MD13G1061500.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012513
Physical Location & Seq
Reverse (-)
4315870 .. 4318630
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1061500.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGGGAACTCAATGGCATGCTTCTTGCCGGCGCACAAGCCCGGAAACTTGACCCGAAACCCCTCGAAGCGGCCAACGTACTCTTCGCCGTCCTTGGCTTCCGCTGGTAGTACTAGAAGATCGTCAAACGGTTCGACCAAGAAGAAAGAGCGGCTTTCTGATGCTGATGACTTGTTGCTTCAGCAACAAGCTATAGAGGCTGTTCTGCTGTTCCAGAACGTCCAGAAGAACGGCGGTTCTTCGCAACTGTTTAACCGGTCCACTTCCGTTGTGTATCCCTCTCCGGCTCCTAAGAATCAGGGGTTTGCGAAGAGCTCGAGCTCTAGGCAGCGTTTGCGGTCGGATACTCTCGGATTTCAGCCTCTCGAGCTTGTTGATAAGCAGGACGTGAAGACTGATGAACTGGAAACCAATCATTTTATCCTAGTTCATGGAGGTGGCTTTGGTGCTTGGTGCTGGTACAAAACCATGACACTTTTGGAAGAAAGTGGGTTCAAAGTTGATGCAGTTGACCTGACCGGTTCTGGAGTTCACTCATTTGATACAAACAACATCACAAGTCTTGCACAATATGTCAAGCCACTCACTGACATCCTTGAGAATCTTGGGGTTGGGAAGAAGGTGATTTTGGTTGGACATGATTTTGGTGGCACATGTATCTCATATGCAATGGAGTTGTTTCCATCTAAAATTGCAAAAGCCATATTCATTTGTGCAGCAATGTTAACCAGTGGGCAGACCACTCTTAATCTCTTTGCTCAACAGACCGGATCGAACGATCTCATGCGACAGGCTCAGAGATTCTTGTATGCCAATGGGAAGGATCAGCCTCCTAGTGCTATTACTCTTGACAAAAAACTGTCCGAAGACTTATTGTTCAATCAAAGTCCTGCAAAGGATGTTGCACTGGCATCGGTCTCAATGAGGCCAATCCCCTTTGCACCAGTAACAGAGAAGCTTTTTCTTTCCGACGAGAACTACGGCGCTGTCAGGCGGTTTTTCGTAGTGACTCAAGAAGATCATGCCATAACCGTTCCATTACAAGAAGCCATGATCGAATCCAACCTGCCGGAACAGGTTTTCCGGCTTAAGGGCTCCGATCATGCACCGTTTTTCTCAAGGCCACAGGCTTTGCATAGGATATTAGTAGAGATATCACAACTTCCTCCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

392

Amino Acids

42.89

Weight (kDa)

8.54

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 138 - 373 2.1e-17 alpha/beta hydrolase fold
Hydrolase_4 PF12146 140 - 250 2.8e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 141 - 378 1.8e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1074
AccBSI CCGCTC 1 cut(s) 151
AciI CCGC 6 cut(s) 70, 102, 151, 234, 337, 994
AclWI GGATC 2 cut(s) 778, 831
AcoI YGGCCR 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 3 cut(s) 80, 112, 461
AfiI CCNNNNNNNGG 2 cut(s) 69, 1090
AflII CTTAAG 1 cut(s) 1088
AflIII ACRYGT 1 cut(s) 653
AgeI ACCGGT 2 cut(s) 255, 518
AgsI TTSAA 2 cut(s) 496, 880
AjiI CACGTC 1 cut(s) 388
AjuI GAANNNNNNNTTGG 4 cut(s) 67, 99, 611, 643
AloI GAACNNNNNNTCC 2 cut(s) 1065, 1097
AluBI AGCT 5 cut(s) 191, 315, 321, 370, 958
AluI AGCT 5 cut(s) 191, 315, 321, 370, 958
Alw21I GWGCWC 2 cut(s) 317, 323
Alw26I GTCTC 1 cut(s) 922
AlwI GGATC 2 cut(s) 778, 831
Ama87I CYCGRG 2 cut(s) 316, 365
AoxI GGCC 3 cut(s) 71, 926, 1121
ApeKI GCWGC 2 cut(s) 328, 716
AsiGI ACCGGT 2 cut(s) 255, 518
AspLEI GCGC 2 cut(s) 34, 986
AspS9I GGNCC 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 634
AvaI CYCGRG 2 cut(s) 316, 365
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 3 cut(s) 317, 323, 1097
BbsI GAAGAC 2 cut(s) 398, 873
Bbv12I GWGCWC 2 cut(s) 317, 323
BbvI GCAGC 2 cut(s) 340, 728
BccI CCATC 1 cut(s) 691
BceAI ACGGC 3 cut(s) 73, 247, 997
BcgI CGANNNNNNTGC 2 cut(s) 894, 928
BciVI GTATCC 2 cut(s) 285, 337
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 922
BfaI CTAG 4 cut(s) 114, 324, 425, 834
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 987
BfrI CTTAAG 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 1074
BfuI GTATCC 2 cut(s) 285, 337
BisI GCNGC 4 cut(s) 71, 152, 329, 717
BlsI GCNGC 4 cut(s) 72, 153, 330, 718
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 258
BmeT110I CYCGRG 2 cut(s) 316, 365
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 1 cut(s) 258
BmiI GGNNCC 2 cut(s) 288, 1096
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 3 cut(s) 151, 493, 920
BpiI GAAGAC 2 cut(s) 398, 873
BpmI CTGGAG 2 cut(s) 546, 1152
BpuEI CTTGAG 3 cut(s) 617, 996, 1102
BpuMI CCSGG 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 3 cut(s) 255, 518, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 1090
Bse118I RCCGGY 3 cut(s) 28, 255, 518
Bse1I ACTGG 5 cut(s) 408, 729, 912, 944, 1169
Bse3DI GCAATG 2 cut(s) 675, 726
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 591, 904
BseLI CCNNNNNNNGG 2 cut(s) 69, 1090
BseMI GCAATG 2 cut(s) 675, 726
BseMII CTCAG 1 cut(s) 809
BseNI ACTGG 5 cut(s) 408, 729, 912, 944, 1169
BseXI GCAGC 2 cut(s) 340, 728
BsgI GTGCAG 1 cut(s) 735
Bsh1285I CGRYCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 73, 928, 1123
BshTI ACCGGT 2 cut(s) 255, 518
BsiEI CGRYCG 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 317, 323
BsiHKCI CYCGRG 2 cut(s) 316, 365
BsiSI CCGG 8 cut(s) 29, 42, 256, 284, 519, 768, 1070, 1084
BslI CCNNNNNNNGG 2 cut(s) 69, 1090
BsmAI GTCTC 1 cut(s) 922
BsnI GGCC 3 cut(s) 73, 928, 1123
Bso31I GGTCTC 1 cut(s) 922
BsoBI CYCGRG 2 cut(s) 316, 365
Bsp1286I GDGCHC 3 cut(s) 317, 323, 1097
Bsp143I GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
BspACI CCGC 6 cut(s) 70, 102, 151, 234, 337, 994
BspANI GGCC 3 cut(s) 73, 928, 1123
BspCNI CTCAG 1 cut(s) 808
BspLI GGNNCC 2 cut(s) 288, 1096
BspMI ACCTGC 1 cut(s) 1074
BspPI GGATC 2 cut(s) 778, 831
BspQI GCTCTTC 1 cut(s) 305
BspTI CTTAAG 1 cut(s) 1088
BspTNI GGTCTC 1 cut(s) 922
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 2 cut(s) 675, 726
BsrFI RCCGGY 3 cut(s) 28, 255, 518
BsrI ACTGG 5 cut(s) 408, 729, 912, 944, 1169
BssAI RCCGGY 3 cut(s) 28, 255, 518
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 5 cut(s) 131, 249, 861, 1033, 1110
Bst6I CTCTTC 2 cut(s) 88, 305
BstAFI CTTAAG 1 cut(s) 1088
BstAPI GCANNNNNTGC 1 cut(s) 334
BstC8I GCNNGC 2 cut(s) 19, 30
BstDEI CTNAG 2 cut(s) 291, 795
BstF5I GGATG 2 cut(s) 591, 904
BstH2I RGCGCY 1 cut(s) 987
BstHHI GCGC 2 cut(s) 34, 986
BstKTI GATC 7 cut(s) 122, 773, 781, 826, 1021, 1056, 1102
BstMAI GTCTC 1 cut(s) 922
BstMBI GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
BstMCI CGRYCG 1 cut(s) 341
BstMWI GCNNNNNNNGC 3 cut(s) 197, 334, 367
BstNSI RCATGY 2 cut(s) 21, 657
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 340, 728
BstV2I GAAGAC 2 cut(s) 398, 873
BsuI GTATCC 2 cut(s) 285, 337
BsuRI GGCC 3 cut(s) 73, 928, 1123
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 2 cut(s) 591, 904
BtsIMutI CAGTG 4 cut(s) 585, 736, 905, 1176
BveI ACCTGC 1 cut(s) 1074
Cac8I GCNNGC 2 cut(s) 19, 30
CfoI GCGC 2 cut(s) 34, 986
Cfr10I RCCGGY 3 cut(s) 28, 255, 518
Cfr13I GGNCC 1 cut(s) 258
Csp6I GTAC 3 cut(s) 79, 111, 460
CspAI ACCGGT 2 cut(s) 255, 518
CspCI CAANNNNNGTGG 4 cut(s) 250, 285, 1113, 1148
CviAII CATG 9 cut(s) 18, 431, 469, 638, 654, 784, 1022, 1051, 1103
CviQI GTAC 3 cut(s) 79, 111, 460
DdeI CTNAG 2 cut(s) 291, 795
DpnI GATC 7 cut(s) 121, 772, 780, 825, 1020, 1055, 1101
DpnII GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
EaeI YGGCCR 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 88, 305
EarI CTCTTC 2 cut(s) 88, 305
Ecl136II GAGCTC 2 cut(s) 315, 321
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 3 cut(s) 317, 323, 1097
Eco31I GGTCTC 1 cut(s) 922
Eco32I GATATC 1 cut(s) 1155
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 2 cut(s) 315, 321
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 2 cut(s) 316, 365
EcoICRI GAGCTC 2 cut(s) 315, 321
EcoRV GATATC 1 cut(s) 1155
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 3 cut(s) 317, 323, 1097
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 9 cut(s) 21, 434, 472, 641, 657, 787, 1025, 1054, 1106
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 9 cut(s) 17, 430, 468, 637, 653, 783, 1021, 1050, 1102
FauNDI CATATG 1 cut(s) 664
Fnu4HI GCNGC 4 cut(s) 71, 152, 329, 717
FokI GGATG 2 cut(s) 578, 911
FriOI GRGCYC 3 cut(s) 317, 323, 1097
Fsp4HI GCNGC 4 cut(s) 71, 152, 329, 717
FspBI CTAG 4 cut(s) 114, 324, 425, 834
GlaI GCGC 2 cut(s) 33, 985
GluI GCNGC 4 cut(s) 71, 152, 329, 717
GsuI CTGGAG 2 cut(s) 546, 1152
HaeII RGCGCY 1 cut(s) 987
HaeIII GGCC 3 cut(s) 73, 928, 1123
HapII CCGG 8 cut(s) 29, 42, 256, 284, 519, 768, 1070, 1084
HhaI GCGC 2 cut(s) 34, 986
Hin1II CATG 9 cut(s) 21, 434, 472, 641, 657, 787, 1025, 1054, 1106
Hin6I GCGC 2 cut(s) 32, 984
HinP1I GCGC 2 cut(s) 32, 984
HincII GTYRAC 2 cut(s) 511, 726
HindII GTYRAC 2 cut(s) 511, 726
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 5 cut(s) 295, 601, 801, 1009, 1058
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 8 cut(s) 29, 42, 256, 284, 519, 768, 1070, 1084
HphI GGTGA 1 cut(s) 634
Hpy166II GTNNAC 4 cut(s) 261, 511, 532, 726
Hpy188I TCNGA 7 cut(s) 160, 343, 353, 798, 865, 970, 1099
Hpy188III TCNNGA 6 cut(s) 214, 223, 365, 525, 848, 1013
Hpy8I GTNNAC 4 cut(s) 261, 511, 532, 726
Hpy99I CGWCG 1 cut(s) 974
HpyAV CCTTC 2 cut(s) 613, 814
HpyCH4III ACNGT 5 cut(s) 131, 249, 861, 1033, 1110
HpyCH4IV ACGT 3 cut(s) 77, 219, 387
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 334, 367
HpyF3I CTNAG 2 cut(s) 291, 795
HpySE526I ACGT 3 cut(s) 77, 219, 387
Hsp92II CATG 9 cut(s) 21, 434, 472, 641, 657, 787, 1025, 1054, 1106
HspAI GCGC 2 cut(s) 32, 984
KroI GCCGGC 1 cut(s) 28
KroNI GCCGGC 1 cut(s) 30
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 2 cut(s) 292, 1100
Lsp1109I GCAGC 2 cut(s) 340, 728
LweI GCATC 3 cut(s) 151, 493, 920
MaeI CTAG 4 cut(s) 114, 324, 425, 834
MaeII ACGT 3 cut(s) 77, 219, 387
MaeIII GTNAC 2 cut(s) 946, 1006
MalI GATC 7 cut(s) 121, 772, 780, 825, 1020, 1055, 1101
MbiI CCGCTC 1 cut(s) 151
MboI GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
MhlI GDGCHC 3 cut(s) 317, 323, 1097
MluCI AATT 1 cut(s) 690
MlyI GAGTC 1 cut(s) 1003
MmeI TCCRAC 4 cut(s) 321, 613, 993, 1086
MnlI CCTC 8 cut(s) 73, 190, 289, 372, 428, 840, 918, 1176
MroNI GCCGGC 1 cut(s) 28
MseI TTAA 4 cut(s) 252, 725, 747, 1089
MslI CAYNNNNRTG 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 1088
MspI CCGG 8 cut(s) 29, 42, 256, 284, 519, 768, 1070, 1084
MspR9I CCNGG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 197, 334, 367
NaeI GCCGGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 664
NdeII GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
NgoMIV GCCGGC 1 cut(s) 28
NlaIII CATG 9 cut(s) 21, 434, 472, 641, 657, 787, 1025, 1054, 1106
NlaIV GGNNCC 2 cut(s) 288, 1096
NmuCI GTSAC 1 cut(s) 1006
NspI RCATGY 2 cut(s) 21, 657
PaeI GCATGC 1 cut(s) 21
PaeR7I CTCGAG 2 cut(s) 316, 365
PciI ACATGT 1 cut(s) 653
PciSI GCTCTTC 1 cut(s) 305
PdiI GCCGGC 1 cut(s) 30
PfeI GAWTC 4 cut(s) 295, 601, 801, 1058
PinAI ACCGGT 2 cut(s) 255, 518
PkrI GCNGC 4 cut(s) 72, 153, 330, 718
PleI GAGTC 1 cut(s) 1003
PpsI GAGTC 1 cut(s) 1003
PscI ACATGT 1 cut(s) 653
Psp124BI GAGCTC 2 cut(s) 317, 323
PspN4I GGNNCC 2 cut(s) 288, 1096
PspPI GGNCC 1 cut(s) 258
PspXI VCTCGAGB 1 cut(s) 316
RsaI GTAC 3 cut(s) 80, 112, 461
RsaNI GTAC 3 cut(s) 79, 111, 460
RseI CAYNNNNRTG 1 cut(s) 435
SacI GAGCTC 2 cut(s) 317, 323
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 4 cut(s) 252, 725, 747, 1089
SatI GCNGC 4 cut(s) 71, 152, 329, 717
Sau3AI GATC 7 cut(s) 119, 770, 778, 823, 1018, 1053, 1099
Sau96I GGNCC 1 cut(s) 258
ScaI AGTACT 1 cut(s) 112
SchI GAGTC 1 cut(s) 1003
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 3 cut(s) 317, 323, 1097
SfaNI GCATC 3 cut(s) 151, 493, 920
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 2 cut(s) 316, 365
SinI GGWCC 1 cut(s) 258
SlaI CTCGAG 2 cut(s) 316, 365
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 6 cut(s) 316, 365, 596, 1011, 1088, 1117
SmoI CTYRAG 6 cut(s) 316, 365, 596, 1011, 1088, 1117
SphI GCATGC 1 cut(s) 21
Sse9I AATT 1 cut(s) 690
SsiI CCGC 6 cut(s) 70, 102, 151, 234, 337, 994
SspMI CTAG 4 cut(s) 114, 324, 425, 834
SstI GAGCTC 2 cut(s) 317, 323
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 5 cut(s) 131, 249, 861, 1033, 1110
TaiI ACGT 3 cut(s) 80, 222, 390
TaqI TCGA 6 cut(s) 65, 134, 317, 366, 773, 1056
TaqII GACCGA 1 cut(s) 904
TasI AATT 1 cut(s) 690
TatI WGTACW 1 cut(s) 110
TauI GCSGC 2 cut(s) 73, 154
TfiI GAWTC 4 cut(s) 295, 601, 801, 1058
Tru1I TTAA 4 cut(s) 252, 725, 747, 1089
Tru9I TTAA 4 cut(s) 252, 725, 747, 1089
TscAI CASTG 4 cut(s) 592, 736, 912, 1176
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 2 cut(s) 328, 716
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 3 cut(s) 414, 419, 697
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 4 cut(s) 592, 736, 912, 1176
Vha464I CTTAAG 1 cut(s) 1088
VpaK11BI GGWCC 1 cut(s) 258
XceI RCATGY 2 cut(s) 21, 657
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XhoI CTCGAG 2 cut(s) 316, 365
XspI CTAG 4 cut(s) 114, 324, 425, 834
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.