Rh4AG342100

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014973
Physical Location & Seq
Reverse (-)
65069260 .. 65070330
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG342100.1

Sequence Viewer

Length: 1071 bp
ATGGTGCCTGCTATCACCTTAGATCCAAGCTATTTCAAGCAAAAGACCAACAACACAGTCGACCATCATCACCAGCATGAGAACATTGTGATCGAGGAAATCAAAGGGCTTATCAAAGTGTACAAAGACGGACATGTGGAGAGACCGCAGATCGTCCCGTGCGTCACTGCCGCTGTGGCCGATCCGGAGTTAGGCGTGACATCCATGGACATAGTCATTGACAGGTTCACAAACGTTTCCTCACGTTTCTATGTTCCGAAACGCCACGGGATGAATTCGATGAAGAAGCTTCCTTTGCTGGTGTATTTTCACGGAGGTGGGTTTTGCGCTGGCTCCACCGCTTGGAGTTGTTACCATGAATTTTTGGCAAAGCTGGCGGCCAAAGCTGGTTGCGTAATCATGTCGGTAAATTACCGGTTGGCGCCCGAACACCCTCTTCCGGCGGCTTATGAAGATGGACTCAGAGCCCTTGTGTGGCTAAAACAACAAGCTTTTTGTGAACGTGGTGGGGAGGAGAATAACCAGTGGTGGTCAACGCAGTGCGACTTTTCTAGAATATTCTTGGTGGGGGATAGTGCTGGTGCCAATATAGCCCATAATGTGATCACAAGACTGCTTTGTTCTGGGACAATTCTATTGCCTTTAATAATTAAAGGTGTAATTCTGGTGCAACCATTTTTTGGGGGAGAAGCAAGGACGAGTTCGGAGAAGTACTACTACATGGTGCAGCCACGTCACTCGGTGTTGAATCTGGCAGCTTCGGATACCTACTGGAGATTGGCACTGCCGGCCGGTTCCAACCGCGACCATCCATGGTGCAACCCTTTGGCTAACGGCTCGTGCTTCGGTTCGGATAGCGTGGCTATGATGGTGTGTATTTCGGAGTTGGACATATTGAAAGATAGGGAGTTAGAGTTTTGTGCTGCAATGGCTAGAGCAGGCAAGAACGTAGAGACTGTGGTGTACAGAGGTGTAGGGCACGCGTTTCAGGTACTAGATAAGTCTATGCTCGCACAAACTCGATGCCATGAGATGATAGCTCATATTAAGGCCTTTGTTGAAAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

356

Amino Acids

39.63

Weight (kDa)

7.22

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 84 - 141 4.5e-07 Carboxylesterase family
BD-FAE PF20434 92 - 198 7.5e-12 BD-FAE
Abhydrolase_3 PF07859 100 - 330 3.3e-61 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 4, 421, 581
AccB7I CCANNNNNTGG 2 cut(s) 342, 680
AccI GTMKAC 1 cut(s) 60
AccII CGCG 2 cut(s) 804, 983
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 6 cut(s) 146, 171, 339, 377, 443, 802
AclI AACGTT 1 cut(s) 234
AclWI GGATC 2 cut(s) 17, 176
AcoI YGGCCR 3 cut(s) 177, 378, 789
AcsI RAATTY 2 cut(s) 274, 359
AcyI GRCGYC 1 cut(s) 422
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 4 cut(s) 122, 713, 965, 993
AfiI CCNNNNNNNGG 5 cut(s) 191, 342, 439, 474, 680
AflIII ACRYGT 2 cut(s) 133, 981
AgeI ACCGGT 1 cut(s) 414
AgsI TTSAA 4 cut(s) 37, 748, 898, 1061
AjiI CACGTC 1 cut(s) 734
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 7 cut(s) 30, 289, 373, 386, 491, 758, 1040
AluI AGCT 7 cut(s) 30, 289, 373, 386, 491, 758, 1040
Alw26I GTCTC 2 cut(s) 136, 947
AlwI GGATC 2 cut(s) 17, 176
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 4 cut(s) 177, 378, 789, 1050
ApeKI GCWGC 3 cut(s) 727, 755, 923
ApoI RAATTY 2 cut(s) 274, 359
AsiGI ACCGGT 1 cut(s) 414
AspLEI GCGC 2 cut(s) 329, 424
AsuHPI GGTGA 2 cut(s) 7, 62
BaeGI GKGCMC 1 cut(s) 981
BanI GGYRCC 3 cut(s) 4, 421, 581
BanII GRGCYC 1 cut(s) 469
BarI GAAGNNNNNNTAC 2 cut(s) 701, 733
BauI CACGAG 1 cut(s) 838
BbvI GCAGC 3 cut(s) 739, 767, 910
BccI CCATC 4 cut(s) 72, 449, 816, 862
BceAI ACGGC 1 cut(s) 850
BciVI GTATCC 1 cut(s) 757
BclI TGATCA 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 136, 947
BfaI CTAG 4 cut(s) 552, 933, 995, 1069
BfoI RGCGCY 1 cut(s) 425
BfuI GTATCC 1 cut(s) 757
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 6 cut(s) 171, 378, 444, 728, 756, 924
BlsI GCNGC 6 cut(s) 172, 379, 445, 729, 757, 925
BmcAI AGTACT 1 cut(s) 713
BmgBI CACGTC 1 cut(s) 734
BmiI GGNNCC 5 cut(s) 6, 334, 423, 583, 796
BmsI GCATC 1 cut(s) 1013
BpmI CTGGAG 1 cut(s) 793
BsaHI GRCGYC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 204, 265, 812
BsaWI WCCGGW 2 cut(s) 184, 414
BsaXI ACNNNNNCTCC 2 cut(s) 899, 929
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 342, 439, 474, 680
Bse118I RCCGGY 3 cut(s) 414, 787, 791
Bse1I ACTGG 2 cut(s) 523, 776
Bse3DI GCAATG 1 cut(s) 933
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 204, 265, 812
BseGI GGATG 3 cut(s) 200, 276, 808
BseLI CCNNNNNNNGG 5 cut(s) 191, 342, 439, 474, 680
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 1 cut(s) 475
BseNI ACTGG 2 cut(s) 523, 776
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 981
BseX3I CGGCCG 1 cut(s) 789
BseXI GCAGC 3 cut(s) 739, 767, 910
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 2 cut(s) 804, 983
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 4 cut(s) 179, 380, 791, 1052
BshNI GGYRCC 3 cut(s) 4, 421, 581
BshTI ACCGGT 1 cut(s) 414
BsiEI CGRYCG 1 cut(s) 792
BsiSI CCGG 5 cut(s) 185, 415, 440, 788, 792
BslFI GGGAC 2 cut(s) 140, 640
BslI CCNNNNNNNGG 5 cut(s) 191, 342, 439, 474, 680
BsmAI GTCTC 2 cut(s) 136, 947
BsmFI GGGAC 2 cut(s) 140, 640
BsnI GGCC 4 cut(s) 179, 380, 791, 1052
Bso31I GGTCTC 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 469, 981
Bsp13I TCCGGA 1 cut(s) 184
Bsp1407I TGTACA 2 cut(s) 120, 963
Bsp143I GATC 5 cut(s) 22, 90, 150, 181, 603
Bsp19I CCATGG 2 cut(s) 204, 812
BspACI CCGC 6 cut(s) 146, 171, 339, 377, 443, 802
BspANI GGCC 4 cut(s) 179, 380, 791, 1052
BspCNI CTCAG 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 2 cut(s) 804, 983
BspLI GGNNCC 5 cut(s) 6, 334, 423, 583, 796
BspPI GGATC 2 cut(s) 17, 176
BspT107I GGYRCC 3 cut(s) 4, 421, 581
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 3 cut(s) 414, 787, 791
BsrGI TGTACA 2 cut(s) 120, 963
BsrI ACTGG 2 cut(s) 523, 776
BssAI RCCGGY 3 cut(s) 414, 787, 791
BssECI CCNNGG 3 cut(s) 204, 265, 812
BssMI GATC 5 cut(s) 22, 90, 150, 181, 603
BssNI GRCGYC 1 cut(s) 422
BssSI CACGAG 1 cut(s) 838
BssT1I CCWWGG 2 cut(s) 204, 812
Bst2BI CACGAG 1 cut(s) 838
Bst4CI ACNGT 2 cut(s) 58, 958
Bst6I CTCTTC 1 cut(s) 441
BstACI GRCGYC 1 cut(s) 422
BstAUI TGTACA 2 cut(s) 120, 963
BstC8I GCNNGC 7 cut(s) 9, 331, 375, 789, 940, 981, 1011
BstDEI CTNAG 2 cut(s) 19, 461
BstDSI CCRYGG 3 cut(s) 204, 265, 812
BstF5I GGATG 3 cut(s) 200, 276, 808
BstFNI CGCG 2 cut(s) 804, 983
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 2 cut(s) 329, 424
BstKTI GATC 5 cut(s) 25, 93, 153, 184, 606
BstMAI GTCTC 2 cut(s) 136, 947
BstMBI GATC 5 cut(s) 22, 90, 150, 181, 603
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 7 cut(s) 176, 295, 374, 383, 590, 788, 929
BstNSI RCATGY 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 981
BstUI CGCG 2 cut(s) 804, 983
BstV1I GCAGC 3 cut(s) 739, 767, 910
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BstZI CGGCCG 1 cut(s) 789
BsuI GTATCC 1 cut(s) 757
BsuRI GGCC 4 cut(s) 179, 380, 791, 1052
BtgI CCRYGG 3 cut(s) 204, 265, 812
BtrI CACGTC 1 cut(s) 734
BtsCI GGATG 3 cut(s) 200, 276, 808
BtsI GCAGTG 3 cut(s) 165, 545, 782
BtsIMutI CAGTG 4 cut(s) 165, 530, 545, 782
Cac8I GCNNGC 7 cut(s) 9, 331, 375, 789, 940, 981, 1011
CfoI GCGC 2 cut(s) 329, 424
Cfr10I RCCGGY 3 cut(s) 414, 787, 791
CseI GACGC 1 cut(s) 151
Csp6I GTAC 4 cut(s) 121, 712, 964, 992
CspAI ACCGGT 1 cut(s) 414
CviAII CATG 8 cut(s) 77, 134, 205, 356, 400, 721, 813, 1028
CviQI GTAC 4 cut(s) 121, 712, 964, 992
DdeI CTNAG 2 cut(s) 19, 461
DinI GGCGCC 1 cut(s) 423
DpnI GATC 5 cut(s) 24, 92, 152, 183, 605
DpnII GATC 5 cut(s) 22, 90, 150, 181, 603
DraIII CACNNNGTG 1 cut(s) 742
EaeI YGGCCR 3 cut(s) 177, 378, 789
EagI CGGCCG 1 cut(s) 789
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EclXI CGGCCG 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 204, 812
Eco147I AGGCCT 1 cut(s) 1052
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 1 cut(s) 136
Eco52I CGGCCG 1 cut(s) 789
EcoRI GAATTC 1 cut(s) 274
EcoT14I CCWWGG 2 cut(s) 204, 812
EcoT38I GRGCYC 1 cut(s) 469
EgeI GGCGCC 1 cut(s) 423
EheI GGCGCC 1 cut(s) 423
ErhI CCWWGG 2 cut(s) 204, 812
FaeI CATG 8 cut(s) 80, 137, 208, 359, 403, 724, 816, 1031
FaqI GGGAC 2 cut(s) 140, 640
FatI CATG 8 cut(s) 76, 133, 204, 355, 399, 720, 812, 1027
FbaI TGATCA 1 cut(s) 603
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 171, 378, 444, 728, 756, 924
FokI GGATG 3 cut(s) 187, 283, 795
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 6 cut(s) 171, 378, 444, 728, 756, 924
FspBI CTAG 4 cut(s) 552, 933, 995, 1069
GlaI GCGC 2 cut(s) 328, 423
GluI GCNGC 6 cut(s) 171, 378, 444, 728, 756, 924
GsuI CTGGAG 1 cut(s) 793
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 4 cut(s) 179, 380, 791, 1052
HapII CCGG 5 cut(s) 185, 415, 440, 788, 792
HgaI GACGC 1 cut(s) 151
HhaI GCGC 2 cut(s) 329, 424
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 8 cut(s) 80, 137, 208, 359, 403, 724, 816, 1031
Hin6I GCGC 2 cut(s) 327, 422
HinP1I GCGC 2 cut(s) 327, 422
HincII GTYRAC 2 cut(s) 61, 534
HindII GTYRAC 2 cut(s) 61, 534
HindIII AAGCTT 2 cut(s) 287, 489
HinfI GANTC 2 cut(s) 459, 748
HpaII CCGG 5 cut(s) 185, 415, 440, 788, 792
HphI GGTGA 2 cut(s) 7, 62
Hpy166II GTNNAC 6 cut(s) 61, 121, 228, 500, 534, 964
Hpy188I TCNGA 6 cut(s) 258, 464, 706, 763, 853, 883
Hpy188III TCNNGA 2 cut(s) 185, 552
Hpy8I GTNNAC 6 cut(s) 61, 121, 228, 500, 534, 964
HpyCH4III ACNGT 2 cut(s) 58, 958
HpyCH4IV ACGT 5 cut(s) 234, 244, 502, 733, 948
HpyCH4V TGCA 4 cut(s) 670, 727, 819, 926
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 295, 374, 383, 590, 788, 929
HpyF3I CTNAG 2 cut(s) 19, 461
HpySE526I ACGT 5 cut(s) 234, 244, 502, 733, 948
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 8 cut(s) 80, 137, 208, 359, 403, 724, 816, 1031
HspAI GCGC 2 cut(s) 327, 422
KasI GGCGCC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 184
KroI GCCGGC 1 cut(s) 787
KroNI GCCGGC 1 cut(s) 789
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 5 cut(s) 22, 90, 150, 181, 603
LmnI GCTCC 1 cut(s) 338
Lsp1109I GCAGC 3 cut(s) 739, 767, 910
LweI GCATC 1 cut(s) 1013
MaeI CTAG 4 cut(s) 552, 933, 995, 1069
MaeII ACGT 5 cut(s) 234, 244, 502, 733, 948
MaeIII GTNAC 4 cut(s) 163, 196, 350, 734
MalI GATC 5 cut(s) 24, 92, 152, 183, 605
MboI GATC 5 cut(s) 22, 90, 150, 181, 603
MboII GAAGA 3 cut(s) 295, 428, 464
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 2 cut(s) 469, 981
MluCI AATT 6 cut(s) 274, 359, 409, 630, 648, 660
MluI ACGCGT 1 cut(s) 981
Mly113I GGCGCC 1 cut(s) 422
MlyI GAGTC 1 cut(s) 453
MmeI TCCRAC 2 cut(s) 822, 867
MnlI CCTC 6 cut(s) 88, 250, 308, 444, 505, 962
MroI TCCGGA 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 787
MseI TTAA 3 cut(s) 644, 651, 1047
MslI CAYNNNNRTG 2 cut(s) 75, 315
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 5 cut(s) 185, 415, 440, 788, 792
MvnI CGCG 2 cut(s) 804, 983
MwoI GCNNNNNNNGC 7 cut(s) 176, 295, 374, 383, 590, 788, 929
NaeI GCCGGC 1 cut(s) 789
NarI GGCGCC 1 cut(s) 422
NcoI CCATGG 2 cut(s) 204, 812
NdeII GATC 5 cut(s) 22, 90, 150, 181, 603
NgoMIV GCCGGC 1 cut(s) 787
NlaIII CATG 8 cut(s) 80, 137, 208, 359, 403, 724, 816, 1031
NlaIV GGNNCC 5 cut(s) 6, 334, 423, 583, 796
NmuCI GTSAC 3 cut(s) 163, 196, 734
NspI RCATGY 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 1052
PciI ACATGT 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 789
PfeI GAWTC 1 cut(s) 748
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 2 cut(s) 342, 680
PinAI ACCGGT 1 cut(s) 414
PkrI GCNGC 6 cut(s) 172, 379, 445, 729, 757, 925
PleI GAGTC 1 cut(s) 453
PluTI GGCGCC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 453
PscI ACATGT 1 cut(s) 133
Psp1406I AACGTT 1 cut(s) 234
PspN4I GGNNCC 5 cut(s) 6, 334, 423, 583, 796
PsuI RGATCY 1 cut(s) 22
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 4 cut(s) 122, 713, 965, 993
RsaNI GTAC 4 cut(s) 121, 712, 964, 992
RseI CAYNNNNRTG 2 cut(s) 75, 315
SalI GTCGAC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 644, 651, 1047
SatI GCNGC 6 cut(s) 171, 378, 444, 728, 756, 924
Sau3AI GATC 5 cut(s) 22, 90, 150, 181, 603
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 1 cut(s) 453
SduI GDGCHC 2 cut(s) 469, 981
SfaNI GCATC 1 cut(s) 1013
SfoI GGCGCC 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 75, 315
Sse9I AATT 6 cut(s) 274, 359, 409, 630, 648, 660
SseBI AGGCCT 1 cut(s) 1052
SsiI CCGC 6 cut(s) 146, 171, 339, 377, 443, 802
SspDI GGCGCC 1 cut(s) 421
SspI AATATT 1 cut(s) 558
SspMI CTAG 4 cut(s) 552, 933, 995, 1069
StuI AGGCCT 1 cut(s) 1052
StyI CCWWGG 2 cut(s) 204, 812
TaaI ACNGT 2 cut(s) 58, 958
TaiI ACGT 5 cut(s) 237, 247, 505, 736, 951
TaqI TCGA 4 cut(s) 60, 93, 278, 1021
TasI AATT 6 cut(s) 274, 359, 409, 630, 648, 660
TatI WGTACW 3 cut(s) 120, 711, 963
TauI GCSGC 3 cut(s) 173, 380, 446
TfiI GAWTC 1 cut(s) 748
Tru1I TTAA 3 cut(s) 644, 651, 1047
Tru9I TTAA 3 cut(s) 644, 651, 1047
TscAI CASTG 4 cut(s) 172, 530, 545, 789
TseFI GTSAC 3 cut(s) 163, 196, 734
TseI GCWGC 3 cut(s) 727, 755, 923
Tsp45I GTSAC 3 cut(s) 163, 196, 734
TspDTI ATGAA 4 cut(s) 287, 296, 372, 465
TspGWI ACGGA 2 cut(s) 144, 327
TspRI CASTG 4 cut(s) 172, 530, 545, 789
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 2 cut(s) 342, 680
XapI RAATTY 2 cut(s) 274, 359
XbaI TCTAGA 1 cut(s) 551
XceI RCATGY 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 4 cut(s) 552, 933, 995, 1069
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.