AT1G69240

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012513
Physical Location & Seq
Reverse (-)
26027882 .. 26030312
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69240.1

Sequence Viewer

Length: 1335 bp
ATGGGAAACTCTTTAAGATGCATATCACAAGAACAAGATCCAAACCAGAAGAAACCTAGCTCCGTCGTCAACGGTAATAGCTCCGAGAAACACGTTCGGAGGCTGTCTTTGATACCATCTTTCCGACGCCGGACATTACTACCATCTCTCTCTTGCTCCGGATCCTCAACTTCGTCTACGTCCAAGAAAGGAGGGATCAAGACAAAAAAGAAGATAAGAGAAAGACACCACCAAGAGCAACATCATCATGATCATGAGAAAGACTCTCTTATTCAAGACCAAACATTAGCTGCCACTAATATTCTCTTTAGTCAAACGCCTCGTAACAGCAACTCTGCTCCTCCTTTCCGACGCTCCACCTCCGTCGTTTACACTCAGCCACCTACCGCCGCTGTAGCCGCCTCCGTAGGTTCTGTTTCCGGCGCCTTGACTCCTAAGAAATCCACTTATGGATACGTTAGAAGTTCTAGCAATAGGCAGAGATCAAGCACTGATCCTGTTCTTAAACCCAATCAGCTTCTAGACAAGGAGCTAAAGGTCGAAGGTGCGGAGACAAAGCGGTTCGTGCTGGTTCATGGAGGAGGGTTTGGTGCATGGTGTTGGTACAAAACCATAACGCTCTTAGAGAAACATGGTTTCCAAGTAGACGCCGTTGACTTGACCGGTTCCGGTGTCAGCTCTTTTGACACAAACAACATCACTAGCCTCGCTCAATACGTCAAACCACTCCTCCATTTCTTTGACACCCTTAAACCCACCGAGAAGGTGATATTAGTGGGGCATGATTTTGGAGGAGCTTGTATGTCTTATGCCATGGAGATGTACCCTTCTAAGATCGCTAAGGCTATTTTCATATCTGCTGCTATGTTGGCTAATGCCCAAAGCACTCTCGATCTCTTTAACCAACAGCCTGACTCAAATTATGATCTGATGGAACAAGTCCATCTATTTCTGTACGCCAACGGCAAAAAGAACCCTCCAACAGCCGTTGATTTCGACAGATCATTGCTTAGAGATTTTTTCTTTAATCAGAGCCCTCCAAAGGACGTTGCATTAGCGTCGGTATCGATGAGACCAATCCCGTTCGCACCAGTGGTCGAAAAGCTCCACGTGTCGGAGAAAAATTACGGTTCAATCCGACGGTTCTACATCAAAACCATGGAGGATGATTACGCTGTACCGGTTTCTCTCCAAGATGCAATGATCAAATCAAACCCTCCAGAACAAGTTTTTCATCTCAAAGGCTCTGACCATGCACCTTTCTTCTCTCGTCCTCAGTCTTTGAACCGGATACTCGTTGAGATTTCTCAATTACCTCCCAAGAAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

444

Amino Acids

49.33

Weight (kDa)

9.55

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 186 - 295 1.5e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 187 - 313 2.1e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 188 - 428 3.7e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 422
AccI GTMKAC 2 cut(s) 176, 645
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 5 cut(s) 387, 390, 399, 548, 559
AclWI GGATC 5 cut(s) 32, 156, 169, 203, 488
AcvI CACGTG 1 cut(s) 1111
AcyI GRCGYC 3 cut(s) 127, 423, 648
AfaI GTAC 4 cut(s) 605, 824, 956, 1179
AfiI CCNNNNNNNGG 2 cut(s) 1042, 1114
AflIII ACRYGT 2 cut(s) 91, 1110
AgeI ACCGGT 2 cut(s) 662, 1180
AgsI TTSAA 3 cut(s) 275, 1134, 1285
AluBI AGCT 8 cut(s) 60, 81, 290, 517, 532, 678, 797, 1105
AluI AGCT 8 cut(s) 60, 81, 290, 517, 532, 678, 797, 1105
Alw26I GTCTC 2 cut(s) 545, 1066
AlwI GGATC 5 cut(s) 32, 156, 169, 203, 488
Aor13HI TCCGGA 1 cut(s) 158
ApeKI GCWGC 2 cut(s) 290, 860
AsiGI ACCGGT 2 cut(s) 662, 1180
AspLEI GCGC 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 778
BamHI GGATCC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 1037
BbrPI CACGTG 1 cut(s) 1111
BbvI GCAGC 2 cut(s) 277, 847
BccI CCATC 4 cut(s) 124, 151, 925, 951
BceAI ACGGC 3 cut(s) 635, 971, 979
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciVI GTATCC 2 cut(s) 446, 1284
BclI TGATCA 2 cut(s) 250, 1203
BcoDI GTCTC 2 cut(s) 545, 1066
BfaI CTAG 4 cut(s) 57, 468, 521, 702
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 426
BfuI GTATCC 2 cut(s) 446, 1284
BisI GCNGC 4 cut(s) 291, 390, 399, 861
BlsI GCNGC 4 cut(s) 292, 391, 400, 862
BmiI GGNNCC 3 cut(s) 163, 424, 667
BmsI GCATC 2 cut(s) 8, 1186
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 840
Bsa29I ATCGAT 1 cut(s) 1067
BsaAI YACGTR 1 cut(s) 1111
BsaBI GATNNNNATC 1 cut(s) 22
BsaHI GRCGYC 3 cut(s) 127, 423, 648
BsaI GGTCTC 1 cut(s) 1066
BsaJI CCNNGG 2 cut(s) 813, 1158
BsaWI WCCGGW 5 cut(s) 158, 662, 668, 1180, 1287
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 1042, 1114
Bse118I RCCGGY 2 cut(s) 662, 1180
Bse1I ACTGG 1 cut(s) 1091
Bse3DI GCAATG 2 cut(s) 1004, 1206
Bse8I GATNNNNATC 1 cut(s) 22
BseAI TCCGGA 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 1067
BseDI CCNNGG 2 cut(s) 813, 1158
BseGI GGATG 1 cut(s) 1171
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 1042, 1114
BseMI GCAATG 2 cut(s) 1004, 1206
BseMII CTCAG 2 cut(s) 389, 1289
BseNI ACTGG 1 cut(s) 1091
BseRI GAGGAG 4 cut(s) 330, 594, 719, 807
BseXI GCAGC 2 cut(s) 277, 847
BshNI GGYRCC 1 cut(s) 422
BshTI ACCGGT 2 cut(s) 662, 1180
BshVI ATCGAT 1 cut(s) 1067
BsiSI CCGG 7 cut(s) 130, 159, 420, 663, 669, 1181, 1288
BslI CCNNNNNNNGG 2 cut(s) 1042, 1114
BsmAI GTCTC 2 cut(s) 545, 1066
Bso31I GGTCTC 1 cut(s) 1066
Bsp1286I GDGCHC 1 cut(s) 1037
Bsp13I TCCGGA 1 cut(s) 158
Bsp19I CCATGG 2 cut(s) 813, 1158
BspACI CCGC 5 cut(s) 387, 390, 399, 548, 559
BspCNI CTCAG 2 cut(s) 388, 1288
BspDI ATCGAT 1 cut(s) 1067
BspEI TCCGGA 1 cut(s) 158
BspHI TCATGA 2 cut(s) 247, 253
BspLI GGNNCC 3 cut(s) 163, 424, 667
BspPI GGATC 5 cut(s) 32, 156, 169, 203, 488
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 1066
BsrDI GCAATG 2 cut(s) 1004, 1206
BsrFI RCCGGY 2 cut(s) 662, 1180
BsrI ACTGG 1 cut(s) 1091
BssAI RCCGGY 2 cut(s) 662, 1180
BssECI CCNNGG 2 cut(s) 813, 1158
BssNI GRCGYC 3 cut(s) 127, 423, 648
BssT1I CCWWGG 2 cut(s) 813, 1158
Bst4CI ACNGT 3 cut(s) 74, 1130, 1143
BstACI GRCGYC 3 cut(s) 127, 423, 648
BstBAI YACGTR 1 cut(s) 1111
BstDEI CTNAG 7 cut(s) 375, 435, 622, 831, 840, 1010, 1275
BstDSI CCRYGG 2 cut(s) 813, 1158
BstF5I GGATG 1 cut(s) 1171
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstMAI GTCTC 2 cut(s) 545, 1066
BstMWI GCNNNNNNNGC 4 cut(s) 395, 398, 565, 869
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 277, 847
BstX2I RGATCY 2 cut(s) 37, 161
BstYI RGATCY 2 cut(s) 37, 161
Bsu15I ATCGAT 1 cut(s) 1067
BsuI GTATCC 2 cut(s) 446, 1284
BsuTUI ATCGAT 1 cut(s) 1067
BtgI CCRYGG 2 cut(s) 813, 1158
BtsCI GGATG 1 cut(s) 1171
BtsIMutI CAGTG 2 cut(s) 489, 1098
CciI TCATGA 2 cut(s) 247, 253
CfoI GCGC 1 cut(s) 425
Cfr10I RCCGGY 2 cut(s) 662, 1180
ClaI ATCGAT 1 cut(s) 1067
CseI GACGC 4 cut(s) 135, 360, 656, 1047
Csp6I GTAC 4 cut(s) 604, 823, 955, 1178
CspAI ACCGGT 2 cut(s) 662, 1180
CviAII CATG 9 cut(s) 248, 254, 575, 594, 632, 782, 814, 1159, 1253
CviQI GTAC 4 cut(s) 604, 823, 955, 1178
DdeI CTNAG 7 cut(s) 375, 435, 622, 831, 840, 1010, 1275
DinI GGCGCC 1 cut(s) 424
Eco130I CCWWGG 2 cut(s) 813, 1158
Eco24I GRGCYC 1 cut(s) 1037
Eco31I GGTCTC 1 cut(s) 1066
Eco72I CACGTG 1 cut(s) 1111
EcoT14I CCWWGG 2 cut(s) 813, 1158
EcoT22I ATGCAT 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 1037
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
ErhI CCWWGG 2 cut(s) 813, 1158
FaeI CATG 9 cut(s) 251, 257, 578, 597, 635, 785, 817, 1162, 1256
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FatI CATG 9 cut(s) 247, 253, 574, 593, 631, 781, 813, 1158, 1252
FbaI TGATCA 2 cut(s) 250, 1203
FblI GTMKAC 2 cut(s) 176, 645
Fnu4HI GCNGC 4 cut(s) 291, 390, 399, 861
FokI GGATG 1 cut(s) 1178
FriOI GRGCYC 1 cut(s) 1037
Fsp4HI GCNGC 4 cut(s) 291, 390, 399, 861
FspBI CTAG 4 cut(s) 57, 468, 521, 702
GlaI GCGC 1 cut(s) 424
GluI GCNGC 4 cut(s) 291, 390, 399, 861
GsuI CTGGAG 1 cut(s) 1203
HaeII RGCGCY 1 cut(s) 426
HapII CCGG 7 cut(s) 130, 159, 420, 663, 669, 1181, 1288
HgaI GACGC 4 cut(s) 135, 360, 656, 1047
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 3 cut(s) 127, 423, 648
Hin1II CATG 9 cut(s) 251, 257, 578, 597, 635, 785, 817, 1162, 1256
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 2 cut(s) 70, 655
HindII GTYRAC 2 cut(s) 70, 655
HinfI GANTC 3 cut(s) 263, 430, 914
HpaII CCGG 7 cut(s) 130, 159, 420, 663, 669, 1181, 1288
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 5 cut(s) 70, 177, 370, 646, 655
Hpy188I TCNGA 9 cut(s) 85, 99, 125, 350, 930, 1032, 1117, 1139, 1249
Hpy188III TCNNGA 9 cut(s) 159, 199, 248, 254, 275, 521, 890, 1220, 1332
Hpy8I GTNNAC 5 cut(s) 70, 177, 370, 646, 655
Hpy99I CGWCG 6 cut(s) 68, 129, 354, 368, 1063, 1143
HpyAV CCTTC 3 cut(s) 536, 757, 837
HpyCH4III ACNGT 3 cut(s) 74, 1130, 1143
HpyCH4IV ACGT 6 cut(s) 93, 179, 456, 717, 1047, 1110
HpyCH4V TGCA 5 cut(s) 21, 593, 1052, 1199, 1256
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 398, 565, 869
HpyF3I CTNAG 7 cut(s) 375, 435, 622, 831, 840, 1010, 1275
HpySE526I ACGT 6 cut(s) 93, 179, 456, 717, 1047, 1110
Hsp92I GRCGYC 3 cut(s) 127, 423, 648
Hsp92II CATG 9 cut(s) 251, 257, 578, 597, 635, 785, 817, 1162, 1256
HspAI GCGC 1 cut(s) 423
KasI GGCGCC 1 cut(s) 422
Kpn2I TCCGGA 1 cut(s) 158
Ksp22I TGATCA 2 cut(s) 250, 1203
LmnI GCTCC 8 cut(s) 65, 86, 161, 343, 359, 529, 794, 1110
Lsp1109I GCAGC 2 cut(s) 277, 847
LweI GCATC 2 cut(s) 8, 1186
MaeI CTAG 4 cut(s) 57, 468, 521, 702
MaeII ACGT 6 cut(s) 93, 179, 456, 717, 1047, 1110
MaeIII GTNAC 1 cut(s) 323
MboII GAAGA 4 cut(s) 61, 223, 1255, 1320
MflI RGATCY 2 cut(s) 37, 161
MhlI GDGCHC 1 cut(s) 1037
MluCI AATT 3 cut(s) 919, 1123, 1310
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 3 cut(s) 257, 424, 908
MmeI TCCRAC 5 cut(s) 148, 373, 1004, 1095, 1162
Mph1103I ATGCAT 1 cut(s) 23
MroI TCCGGA 1 cut(s) 158
MseI TTAA 5 cut(s) 14, 504, 750, 900, 1026
MslI CAYNNNNRTG 3 cut(s) 246, 252, 818
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 7 cut(s) 130, 159, 420, 663, 669, 1181, 1288
MwoI GCNNNNNNNGC 4 cut(s) 395, 398, 565, 869
NarI GGCGCC 1 cut(s) 423
NcoI CCATGG 2 cut(s) 813, 1158
NlaIII CATG 9 cut(s) 251, 257, 578, 597, 635, 785, 817, 1162, 1256
NlaIV GGNNCC 3 cut(s) 163, 424, 667
NsiI ATGCAT 1 cut(s) 23
PagI TCATGA 2 cut(s) 247, 253
PcsI WCGNNNNNNNCGW 1 cut(s) 714
PinAI ACCGGT 2 cut(s) 662, 1180
PkrI GCNGC 4 cut(s) 292, 391, 400, 862
PleI GAGTC 3 cut(s) 257, 424, 908
PluTI GGCGCC 1 cut(s) 426
PmaCI CACGTG 1 cut(s) 1111
PmlI CACGTG 1 cut(s) 1111
PpsI GAGTC 3 cut(s) 257, 424, 908
Ppu21I YACGTR 1 cut(s) 1111
PspCI CACGTG 1 cut(s) 1111
PspN4I GGNNCC 3 cut(s) 163, 424, 667
PsuI RGATCY 2 cut(s) 37, 161
RsaI GTAC 4 cut(s) 605, 824, 956, 1179
RsaNI GTAC 4 cut(s) 604, 823, 955, 1178
RseI CAYNNNNRTG 3 cut(s) 246, 252, 818
SaqAI TTAA 5 cut(s) 14, 504, 750, 900, 1026
SatI GCNGC 4 cut(s) 291, 390, 399, 861
SchI GAGTC 3 cut(s) 257, 424, 908
SduI GDGCHC 1 cut(s) 1037
SfaNI GCATC 2 cut(s) 8, 1186
SfcI CTRYAG 1 cut(s) 393
SfoI GGCGCC 1 cut(s) 424
SmiMI CAYNNNNRTG 3 cut(s) 246, 252, 818
Sse9I AATT 3 cut(s) 919, 1123, 1310
SsiI CCGC 5 cut(s) 387, 390, 399, 548, 559
SspDI GGCGCC 1 cut(s) 422
SspI AATATT 1 cut(s) 301
SspMI CTAG 4 cut(s) 57, 468, 521, 702
StyI CCWWGG 2 cut(s) 813, 1158
TaaI ACNGT 3 cut(s) 74, 1130, 1143
TaiI ACGT 6 cut(s) 96, 182, 459, 720, 1050, 1113
TaqI TCGA 5 cut(s) 540, 891, 996, 1067, 1098
TasI AATT 3 cut(s) 919, 1123, 1310
TauI GCSGC 2 cut(s) 392, 401
Tru1I TTAA 5 cut(s) 14, 504, 750, 900, 1026
Tru9I TTAA 5 cut(s) 14, 504, 750, 900, 1026
TscAI CASTG 2 cut(s) 496, 1098
TseI GCWGC 2 cut(s) 290, 860
TspDTI ATGAA 3 cut(s) 563, 841, 1223
TspGWI ACGGA 3 cut(s) 52, 352, 394
TspRI CASTG 2 cut(s) 496, 1098
XbaI TCTAGA 1 cut(s) 520
XmiI GTMKAC 2 cut(s) 176, 645
XspI CTAG 4 cut(s) 57, 468, 521, 702
Zsp2I ATGCAT 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.