Rorug04G0307000

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012513
Physical Location & Seq
Reverse (-)
48304227 .. 48305962
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0307000.1

Sequence Viewer

Length: 948 bp
ATGGCTATCCTTAACTCGGACCAAGATGGCGTTTTCAGATCACTTTCAGATTCACTCCCAACTCATTGCACTCTAAATATAGAGTCGTTTTCGTTGCTAGCTGAATATCATGGAGTCAGTGGATATGAATCAAAAGAGTTCGAAGCCGGAGGATACATATGGAAACTGGTGCTGTACCCAAATGGAAACAAGAAGAAGAATGTGGAAGACCACATCTCTGTCTACTTGCGCATAGCCGGAGAAGATTCACTTCAGACTGGTTGGGGAGTATCTGTTGATTTTAGATTGTTTTTGCTTGATCAGAATAAGGGAATGTACTTGGTTCTTGAAGATGCTTTTACAAAGCAGAAGTGCTTTCACGCAGGGTTGCTTCATGTAGGTTTTGATAAGCTAATCCGTCTTAAAGAATTTACTGATGTTTCCAATGGATATCTCATCGATGACAAGTGTGTATTTGGAGCTGAGGTCTTTGTTTGCAAAGAAAGAAAAACTGGCCAGGCAGAGTGTGTATCAAGGATAAAGAGCCCTTGTATGTACAAGCATGTTTGGAAACTTGAAAAATTTTCAAAGTTGAACGCTCAGTTCTACCACTCAAAACAATTCAGTGCTGAAGGACTAAACTGGGCTATAGTAATCTATCCCAAGAGGAAAAGCACCACAAACGATCCTTCTCATCTGTCTGTTTACTTGCAATGGGTTGCTGAAAAAACAAGTCATGTCACCAAAGTATTTACAGAGTATGCCTTACGCATTGTAGATCGAAATCATGCCAGCATTCAATATCGCTCAGATAAGTACTGTTTTAACACTGCCTCTCGGACTCATGGTTGGCCTGAATTCATTTCACTTGGTGATTTAAGTCGTCTTTTGAAGTACGATACTTGCCTAGTGGAGGCAGAGGTCACTGTTCGTGCAATTGCTAAGATTCTTGCTAAAGATGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

315

Amino Acids

35.96

Weight (kDa)

8.25

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 22 - 156 6.7e-31 MATH domain
MATH PF00917 40 - 158 8.9e-12 MATH domain
MATH_2 PF22486 180 - 301 4.3e-22 MATH domain
MATH PF00917 187 - 303 2.1e-13 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 230
AccI GTMKAC 1 cut(s) 222
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 3 cut(s) 407, 560, 836
AcuI CTGAAG 2 cut(s) 236, 630
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 5 cut(s) 176, 317, 536, 797, 875
AfiI CCNNNNNNNGG 2 cut(s) 16, 892
AgsI TTSAA 6 cut(s) 329, 557, 567, 574, 779, 871
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 3 cut(s) 101, 391, 461
AluI AGCT 3 cut(s) 101, 391, 461
AlwI GGATC 1 cut(s) 659
AoxI GGCC 2 cut(s) 493, 830
ApoI RAATTY 3 cut(s) 407, 560, 836
AspLEI GCGC 1 cut(s) 231
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 712, 863
AsuII TTCGAA 1 cut(s) 141
AsuNHI GCTAGC 1 cut(s) 97
AvaII GGWCC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 495
BanII GRGCYC 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 462
BccI CCATC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 146
BclI TGATCA 1 cut(s) 298
BfaI CTAG 2 cut(s) 98, 887
BfmI CTRYAG 1 cut(s) 627
BfuI GTATCC 1 cut(s) 146
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 1 cut(s) 497
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 497
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 2 cut(s) 322, 928
BmtI GCTAGC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 631
BpiI GAAGAC 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 438
BsaBI GATNNNNATC 2 cut(s) 127, 762
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 450, 480
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 892
Bse1I ACTGG 4 cut(s) 171, 262, 496, 626
Bse3DI GCAATG 2 cut(s) 64, 698
Bse8I GATNNNNATC 2 cut(s) 127, 762
BseBI CCWGG 1 cut(s) 497
BseCI ATCGAT 1 cut(s) 438
BseJI GATNNNNATC 2 cut(s) 127, 762
BseLI CCNNNNNNNGG 2 cut(s) 16, 892
BseMI GCAATG 2 cut(s) 64, 698
BseMII CTCAG 3 cut(s) 453, 593, 801
BseNI ACTGG 4 cut(s) 171, 262, 496, 626
BshFI GGCC 2 cut(s) 495, 832
BshVI ATCGAT 1 cut(s) 438
BsiSI CCGG 2 cut(s) 147, 237
BslI CCNNNNNNNGG 2 cut(s) 16, 892
BsmI GAATGC 1 cut(s) 774
BsnI GGCC 2 cut(s) 495, 832
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 527
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 4 cut(s) 38, 298, 664, 757
BspANI GGCC 2 cut(s) 495, 832
BspCNI CTCAG 3 cut(s) 454, 592, 800
BspDI ATCGAT 1 cut(s) 438
BspOI GCTAGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 141
BsrDI GCAATG 2 cut(s) 64, 698
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 4 cut(s) 171, 262, 496, 626
BssMI GATC 4 cut(s) 38, 298, 664, 757
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 2 cut(s) 800, 907
BstAUI TGTACA 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 3 cut(s) 99, 772, 943
BstDEI CTNAG 4 cut(s) 462, 579, 787, 921
BstENI CCTNNNNNAGG 1 cut(s) 890
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 4 cut(s) 41, 301, 667, 760
BstMBI GATC 4 cut(s) 38, 298, 664, 757
BstMWI GCNNNNNNNGC 1 cut(s) 938
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 2 cut(s) 545, 945
BstSCI CCNGG 1 cut(s) 495
BstSFI CTRYAG 1 cut(s) 627
BstV2I GAAGAC 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 2 cut(s) 495, 832
BsuTUI ATCGAT 1 cut(s) 438
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 4 cut(s) 124, 610, 807, 903
Cac8I GCNNGC 3 cut(s) 99, 772, 943
CfoI GCGC 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 19
ClaI ATCGAT 1 cut(s) 438
Csp6I GTAC 5 cut(s) 175, 316, 535, 796, 874
CviAII CATG 7 cut(s) 110, 374, 542, 716, 767, 824, 942
CviQI GTAC 5 cut(s) 175, 316, 535, 796, 874
DdeI CTNAG 4 cut(s) 462, 579, 787, 921
DpnI GATC 4 cut(s) 40, 300, 666, 759
DpnII GATC 4 cut(s) 38, 298, 664, 757
DraIII CACNNNGTG 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 236, 630
EcoNI CCTNNNNNAGG 1 cut(s) 890
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 943
EcoT38I GRGCYC 1 cut(s) 527
FaeI CATG 7 cut(s) 113, 377, 545, 719, 770, 827, 945
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 7 cut(s) 109, 373, 541, 715, 766, 823, 941
FauNDI CATATG 1 cut(s) 158
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 2 cut(s) 98, 887
FspI TGCGCA 1 cut(s) 230
GlaI GCGC 1 cut(s) 230
HaeIII GGCC 2 cut(s) 495, 832
HapII CCGG 2 cut(s) 147, 237
HhaI GCGC 1 cut(s) 231
Hin1II CATG 7 cut(s) 113, 377, 545, 719, 770, 827, 945
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HinfI GANTC 7 cut(s) 50, 83, 114, 128, 245, 820, 925
HpaII CCGG 2 cut(s) 147, 237
HphI GGTGA 2 cut(s) 712, 863
Hpy166II GTNNAC 2 cut(s) 223, 685
Hpy188I TCNGA 7 cut(s) 19, 38, 49, 255, 303, 790, 819
Hpy188III TCNNGA 1 cut(s) 326
Hpy8I GTNNAC 2 cut(s) 223, 685
HpyAV CCTTC 2 cut(s) 605, 678
HpyCH4III ACNGT 2 cut(s) 800, 907
HpyCH4V TGCA 5 cut(s) 69, 477, 691, 914, 941
HpyF10VI GCNNNNNNNGC 1 cut(s) 938
HpyF3I CTNAG 4 cut(s) 462, 579, 787, 921
Hsp92II CATG 7 cut(s) 113, 377, 545, 719, 770, 827, 945
HspAI GCGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 38, 298, 664, 757
LmnI GCTCC 1 cut(s) 458
LweI GCATC 2 cut(s) 322, 928
MaeI CTAG 2 cut(s) 98, 887
MaeIII GTNAC 2 cut(s) 718, 901
MalI GATC 4 cut(s) 40, 300, 666, 759
MboI GATC 4 cut(s) 38, 298, 664, 757
MboII GAAGA 5 cut(s) 205, 208, 218, 254, 341
MfeI CAATTG 1 cut(s) 915
MhlI GDGCHC 1 cut(s) 527
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 5 cut(s) 407, 560, 599, 836, 915
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 3 cut(s) 92, 123, 814
MnlI CCTC 6 cut(s) 143, 457, 639, 823, 886, 892
Mox20I TGGCCA 1 cut(s) 495
Mph1103I ATGCAT 1 cut(s) 943
MscI TGGCCA 1 cut(s) 495
MseI TTAA 4 cut(s) 12, 402, 804, 857
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 2 cut(s) 147, 237
MspR9I CCNGG 1 cut(s) 497
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 1 cut(s) 938
NdeI CATATG 1 cut(s) 158
NdeII GATC 4 cut(s) 38, 298, 664, 757
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 7 cut(s) 113, 377, 545, 719, 770, 827, 945
NmuCI GTSAC 2 cut(s) 718, 901
NsbI TGCGCA 1 cut(s) 230
NsiI ATGCAT 1 cut(s) 943
NspI RCATGY 2 cut(s) 545, 945
NspV TTCGAA 1 cut(s) 141
PaeI GCATGC 1 cut(s) 945
PctI GAATGC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 50, 128, 245, 925
PleI GAGTC 3 cut(s) 91, 122, 814
PpsI GAGTC 3 cut(s) 91, 122, 814
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspPI GGNCC 1 cut(s) 19
RsaI GTAC 5 cut(s) 176, 317, 536, 797, 875
RsaNI GTAC 5 cut(s) 175, 316, 535, 796, 874
SaqAI TTAA 4 cut(s) 12, 402, 804, 857
Sau3AI GATC 4 cut(s) 38, 298, 664, 757
Sau96I GGNCC 1 cut(s) 19
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 3 cut(s) 92, 123, 814
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 1 cut(s) 527
SetI ASST 6 cut(s) 103, 382, 393, 463, 468, 903
SfaNI GCATC 2 cut(s) 322, 928
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 19
SphI GCATGC 1 cut(s) 945
Sse9I AATT 5 cut(s) 407, 560, 599, 836, 915
SspMI CTAG 2 cut(s) 98, 887
StyD4I CCNGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 800, 907
TaqI TCGA 3 cut(s) 141, 438, 760
TasI AATT 5 cut(s) 407, 560, 599, 836, 915
TatI WGTACW 3 cut(s) 315, 534, 795
TfiI GAWTC 4 cut(s) 50, 128, 245, 925
Tru1I TTAA 4 cut(s) 12, 402, 804, 857
Tru9I TTAA 4 cut(s) 12, 402, 804, 857
TscAI CASTG 4 cut(s) 124, 610, 814, 910
TseFI GTSAC 2 cut(s) 718, 901
Tsp45I GTSAC 2 cut(s) 718, 901
TspDTI ATGAA 3 cut(s) 141, 362, 829
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 4 cut(s) 124, 610, 814, 910
VpaK11BI GGWCC 1 cut(s) 19
XagI CCTNNNNNAGG 1 cut(s) 890
XapI RAATTY 3 cut(s) 407, 560, 836
XceI RCATGY 2 cut(s) 545, 945
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 98, 887
ZrmI AGTACT 1 cut(s) 797
Zsp2I ATGCAT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.