Prupe.1G307700_v2.0.a1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014973
Physical Location & Seq
Forward (+)
30015093 .. 30016181
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G307700.1

Sequence Viewer

Length: 1089 bp
ATGGCTGCTATCACCTTAGATCCAAGTTTCAACCCAAAAACCAACCCTCATGAGCATGACCAACAACATGGGAATGTGATTGAGGAAATTGAAGGGCTTATTAAAGTGTACAAAGATGGACATGTGGAGAGACCCCATATTGTTCCATGTGTCGCAACCGCAGTGCCTCCCGAGCTAGGCGTGACATCCAGGGACGTGGTCATTGACAAGTTCACAAACATTTGGTCACGTTTCTATGTTCCCAAATGCCATGGAAATGACAACAAGCTCCCTTTTCTAGTCTATTTCCATGGAGGTGGGTTTTGTGTTGGCTCAGCCGCATGGAGTTGTTACCATGAATTCTTAGCAAAGCTAGCAGCCAAAGCCGGTTGCTTAATCATGTCTGTGAATTATCGACTGGTCCCTGAGCACCCTCTCCCAGCAGCGTATGAAGATGGGTTGAAGGCCCTTTTGTGGCTAAAGCAACAAGCTCTAAGTCAACGCGGGGCTAATAATAAATGGTGGTCAAGGCAGTGTGACTTTTCTAGAATTTTCCTAGTGGGGGACAGTGCGGGTGCCAATATAGCCCATAATGTGGCCACAAGACTTTGCAATATTATTTCTGACGGTCCTTGTGATGAACAAATTATTATTTCAAGCTTAATTAAGCCTTTAACTATCAAGGGTACAATTTTGCTGCAACCATTTTTTGGGGGAGAGGCAAGAACAAGTTCTGAGAAGTACAATATGGTGAAGCAACCTAGGTCTGCTTTGAGCTTGGCAGCTTCTGATACTTACTGGCGATTGGCGCTGCCATGTGGCGCTAATCGCGACCATCCATGGTGCAACCCTTTGAAGAGGGCTAGCTCTCTAAGGGCTGTTTGGGCTACCATGGTTTGCATTTCAGAGATGGATATATTGAAAGATAGAAACTTAGAGTTTTGTTGTGCATTGGATCATAACAGCAACAGAGCTGGTAAGATGAAGGTGGAGTGTGTGGTGTACAAAGGTGTTGGGCATGCATTTCAGGTTCTAGATAAGTCTCAGCTCTCGAAAACTCGATCCCAAGAAATGATAACTCATATTAAGGCCTTTGTTAATTATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

363

Amino Acids

40.38

Weight (kDa)

8.68

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
AccB1I GGYRCC 1 cut(s) 554
AccB7I CCANNNNNTGG 2 cut(s) 574, 689
AccII CGCG 2 cut(s) 483, 810
AciI CCGC 4 cut(s) 159, 318, 483, 551
AclWI GGATC 3 cut(s) 14, 942, 1035
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 2 cut(s) 338, 528
AfaI GTAC 4 cut(s) 110, 667, 722, 983
AfiI CCNNNNNNNGG 5 cut(s) 176, 453, 541, 574, 689
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 6 cut(s) 31, 92, 442, 636, 835, 901
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 188
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 2 cut(s) 124, 1026
AlwI GGATC 3 cut(s) 14, 942, 1035
AlwNI CAGNNNCTG 1 cut(s) 767
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 3 cut(s) 444, 576, 1068
ApeKI GCWGC 6 cut(s) 5, 356, 422, 676, 761, 790
ApoI RAATTY 2 cut(s) 338, 528
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
Asp700I GAANNNNTTC 1 cut(s) 709
AspA2I CCTAGG 1 cut(s) 740
AspLEI GCGC 2 cut(s) 790, 803
AspS9I GGNCC 3 cut(s) 400, 445, 608
AsuHPI GGTGA 2 cut(s) 4, 742
AsuNHI GCTAGC 2 cut(s) 352, 842
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 400, 608
AvrII CCTAGG 1 cut(s) 740
BalI TGGCCA 1 cut(s) 578
BanI GGYRCC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 5 cut(s) 368, 434, 663, 773, 777
BccI CCATC 4 cut(s) 110, 428, 822, 883
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 124, 1026
BfaI CTAG 8 cut(s) 176, 278, 353, 525, 536, 741, 843, 1013
BfoI RGCGCY 2 cut(s) 791, 804
BisI GCNGC 7 cut(s) 6, 318, 357, 423, 677, 762, 791
BlnI CCTAGG 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 313
BlsI GCNGC 7 cut(s) 7, 319, 358, 424, 678, 763, 792
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 2 cut(s) 400, 608
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 400, 445, 608
BmiI GGNNCC 2 cut(s) 402, 556
BmrFI CCNGG 1 cut(s) 190
BmtI GCTAGC 2 cut(s) 356, 846
Bpu10I CCTNAGC 1 cut(s) 405
Bpu1102I GCTNAGC 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 124
BsaJI CCNNGG 6 cut(s) 189, 250, 289, 740, 818, 870
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 453, 541, 574, 689
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 2 cut(s) 402, 782
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 6 cut(s) 189, 250, 289, 740, 818, 870
BseGI GGATG 2 cut(s) 185, 814
BseLI CCNNNNNNNGG 5 cut(s) 176, 453, 541, 574, 689
BseMII CTCAG 4 cut(s) 327, 396, 705, 1037
BseNI ACTGG 2 cut(s) 402, 782
BseXI GCAGC 5 cut(s) 368, 434, 663, 773, 777
BseYI CCCAGC 1 cut(s) 418
Bsh1236I CGCG 2 cut(s) 483, 810
BshFI GGCC 3 cut(s) 446, 578, 1070
BshNI GGYRCC 1 cut(s) 554
BsiHKAI GWGCWC 1 cut(s) 411
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 366
BslFI GGGAC 3 cut(s) 206, 386, 557
BslI CCNNNNNNNGG 5 cut(s) 176, 453, 541, 574, 689
BsmAI GTCTC 2 cut(s) 124, 1026
BsmFI GGGAC 3 cut(s) 206, 386, 557
BsnI GGCC 3 cut(s) 446, 578, 1070
Bso31I GGTCTC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 411
Bsp1407I TGTACA 2 cut(s) 108, 981
Bsp143I GATC 3 cut(s) 19, 934, 1040
Bsp1720I GCTNAGC 1 cut(s) 313
Bsp19I CCATGG 4 cut(s) 250, 289, 818, 870
Bsp68I TCGCGA 1 cut(s) 810
BspACI CCGC 4 cut(s) 159, 318, 483, 551
BspANI GGCC 3 cut(s) 446, 578, 1070
BspCNI CTCAG 4 cut(s) 326, 397, 706, 1036
BspFNI CGCG 2 cut(s) 483, 810
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 402, 556
BspOI GCTAGC 2 cut(s) 356, 846
BspPI GGATC 3 cut(s) 14, 942, 1035
BspT107I GGYRCC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 365
BsrGI TGTACA 2 cut(s) 108, 981
BsrI ACTGG 2 cut(s) 402, 782
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 6 cut(s) 189, 250, 289, 740, 818, 870
BssMI GATC 3 cut(s) 19, 934, 1040
BssT1I CCWWGG 5 cut(s) 250, 289, 740, 818, 870
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 2 cut(s) 548, 608
Bst6I CTCTTC 1 cut(s) 830
BstAUI TGTACA 2 cut(s) 108, 981
BstC8I GCNNGC 3 cut(s) 354, 844, 999
BstDEI CTNAG 9 cut(s) 16, 313, 343, 405, 473, 714, 851, 913, 1023
BstDSI CCRYGG 4 cut(s) 250, 289, 818, 870
BstF5I GGATG 2 cut(s) 185, 814
BstFNI CGCG 2 cut(s) 483, 810
BstH2I RGCGCY 2 cut(s) 791, 804
BstHHI GCGC 2 cut(s) 790, 803
BstKTI GATC 3 cut(s) 22, 937, 1043
BstMAI GTCTC 2 cut(s) 124, 1026
BstMBI GATC 3 cut(s) 19, 934, 1040
BstMWI GCNNNNNNNGC 7 cut(s) 172, 353, 362, 563, 787, 807, 863
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 2 cut(s) 125, 1001
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 2 cut(s) 483, 810
BstV1I GCAGC 5 cut(s) 368, 434, 663, 773, 777
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 3 cut(s) 68, 196, 296
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 3 cut(s) 446, 578, 1070
BtgI CCRYGG 4 cut(s) 250, 289, 818, 870
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 2 cut(s) 185, 814
BtsI GCAGTG 2 cut(s) 168, 518
BtsIMutI CAGTG 3 cut(s) 168, 518, 553
BtuMI TCGCGA 1 cut(s) 810
Cac8I GCNNGC 3 cut(s) 354, 844, 999
CaiI CAGNNNCTG 1 cut(s) 767
CciI TCATGA 1 cut(s) 49
CfoI GCGC 2 cut(s) 790, 803
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 3 cut(s) 400, 445, 608
Csp6I GTAC 4 cut(s) 109, 666, 721, 982
CviQI GTAC 4 cut(s) 109, 666, 721, 982
DdeI CTNAG 9 cut(s) 16, 313, 343, 405, 473, 714, 851, 913, 1023
DpnI GATC 3 cut(s) 21, 936, 1042
DpnII GATC 3 cut(s) 19, 934, 1040
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 830
EarI CTCTTC 1 cut(s) 830
Eco130I CCWWGG 5 cut(s) 250, 289, 740, 818, 870
Eco147I AGGCCT 1 cut(s) 1070
Eco31I GGTCTC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 400, 608
Eco88I CYCGRG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 5 cut(s) 250, 289, 740, 818, 870
EcoT22I ATGCAT 1 cut(s) 1003
ErhI CCWWGG 5 cut(s) 250, 289, 740, 818, 870
FaqI GGGAC 3 cut(s) 206, 386, 557
FauI CCCGC 2 cut(s) 476, 544
Fnu4HI GCNGC 7 cut(s) 6, 318, 357, 423, 677, 762, 791
FokI GGATG 2 cut(s) 172, 801
Fsp4HI GCNGC 7 cut(s) 6, 318, 357, 423, 677, 762, 791
FspBI CTAG 8 cut(s) 176, 278, 353, 525, 536, 741, 843, 1013
GlaI GCGC 2 cut(s) 789, 802
GluI GCNGC 7 cut(s) 6, 318, 357, 423, 677, 762, 791
GsaI CCCAGC 1 cut(s) 422
HaeII RGCGCY 2 cut(s) 791, 804
HaeIII GGCC 3 cut(s) 446, 578, 1070
HapII CCGG 1 cut(s) 366
HhaI GCGC 2 cut(s) 790, 803
Hin6I GCGC 2 cut(s) 788, 801
HinP1I GCGC 2 cut(s) 788, 801
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 637
HpaII CCGG 1 cut(s) 366
HphI GGTGA 2 cut(s) 4, 742
Hpy166II GTNNAC 4 cut(s) 109, 213, 479, 982
Hpy188I TCNGA 4 cut(s) 604, 715, 769, 886
Hpy188III TCNNGA 6 cut(s) 50, 170, 525, 809, 1013, 1030
Hpy8I GTNNAC 4 cut(s) 109, 213, 479, 982
HpyAV CCTTC 3 cut(s) 86, 436, 958
HpyCH4III ACNGT 2 cut(s) 548, 608
HpyCH4IV ACGT 2 cut(s) 195, 229
HpyCH4V TGCA 6 cut(s) 591, 679, 825, 879, 929, 1001
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 353, 362, 563, 787, 807, 863
HpyF3I CTNAG 9 cut(s) 16, 313, 343, 405, 473, 714, 851, 913, 1023
HpySE526I ACGT 2 cut(s) 195, 229
HspAI GCGC 2 cut(s) 788, 801
Kzo9I GATC 3 cut(s) 19, 934, 1040
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 9 cut(s) 175, 202, 379, 383, 417, 432, 763, 939, 992
Lsp1109I GCAGC 5 cut(s) 368, 434, 663, 773, 777
MaeI CTAG 8 cut(s) 176, 278, 353, 525, 536, 741, 843, 1013
MaeII ACGT 2 cut(s) 195, 229
MaeIII GTNAC 4 cut(s) 181, 225, 329, 515
MalI GATC 3 cut(s) 21, 936, 1042
MboI GATC 3 cut(s) 19, 934, 1040
MboII GAAGA 2 cut(s) 443, 847
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 9 cut(s) 87, 338, 388, 528, 624, 642, 669, 1078, 1084
MluNI TGGCCA 1 cut(s) 578
MnlI CCTC 7 cut(s) 57, 76, 177, 287, 423, 691, 831
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 1003
MroXI GAANNNNTTC 1 cut(s) 709
MscI TGGCCA 1 cut(s) 578
MseI TTAA 7 cut(s) 102, 374, 641, 645, 653, 1065, 1077
MslI CAYNNNNRTG 5 cut(s) 54, 72, 255, 294, 383
Msp20I TGGCCA 1 cut(s) 578
MspI CCGG 1 cut(s) 366
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 2 cut(s) 483, 810
MwoI GCNNNNNNNGC 7 cut(s) 172, 353, 362, 563, 787, 807, 863
NcoI CCATGG 4 cut(s) 250, 289, 818, 870
NdeII GATC 3 cut(s) 19, 934, 1040
NheI GCTAGC 2 cut(s) 352, 842
NlaIV GGNNCC 2 cut(s) 402, 556
NmuCI GTSAC 3 cut(s) 181, 225, 515
NruI TCGCGA 1 cut(s) 810
NsiI ATGCAT 1 cut(s) 1003
NspI RCATGY 2 cut(s) 125, 1001
PacI TTAATTAA 1 cut(s) 645
PaeI GCATGC 1 cut(s) 1001
PagI TCATGA 1 cut(s) 49
PceI AGGCCT 1 cut(s) 1070
PciI ACATGT 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 709
PflFI GACNNNGTC 1 cut(s) 197
PflMI CCANNNNNTGG 2 cut(s) 574, 689
PkrI GCNGC 7 cut(s) 7, 319, 358, 424, 678, 763, 792
PscI ACATGT 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1083
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 402, 556
PspPI GGNCC 3 cut(s) 400, 445, 608
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 197
RruI TCGCGA 1 cut(s) 810
RsaI GTAC 4 cut(s) 110, 667, 722, 983
RsaNI GTAC 4 cut(s) 109, 666, 721, 982
RseI CAYNNNNRTG 5 cut(s) 54, 72, 255, 294, 383
SaqAI TTAA 7 cut(s) 102, 374, 641, 645, 653, 1065, 1077
SatI GCNGC 7 cut(s) 6, 318, 357, 423, 677, 762, 791
Sau3AI GATC 3 cut(s) 19, 934, 1040
Sau96I GGNCC 3 cut(s) 400, 445, 608
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 411
SinI GGWCC 2 cut(s) 400, 608
SmiMI CAYNNNNRTG 5 cut(s) 54, 72, 255, 294, 383
SphI GCATGC 1 cut(s) 1001
Sse9I AATT 9 cut(s) 87, 338, 388, 528, 624, 642, 669, 1078, 1084
SseBI AGGCCT 1 cut(s) 1070
SsiI CCGC 4 cut(s) 159, 318, 483, 551
SspI AATATT 1 cut(s) 595
SspMI CTAG 8 cut(s) 176, 278, 353, 525, 536, 741, 843, 1013
StuI AGGCCT 1 cut(s) 1070
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 5 cut(s) 250, 289, 740, 818, 870
TaaI ACNGT 2 cut(s) 548, 608
TaiI ACGT 2 cut(s) 198, 232
TaqI TCGA 3 cut(s) 394, 1031, 1039
TasI AATT 9 cut(s) 87, 338, 388, 528, 624, 642, 669, 1078, 1084
TatI WGTACW 3 cut(s) 108, 720, 981
TauI GCSGC 1 cut(s) 320
Tru1I TTAA 7 cut(s) 102, 374, 641, 645, 653, 1065, 1077
Tru9I TTAA 7 cut(s) 102, 374, 641, 645, 653, 1065, 1077
TscAI CASTG 3 cut(s) 168, 518, 553
TseFI GTSAC 3 cut(s) 181, 225, 515
TseI GCWGC 6 cut(s) 5, 356, 422, 676, 761, 790
Tsp45I GTSAC 3 cut(s) 181, 225, 515
TspDTI ATGAA 4 cut(s) 351, 444, 633, 977
TspRI CASTG 3 cut(s) 168, 518, 553
Tth111I GACNNNGTC 1 cut(s) 197
Van91I CCANNNNNTGG 2 cut(s) 574, 689
VpaK11BI GGWCC 2 cut(s) 400, 608
XapI RAATTY 2 cut(s) 338, 528
XbaI TCTAGA 2 cut(s) 524, 1012
XceI RCATGY 2 cut(s) 125, 1001
XmaJI CCTAGG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 709
XspI CTAG 8 cut(s) 176, 278, 353, 525, 536, 741, 843, 1013
Zsp2I ATGCAT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.