FvH4_4g27710

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014973
Physical Location & Seq
Reverse (-)
28583992 .. 28585080
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27710.t1

Sequence Viewer

Length: 1089 bp
ATGGTGCCTGCTATCACCTTGGATCCAAGGCTTCCAAGCTATCTGAAGCAAAAGACCAACAACACAGTCGACCATCATCAGCAGCAAGAGAACTTTGTGATCGAAGAAATCGAAGGGCTTATCAGAGTATACAAAGACGGACGTGTCGAGAGACCTCAGATCGTGCCGTGCGTCACGGCCGGCGCCGCCGATCCGGAGTTAGGCGTGACATCCATGGACATAGTCATTGACAGGTTCACAAACGTTTCCTCACGTTTCTATGTTCCGATACGCCGCGACATGAATAATCCGATGAAGAAGCTCCCTCTGCTAGTGTATTTTCACGGAGGTGGGTTTTGCGTTGGCTCCGCCGCCTGGAGTTGCTACCATGAATTTTTGGCAAAGCTAGCAGCCAAGGCCGGTTGCATAATCATGTCGGTAAATTACCGTTTGGCTCCTGAACACCCTCTTCCGGCAGCGTATGAAGATGGACTCAGATCCCTTGTGTGGCTAAAGCAAGAAGCTTCTTGTGAACGTGGTGGGGAGGAGGAGAGTAACCAGTGGTGGTCGACGCAGTGCGACTTTTCTAGAATATTCTTGGTGGGGGATAGTGCTGGTGCCAACATAGCCCATAATGTGAGCAGGAAACTGCTTTGTTCTGAGACATTTCTATTGCCTTTAATTATTAAAGGTGTAATTCTGGTGCAACCATTTTTTGGGGGAGAAGACAGGACGAGTTCGGAGAAGAACCACGACATGGTGCAGCCACGGCGTCGCTCGGTGTTGAACCTGGCAGCTTCGGATACCTACTGGAGATTGGCACTGCCAGCCGGTTCGAACCGCGACCATCCATGGTGCAACCCTTTGGCTAACGGCTCCTGTTTTGGCTCGGATAGCGTGGCGATGATGGTGTGTATTTCGGAGCTGGACATTTTGAAAGATAGGGAGTTCGAGTTTTGTGGTGCAATGGTTAGAGCAGGGAAAAAGGTTGAGACTGTGGTGTACAGAGGGGTAGGGCATGCGTTTCAGGTACTAGATAAGTCTATGCTTGCACAAACTCGATGCCAGGAGATGATAACTCATATTAAGGCCTTTGTTGAAAACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

363

Amino Acids

40.53

Weight (kDa)

6.56

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 92 - 145 4.3e-07 Carboxylesterase family
BD-FAE PF20434 98 - 204 1.9e-10 BD-FAE
Abhydrolase_3 PF07859 104 - 336 1.2e-59 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AccB1I GGYRCC 3 cut(s) 4, 182, 596
AccB7I CCANNNNNTGG 2 cut(s) 695, 736
AccI GTMKAC 3 cut(s) 69, 129, 548
AccII CGCG 2 cut(s) 276, 822
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 5 cut(s) 186, 274, 348, 351, 820
AclI AACGTT 1 cut(s) 243
AclWI GGATC 4 cut(s) 17, 30, 185, 471
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 2 cut(s) 183, 751
AfaI GTAC 2 cut(s) 983, 1011
AfiI CCNNNNNNNGG 6 cut(s) 200, 354, 451, 486, 695, 736
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 3 cut(s) 766, 916, 1079
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 3 cut(s) 353, 768, 1044
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 6 cut(s) 39, 301, 385, 503, 776, 904
AluI AGCT 6 cut(s) 39, 301, 385, 503, 776, 904
Alw26I GTCTC 3 cut(s) 145, 635, 965
AlwI GGATC 4 cut(s) 17, 30, 185, 471
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 3 cut(s) 177, 396, 1068
ApeKI GCWGC 5 cut(s) 82, 389, 455, 742, 773
ApoI RAATTY 1 cut(s) 371
AspLEI GCGC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 7
AsuII TTCGAA 1 cut(s) 815
AsuNHI GCTAGC 1 cut(s) 385
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 3 cut(s) 4, 182, 596
BbsI GAAGAC 1 cut(s) 711
BbvI GCAGC 5 cut(s) 94, 401, 467, 754, 785
BccI CCATC 4 cut(s) 81, 461, 834, 880
BceAI ACGGC 4 cut(s) 151, 192, 764, 868
BciT130I CCWGG 3 cut(s) 355, 770, 1046
BciVI GTATCC 1 cut(s) 775
BcoDI GTCTC 3 cut(s) 145, 635, 965
BfaI CTAG 5 cut(s) 311, 386, 567, 1013, 1087
BfoI RGCGCY 1 cut(s) 186
BfuI GTATCC 1 cut(s) 775
BisI GCNGC 8 cut(s) 83, 186, 274, 351, 390, 456, 743, 774
BlsI GCNGC 8 cut(s) 84, 187, 275, 352, 391, 457, 744, 775
Bme1390I CCNGG 3 cut(s) 355, 770, 1046
BmgBI CACGTC 1 cut(s) 143
BmiI GGNNCC 7 cut(s) 6, 24, 184, 346, 435, 598, 856
BmrFI CCNGG 3 cut(s) 355, 770, 1046
BmsI GCATC 1 cut(s) 1031
BmtI GCTAGC 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 376, 811
Bpu14I TTCGAA 1 cut(s) 815
BsaHI GRCGYC 2 cut(s) 183, 751
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 6 cut(s) 18, 26, 213, 393, 746, 830
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 6 cut(s) 200, 354, 451, 486, 695, 736
Bse118I RCCGGY 3 cut(s) 179, 398, 809
Bse1I ACTGG 2 cut(s) 538, 794
Bse3DI GCAATG 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 3 cut(s) 355, 770, 1046
BseDI CCNNGG 6 cut(s) 18, 26, 213, 393, 746, 830
BseGI GGATG 2 cut(s) 209, 826
BseLI CCNNNNNNNGG 6 cut(s) 200, 354, 451, 486, 695, 736
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 3 cut(s) 170, 487, 630
BseNI ACTGG 2 cut(s) 538, 794
BseRI GAGGAG 2 cut(s) 539, 542
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 5 cut(s) 94, 401, 467, 754, 785
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 2 cut(s) 276, 822
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 3 cut(s) 179, 398, 1070
BshNI GGYRCC 3 cut(s) 4, 182, 596
BsiEI CGRYCG 1 cut(s) 180
BsiSI CCGG 5 cut(s) 180, 194, 399, 452, 810
BslI CCNNNNNNNGG 6 cut(s) 200, 354, 451, 486, 695, 736
BsmAI GTCTC 3 cut(s) 145, 635, 965
BsnI GGCC 3 cut(s) 179, 398, 1070
Bso31I GGTCTC 1 cut(s) 145
Bsp119I TTCGAA 1 cut(s) 815
Bsp13I TCCGGA 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 5 cut(s) 22, 99, 159, 190, 476
Bsp19I CCATGG 2 cut(s) 213, 830
BspACI CCGC 5 cut(s) 186, 274, 348, 351, 820
BspANI GGCC 3 cut(s) 179, 398, 1070
BspCNI CTCAG 3 cut(s) 169, 486, 631
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 2 cut(s) 276, 822
BspLI GGNNCC 7 cut(s) 6, 24, 184, 346, 435, 598, 856
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 4 cut(s) 17, 30, 185, 471
BspT104I TTCGAA 1 cut(s) 815
BspT107I GGYRCC 3 cut(s) 4, 182, 596
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 3 cut(s) 179, 398, 809
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 2 cut(s) 538, 794
BssAI RCCGGY 3 cut(s) 179, 398, 809
BssECI CCNNGG 6 cut(s) 18, 26, 213, 393, 746, 830
BssMI GATC 5 cut(s) 22, 99, 159, 190, 476
BssNAI GTATAC 1 cut(s) 130
BssNI GRCGYC 2 cut(s) 183, 751
BssT1I CCWWGG 5 cut(s) 18, 26, 213, 393, 830
Bst1107I GTATAC 1 cut(s) 130
Bst2UI CCWGG 3 cut(s) 355, 770, 1046
Bst4CI ACNGT 3 cut(s) 67, 428, 976
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 2 cut(s) 183, 751
BstAUI TGTACA 1 cut(s) 981
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 6 cut(s) 9, 181, 387, 807, 999, 1029
BstDEI CTNAG 3 cut(s) 156, 473, 639
BstDSI CCRYGG 3 cut(s) 213, 746, 830
BstF5I GGATG 2 cut(s) 209, 826
BstFNI CGCG 2 cut(s) 276, 822
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 5 cut(s) 25, 102, 162, 193, 479
BstMAI GTCTC 3 cut(s) 145, 635, 965
BstMBI GATC 5 cut(s) 22, 99, 159, 190, 476
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 8 cut(s) 185, 307, 386, 395, 605, 748, 806, 873
BstNI CCWGG 3 cut(s) 355, 770, 1046
BstNSI RCATGY 1 cut(s) 1001
BstSCI CCNGG 3 cut(s) 353, 768, 1044
BstUI CGCG 2 cut(s) 276, 822
BstV1I GCAGC 5 cut(s) 94, 401, 467, 754, 785
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 2 cut(s) 22, 476
BstYI RGATCY 2 cut(s) 22, 476
BstZ17I GTATAC 1 cut(s) 130
BstZI CGGCCG 1 cut(s) 177
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 3 cut(s) 179, 398, 1070
BtgI CCRYGG 3 cut(s) 213, 746, 830
BtgZI GCGATG 1 cut(s) 896
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 2 cut(s) 209, 826
BtsI GCAGTG 2 cut(s) 560, 800
BtsIMutI CAGTG 3 cut(s) 545, 560, 800
Cac8I GCNNGC 6 cut(s) 9, 181, 387, 807, 999, 1029
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 3 cut(s) 179, 398, 809
CseI GACGC 3 cut(s) 160, 559, 740
Csp6I GTAC 2 cut(s) 982, 1010
CviAII CATG 7 cut(s) 214, 280, 368, 412, 736, 831, 998
CviQI GTAC 2 cut(s) 982, 1010
DdeI CTNAG 3 cut(s) 156, 473, 639
DinI GGCGCC 1 cut(s) 184
DpnI GATC 5 cut(s) 24, 101, 161, 192, 478
DpnII GATC 5 cut(s) 22, 99, 159, 190, 476
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 177
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 1 cut(s) 337
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 5 cut(s) 18, 26, 213, 393, 830
Eco147I AGGCCT 1 cut(s) 1070
Eco31I GGTCTC 1 cut(s) 145
Eco52I CGGCCG 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 65
EcoRII CCWGG 3 cut(s) 353, 768, 1044
EcoT14I CCWWGG 5 cut(s) 18, 26, 213, 393, 830
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 5 cut(s) 18, 26, 213, 393, 830
FaeI CATG 7 cut(s) 217, 283, 371, 415, 739, 834, 1001
FatI CATG 7 cut(s) 213, 279, 367, 411, 735, 830, 997
FblI GTMKAC 3 cut(s) 69, 129, 548
Fnu4HI GCNGC 8 cut(s) 83, 186, 274, 351, 390, 456, 743, 774
FokI GGATG 2 cut(s) 196, 813
Fsp4HI GCNGC 8 cut(s) 83, 186, 274, 351, 390, 456, 743, 774
FspBI CTAG 5 cut(s) 311, 386, 567, 1013, 1087
GlaI GCGC 1 cut(s) 184
GluI GCNGC 8 cut(s) 83, 186, 274, 351, 390, 456, 743, 774
GsuI CTGGAG 2 cut(s) 376, 811
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 3 cut(s) 179, 398, 1070
HapII CCGG 5 cut(s) 180, 194, 399, 452, 810
HgaI GACGC 3 cut(s) 160, 559, 740
HhaI GCGC 1 cut(s) 185
Hin1I GRCGYC 2 cut(s) 183, 751
Hin1II CATG 7 cut(s) 217, 283, 371, 415, 739, 834, 1001
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 2 cut(s) 70, 549
HindII GTYRAC 2 cut(s) 70, 549
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 1 cut(s) 471
HpaII CCGG 5 cut(s) 180, 194, 399, 452, 810
HphI GGTGA 1 cut(s) 7
Hpy166II GTNNAC 6 cut(s) 70, 130, 237, 512, 549, 982
Hpy188III TCNNGA 4 cut(s) 148, 194, 437, 567
Hpy8I GTNNAC 6 cut(s) 70, 130, 237, 512, 549, 982
Hpy99I CGWCG 2 cut(s) 553, 756
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 3 cut(s) 67, 428, 976
HpyCH4IV ACGT 4 cut(s) 142, 243, 253, 514
HpyCH4V TGCA 6 cut(s) 405, 685, 742, 837, 944, 1031
HpyF10VI GCNNNNNNNGC 8 cut(s) 185, 307, 386, 395, 605, 748, 806, 873
HpyF3I CTNAG 3 cut(s) 156, 473, 639
HpySE526I ACGT 4 cut(s) 142, 243, 253, 514
Hsp92I GRCGYC 2 cut(s) 183, 751
Hsp92II CATG 7 cut(s) 217, 283, 371, 415, 739, 834, 1001
HspAI GCGC 1 cut(s) 183
KasI GGCGCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 193
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 5 cut(s) 22, 99, 159, 190, 476
LmnI GCTCC 5 cut(s) 306, 350, 439, 860, 901
Lsp1109I GCAGC 5 cut(s) 94, 401, 467, 754, 785
LweI GCATC 1 cut(s) 1031
MaeI CTAG 5 cut(s) 311, 386, 567, 1013, 1087
MaeII ACGT 4 cut(s) 142, 243, 253, 514
MaeIII GTNAC 3 cut(s) 172, 205, 533
MalI GATC 5 cut(s) 24, 101, 161, 192, 478
MboI GATC 5 cut(s) 22, 99, 159, 190, 476
MboII GAAGA 6 cut(s) 116, 307, 440, 476, 716, 736
MflI RGATCY 2 cut(s) 22, 476
MluCI AATT 4 cut(s) 371, 421, 660, 675
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 1 cut(s) 465
MnlI CCTC 8 cut(s) 165, 259, 315, 320, 456, 517, 520, 980
MroI TCCGGA 1 cut(s) 193
MroNI GCCGGC 1 cut(s) 179
MseI TTAA 3 cut(s) 659, 666, 1065
MslI CAYNNNNRTG 2 cut(s) 327, 410
MspI CCGG 5 cut(s) 180, 194, 399, 452, 810
MspR9I CCNGG 3 cut(s) 355, 770, 1046
MvaI CCWGG 3 cut(s) 355, 770, 1046
MvnI CGCG 2 cut(s) 276, 822
MwoI GCNNNNNNNGC 8 cut(s) 185, 307, 386, 395, 605, 748, 806, 873
NaeI GCCGGC 1 cut(s) 181
NarI GGCGCC 1 cut(s) 183
NcoI CCATGG 2 cut(s) 213, 830
NdeII GATC 5 cut(s) 22, 99, 159, 190, 476
NgoMIV GCCGGC 1 cut(s) 179
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 7 cut(s) 217, 283, 371, 415, 739, 834, 1001
NlaIV GGNNCC 7 cut(s) 6, 24, 184, 346, 435, 598, 856
NmuCI GTSAC 2 cut(s) 172, 205
NspI RCATGY 1 cut(s) 1001
NspV TTCGAA 1 cut(s) 815
OliI CACNNNNGTG 1 cut(s) 327
PaeI GCATGC 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 3 cut(s) 108, 144, 168
PdiI GCCGGC 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 2 cut(s) 695, 736
PkrI GCNGC 8 cut(s) 84, 187, 275, 352, 391, 457, 744, 775
PleI GAGTC 1 cut(s) 465
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 243
Psp6I CCWGG 3 cut(s) 353, 768, 1044
PspGI CCWGG 3 cut(s) 353, 768, 1044
PspN4I GGNNCC 7 cut(s) 6, 24, 184, 346, 435, 598, 856
PsuI RGATCY 2 cut(s) 22, 476
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 2 cut(s) 983, 1011
RsaNI GTAC 2 cut(s) 982, 1010
RseI CAYNNNNRTG 2 cut(s) 327, 410
SalI GTCGAC 2 cut(s) 68, 547
SaqAI TTAA 3 cut(s) 659, 666, 1065
SatI GCNGC 8 cut(s) 83, 186, 274, 351, 390, 456, 743, 774
Sau3AI GATC 5 cut(s) 22, 99, 159, 190, 476
SchI GAGTC 1 cut(s) 465
ScrFI CCNGG 3 cut(s) 355, 770, 1046
SfaNI GCATC 1 cut(s) 1031
SfoI GGCGCC 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 327, 410
SphI GCATGC 1 cut(s) 1001
Sse9I AATT 4 cut(s) 371, 421, 660, 675
SseBI AGGCCT 1 cut(s) 1070
SsiI CCGC 5 cut(s) 186, 274, 348, 351, 820
SspDI GGCGCC 1 cut(s) 182
SspI AATATT 1 cut(s) 573
SspMI CTAG 5 cut(s) 311, 386, 567, 1013, 1087
StuI AGGCCT 1 cut(s) 1070
StyD4I CCNGG 3 cut(s) 353, 768, 1044
StyI CCWWGG 5 cut(s) 18, 26, 213, 393, 830
TaaI ACNGT 3 cut(s) 67, 428, 976
TaiI ACGT 4 cut(s) 145, 246, 256, 517
TaqI TCGA 8 cut(s) 69, 102, 111, 147, 548, 815, 930, 1039
TasI AATT 4 cut(s) 371, 421, 660, 675
TatI WGTACW 1 cut(s) 981
TauI GCSGC 3 cut(s) 188, 276, 353
Tru1I TTAA 3 cut(s) 659, 666, 1065
Tru9I TTAA 3 cut(s) 659, 666, 1065
TscAI CASTG 3 cut(s) 545, 560, 807
TseFI GTSAC 2 cut(s) 172, 205
TseI GCWGC 5 cut(s) 82, 389, 455, 742, 773
Tsp45I GTSAC 2 cut(s) 172, 205
TspDTI ATGAA 4 cut(s) 296, 308, 384, 477
TspGWI ACGGA 2 cut(s) 153, 339
TspRI CASTG 3 cut(s) 545, 560, 807
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 2 cut(s) 695, 736
XapI RAATTY 1 cut(s) 371
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 1 cut(s) 1001
XmiI GTMKAC 3 cut(s) 69, 129, 548
XspI CTAG 5 cut(s) 311, 386, 567, 1013, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.