RLG00000006388

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012513
Physical Location & Seq
Forward (+)
6689783 .. 6697229
7447 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006388

Sequence Viewer

Length: 1638 bp
ATGGGAAACTCAATGGCATGCTTCTCCCCTGCGCACATACCAAAGAAGCTGAGCACCAACTTGTCAAGGAAGCCCTCCAAGAGGTCACCCAACTCTTCACCTTTCTTTGGCTCCTCGGCTAGTACTAGAAGAACCAAGGGTTCTCCCAAGAAGACAGAGACCCTTGCTGATGATTTGATGCTTCAACAACAAGCTATAGAGGCGGTTCTGTTGTTCCAGAATCACCAGAAGAACAATGGATCGCAACCCTTCAACCGGTCCACCTCTGTGGTGTTTCCGGCTCCTAGACCAAAGAAGAATCAGGCCTTCACGAAAAGCTCCAGCTCTAGGCAGCCTTTGGGTTCTGAGACTCTCTTTCAGCCTCTTGAGCTTGTTGACAAGCAGGATGTAAAGATTGATGGTCTGGAAACAAATCATGTTGTGCTTGTTCACGGAGGTGGCTTTGGTGCTTGGTGCTGGTATAAAACAATGACACTTCTAGAAGAAAGTGGGTTCAAGGTTGATGCAGTTGACTTAACTGGTTCCGGAATCCACTCATTTGATACAAACAGCATTACAAGTCTTGCACAATATGTGAAGCCACTTACTGAAATCCTTGAGAATCTAGAGGAGGGGAAAAAGGTCATTTTGGTTGGACATGATTTTGGTGGTACATGTATCTCATATGCAATGGAGTTGTTTCCATCTAAAATTGCAAAGGCCATCTTCATATCTGCAGCAATGTTAACTAGTGGACAGAGTACTCTTAATCTGTTTGCTCAACAGACAGGCTCGAACGATCTAATGCGTCAGGCTCAGATATTCTTGTATGCAAATGGGAAGGATCAGCCTCCTACTGCTATTGGTCTCGGCAAAAAATTTACCGAAGACTTGTTGTTCAATCAAAGTCCAGCAAAGGATGTTGCATTGGCATCAGTATCAATGAGGCCAATCCCTTTTGCACCAGTAACTGAGAAGCTCTCTCTTTCTGACAAGAATTACGGATCTGTCAGGCGGTTTTTCATAGTCACTCAAGAAGATCATGCCATCAGCGTTCCACTACAGGAGGCAATGCTAGAATCAAACCCGCCAGAACAATTTTATCGACTAAAAGGCTCTGATCATGCACCTTTTTTCTCAAGGCCTCAGGCTTTGCATAGGATATTAGTAGAGATATCCCAGCTTTTTAGCTGTCTCAGTCGCTCAACCTTACACTCTCTCTCTCTCTCTCTGCTCCTCCCTCCTTCCTCTTCACAAATCTCATCTCAACCACCGCCAGAGTTATCGTTGAAGAATATGGGTCGTGGAGTCAGCAGTGGTGGGGGTCAGAGTTCCTTGGACTATCTTTTTGGGAGTGGAGAGGCTCCAAAGCCAGGCACAAACAATGTCCAAGCTCCACCAAGGGAAGTGCAGACTGTGAGTGAACCTACTCCCAAACCTACTGCTTCTCCTCCTCCCCCCAAACCTGAATCAGTAGATATCACCAAGCAGATTCCAGCTGGTATCCATAGCACTTCTTCAAATAACTACCTGCGTGCGGATGGCCAAAACACTGGAAACTTCATTACGGATCGGCCCTCAACTAAGGTCCACTCTGCCCCAGGTGGTGGATCTTCTCTGGGTTACCTCTTTGGTGGACCTGGACCAGCTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

546

Amino Acids

58.74

Weight (kDa)

9.02

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 138 - 305 4.1e-15 alpha/beta hydrolase fold
Hydrolase_4 PF12146 139 - 250 3.6e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 140 - 378 9.8e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 1518
AccB7I CCANNNNNTGG 1 cut(s) 1586
AccIII TCCGGA 1 cut(s) 524
AciI CCGC 5 cut(s) 203, 994, 1067, 1253, 1517
AclWI GGATC 5 cut(s) 247, 831, 991, 1557, 1597
AcoI YGGCCR 1 cut(s) 1522
AcsI RAATTY 1 cut(s) 857
AfaI GTAC 3 cut(s) 124, 652, 742
AfiI CCNNNNNNNGG 7 cut(s) 81, 107, 255, 327, 1352, 1516, 1586
AflIII ACRYGT 1 cut(s) 653
AgeI ACCGGT 1 cut(s) 255
AgsI TTSAA 6 cut(s) 185, 253, 496, 880, 1270, 1500
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 3 cut(s) 1351, 1579, 1618
AleI CACNNNNGTG 2 cut(s) 266, 435
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 4 cut(s) 152, 341, 851, 1178
AlwI GGATC 5 cut(s) 247, 831, 991, 1557, 1597
AlwNI CAGNNNCTG 1 cut(s) 950
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 6 cut(s) 303, 699, 926, 1121, 1522, 1553
ApeKI GCWGC 2 cut(s) 331, 716
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 2 cut(s) 1310, 1342
AsiGI ACCGGT 1 cut(s) 255
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 5 cut(s) 258, 1554, 1567, 1616, 1622
AsuHPI GGTGA 4 cut(s) 78, 90, 215, 1453
AvaII GGWCC 4 cut(s) 258, 1567, 1616, 1622
AxyI CCTNAGG 1 cut(s) 1125
BalI TGGCCA 1 cut(s) 1524
BbsI GAAGAC 2 cut(s) 158, 873
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 343, 728
BccI CCATC 5 cut(s) 392, 691, 710, 1034, 1514
BciT130I CCWGG 3 cut(s) 1353, 1581, 1620
BciVI GTATCC 1 cut(s) 1493
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 4 cut(s) 152, 341, 851, 1178
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 8 cut(s) 120, 126, 285, 327, 479, 605, 729, 1055
BfmI CTRYAG 3 cut(s) 195, 714, 1040
BfuAI ACCTGC 1 cut(s) 1518
BfuI GTATCC 1 cut(s) 1493
BisI GCNGC 2 cut(s) 332, 717
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 2 cut(s) 333, 718
BmcAI AGTACT 2 cut(s) 124, 742
Bme1390I CCNGG 3 cut(s) 1353, 1581, 1620
Bme18I GGWCC 4 cut(s) 258, 1567, 1616, 1622
BmgT120I GGNCC 5 cut(s) 258, 1554, 1567, 1616, 1622
BmiI GGNNCC 4 cut(s) 112, 282, 523, 1344
BmrFI CCNGG 3 cut(s) 1353, 1581, 1620
BmsI GCATC 3 cut(s) 168, 493, 920
BpiI GAAGAC 2 cut(s) 158, 873
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpmI CTGGAG 1 cut(s) 304
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 4 cut(s) 386, 617, 996, 1102
BsaI GGTCTC 2 cut(s) 152, 851
BsaJI CCNNGG 5 cut(s) 114, 135, 1314, 1379, 1579
BsaWI WCCGGW 2 cut(s) 255, 524
BsaXI ACNNNNNCTCC 2 cut(s) 1411, 1441
Bsc4I CCNNNNNNNGG 7 cut(s) 81, 107, 255, 327, 1352, 1516, 1586
Bse118I RCCGGY 1 cut(s) 255
Bse1I ACTGG 3 cut(s) 523, 944, 1537
Bse21I CCTNAGG 1 cut(s) 1125
Bse3DI GCAATG 3 cut(s) 675, 726, 1056
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 3 cut(s) 1353, 1581, 1620
BseDI CCNNGG 5 cut(s) 114, 135, 1314, 1379, 1579
BseGI GGATG 3 cut(s) 391, 904, 1525
BseLI CCNNNNNNNGG 7 cut(s) 81, 107, 255, 327, 1352, 1516, 1586
BseMI GCAATG 3 cut(s) 675, 726, 1056
BseMII CTCAG 6 cut(s) 41, 336, 809, 942, 1139, 1189
BseNI ACTGG 3 cut(s) 523, 944, 1537
BseRI GAGGAG 5 cut(s) 103, 623, 1205, 1419, 1422
BseXI GCAGC 2 cut(s) 343, 728
BseYI CCCAGC 1 cut(s) 1158
BsgI GTGCAG 1 cut(s) 1409
BshFI GGCC 6 cut(s) 305, 701, 928, 1123, 1524, 1555
BshTI ACCGGT 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 256, 278, 525
BslI CCNNNNNNNGG 7 cut(s) 81, 107, 255, 327, 1352, 1516, 1586
BsmAI GTCTC 4 cut(s) 152, 341, 851, 1178
BsnI GGCC 6 cut(s) 305, 701, 928, 1123, 1524, 1555
Bso31I GGTCTC 2 cut(s) 152, 851
Bsp1286I GDGCHC 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 5 cut(s) 203, 994, 1067, 1253, 1517
BspANI GGCC 6 cut(s) 305, 701, 928, 1123, 1524, 1555
BspCNI CTCAG 6 cut(s) 42, 337, 808, 943, 1138, 1188
BspEI TCCGGA 1 cut(s) 524
BspLI GGNNCC 4 cut(s) 112, 282, 523, 1344
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 1518
BspPI GGATC 5 cut(s) 247, 831, 991, 1557, 1597
BspTNI GGTCTC 2 cut(s) 152, 851
BsrDI GCAATG 3 cut(s) 675, 726, 1056
BsrFI RCCGGY 1 cut(s) 255
BsrI ACTGG 3 cut(s) 523, 944, 1537
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 5 cut(s) 114, 135, 1314, 1379, 1579
BssMI GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
BssT1I CCWWGG 3 cut(s) 135, 1314, 1379
Bst2UI CCWGG 3 cut(s) 1353, 1581, 1620
Bst4CI ACNGT 1 cut(s) 1396
Bst6I CTCTTC 2 cut(s) 100, 1234
BstC8I GCNNGC 2 cut(s) 19, 1515
BstDEI CTNAG 7 cut(s) 50, 345, 795, 951, 1125, 1175, 1563
BstEII GGTNACC 2 cut(s) 84, 1601
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 3 cut(s) 391, 904, 1525
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 8 cut(s) 242, 781, 826, 986, 1021, 1102, 1552, 1592
BstMAI GTCTC 4 cut(s) 152, 341, 851, 1178
BstMBI GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
BstMWI GCNNNNNNNGC 2 cut(s) 200, 367
BstNI CCWGG 3 cut(s) 1353, 1581, 1620
BstNSI RCATGY 2 cut(s) 21, 657
BstPI GGTNACC 2 cut(s) 84, 1601
BstSCI CCNGG 3 cut(s) 1351, 1579, 1618
BstSFI CTRYAG 3 cut(s) 195, 714, 1040
BstV1I GCAGC 2 cut(s) 343, 728
BstV2I GAAGAC 2 cut(s) 158, 873
BstX2I RGATCY 2 cut(s) 983, 1589
BstXI CCANNNNNNTGG 2 cut(s) 268, 1532
BstYI RGATCY 2 cut(s) 983, 1589
Bsu36I CCTNAGG 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 1493
BsuRI GGCC 6 cut(s) 305, 701, 928, 1123, 1524, 1555
BtsCI GGATG 3 cut(s) 391, 904, 1525
BtsI GCAGTG 1 cut(s) 1300
BtsIMutI CAGTG 2 cut(s) 1300, 1530
BveI ACCTGC 1 cut(s) 1518
Cac8I GCNNGC 2 cut(s) 19, 1515
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 5 cut(s) 258, 1554, 1567, 1616, 1622
CseI GACGC 1 cut(s) 776
Csp6I GTAC 3 cut(s) 123, 651, 741
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 6 cut(s) 18, 416, 638, 654, 1022, 1103
CviQI GTAC 3 cut(s) 123, 651, 741
DdeI CTNAG 7 cut(s) 50, 345, 795, 951, 1125, 1175, 1563
DpnI GATC 8 cut(s) 241, 780, 825, 985, 1020, 1101, 1551, 1591
DpnII GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
EaeI YGGCCR 1 cut(s) 1522
Eam1104I CTCTTC 2 cut(s) 100, 1234
EarI CTCTTC 2 cut(s) 100, 1234
Eco130I CCWWGG 3 cut(s) 135, 1314, 1379
Eco147I AGGCCT 2 cut(s) 305, 1123
Eco31I GGTCTC 2 cut(s) 152, 851
Eco32I GATATC 2 cut(s) 1155, 1459
Eco47I GGWCC 4 cut(s) 258, 1567, 1616, 1622
Eco81I CCTNAGG 1 cut(s) 1125
Eco91I GGTNACC 2 cut(s) 84, 1601
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoO65I GGTNACC 2 cut(s) 84, 1601
EcoRII CCWGG 3 cut(s) 1351, 1579, 1618
EcoRV GATATC 2 cut(s) 1155, 1459
EcoT14I CCWWGG 3 cut(s) 135, 1314, 1379
ErhI CCWWGG 3 cut(s) 135, 1314, 1379
FaeI CATG 6 cut(s) 21, 419, 641, 657, 1025, 1106
FalI AAGNNNNNCTT 2 cut(s) 689, 721
FatI CATG 6 cut(s) 17, 415, 637, 653, 1021, 1102
FauI CCCGC 1 cut(s) 1074
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 1 cut(s) 1099
Fnu4HI GCNGC 2 cut(s) 332, 717
FokI GGATG 3 cut(s) 398, 911, 1532
Fsp4HI GCNGC 2 cut(s) 332, 717
FspBI CTAG 8 cut(s) 120, 126, 285, 327, 479, 605, 729, 1055
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 2 cut(s) 332, 717
GsaI CCCAGC 1 cut(s) 1162
GsuI CTGGAG 1 cut(s) 304
HaeIII GGCC 6 cut(s) 305, 701, 928, 1123, 1524, 1555
HapII CCGG 3 cut(s) 256, 278, 525
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 34
Hin1II CATG 6 cut(s) 21, 419, 641, 657, 1025, 1106
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 3 cut(s) 376, 511, 726
HindII GTYRAC 3 cut(s) 376, 511, 726
HinfI GANTC 9 cut(s) 220, 298, 349, 528, 601, 1058, 1287, 1448, 1471
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 3 cut(s) 256, 278, 525
HphI GGTGA 4 cut(s) 78, 90, 215, 1453
Hpy166II GTNNAC 9 cut(s) 261, 376, 430, 511, 726, 734, 1403, 1570, 1616
Hpy188I TCNGA 5 cut(s) 346, 798, 970, 1099, 1308
Hpy188III TCNNGA 8 cut(s) 217, 310, 365, 404, 479, 525, 605, 1013
Hpy8I GTNNAC 9 cut(s) 261, 376, 430, 511, 726, 734, 1403, 1570, 1616
HpyAV CCTTC 4 cut(s) 259, 316, 814, 1233
HpyCH4III ACNGT 1 cut(s) 1396
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 367
HpyF3I CTNAG 7 cut(s) 50, 345, 795, 951, 1125, 1175, 1563
Hsp92II CATG 6 cut(s) 21, 419, 641, 657, 1025, 1106
HspAI GCGC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 1099
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
LmnI GCTCC 6 cut(s) 116, 286, 323, 1218, 1348, 1378
Lsp1109I GCAGC 2 cut(s) 343, 728
LweI GCATC 3 cut(s) 168, 493, 920
MaeI CTAG 8 cut(s) 120, 126, 285, 327, 479, 605, 729, 1055
MaeIII GTNAC 4 cut(s) 84, 946, 1006, 1601
MalI GATC 8 cut(s) 241, 780, 825, 985, 1020, 1101, 1551, 1591
MboI GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
MflI RGATCY 2 cut(s) 983, 1589
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 1 cut(s) 1524
MluCI AATT 4 cut(s) 690, 857, 976, 1076
MluNI TGGCCA 1 cut(s) 1524
MlyI GAGTC 2 cut(s) 343, 1296
MmeI TCCRAC 1 cut(s) 613
Mox20I TGGCCA 1 cut(s) 1524
MroI TCCGGA 1 cut(s) 524
MscI TGGCCA 1 cut(s) 1524
MseI TTAA 3 cut(s) 515, 725, 747
MslI CAYNNNNRTG 2 cut(s) 266, 435
Msp20I TGGCCA 1 cut(s) 1524
MspA1I CMGCKG 2 cut(s) 1478, 1628
MspI CCGG 3 cut(s) 256, 278, 525
MspR9I CCNGG 3 cut(s) 1353, 1581, 1620
MvaI CCWGG 3 cut(s) 1353, 1581, 1620
MwoI GCNNNNNNNGC 2 cut(s) 200, 367
NdeI CATATG 1 cut(s) 664
NdeII GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
NlaIII CATG 6 cut(s) 21, 419, 641, 657, 1025, 1106
NlaIV GGNNCC 4 cut(s) 112, 282, 523, 1344
NmeAIII GCCGAG 2 cut(s) 95, 828
NmuCI GTSAC 2 cut(s) 84, 1006
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 2 cut(s) 21, 657
OliI CACNNNNGTG 2 cut(s) 266, 435
PaeI GCATGC 1 cut(s) 21
PceI AGGCCT 2 cut(s) 305, 1123
PciI ACATGT 1 cut(s) 653
PfeI GAWTC 7 cut(s) 220, 298, 528, 601, 1058, 1448, 1471
PflMI CCANNNNNTGG 1 cut(s) 1586
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 2 cut(s) 333, 718
PleI GAGTC 2 cut(s) 343, 1295
PpsI GAGTC 2 cut(s) 343, 1295
PscI ACATGT 1 cut(s) 653
Psp6I CCWGG 3 cut(s) 1351, 1579, 1618
PspEI GGTNACC 2 cut(s) 84, 1601
PspFI CCCAGC 1 cut(s) 1158
PspGI CCWGG 3 cut(s) 1351, 1579, 1618
PspN4I GGNNCC 4 cut(s) 112, 282, 523, 1344
PspPI GGNCC 5 cut(s) 258, 1554, 1567, 1616, 1622
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 2 cut(s) 983, 1589
PvuII CAGCTG 2 cut(s) 1478, 1628
RsaI GTAC 3 cut(s) 124, 652, 742
RsaNI GTAC 3 cut(s) 123, 651, 741
RseI CAYNNNNRTG 2 cut(s) 266, 435
SaqAI TTAA 3 cut(s) 515, 725, 747
SatI GCNGC 2 cut(s) 332, 717
Sau3AI GATC 8 cut(s) 239, 778, 823, 983, 1018, 1099, 1549, 1589
Sau96I GGNCC 5 cut(s) 258, 1554, 1567, 1616, 1622
ScaI AGTACT 2 cut(s) 124, 742
SchI GAGTC 2 cut(s) 343, 1296
ScrFI CCNGG 3 cut(s) 1353, 1581, 1620
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 3 cut(s) 168, 493, 920
SfcI CTRYAG 3 cut(s) 195, 714, 1040
SinI GGWCC 4 cut(s) 258, 1567, 1616, 1622
SmiMI CAYNNNNRTG 2 cut(s) 266, 435
SmlI CTYRAG 4 cut(s) 365, 596, 1011, 1117
SmoI CTYRAG 4 cut(s) 365, 596, 1011, 1117
SpeI ACTAGT 1 cut(s) 728
SphI GCATGC 1 cut(s) 21
Sse9I AATT 4 cut(s) 690, 857, 976, 1076
SseBI AGGCCT 2 cut(s) 305, 1123
SsiI CCGC 5 cut(s) 203, 994, 1067, 1253, 1517
SspMI CTAG 8 cut(s) 120, 126, 285, 327, 479, 605, 729, 1055
StuI AGGCCT 2 cut(s) 305, 1123
StyD4I CCNGG 3 cut(s) 1351, 1579, 1618
StyI CCWWGG 3 cut(s) 135, 1314, 1379
TaaI ACNGT 1 cut(s) 1396
TaqI TCGA 2 cut(s) 773, 1084
TasI AATT 4 cut(s) 690, 857, 976, 1076
TatI WGTACW 2 cut(s) 122, 740
TfiI GAWTC 7 cut(s) 220, 298, 528, 601, 1058, 1448, 1471
Tru1I TTAA 3 cut(s) 515, 725, 747
Tru9I TTAA 3 cut(s) 515, 725, 747
TscAI CASTG 2 cut(s) 1300, 1537
TseFI GTSAC 2 cut(s) 84, 1006
TseI GCWGC 2 cut(s) 331, 716
Tsp45I GTSAC 2 cut(s) 84, 1006
TspDTI ATGAA 3 cut(s) 697, 991, 1531
TspGWI ACGGA 3 cut(s) 447, 996, 1562
TspRI CASTG 2 cut(s) 1300, 1537
Van91I CCANNNNNTGG 1 cut(s) 1586
VpaK11BI GGWCC 4 cut(s) 258, 1567, 1616, 1622
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 1 cut(s) 857
XbaI TCTAGA 2 cut(s) 478, 604
XceI RCATGY 2 cut(s) 21, 657
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XspI CTAG 8 cut(s) 120, 126, 285, 327, 479, 605, 729, 1055
ZrmI AGTACT 2 cut(s) 124, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.