Rh4DG344600

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014973
Physical Location & Seq
Reverse (-)
56654124 .. 56656853
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG344600.1

Sequence Viewer

Length: 1086 bp
ATGAGAACACAAAAAATGGTGCCTGCTATCACCTTAGATCCAAGCTATTTCAAGCAAAAGACCAACAACACAGTCGACCATCATCACCAGCATGAGAACATTGTGATCGAGGAAATCAAAGGGCTTATCAAAGTGTACAAAGACGGACATGTGGAGAGACCGCAGATCGTCCCGTGCGTCACTGCCGCTGTGGCCGATCCGGAGTTAGGCGTGACATCCATGGACATAGTCATTGACAGGTTCACAAACGTTTCCTCACGTTTCTATGTTCCGAAACGCCACGGGATGAATTCGATGAAGAAGCTTCCTTTGCTGGTGTATTTTCACGGAGGTGGGTTTTGCGCTGGCTCCACCGCTTGGAGTTGTTACCATGAATTTTTGGCAAAGCTGGCGGCCAAAGCTGGTTGCGTAATCATGTCGGTAAATTACCGGTTGGCGCCCGAACACCCTCTTCCGGCGGCTTATGAAGATGGACTCAGAGCCCTTGTGTGGCTAAAACAACAAGCTTTTTGTGAACGTGGTGGGGAGGAGAATAACCAGTGGTGGTCAACGCAGTGCGACTTTTCTAGAATATTCTTGGTGGGGGATAGTGCTGGTGCCAATATAGCCCATAATGTGATCACAAGACTGCTTTGTTCTGGGACAATTCTATTGCCTTTAATAATTAAAGGTGTAATTCTGGTGCAACCATTTTTTGGGGGAGAAGCAAGGACGAGTTCGGAGAAGTACTACTACATGGTGCAGCCACGTCACTCGGTGTTGAATCTGGCAGCTTCGGATACCTACTGGAGATTGGCACTGCCGGCCGGTTCCAACCGCGACCATCCATGGTGCAACCCTTTGGCTAACGGCTCGTGCTTCGGTTCGGATAGCGTGGCTATGATGGTGTGTATTTCGGAGTTGGACATATTGAAAGATAGGGAGTTAGAGTTTTGTGCTGCAATGGCTAGAGCAGGCAAGAACGTAGAGACTGTGGTGTACAGAGGTGTAGGGCACGCGTTTCAGGTACTAGATAAGTCTATGCTCGCACAAACTCGATGCCATGAGATGATAGCTCATATTAAGGCCTTTGTTGAAAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

361

Amino Acids

40.28

Weight (kDa)

7.96

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 89 - 146 4.6e-07 Carboxylesterase family
BD-FAE PF20434 97 - 203 7.7e-12 BD-FAE
Abhydrolase_3 PF07859 105 - 335 3.5e-61 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68620
fragaria_vesca FvH4_4g27710
malus_domestica MD16G1045600.v1.1
prunus_persica Prupe.1G307700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435571
rosa_laevigata RLG00000006581
rosa_multiflora Rmu_co8055184.1_g000001 Rmu_sc0001940.1_g000043 Rmu_sc0006697.1_g000007
rosa_roxburghii Rroxscaffold_5G00376800
rosa_rugosa Rorug04G0286700.1
rosa_samantha Rh4AG342100 Rh4BG350300 Rh4CG365000 Rh4DG344600
rosa_wichuraiana Rw4G029840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 19, 436, 596
AccB7I CCANNNNNTGG 2 cut(s) 357, 695
AccI GTMKAC 1 cut(s) 75
AccII CGCG 2 cut(s) 819, 998
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
AclI AACGTT 1 cut(s) 249
AclWI GGATC 2 cut(s) 32, 191
AcoI YGGCCR 3 cut(s) 192, 393, 804
AcsI RAATTY 2 cut(s) 289, 374
AcyI GRCGYC 1 cut(s) 437
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 4 cut(s) 137, 728, 980, 1008
AfiI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
AflIII ACRYGT 2 cut(s) 148, 996
AgeI ACCGGT 1 cut(s) 429
AgsI TTSAA 4 cut(s) 52, 763, 913, 1076
AjiI CACGTC 1 cut(s) 749
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 7 cut(s) 45, 304, 388, 401, 506, 773, 1055
AluI AGCT 7 cut(s) 45, 304, 388, 401, 506, 773, 1055
Alw26I GTCTC 2 cut(s) 151, 962
AlwI GGATC 2 cut(s) 32, 191
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 4 cut(s) 192, 393, 804, 1065
ApeKI GCWGC 3 cut(s) 742, 770, 938
ApoI RAATTY 2 cut(s) 289, 374
AsiGI ACCGGT 1 cut(s) 429
AspLEI GCGC 2 cut(s) 344, 439
AsuHPI GGTGA 2 cut(s) 22, 77
BaeGI GKGCMC 1 cut(s) 996
BanI GGYRCC 3 cut(s) 19, 436, 596
BanII GRGCYC 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
BauI CACGAG 1 cut(s) 853
BbvI GCAGC 3 cut(s) 754, 782, 925
BccI CCATC 4 cut(s) 87, 464, 831, 877
BceAI ACGGC 1 cut(s) 865
BciVI GTATCC 1 cut(s) 772
BclI TGATCA 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 151, 962
BfaI CTAG 4 cut(s) 567, 948, 1010, 1084
BfoI RGCGCY 1 cut(s) 440
BfuI GTATCC 1 cut(s) 772
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
BlsI GCNGC 6 cut(s) 187, 394, 460, 744, 772, 940
BmcAI AGTACT 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 749
BmiI GGNNCC 5 cut(s) 21, 349, 438, 598, 811
BmsI GCATC 1 cut(s) 1028
BpmI CTGGAG 1 cut(s) 808
BsaHI GRCGYC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 219, 280, 827
BsaWI WCCGGW 2 cut(s) 199, 429
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
Bse118I RCCGGY 3 cut(s) 429, 802, 806
Bse1I ACTGG 2 cut(s) 538, 791
Bse3DI GCAATG 1 cut(s) 948
BseAI TCCGGA 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 219, 280, 827
BseGI GGATG 3 cut(s) 215, 291, 823
BseLI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 1 cut(s) 490
BseNI ACTGG 2 cut(s) 538, 791
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 996
BseX3I CGGCCG 1 cut(s) 804
BseXI GCAGC 3 cut(s) 754, 782, 925
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 2 cut(s) 819, 998
Bsh1285I CGRYCG 1 cut(s) 807
BshFI GGCC 4 cut(s) 194, 395, 806, 1067
BshNI GGYRCC 3 cut(s) 19, 436, 596
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 1 cut(s) 807
BsiSI CCGG 5 cut(s) 200, 430, 455, 803, 807
BslFI GGGAC 2 cut(s) 155, 655
BslI CCNNNNNNNGG 5 cut(s) 206, 357, 454, 489, 695
BsmAI GTCTC 2 cut(s) 151, 962
BsmFI GGGAC 2 cut(s) 155, 655
BsnI GGCC 4 cut(s) 194, 395, 806, 1067
Bso31I GGTCTC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 484, 996
Bsp13I TCCGGA 1 cut(s) 199
Bsp1407I TGTACA 2 cut(s) 135, 978
Bsp143I GATC 5 cut(s) 37, 105, 165, 196, 618
Bsp19I CCATGG 2 cut(s) 219, 827
BspACI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
BspANI GGCC 4 cut(s) 194, 395, 806, 1067
BspCNI CTCAG 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 199
BspFNI CGCG 2 cut(s) 819, 998
BspLI GGNNCC 5 cut(s) 21, 349, 438, 598, 811
BspPI GGATC 2 cut(s) 32, 191
BspT107I GGYRCC 3 cut(s) 19, 436, 596
BspTNI GGTCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 3 cut(s) 429, 802, 806
BsrGI TGTACA 2 cut(s) 135, 978
BsrI ACTGG 2 cut(s) 538, 791
BssAI RCCGGY 3 cut(s) 429, 802, 806
BssECI CCNNGG 3 cut(s) 219, 280, 827
BssMI GATC 5 cut(s) 37, 105, 165, 196, 618
BssNI GRCGYC 1 cut(s) 437
BssSI CACGAG 1 cut(s) 853
BssT1I CCWWGG 2 cut(s) 219, 827
Bst2BI CACGAG 1 cut(s) 853
Bst4CI ACNGT 2 cut(s) 73, 973
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 437
BstAUI TGTACA 2 cut(s) 135, 978
BstC8I GCNNGC 7 cut(s) 24, 346, 390, 804, 955, 996, 1026
BstDEI CTNAG 2 cut(s) 34, 476
BstDSI CCRYGG 3 cut(s) 219, 280, 827
BstF5I GGATG 3 cut(s) 215, 291, 823
BstFNI CGCG 2 cut(s) 819, 998
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 344, 439
BstKTI GATC 5 cut(s) 40, 108, 168, 199, 621
BstMAI GTCTC 2 cut(s) 151, 962
BstMBI GATC 5 cut(s) 37, 105, 165, 196, 618
BstMCI CGRYCG 1 cut(s) 807
BstMWI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
BstNSI RCATGY 1 cut(s) 152
BstSLI GKGCMC 1 cut(s) 996
BstUI CGCG 2 cut(s) 819, 998
BstV1I GCAGC 3 cut(s) 754, 782, 925
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 1 cut(s) 804
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 4 cut(s) 194, 395, 806, 1067
BtgI CCRYGG 3 cut(s) 219, 280, 827
BtrI CACGTC 1 cut(s) 749
BtsCI GGATG 3 cut(s) 215, 291, 823
BtsI GCAGTG 3 cut(s) 180, 560, 797
BtsIMutI CAGTG 4 cut(s) 180, 545, 560, 797
Cac8I GCNNGC 7 cut(s) 24, 346, 390, 804, 955, 996, 1026
CfoI GCGC 2 cut(s) 344, 439
Cfr10I RCCGGY 3 cut(s) 429, 802, 806
CseI GACGC 1 cut(s) 166
Csp6I GTAC 4 cut(s) 136, 727, 979, 1007
CspAI ACCGGT 1 cut(s) 429
CviAII CATG 8 cut(s) 92, 149, 220, 371, 415, 736, 828, 1043
CviQI GTAC 4 cut(s) 136, 727, 979, 1007
DdeI CTNAG 2 cut(s) 34, 476
DinI GGCGCC 1 cut(s) 438
DpnI GATC 5 cut(s) 39, 107, 167, 198, 620
DpnII GATC 5 cut(s) 37, 105, 165, 196, 618
DraIII CACNNNGTG 1 cut(s) 757
EaeI YGGCCR 3 cut(s) 192, 393, 804
EagI CGGCCG 1 cut(s) 804
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EclXI CGGCCG 1 cut(s) 804
Eco130I CCWWGG 2 cut(s) 219, 827
Eco147I AGGCCT 1 cut(s) 1067
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 151
Eco52I CGGCCG 1 cut(s) 804
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 219, 827
EcoT38I GRGCYC 1 cut(s) 484
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 2 cut(s) 219, 827
FaeI CATG 8 cut(s) 95, 152, 223, 374, 418, 739, 831, 1046
FaqI GGGAC 2 cut(s) 155, 655
FatI CATG 8 cut(s) 91, 148, 219, 370, 414, 735, 827, 1042
FbaI TGATCA 1 cut(s) 618
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
FokI GGATG 3 cut(s) 202, 298, 810
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
FspBI CTAG 4 cut(s) 567, 948, 1010, 1084
GlaI GCGC 2 cut(s) 343, 438
GluI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
GsuI CTGGAG 1 cut(s) 808
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 4 cut(s) 194, 395, 806, 1067
HapII CCGG 5 cut(s) 200, 430, 455, 803, 807
HgaI GACGC 1 cut(s) 166
HhaI GCGC 2 cut(s) 344, 439
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 8 cut(s) 95, 152, 223, 374, 418, 739, 831, 1046
Hin6I GCGC 2 cut(s) 342, 437
HinP1I GCGC 2 cut(s) 342, 437
HincII GTYRAC 2 cut(s) 76, 549
HindII GTYRAC 2 cut(s) 76, 549
HindIII AAGCTT 2 cut(s) 302, 504
HinfI GANTC 2 cut(s) 474, 763
HpaII CCGG 5 cut(s) 200, 430, 455, 803, 807
HphI GGTGA 2 cut(s) 22, 77
Hpy166II GTNNAC 6 cut(s) 76, 136, 243, 515, 549, 979
Hpy188I TCNGA 6 cut(s) 273, 479, 721, 778, 868, 898
Hpy188III TCNNGA 2 cut(s) 200, 567
Hpy8I GTNNAC 6 cut(s) 76, 136, 243, 515, 549, 979
HpyCH4III ACNGT 2 cut(s) 73, 973
HpyCH4IV ACGT 5 cut(s) 249, 259, 517, 748, 963
HpyCH4V TGCA 4 cut(s) 685, 742, 834, 941
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
HpyF3I CTNAG 2 cut(s) 34, 476
HpySE526I ACGT 5 cut(s) 249, 259, 517, 748, 963
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 8 cut(s) 95, 152, 223, 374, 418, 739, 831, 1046
HspAI GCGC 2 cut(s) 342, 437
KasI GGCGCC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 199
KroI GCCGGC 1 cut(s) 802
KroNI GCCGGC 1 cut(s) 804
Ksp22I TGATCA 1 cut(s) 618
Kzo9I GATC 5 cut(s) 37, 105, 165, 196, 618
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 3 cut(s) 754, 782, 925
LweI GCATC 1 cut(s) 1028
MaeI CTAG 4 cut(s) 567, 948, 1010, 1084
MaeII ACGT 5 cut(s) 249, 259, 517, 748, 963
MaeIII GTNAC 4 cut(s) 178, 211, 365, 749
MalI GATC 5 cut(s) 39, 107, 167, 198, 620
MboI GATC 5 cut(s) 37, 105, 165, 196, 618
MboII GAAGA 3 cut(s) 310, 443, 479
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 484, 996
MluCI AATT 6 cut(s) 289, 374, 424, 645, 663, 675
MluI ACGCGT 1 cut(s) 996
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 2 cut(s) 837, 882
MnlI CCTC 6 cut(s) 103, 265, 323, 459, 520, 977
MroI TCCGGA 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 802
MseI TTAA 3 cut(s) 659, 666, 1062
MslI CAYNNNNRTG 2 cut(s) 90, 330
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 5 cut(s) 200, 430, 455, 803, 807
MvnI CGCG 2 cut(s) 819, 998
MwoI GCNNNNNNNGC 7 cut(s) 191, 310, 389, 398, 605, 803, 944
NaeI GCCGGC 1 cut(s) 804
NarI GGCGCC 1 cut(s) 437
NcoI CCATGG 2 cut(s) 219, 827
NdeII GATC 5 cut(s) 37, 105, 165, 196, 618
NgoMIV GCCGGC 1 cut(s) 802
NlaIII CATG 8 cut(s) 95, 152, 223, 374, 418, 739, 831, 1046
NlaIV GGNNCC 5 cut(s) 21, 349, 438, 598, 811
NmuCI GTSAC 3 cut(s) 178, 211, 749
NspI RCATGY 1 cut(s) 152
OliI CACNNNNGTG 1 cut(s) 330
PceI AGGCCT 1 cut(s) 1067
PciI ACATGT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 804
PfeI GAWTC 1 cut(s) 763
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 2 cut(s) 357, 695
PinAI ACCGGT 1 cut(s) 429
PkrI GCNGC 6 cut(s) 187, 394, 460, 744, 772, 940
PleI GAGTC 1 cut(s) 468
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 468
PscI ACATGT 1 cut(s) 148
Psp1406I AACGTT 1 cut(s) 249
PspN4I GGNNCC 5 cut(s) 21, 349, 438, 598, 811
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 4 cut(s) 137, 728, 980, 1008
RsaNI GTAC 4 cut(s) 136, 727, 979, 1007
RseI CAYNNNNRTG 2 cut(s) 90, 330
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 659, 666, 1062
SatI GCNGC 6 cut(s) 186, 393, 459, 743, 771, 939
Sau3AI GATC 5 cut(s) 37, 105, 165, 196, 618
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 1 cut(s) 468
SduI GDGCHC 2 cut(s) 484, 996
SfaNI GCATC 1 cut(s) 1028
SfoI GGCGCC 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 90, 330
Sse9I AATT 6 cut(s) 289, 374, 424, 645, 663, 675
SseBI AGGCCT 1 cut(s) 1067
SsiI CCGC 6 cut(s) 161, 186, 354, 392, 458, 817
SspDI GGCGCC 1 cut(s) 436
SspI AATATT 1 cut(s) 573
SspMI CTAG 4 cut(s) 567, 948, 1010, 1084
StuI AGGCCT 1 cut(s) 1067
StyI CCWWGG 2 cut(s) 219, 827
TaaI ACNGT 2 cut(s) 73, 973
TaiI ACGT 5 cut(s) 252, 262, 520, 751, 966
TaqI TCGA 4 cut(s) 75, 108, 293, 1036
TasI AATT 6 cut(s) 289, 374, 424, 645, 663, 675
TatI WGTACW 3 cut(s) 135, 726, 978
TauI GCSGC 3 cut(s) 188, 395, 461
TfiI GAWTC 1 cut(s) 763
Tru1I TTAA 3 cut(s) 659, 666, 1062
Tru9I TTAA 3 cut(s) 659, 666, 1062
TscAI CASTG 4 cut(s) 187, 545, 560, 804
TseFI GTSAC 3 cut(s) 178, 211, 749
TseI GCWGC 3 cut(s) 742, 770, 938
Tsp45I GTSAC 3 cut(s) 178, 211, 749
TspDTI ATGAA 4 cut(s) 302, 311, 387, 480
TspGWI ACGGA 2 cut(s) 159, 342
TspRI CASTG 4 cut(s) 187, 545, 560, 804
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 2 cut(s) 357, 695
XapI RAATTY 2 cut(s) 289, 374
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 4 cut(s) 567, 948, 1010, 1084
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.