Rw4G029840

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014973
Physical Location & Seq
Reverse (-)
56535684 .. 56536915
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029840.1

Sequence Viewer

Length: 1086 bp
ATGAGAACACAAAAAATGGTGCCTGCTATCACCTTAGATCCAAGCTATTTCAAGCAAAAGACCAACAACACATTCGATCATCATCACCAGCATGAGAACATTGTGATCGAGGAAATCAAAGGGCTTATCAAAGTGTACAAAGACGGACATGTGGAGAGACCGCAGATCGTCCCGTGCGTCACGGCCGCTGTGGCCGATCCGGAGTTAGGCGTGACATCCATGGACATAGTCATTGACAGGTTCACAAACGTTTCCTCACGTTTCTATGTTCCGAAACACCACGGGATGAATTCGATGAAGAAGCTTCCTTTGCTGGTGTATTTTCACGGAGGTGGGTTTTGCGCTGGCTCCACCGCTTGGAGTTGTTACCATGAATTTTTGGCAAAGCTAGCGGCCAAAGCTGGTTGCGTAATGATGTCGGTAAATTACCGGTTGGCACCCGAACACCCTCTTCCGGCGGCTTATGAAGATGGACTCAGAGCCCTTGTGTGGCTAAAACAACAAGCTTTTTGTGAACGTGGTGGGGAGGAGAATAACCAGTGGTGGTCAACGCAGTGCGACTTTTCTAGAATATTCTTGGTGGGGGATAGTGCTGGTGCCAATATAGCCCATAATGTGAGCACAAGACTGCTTTGTTCTGGGACTATTCTATTGCCTTTAATAATTAAAGGTGTAATTCTGGTGCAACCATTTTTTGGGGGAGAAGCAAGGACGAGTTCGGAGAAGTACTACTACATGGTGCAGCCACGTCATTCGGTGTTGAATCTGGCAGCTTCGGATACCTATTGGAGATTGGCACTGCCGGCCGGTTCCAACCGCGACCATCCATGGTGCAACCCTTTGGCTAACGGCTCGTGCTTCGGTTCGGATAGCGTGGCTATGATGGTGTGTATTTCGGAGTTGGACATATTGAAAGATAGGGAGTTAGAGTTTTGTGCTGCAATGGCTAGAGCAGGGAAGAACGTAGAGACTGTGGTGTACAGAGGTGTAGGGCACGCGTTTCAGGTACTAGATAAGTCTATGCTCGCACAAACTCGATGCCATGAGATGATAGCTCATATTAAGGCCTTTGTTGAAAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

361

Amino Acids

40.3

Weight (kDa)

7.61

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 89 - 146 5.1e-07 Carboxylesterase family
BD-FAE PF20434 97 - 204 8.3e-12 BD-FAE
Abhydrolase_3 PF07859 105 - 335 5e-61 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 19, 436, 596
AccB7I CCANNNNNTGG 2 cut(s) 357, 695
AccII CGCG 2 cut(s) 819, 998
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
AclI AACGTT 1 cut(s) 249
AclWI GGATC 2 cut(s) 32, 191
AcoI YGGCCR 4 cut(s) 183, 192, 393, 804
AcsI RAATTY 2 cut(s) 289, 374
AfaI GTAC 4 cut(s) 137, 728, 980, 1008
AfiI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
AflIII ACRYGT 2 cut(s) 148, 996
AgeI ACCGGT 1 cut(s) 429
AgsI TTSAA 4 cut(s) 52, 763, 913, 1076
AjiI CACGTC 1 cut(s) 749
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 7 cut(s) 45, 304, 388, 401, 506, 773, 1055
AluI AGCT 7 cut(s) 45, 304, 388, 401, 506, 773, 1055
Alw21I GWGCWC 1 cut(s) 623
Alw26I GTCTC 2 cut(s) 151, 962
AlwI GGATC 2 cut(s) 32, 191
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 5 cut(s) 183, 192, 393, 804, 1065
ApeKI GCWGC 3 cut(s) 742, 770, 938
ApoI RAATTY 2 cut(s) 289, 374
AsiGI ACCGGT 1 cut(s) 429
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 22, 77
AsuNHI GCTAGC 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 996
BanI GGYRCC 3 cut(s) 19, 436, 596
BanII GRGCYC 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
BauI CACGAG 1 cut(s) 853
Bbv12I GWGCWC 1 cut(s) 623
BbvI GCAGC 3 cut(s) 754, 782, 925
BccI CCATC 3 cut(s) 464, 831, 877
BceAI ACGGC 2 cut(s) 198, 865
BciVI GTATCC 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 151, 962
BfaI CTAG 5 cut(s) 389, 567, 948, 1010, 1084
BfuI GTATCC 1 cut(s) 772
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
BlsI GCNGC 6 cut(s) 187, 394, 460, 744, 772, 940
BmcAI AGTACT 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 749
BmiI GGNNCC 5 cut(s) 21, 349, 438, 598, 811
BmsI GCATC 1 cut(s) 1028
BmtI GCTAGC 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 219, 280, 827
BsaWI WCCGGW 2 cut(s) 199, 429
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
Bse118I RCCGGY 3 cut(s) 429, 802, 806
Bse1I ACTGG 1 cut(s) 538
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 81
BseAI TCCGGA 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 219, 280, 827
BseGI GGATG 3 cut(s) 215, 291, 823
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 1 cut(s) 490
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 996
BseX3I CGGCCG 2 cut(s) 183, 804
BseXI GCAGC 3 cut(s) 754, 782, 925
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 2 cut(s) 819, 998
Bsh1285I CGRYCG 2 cut(s) 186, 807
BshFI GGCC 5 cut(s) 185, 194, 395, 806, 1067
BshNI GGYRCC 3 cut(s) 19, 436, 596
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 186, 807
BsiHKAI GWGCWC 1 cut(s) 623
BsiSI CCGG 5 cut(s) 200, 430, 455, 803, 807
BslFI GGGAC 2 cut(s) 155, 655
BslI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
BsmAI GTCTC 2 cut(s) 151, 962
BsmFI GGGAC 2 cut(s) 155, 655
BsnI GGCC 5 cut(s) 185, 194, 395, 806, 1067
Bso31I GGTCTC 1 cut(s) 151
Bsp1286I GDGCHC 3 cut(s) 484, 623, 996
Bsp13I TCCGGA 1 cut(s) 199
Bsp1407I TGTACA 2 cut(s) 135, 978
Bsp143I GATC 5 cut(s) 37, 76, 105, 165, 196
Bsp19I CCATGG 2 cut(s) 219, 827
BspACI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
BspANI GGCC 5 cut(s) 185, 194, 395, 806, 1067
BspCNI CTCAG 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 199
BspFNI CGCG 2 cut(s) 819, 998
BspLI GGNNCC 5 cut(s) 21, 349, 438, 598, 811
BspOI GCTAGC 1 cut(s) 392
BspPI GGATC 2 cut(s) 32, 191
BspT107I GGYRCC 3 cut(s) 19, 436, 596
BspTNI GGTCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 3 cut(s) 429, 802, 806
BsrGI TGTACA 2 cut(s) 135, 978
BsrI ACTGG 1 cut(s) 538
BssAI RCCGGY 3 cut(s) 429, 802, 806
BssECI CCNNGG 3 cut(s) 219, 280, 827
BssMI GATC 5 cut(s) 37, 76, 105, 165, 196
BssSI CACGAG 1 cut(s) 853
BssT1I CCWWGG 2 cut(s) 219, 827
Bst2BI CACGAG 1 cut(s) 853
Bst4CI ACNGT 1 cut(s) 973
Bst6I CTCTTC 1 cut(s) 456
BstAUI TGTACA 2 cut(s) 135, 978
BstC8I GCNNGC 6 cut(s) 24, 346, 390, 804, 996, 1026
BstDEI CTNAG 2 cut(s) 34, 476
BstDSI CCRYGG 3 cut(s) 219, 280, 827
BstF5I GGATG 3 cut(s) 215, 291, 823
BstFNI CGCG 2 cut(s) 819, 998
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 5 cut(s) 40, 79, 108, 168, 199
BstMAI GTCTC 2 cut(s) 151, 962
BstMBI GATC 5 cut(s) 37, 76, 105, 165, 196
BstMCI CGRYCG 2 cut(s) 186, 807
BstMWI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
BstNSI RCATGY 1 cut(s) 152
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 2 cut(s) 819, 998
BstV1I GCAGC 3 cut(s) 754, 782, 925
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 2 cut(s) 183, 804
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 5 cut(s) 185, 194, 395, 806, 1067
BtgI CCRYGG 3 cut(s) 219, 280, 827
BtrI CACGTC 1 cut(s) 749
BtsCI GGATG 3 cut(s) 215, 291, 823
BtsI GCAGTG 2 cut(s) 560, 797
BtsIMutI CAGTG 3 cut(s) 545, 560, 797
Cac8I GCNNGC 6 cut(s) 24, 346, 390, 804, 996, 1026
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 3 cut(s) 429, 802, 806
CseI GACGC 1 cut(s) 166
Csp6I GTAC 4 cut(s) 136, 727, 979, 1007
CspAI ACCGGT 1 cut(s) 429
CviAII CATG 7 cut(s) 92, 149, 220, 371, 736, 828, 1043
CviQI GTAC 4 cut(s) 136, 727, 979, 1007
DdeI CTNAG 2 cut(s) 34, 476
DpnI GATC 5 cut(s) 39, 78, 107, 167, 198
DpnII GATC 5 cut(s) 37, 76, 105, 165, 196
EaeI YGGCCR 4 cut(s) 183, 192, 393, 804
EagI CGGCCG 2 cut(s) 183, 804
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EclXI CGGCCG 2 cut(s) 183, 804
Eco130I CCWWGG 2 cut(s) 219, 827
Eco147I AGGCCT 1 cut(s) 1067
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 151
Eco52I CGGCCG 2 cut(s) 183, 804
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 219, 827
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 2 cut(s) 219, 827
FaeI CATG 7 cut(s) 95, 152, 223, 374, 739, 831, 1046
FaqI GGGAC 2 cut(s) 155, 655
FatI CATG 7 cut(s) 91, 148, 219, 370, 735, 827, 1042
Fnu4HI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
FokI GGATG 3 cut(s) 202, 298, 810
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
FspBI CTAG 5 cut(s) 389, 567, 948, 1010, 1084
GlaI GCGC 1 cut(s) 343
GluI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
HaeIII GGCC 5 cut(s) 185, 194, 395, 806, 1067
HapII CCGG 5 cut(s) 200, 430, 455, 803, 807
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 344
Hin1II CATG 7 cut(s) 95, 152, 223, 374, 739, 831, 1046
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 2 cut(s) 302, 504
HinfI GANTC 2 cut(s) 474, 763
HpaII CCGG 5 cut(s) 200, 430, 455, 803, 807
HphI GGTGA 2 cut(s) 22, 77
Hpy166II GTNNAC 5 cut(s) 136, 243, 515, 549, 979
Hpy188I TCNGA 6 cut(s) 273, 479, 721, 778, 868, 898
Hpy188III TCNNGA 2 cut(s) 200, 567
Hpy8I GTNNAC 5 cut(s) 136, 243, 515, 549, 979
HpyCH4III ACNGT 1 cut(s) 973
HpyCH4IV ACGT 5 cut(s) 249, 259, 517, 748, 963
HpyCH4V TGCA 4 cut(s) 685, 742, 834, 941
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
HpyF3I CTNAG 2 cut(s) 34, 476
HpySE526I ACGT 5 cut(s) 249, 259, 517, 748, 963
Hsp92II CATG 7 cut(s) 95, 152, 223, 374, 739, 831, 1046
HspAI GCGC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 199
KroI GCCGGC 1 cut(s) 802
KroNI GCCGGC 1 cut(s) 804
Kzo9I GATC 5 cut(s) 37, 76, 105, 165, 196
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 3 cut(s) 754, 782, 925
LweI GCATC 1 cut(s) 1028
MaeI CTAG 5 cut(s) 389, 567, 948, 1010, 1084
MaeII ACGT 5 cut(s) 249, 259, 517, 748, 963
MaeIII GTNAC 3 cut(s) 178, 211, 365
MalI GATC 5 cut(s) 39, 78, 107, 167, 198
MboI GATC 5 cut(s) 37, 76, 105, 165, 196
MboII GAAGA 4 cut(s) 310, 443, 479, 970
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 3 cut(s) 484, 623, 996
MluCI AATT 5 cut(s) 289, 374, 424, 663, 675
MluI ACGCGT 1 cut(s) 996
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 2 cut(s) 837, 882
MnlI CCTC 6 cut(s) 103, 265, 323, 459, 520, 977
MroI TCCGGA 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 802
MseI TTAA 3 cut(s) 659, 666, 1062
MslI CAYNNNNRTG 2 cut(s) 90, 330
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 5 cut(s) 200, 430, 455, 803, 807
MvnI CGCG 2 cut(s) 819, 998
MwoI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
NaeI GCCGGC 1 cut(s) 804
NcoI CCATGG 2 cut(s) 219, 827
NdeII GATC 5 cut(s) 37, 76, 105, 165, 196
NgoMIV GCCGGC 1 cut(s) 802
NheI GCTAGC 1 cut(s) 388
NlaIII CATG 7 cut(s) 95, 152, 223, 374, 739, 831, 1046
NlaIV GGNNCC 5 cut(s) 21, 349, 438, 598, 811
NmuCI GTSAC 2 cut(s) 178, 211
NspI RCATGY 1 cut(s) 152
OliI CACNNNNGTG 1 cut(s) 330
PceI AGGCCT 1 cut(s) 1067
PciI ACATGT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 804
PfeI GAWTC 1 cut(s) 763
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 2 cut(s) 357, 695
PinAI ACCGGT 1 cut(s) 429
PkrI GCNGC 6 cut(s) 187, 394, 460, 744, 772, 940
PleI GAGTC 1 cut(s) 468
PpsI GAGTC 1 cut(s) 468
PscI ACATGT 1 cut(s) 148
Psp1406I AACGTT 1 cut(s) 249
PspN4I GGNNCC 5 cut(s) 21, 349, 438, 598, 811
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 4 cut(s) 137, 728, 980, 1008
RsaNI GTAC 4 cut(s) 136, 727, 979, 1007
RseI CAYNNNNRTG 2 cut(s) 90, 330
SaqAI TTAA 3 cut(s) 659, 666, 1062
SatI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
Sau3AI GATC 5 cut(s) 37, 76, 105, 165, 196
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 1 cut(s) 468
SduI GDGCHC 3 cut(s) 484, 623, 996
SfaNI GCATC 1 cut(s) 1028
SfiI GGCCNNNNNGGCC 1 cut(s) 191
SmiMI CAYNNNNRTG 2 cut(s) 90, 330
Sse9I AATT 5 cut(s) 289, 374, 424, 663, 675
SseBI AGGCCT 1 cut(s) 1067
SsiI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
SspI AATATT 1 cut(s) 573
SspMI CTAG 5 cut(s) 389, 567, 948, 1010, 1084
StuI AGGCCT 1 cut(s) 1067
StyI CCWWGG 2 cut(s) 219, 827
TaaI ACNGT 1 cut(s) 973
TaiI ACGT 5 cut(s) 252, 262, 520, 751, 966
TaqI TCGA 4 cut(s) 75, 108, 293, 1036
TasI AATT 5 cut(s) 289, 374, 424, 663, 675
TatI WGTACW 3 cut(s) 135, 726, 978
TauI GCSGC 3 cut(s) 188, 395, 461
TfiI GAWTC 1 cut(s) 763
Tru1I TTAA 3 cut(s) 659, 666, 1062
Tru9I TTAA 3 cut(s) 659, 666, 1062
TscAI CASTG 3 cut(s) 545, 560, 804
TseFI GTSAC 2 cut(s) 178, 211
TseI GCWGC 3 cut(s) 742, 770, 938
Tsp45I GTSAC 2 cut(s) 178, 211
TspDTI ATGAA 4 cut(s) 302, 311, 387, 480
TspGWI ACGGA 2 cut(s) 159, 342
TspRI CASTG 3 cut(s) 545, 560, 804
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 2 cut(s) 357, 695
XapI RAATTY 2 cut(s) 289, 374
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 1 cut(s) 152
XspI CTAG 5 cut(s) 389, 567, 948, 1010, 1084
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.