395, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.