MD16G1045600.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014973
Physical Location & Seq
Reverse (-)
3189848 .. 3191026
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1045600.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGAGAGCAAGGAGATCACCAATGGCTGCTATCACCTTAGACACAACTTTCAAGCCAACAACCAACGAAGTACTCGACTCCCCTCATGATCAACAATATGAGACTGTGATCGAGGAAATTGAAGGGCTTATAAAAGTGTACAAGGATGGACACGTGGAGAGACCTCAGATCGTGCCATGTGTCACAGGCGCAGTAGCTCCGGAGCTAGGCGTGACATCGAAGGACGTGGTCATCGACAAGTTCACAAACATCTGGTCACGTTTCTACGTGCCCAAATGCCAAGAAAATAACAAGATGAAGCTCCCTTTGCTAGTATACTTTCATGGAGGTGGGTTTTGTGTTGGCTCAGCCGCTTGGAGTTGTTACCATGATTTTTTAGCAAAGCTAGCCTCCAAACTCGGTTGCTTAATCATGTCGGTAAATTACCGATTGGTCCCTGAACACCCTCTCCCAGCTGCATATGAAGATGGATTCAACTCCATCATGTGGCTGAAACAACAAGCTCTAAGTCAACGTGGAGCTAATAATAATAATACATGGTGGTCAACGCAATGTGACTTTTCCAGAATCTTCCTGGTGGGTGATAGTGCGGGTGCCAATATAGCCCATAATGTGGCCACCAGACTTTACACGCGAGGAGGTTCGGTGTTTGAATCACCTTCTACTGATCAGTATCGCTTGCATCAGTCAAATTCTTACCGTCCATGTGACGAAGAGATTATAAGCTCAATAAAGCCTTTGATTGTTAAAGGTACAATTTTGCTGCAACCCTTTTTTGGGGGAGAGGCTAGGACAAGTTCTGAGAAGTACAATATGGTGAAGCAACCTCGGTCTGTGCTGAGCGTGGCAGCCTCTGATTCTTATTGGCGATTGGCGCTGCCACGTGGCGCTAATCGAGACCATCCATTTTGCAATCCTATGAAGAGTAGTAGTGACGGTGGTACTCTAAAGGCTGGTTGGCCTACTATGGTTTGCATTTCAGAGATGGATATATTGAAAGATAGGAACTTGGAGTTTTGTGAAGCATTGAGGAAAAGAGGAGATGGTAAGATGAAGGTGGAATATGTGGTGTATAAAGGTGTTGGGCATGCATTTCAGGTGCTGGATAAGTCTGAGCTCTCGAAAACTCGATCGCATGAGATGATAAATCATATTAAGGCCTTTGTTAATTGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

393

Amino Acids

43.98

Weight (kDa)

8.65

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 89 - 144 2.5e-07 Carboxylesterase family
BD-FAE PF20434 95 - 207 3.7e-11 BD-FAE
Abhydrolase_3 PF07859 104 - 366 9.3e-58 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 131, 722
AccB1I GGYRCC 1 cut(s) 593
AccB7I CCANNNNNTGG 2 cut(s) 486, 613
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 2 cut(s) 351, 590
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 1 cut(s) 691
AcvI CACGTG 2 cut(s) 154, 884
AfaI GTAC 5 cut(s) 72, 140, 754, 809, 943
AfiI CCNNNNNNNGG 5 cut(s) 206, 486, 613, 776, 777
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 5 cut(s) 52, 122, 475, 653, 997
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 573
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
AluBI AGCT 9 cut(s) 197, 205, 301, 385, 455, 503, 521, 726, 1117
AluI AGCT 9 cut(s) 197, 205, 301, 385, 455, 503, 521, 726, 1117
Alw21I GWGCWC 1 cut(s) 1119
Alw26I GTCTC 3 cut(s) 95, 154, 891
AlwNI CAGNNNCTG 2 cut(s) 854, 1102
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 3 cut(s) 615, 959, 1158
ApeKI GCWGC 5 cut(s) 26, 455, 763, 848, 877
ApoI RAATTY 1 cut(s) 691
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AspLEI GCGC 3 cut(s) 191, 877, 890
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 5 cut(s) 9, 25, 593, 648, 829
AsuNHI GCTAGC 1 cut(s) 385
AvaII GGWCC 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 1119
BbrPI CACGTG 2 cut(s) 154, 884
Bbv12I GWGCWC 1 cut(s) 1119
BbvI GCAGC 5 cut(s) 13, 442, 750, 860, 864
BccI CCATC 6 cut(s) 140, 461, 488, 909, 979, 1037
BciT130I CCWGG 1 cut(s) 575
BclI TGATCA 2 cut(s) 88, 667
BcoDI GTCTC 3 cut(s) 95, 154, 891
BfaI CTAG 4 cut(s) 206, 311, 386, 789
BfoI RGCGCY 2 cut(s) 878, 891
BisI GCNGC 6 cut(s) 27, 351, 456, 764, 849, 878
BlpI GCTNAGC 2 cut(s) 346, 839
BlsI GCNGC 6 cut(s) 28, 352, 457, 765, 850, 879
BmcAI AGTACT 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 433
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 2 cut(s) 435, 595
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 1 cut(s) 691
BmtI GCTAGC 1 cut(s) 389
Bpu1102I GCTNAGC 2 cut(s) 346, 839
BsaAI YACGTR 3 cut(s) 154, 268, 884
BsaBI GATNNNNATC 1 cut(s) 672
BsaI GGTCTC 2 cut(s) 154, 891
BsaJI CCNNGG 1 cut(s) 827
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 630, 660
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 486, 613, 776, 777
Bse3DI GCAATG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 672
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 827
BseGI GGATG 2 cut(s) 151, 901
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 5 cut(s) 206, 486, 613, 776, 777
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 5 cut(s) 179, 360, 792, 830, 1104
BseRI GAGGAG 2 cut(s) 651, 1053
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 5 cut(s) 13, 442, 750, 860, 864
BseYI CCCAGC 1 cut(s) 451
Bsh1236I CGCG 1 cut(s) 634
Bsh1285I CGRYCG 1 cut(s) 1133
BshFI GGCC 3 cut(s) 617, 961, 1160
BshNI GGYRCC 1 cut(s) 593
BsiEI CGRYCG 1 cut(s) 1133
BsiHKAI GWGCWC 1 cut(s) 1119
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 5 cut(s) 206, 486, 613, 776, 777
BsmAI GTCTC 3 cut(s) 95, 154, 891
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 3 cut(s) 617, 961, 1160
Bso31I GGTCTC 2 cut(s) 154, 891
Bsp1286I GDGCHC 2 cut(s) 273, 1119
Bsp13I TCCGGA 1 cut(s) 199
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
Bsp1720I GCTNAGC 2 cut(s) 346, 839
BspACI CCGC 2 cut(s) 351, 590
BspANI GGCC 3 cut(s) 617, 961, 1160
BspCNI CTCAG 5 cut(s) 178, 359, 793, 831, 1105
BspEI TCCGGA 1 cut(s) 199
BspFNI CGCG 1 cut(s) 634
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 2 cut(s) 435, 595
BspOI GCTAGC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 593
BspTNI GGTCTC 2 cut(s) 154, 891
BsrDI GCAATG 1 cut(s) 557
BsrGI TGTACA 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 827
BssMI GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
BssNAI GTATAC 1 cut(s) 316
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 3 cut(s) 106, 701, 938
Bst6I CTCTTC 2 cut(s) 708, 917
BstAUI TGTACA 1 cut(s) 138
BstBAI YACGTR 3 cut(s) 154, 268, 884
BstC8I GCNNGC 3 cut(s) 387, 680, 1089
BstDEI CTNAG 7 cut(s) 37, 165, 346, 506, 801, 839, 1113
BstF5I GGATG 2 cut(s) 151, 901
BstFNI CGCG 1 cut(s) 634
BstH2I RGCGCY 2 cut(s) 878, 891
BstHHI GCGC 3 cut(s) 191, 877, 890
BstKTI GATC 6 cut(s) 17, 91, 111, 171, 670, 1133
BstMAI GTCTC 3 cut(s) 95, 154, 891
BstMBI GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
BstMCI CGRYCG 1 cut(s) 1133
BstMWI GCNNNNNNNGC 4 cut(s) 307, 386, 602, 874
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 1091
BstSCI CCNGG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 5 cut(s) 13, 442, 750, 860, 864
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 617, 961, 1160
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 151, 901
Cac8I GCNNGC 3 cut(s) 387, 680, 1089
CaiI CAGNNNCTG 2 cut(s) 854, 1102
CciI TCATGA 1 cut(s) 85
CfoI GCGC 3 cut(s) 191, 877, 890
Cfr13I GGNCC 1 cut(s) 433
Csp6I GTAC 5 cut(s) 71, 139, 753, 808, 942
CviQI GTAC 5 cut(s) 71, 139, 753, 808, 942
DdeI CTNAG 7 cut(s) 37, 165, 346, 506, 801, 839, 1113
DpnI GATC 6 cut(s) 16, 90, 110, 170, 669, 1132
DpnII GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 708, 917
EarI CTCTTC 2 cut(s) 708, 917
Ecl136II GAGCTC 1 cut(s) 1117
Eco147I AGGCCT 1 cut(s) 1160
Eco24I GRGCYC 1 cut(s) 1119
Eco31I GGTCTC 2 cut(s) 154, 891
Eco47I GGWCC 1 cut(s) 433
Eco53kI GAGCTC 1 cut(s) 1117
Eco72I CACGTG 2 cut(s) 154, 884
EcoICRI GAGCTC 1 cut(s) 1117
EcoRII CCWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 1093
EcoT38I GRGCYC 1 cut(s) 1119
FaqI GGGAC 1 cut(s) 419
FauI CCCGC 1 cut(s) 583
FauNDI CATATG 1 cut(s) 460
FbaI TGATCA 2 cut(s) 88, 667
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 6 cut(s) 27, 351, 456, 764, 849, 878
FokI GGATG 2 cut(s) 158, 888
FriOI GRGCYC 1 cut(s) 1119
Fsp4HI GCNGC 6 cut(s) 27, 351, 456, 764, 849, 878
FspBI CTAG 4 cut(s) 206, 311, 386, 789
GlaI GCGC 3 cut(s) 190, 876, 889
GluI GCNGC 6 cut(s) 27, 351, 456, 764, 849, 878
GsaI CCCAGC 1 cut(s) 455
HaeII RGCGCY 2 cut(s) 878, 891
HaeIII GGCC 3 cut(s) 617, 961, 1160
HapII CCGG 1 cut(s) 200
HhaI GCGC 3 cut(s) 191, 877, 890
Hin6I GCGC 3 cut(s) 189, 875, 888
HinP1I GCGC 3 cut(s) 189, 875, 888
HincII GTYRAC 2 cut(s) 512, 546
HindII GTYRAC 2 cut(s) 512, 546
HinfI GANTC 5 cut(s) 77, 471, 567, 653, 857
HpaII CCGG 1 cut(s) 200
HphI GGTGA 5 cut(s) 9, 25, 593, 648, 829
Hpy166II GTNNAC 5 cut(s) 139, 243, 316, 512, 546
Hpy188I TCNGA 5 cut(s) 168, 802, 856, 982, 1114
Hpy188III TCNNGA 5 cut(s) 86, 200, 564, 896, 1120
Hpy8I GTNNAC 5 cut(s) 139, 243, 316, 512, 546
HpyAV CCTTC 4 cut(s) 116, 214, 669, 1048
HpyCH4III ACNGT 3 cut(s) 106, 701, 938
HpyCH4IV ACGT 6 cut(s) 153, 225, 259, 267, 514, 883
HpyCH4V TGCA 6 cut(s) 458, 682, 766, 912, 975, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 386, 602, 874
HpyF3I CTNAG 7 cut(s) 37, 165, 346, 506, 801, 839, 1113
HpySE526I ACGT 6 cut(s) 153, 225, 259, 267, 514, 883
HspAI GCGC 3 cut(s) 189, 875, 888
Kpn2I TCCGGA 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 88, 667
Kzo9I GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
LmnI GCTCC 4 cut(s) 202, 202, 306, 518
Lsp1109I GCAGC 5 cut(s) 13, 442, 750, 860, 864
LweI GCATC 1 cut(s) 691
MaeI CTAG 4 cut(s) 206, 311, 386, 789
MaeII ACGT 6 cut(s) 153, 225, 259, 267, 514, 883
MaeIII GTNAC 7 cut(s) 181, 211, 255, 362, 554, 707, 932
MalI GATC 6 cut(s) 16, 90, 110, 170, 669, 1132
MboI GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
MboII GAAGA 4 cut(s) 476, 562, 725, 934
MhlI GDGCHC 2 cut(s) 273, 1119
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 6 cut(s) 117, 421, 691, 756, 1168, 1174
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 1093
MroI TCCGGA 1 cut(s) 199
MscI TGGCCA 1 cut(s) 617
MseI TTAA 5 cut(s) 407, 747, 1155, 1167, 1177
MslI CAYNNNNRTG 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 575
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 4 cut(s) 307, 386, 602, 874
NdeI CATATG 1 cut(s) 460
NdeII GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
NheI GCTAGC 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 435, 595
NmuCI GTSAC 6 cut(s) 181, 211, 255, 554, 707, 932
NsiI ATGCAT 1 cut(s) 1093
NspI RCATGY 1 cut(s) 1091
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 1 cut(s) 85
PceI AGGCCT 1 cut(s) 1160
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 231
PfeI GAWTC 4 cut(s) 471, 567, 653, 857
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 2 cut(s) 486, 613
PkrI GCNGC 6 cut(s) 28, 352, 457, 765, 850, 879
Ple19I CGATCG 1 cut(s) 1133
PleI GAGTC 1 cut(s) 71
PmaCI CACGTG 2 cut(s) 154, 884
PmlI CACGTG 2 cut(s) 154, 884
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 3 cut(s) 154, 268, 884
PsiI TTATAA 2 cut(s) 131, 722
Psp124BI GAGCTC 1 cut(s) 1119
Psp6I CCWGG 1 cut(s) 573
PspCI CACGTG 2 cut(s) 154, 884
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 573
PspN4I GGNNCC 2 cut(s) 435, 595
PspPI GGNCC 1 cut(s) 433
PstNI CAGNNNCTG 2 cut(s) 854, 1102
PsyI GACNNNGTC 1 cut(s) 227
PvuI CGATCG 1 cut(s) 1133
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 5 cut(s) 72, 140, 754, 809, 943
RsaNI GTAC 5 cut(s) 71, 139, 753, 808, 942
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 1 cut(s) 1119
SaqAI TTAA 5 cut(s) 407, 747, 1155, 1167, 1177
SatI GCNGC 6 cut(s) 27, 351, 456, 764, 849, 878
Sau3AI GATC 6 cut(s) 14, 88, 108, 168, 667, 1130
Sau96I GGNCC 1 cut(s) 433
ScaI AGTACT 1 cut(s) 72
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 2 cut(s) 273, 1119
SfaNI GCATC 1 cut(s) 691
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 327
SphI GCATGC 1 cut(s) 1091
Sse9I AATT 6 cut(s) 117, 421, 691, 756, 1168, 1174
SseBI AGGCCT 1 cut(s) 1160
SsiI CCGC 2 cut(s) 351, 590
SspMI CTAG 4 cut(s) 206, 311, 386, 789
SstI GAGCTC 1 cut(s) 1119
StuI AGGCCT 1 cut(s) 1160
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 3 cut(s) 106, 701, 938
TaiI ACGT 6 cut(s) 156, 228, 262, 270, 517, 886
TaqI TCGA 7 cut(s) 75, 111, 218, 234, 895, 1121, 1129
TaqII GACCGA 1 cut(s) 819
TasI AATT 6 cut(s) 117, 421, 691, 756, 1168, 1174
TatI WGTACW 3 cut(s) 70, 138, 807
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 4 cut(s) 471, 567, 653, 857
Tru1I TTAA 5 cut(s) 407, 747, 1155, 1167, 1177
Tru9I TTAA 5 cut(s) 407, 747, 1155, 1167, 1177
TseFI GTSAC 6 cut(s) 181, 211, 255, 554, 707, 932
TseI GCWGC 5 cut(s) 26, 455, 763, 848, 877
Tsp45I GTSAC 6 cut(s) 181, 211, 255, 554, 707, 932
TspDTI ATGAA 5 cut(s) 311, 311, 477, 935, 1067
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 2 cut(s) 486, 613
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 4 cut(s) 206, 311, 386, 789
ZrmI AGTACT 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.