Rroxscaffold_5G00376800

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014973
Physical Location & Seq
Reverse (-)
57402776 .. 57405574
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376800.1

Sequence Viewer

Length: 1113 bp
ATGCTCGATCTGCTTCAACTTAATTGGGTGGATGATGTCCCAGACAGTTCCCTAGCTCCAAGAGATCCAAGCTATTTCAAGCAAAAGATCAACAACACAGTCGACCATCATCACCAGCATGAGAACATTGTGATCGAGGAAATCAAAGGGCTTATCAAAGTGTACAAAGACGGACATGTGGAGAGACCGCAGATCGTCCCGTGCGTCACGGCCTCTGTGGCCGATCTGGAGTTAGGCGTGACATCCATGGACATAGTCATTGACAGATTCACAAACGTTTCCTCACGTTTCTATGTTCCGAAACGCCGCGGAATGAATTCGATGAAGAAGCTCCCTTTGCTGGTGTATTTTCACGGAGGTGGGTTCTGCGCTGGCTCCACCGCTTGGAGTTGTTACCATGAATTTTTGGCAAAGCTAGCGGCCAAAGCTGGTTGCGTAATCATGTCGGTAAATTACCGGTTGGCCCCCGAACACCCTCTTCCGGCGGCTTATGAAGATGGACTCAGAGCCCTTGTGTGGCTAAAACAACAAGCTTTTTGTGGACGTGGTGGGGAGGAGAATAACCAGTGGTGGTCAACACAGTGCGACTTTTCTAGAATATTCTTGGTGGGGGATAGTGCTGGTGCCAATATAGCCCATAATGTGAGCACAAGACTGCTTTGTTCTGGGACAATTCTATTGCCTTTAATAATTAAAGGTGTAATTCTGGTGCAACCATTTTTTGGGGGAGAAGCAAGGACGAGTTCGGAGAAGTACTACTACATGGTGCAGCCACGTCACTCGGTGTTGAATCTGGCAGCTTCGGATACCTACTGGAGATTGGCACTGCCGGCCGGTTCCAACCGCGACCATCCATGGTGCAACCCTTTGGCTAACGGCTCGTGTTTCGGTTCGGATAGCGTGGCGATGATGGTGTGTATTTCGGAGTTGGACATATTGAAAGATAGGGAGTTAGAGTTTTGTGCTGCAATCGCTAGAGCAGGGAAGAACGTGGAGACTGTGGTGTACAGAGGTGTAGGGCACGCGTTTCAGGTACTAGATAAGTCTATGCTCGCACAAACTCGATGCCATGAGATGATAGCTCATATTAAGGCCTTTGTTGAAAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

370

Amino Acids

41.23

Weight (kDa)

6.91

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 98 - 155 4.5e-07 Carboxylesterase family
BD-FAE PF20434 106 - 213 6.7e-12 BD-FAE
Abhydrolase_3 PF07859 114 - 344 4.9e-61 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 2 cut(s) 384, 722
AccI GTMKAC 1 cut(s) 102
AccII CGCG 3 cut(s) 309, 846, 1025
AciI CCGC 7 cut(s) 188, 307, 309, 381, 419, 485, 844
AclI AACGTT 1 cut(s) 276
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 3 cut(s) 219, 420, 831
AcsI RAATTY 2 cut(s) 316, 401
AdeI CACNNNGTG 1 cut(s) 784
AfaI GTAC 4 cut(s) 164, 755, 1007, 1035
AfiI CCNNNNNNNGG 5 cut(s) 340, 384, 481, 516, 722
AflIII ACRYGT 2 cut(s) 175, 1023
AgeI ACCGGT 1 cut(s) 456
AgsI TTSAA 5 cut(s) 17, 79, 790, 940, 1103
AjiI CACGTC 2 cut(s) 545, 776
AleI CACNNNNGTG 1 cut(s) 357
AluBI AGCT 8 cut(s) 56, 72, 331, 415, 428, 533, 800, 1082
AluI AGCT 8 cut(s) 56, 72, 331, 415, 428, 533, 800, 1082
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 2 cut(s) 178, 989
AlwI GGATC 1 cut(s) 59
AoxI GGCC 6 cut(s) 210, 219, 420, 462, 831, 1092
ApeKI GCWGC 3 cut(s) 769, 797, 965
ApoI RAATTY 2 cut(s) 316, 401
AsiGI ACCGGT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 415
BaeGI GKGCMC 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 511
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BauI CACGAG 1 cut(s) 880
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 3 cut(s) 781, 809, 952
BccI CCATC 4 cut(s) 114, 491, 858, 904
BceAI ACGGC 2 cut(s) 225, 892
BciVI GTATCC 1 cut(s) 799
BcoDI GTCTC 2 cut(s) 178, 989
BfaI CTAG 6 cut(s) 53, 416, 594, 975, 1037, 1111
BfuI GTATCC 1 cut(s) 799
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 6 cut(s) 307, 420, 486, 770, 798, 966
BlsI GCNGC 6 cut(s) 308, 421, 487, 771, 799, 967
BmcAI AGTACT 1 cut(s) 755
BmgBI CACGTC 2 cut(s) 545, 776
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 4 cut(s) 376, 465, 625, 838
BmsI GCATC 1 cut(s) 1055
BmtI GCTAGC 1 cut(s) 419
BpmI CTGGAG 2 cut(s) 248, 835
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 246, 307, 854
BsaWI WCCGGW 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 384, 481, 516, 722
Bse118I RCCGGY 3 cut(s) 456, 829, 833
Bse1I ACTGG 2 cut(s) 565, 818
BseDI CCNNGG 3 cut(s) 246, 307, 854
BseGI GGATG 3 cut(s) 37, 242, 850
BseLI CCNNNNNNNGG 5 cut(s) 340, 384, 481, 516, 722
BseMII CTCAG 1 cut(s) 517
BseNI ACTGG 2 cut(s) 565, 818
BseRI GAGGAG 1 cut(s) 569
BseSI GKGCMC 1 cut(s) 1023
BseX3I CGGCCG 1 cut(s) 831
BseXI GCAGC 3 cut(s) 781, 809, 952
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 3 cut(s) 309, 846, 1025
Bsh1285I CGRYCG 1 cut(s) 834
BshFI GGCC 6 cut(s) 212, 221, 422, 464, 833, 1094
BshNI GGYRCC 1 cut(s) 623
BshTI ACCGGT 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 834
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 4 cut(s) 457, 482, 830, 834
BslFI GGGAC 3 cut(s) 23, 182, 682
BslI CCNNNNNNNGG 5 cut(s) 340, 384, 481, 516, 722
BsmAI GTCTC 2 cut(s) 178, 989
BsmFI GGGAC 3 cut(s) 23, 182, 682
BsnI GGCC 6 cut(s) 212, 221, 422, 464, 833, 1094
Bso31I GGTCTC 1 cut(s) 178
Bsp1286I GDGCHC 3 cut(s) 511, 650, 1023
Bsp1407I TGTACA 2 cut(s) 162, 1005
Bsp143I GATC 6 cut(s) 7, 64, 87, 132, 192, 223
Bsp19I CCATGG 2 cut(s) 246, 854
BspACI CCGC 7 cut(s) 188, 307, 309, 381, 419, 485, 844
BspANI GGCC 6 cut(s) 212, 221, 422, 464, 833, 1094
BspCNI CTCAG 1 cut(s) 516
BspFNI CGCG 3 cut(s) 309, 846, 1025
BspLI GGNNCC 4 cut(s) 376, 465, 625, 838
BspOI GCTAGC 1 cut(s) 419
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 178
BsrFI RCCGGY 3 cut(s) 456, 829, 833
BsrGI TGTACA 2 cut(s) 162, 1005
BsrI ACTGG 2 cut(s) 565, 818
BssAI RCCGGY 3 cut(s) 456, 829, 833
BssECI CCNNGG 3 cut(s) 246, 307, 854
BssMI GATC 6 cut(s) 7, 64, 87, 132, 192, 223
BssSI CACGAG 1 cut(s) 880
BssT1I CCWWGG 2 cut(s) 246, 854
Bst2BI CACGAG 1 cut(s) 880
Bst4CI ACNGT 4 cut(s) 47, 100, 582, 1000
Bst6I CTCTTC 1 cut(s) 483
BstAUI TGTACA 2 cut(s) 162, 1005
BstC8I GCNNGC 5 cut(s) 373, 417, 831, 1023, 1053
BstDEI CTNAG 1 cut(s) 503
BstDSI CCRYGG 3 cut(s) 246, 307, 854
BstF5I GGATG 3 cut(s) 37, 242, 850
BstFNI CGCG 3 cut(s) 309, 846, 1025
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 6 cut(s) 10, 67, 90, 135, 195, 226
BstMAI GTCTC 2 cut(s) 178, 989
BstMBI GATC 6 cut(s) 7, 64, 87, 132, 192, 223
BstMCI CGRYCG 1 cut(s) 834
BstMWI GCNNNNNNNGC 8 cut(s) 10, 218, 337, 416, 425, 632, 830, 971
BstNSI RCATGY 1 cut(s) 179
BstSLI GKGCMC 1 cut(s) 1023
BstUI CGCG 3 cut(s) 309, 846, 1025
BstV1I GCAGC 3 cut(s) 781, 809, 952
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BstZI CGGCCG 1 cut(s) 831
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 6 cut(s) 212, 221, 422, 464, 833, 1094
BtgI CCRYGG 3 cut(s) 246, 307, 854
BtgZI GCGATG 1 cut(s) 920
BtrI CACGTC 2 cut(s) 545, 776
BtsCI GGATG 3 cut(s) 37, 242, 850
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 3 cut(s) 572, 587, 824
Cac8I GCNNGC 5 cut(s) 373, 417, 831, 1023, 1053
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 3 cut(s) 456, 829, 833
Cfr13I GGNCC 1 cut(s) 463
Cfr42I CCGCGG 1 cut(s) 310
CseI GACGC 1 cut(s) 193
Csp6I GTAC 4 cut(s) 163, 754, 1006, 1034
CspAI ACCGGT 1 cut(s) 456
CviAII CATG 8 cut(s) 119, 176, 247, 398, 442, 763, 855, 1070
CviQI GTAC 4 cut(s) 163, 754, 1006, 1034
DdeI CTNAG 1 cut(s) 503
DpnI GATC 6 cut(s) 9, 66, 89, 134, 194, 225
DpnII GATC 6 cut(s) 7, 64, 87, 132, 192, 223
DraIII CACNNNGTG 1 cut(s) 784
EaeI YGGCCR 3 cut(s) 219, 420, 831
EagI CGGCCG 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EclXI CGGCCG 1 cut(s) 831
Eco130I CCWWGG 2 cut(s) 246, 854
Eco147I AGGCCT 1 cut(s) 1094
Eco24I GRGCYC 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 178
Eco52I CGGCCG 1 cut(s) 831
EcoRI GAATTC 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 246, 854
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 2 cut(s) 246, 854
FaeI CATG 8 cut(s) 122, 179, 250, 401, 445, 766, 858, 1073
FaqI GGGAC 3 cut(s) 23, 182, 682
FatI CATG 8 cut(s) 118, 175, 246, 397, 441, 762, 854, 1069
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 307, 420, 486, 770, 798, 966
FokI GGATG 3 cut(s) 44, 229, 837
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 6 cut(s) 307, 420, 486, 770, 798, 966
FspBI CTAG 6 cut(s) 53, 416, 594, 975, 1037, 1111
GlaI GCGC 1 cut(s) 370
GluI GCNGC 6 cut(s) 307, 420, 486, 770, 798, 966
GsuI CTGGAG 2 cut(s) 248, 835
HaeIII GGCC 6 cut(s) 212, 221, 422, 464, 833, 1094
HapII CCGG 4 cut(s) 457, 482, 830, 834
HgaI GACGC 1 cut(s) 193
HhaI GCGC 1 cut(s) 371
Hin1II CATG 8 cut(s) 122, 179, 250, 401, 445, 766, 858, 1073
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 2 cut(s) 103, 576
HindII GTYRAC 2 cut(s) 103, 576
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 3 cut(s) 267, 501, 790
HpaII CCGG 4 cut(s) 457, 482, 830, 834
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 5 cut(s) 103, 163, 542, 576, 1006
Hpy188I TCNGA 6 cut(s) 300, 506, 748, 805, 895, 925
Hpy188III TCNNGA 2 cut(s) 227, 594
Hpy8I GTNNAC 5 cut(s) 103, 163, 542, 576, 1006
HpyCH4III ACNGT 4 cut(s) 47, 100, 582, 1000
HpyCH4IV ACGT 5 cut(s) 276, 286, 544, 775, 990
HpyCH4V TGCA 4 cut(s) 712, 769, 861, 968
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 218, 337, 416, 425, 632, 830, 971
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 5 cut(s) 276, 286, 544, 775, 990
Hsp92II CATG 8 cut(s) 122, 179, 250, 401, 445, 766, 858, 1073
HspAI GCGC 1 cut(s) 369
KroI GCCGGC 1 cut(s) 829
KroNI GCCGGC 1 cut(s) 831
KspI CCGCGG 1 cut(s) 310
Kzo9I GATC 6 cut(s) 7, 64, 87, 132, 192, 223
LmnI GCTCC 3 cut(s) 61, 336, 380
Lsp1109I GCAGC 3 cut(s) 781, 809, 952
LweI GCATC 1 cut(s) 1055
MaeI CTAG 6 cut(s) 53, 416, 594, 975, 1037, 1111
MaeII ACGT 5 cut(s) 276, 286, 544, 775, 990
MaeIII GTNAC 4 cut(s) 205, 238, 392, 776
MalI GATC 6 cut(s) 9, 66, 89, 134, 194, 225
MboI GATC 6 cut(s) 7, 64, 87, 132, 192, 223
MboII GAAGA 4 cut(s) 337, 470, 506, 997
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 3 cut(s) 511, 650, 1023
MluCI AATT 7 cut(s) 22, 316, 401, 451, 672, 690, 702
MluI ACGCGT 1 cut(s) 1023
MlyI GAGTC 1 cut(s) 495
MmeI TCCRAC 2 cut(s) 864, 909
MnlI CCTC 7 cut(s) 130, 223, 292, 350, 486, 547, 1004
MroNI GCCGGC 1 cut(s) 829
MroXI GAANNNNTTC 1 cut(s) 316
MseI TTAA 4 cut(s) 21, 686, 693, 1089
MslI CAYNNNNRTG 2 cut(s) 117, 357
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 4 cut(s) 457, 482, 830, 834
MvnI CGCG 3 cut(s) 309, 846, 1025
MwoI GCNNNNNNNGC 8 cut(s) 10, 218, 337, 416, 425, 632, 830, 971
NaeI GCCGGC 1 cut(s) 831
NcoI CCATGG 2 cut(s) 246, 854
NdeII GATC 6 cut(s) 7, 64, 87, 132, 192, 223
NgoMIV GCCGGC 1 cut(s) 829
NheI GCTAGC 1 cut(s) 415
NlaIII CATG 8 cut(s) 122, 179, 250, 401, 445, 766, 858, 1073
NlaIV GGNNCC 4 cut(s) 376, 465, 625, 838
NmuCI GTSAC 3 cut(s) 205, 238, 776
NspI RCATGY 1 cut(s) 179
OliI CACNNNNGTG 1 cut(s) 357
PceI AGGCCT 1 cut(s) 1094
PciI ACATGT 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 267, 790
PflFI GACNNNGTC 1 cut(s) 254
PflMI CCANNNNNTGG 2 cut(s) 384, 722
PinAI ACCGGT 1 cut(s) 456
PkrI GCNGC 6 cut(s) 308, 421, 487, 771, 799, 967
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PscI ACATGT 1 cut(s) 175
Psp1406I AACGTT 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 376, 465, 625, 838
PspPI GGNCC 1 cut(s) 463
PsuI RGATCY 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 4 cut(s) 164, 755, 1007, 1035
RsaNI GTAC 4 cut(s) 163, 754, 1006, 1034
RseI CAYNNNNRTG 2 cut(s) 117, 357
SacII CCGCGG 1 cut(s) 310
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 4 cut(s) 21, 686, 693, 1089
SatI GCNGC 6 cut(s) 307, 420, 486, 770, 798, 966
Sau3AI GATC 6 cut(s) 7, 64, 87, 132, 192, 223
Sau96I GGNCC 1 cut(s) 463
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 1 cut(s) 495
SduI GDGCHC 3 cut(s) 511, 650, 1023
SfaNI GCATC 1 cut(s) 1055
SfiI GGCCNNNNNGGCC 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 310
SgrBI CCGCGG 1 cut(s) 310
SmiMI CAYNNNNRTG 2 cut(s) 117, 357
Sse9I AATT 7 cut(s) 22, 316, 401, 451, 672, 690, 702
SseBI AGGCCT 1 cut(s) 1094
SsiI CCGC 7 cut(s) 188, 307, 309, 381, 419, 485, 844
SspI AATATT 1 cut(s) 600
SspMI CTAG 6 cut(s) 53, 416, 594, 975, 1037, 1111
StuI AGGCCT 1 cut(s) 1094
StyI CCWWGG 2 cut(s) 246, 854
TaaI ACNGT 4 cut(s) 47, 100, 582, 1000
TaiI ACGT 5 cut(s) 279, 289, 547, 778, 993
TaqI TCGA 5 cut(s) 6, 102, 135, 320, 1063
TasI AATT 7 cut(s) 22, 316, 401, 451, 672, 690, 702
TatI WGTACW 3 cut(s) 162, 753, 1005
TauI GCSGC 3 cut(s) 309, 422, 488
TfiI GAWTC 2 cut(s) 267, 790
Tru1I TTAA 4 cut(s) 21, 686, 693, 1089
Tru9I TTAA 4 cut(s) 21, 686, 693, 1089
TscAI CASTG 3 cut(s) 572, 587, 831
TseFI GTSAC 3 cut(s) 205, 238, 776
TseI GCWGC 3 cut(s) 769, 797, 965
Tsp45I GTSAC 3 cut(s) 205, 238, 776
TspDTI ATGAA 4 cut(s) 329, 338, 414, 507
TspGWI ACGGA 2 cut(s) 186, 369
TspRI CASTG 3 cut(s) 572, 587, 831
Tth111I GACNNNGTC 1 cut(s) 254
Van91I CCANNNNNTGG 2 cut(s) 384, 722
XapI RAATTY 2 cut(s) 316, 401
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 316
XspI CTAG 6 cut(s) 53, 416, 594, 975, 1037, 1111
ZrmI AGTACT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.