Rh4DG364300

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012513
Physical Location & Seq
Reverse (-)
58534117 .. 58536509
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG364300.1

Sequence Viewer

Length: 1185 bp
ATGGGAAACTCAATGGCATGCTTCTCCCCTGCGCACATACCAAAGAAGCTGAGCACCAACTTGTCAAGGAAGCCCTCCAAGAGGTCACCCAACTCTTCACCTTTCTTTGGCTCCTCGGCTAGTACTAGAAGAACCAAGAGTTCTCCCAAGAAGACGTCACAGACCCTTGCTGATGATTTGATGCTTCAACAACAAGCTATAGAGGCGGTTCTGTTGTTCCAGAACCACCAGAAGAACAATGGATCGCAACCCTTCAACCGGTCCACCTCTGTGGTGTTTCCGGCTCCTGGACCAAAGAAGAATCAGGCCTTCACGAAAAGCTCCAGCTCTAGGCAGCCTTTGAGTTCTGAGACTCTCTTTCAGCCTCTTGAGCTTGTTGACAAGCAGGATGTAAAGATTGATGGTCTGGAAACAAATCATTTTGTACTTGTTCACGGAGGTGGCTTTGGTGCTTGGTGCTGGTATAAAACAATGACACTTCTAGAAGAAAGTGGGTTCAAGGTTGATGCAGTTGACTTAACTGGTTCCGGAATCCACTCATTTGATACAAACAGCATTACAAGTCTTGCACAATATGTGAAGCCACTTACTGAAATCCTTGAGAATCTAGAGGAGGGGAAAAAGGTCATTTTGGTTGGACATGATTTTGGTGGTACGTGTATCTCATATGCAATGGAGTTGTTTCCATCTAAAATTGCAAAGGCCATCTTCATATCTGCAGCAATGTTAACTAGTGGGCAGAGTACTCTTAATCTGTTTGCTCAACAGACAGGCTCGAACGATCTGATGCGTCAGGCTCAGATATTCTTGTATGCAAATGGGAAGGATCAGCCTCCTACTGCTATTGGTCTCGGCAAAAAATTTACCGAAGACTTATTGTTCAATCAAAGTCCAGCAAAGGATGTTGCATTGGCATCAGTATCAATGAGGCCAATCCCTTTTGCACCAGTAACTGAGAAGCTCTCTCTTTCTGACAAGAATTACGGATCTGTCAGGCGGTTTTTCATAGTCACTCAAGAAGATCATGCCATCAGCGTTCCACTACAGGAGGCAATGCTAGAATCAAACCCGCCAGAACAGTTTTATCGACTAAAAGGCTCTGATCATGCACCTTTTTTCTCGAGGCCTCAGGCTTTGCATAGGATATTAGTAGAGATATCCCAGGTTCCACCTAATCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

394

Amino Acids

43.25

Weight (kDa)

9.06

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 139 - 306 4.1e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 379 9.8e-20 Alpha/beta hydrolase family
Hydrolase_4 PF12146 141 - 252 1.2e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 158
Acc16I TGCGCA 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 527
AciI CCGC 3 cut(s) 206, 997, 1070
AclWI GGATC 3 cut(s) 250, 834, 994
AcsI RAATTY 1 cut(s) 860
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 4 cut(s) 124, 426, 655, 745
AfiI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
AflIII ACRYGT 1 cut(s) 656
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 4 cut(s) 188, 256, 499, 883
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 2 cut(s) 286, 1161
AleI CACNNNNGTG 2 cut(s) 269, 438
AluBI AGCT 6 cut(s) 49, 197, 321, 327, 373, 961
AluI AGCT 6 cut(s) 49, 197, 321, 327, 373, 961
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 344, 854
AlwI GGATC 3 cut(s) 250, 834, 994
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 1 cut(s) 1120
Aor13HI TCCGGA 1 cut(s) 527
AoxI GGCC 4 cut(s) 306, 702, 929, 1124
ApeKI GCWGC 2 cut(s) 334, 719
ApoI RAATTY 1 cut(s) 860
AsiGI ACCGGT 1 cut(s) 258
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 261, 290
AsuHPI GGTGA 2 cut(s) 78, 90
AvaI CYCGRG 1 cut(s) 1120
AvaII GGWCC 2 cut(s) 261, 290
AxyI CCTNAGG 1 cut(s) 1128
BbsI GAAGAC 2 cut(s) 158, 876
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 346, 731
BccI CCATC 4 cut(s) 395, 694, 713, 1037
BciT130I CCWGG 2 cut(s) 288, 1163
BclI TGATCA 1 cut(s) 1102
BcoDI GTCTC 2 cut(s) 344, 854
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 7 cut(s) 120, 126, 330, 482, 608, 732, 1058
BfmI CTRYAG 3 cut(s) 198, 717, 1043
BisI GCNGC 2 cut(s) 335, 720
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 2 cut(s) 336, 721
BmcAI AGTACT 2 cut(s) 124, 745
Bme1390I CCNGG 2 cut(s) 288, 1163
Bme18I GGWCC 2 cut(s) 261, 290
BmeT110I CYCGRG 1 cut(s) 1120
BmgT120I GGNCC 2 cut(s) 261, 290
BmiI GGNNCC 4 cut(s) 112, 285, 526, 1167
BmrFI CCNGG 2 cut(s) 288, 1163
BmsI GCATC 4 cut(s) 171, 496, 777, 923
BpiI GAAGAC 2 cut(s) 158, 876
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 3 cut(s) 389, 620, 999
BsaAI YACGTR 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 114, 1161
BsaWI WCCGGW 2 cut(s) 258, 527
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 2 cut(s) 526, 947
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 3 cut(s) 678, 729, 1059
BseAI TCCGGA 1 cut(s) 527
BseBI CCWGG 2 cut(s) 288, 1163
BseDI CCNNGG 2 cut(s) 114, 1161
BseGI GGATG 2 cut(s) 394, 907
BseLI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
BseMI GCAATG 3 cut(s) 678, 729, 1059
BseMII CTCAG 6 cut(s) 41, 339, 812, 945, 1142, 1169
BseNI ACTGG 2 cut(s) 526, 947
BseRI GAGGAG 2 cut(s) 103, 626
BseXI GCAGC 2 cut(s) 346, 731
BshFI GGCC 4 cut(s) 308, 704, 931, 1126
BshTI ACCGGT 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 1120
BsiSI CCGG 3 cut(s) 259, 281, 528
BslI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
BsmAI GTCTC 2 cut(s) 344, 854
BsnI GGCC 4 cut(s) 308, 704, 931, 1126
Bso31I GGTCTC 1 cut(s) 854
BsoBI CYCGRG 1 cut(s) 1120
Bsp1286I GDGCHC 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 527
Bsp143I GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 3 cut(s) 206, 997, 1070
BspANI GGCC 4 cut(s) 308, 704, 931, 1126
BspCNI CTCAG 6 cut(s) 42, 340, 811, 946, 1141, 1170
BspEI TCCGGA 1 cut(s) 527
BspLI GGNNCC 4 cut(s) 112, 285, 526, 1167
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 3 cut(s) 250, 834, 994
BspTNI GGTCTC 1 cut(s) 854
BsrDI GCAATG 3 cut(s) 678, 729, 1059
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 2 cut(s) 526, 947
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 2 cut(s) 114, 1161
BssMI GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
BssNI GRCGYC 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 288, 1163
Bst4CI ACNGT 1 cut(s) 1080
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 657
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 6 cut(s) 50, 348, 798, 954, 1128, 1178
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 394, 907
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 6 cut(s) 245, 784, 829, 989, 1024, 1105
BstMAI GTCTC 2 cut(s) 344, 854
BstMBI GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 203, 370
BstNI CCWGG 2 cut(s) 288, 1163
BstNSI RCATGY 1 cut(s) 21
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 2 cut(s) 286, 1161
BstSFI CTRYAG 3 cut(s) 198, 717, 1043
BstV1I GCAGC 2 cut(s) 346, 731
BstV2I GAAGAC 2 cut(s) 158, 876
BstX2I RGATCY 1 cut(s) 986
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 1 cut(s) 986
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 4 cut(s) 308, 704, 931, 1126
BtsCI GGATG 2 cut(s) 394, 907
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 953
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 261, 290
CseI GACGC 1 cut(s) 779
Csp6I GTAC 4 cut(s) 123, 425, 654, 744
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 4 cut(s) 18, 641, 1025, 1106
CviQI GTAC 4 cut(s) 123, 425, 654, 744
DdeI CTNAG 6 cut(s) 50, 348, 798, 954, 1128, 1178
DpnI GATC 6 cut(s) 244, 783, 828, 988, 1023, 1104
DpnII GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco147I AGGCCT 2 cut(s) 308, 1126
Eco31I GGTCTC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 1158
Eco47I GGWCC 2 cut(s) 261, 290
Eco81I CCTNAGG 1 cut(s) 1128
Eco88I CYCGRG 1 cut(s) 1120
Eco91I GGTNACC 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 286, 1161
EcoRV GATATC 1 cut(s) 1158
FaeI CATG 4 cut(s) 21, 644, 1028, 1109
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FatI CATG 4 cut(s) 17, 640, 1024, 1105
FauI CCCGC 1 cut(s) 1077
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 1 cut(s) 1102
Fnu4HI GCNGC 2 cut(s) 335, 720
FokI GGATG 2 cut(s) 401, 914
Fsp4HI GCNGC 2 cut(s) 335, 720
FspBI CTAG 7 cut(s) 120, 126, 330, 482, 608, 732, 1058
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 2 cut(s) 335, 720
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 4 cut(s) 308, 704, 931, 1126
HapII CCGG 3 cut(s) 259, 281, 528
HgaI GACGC 1 cut(s) 779
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 4 cut(s) 21, 644, 1028, 1109
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 3 cut(s) 379, 514, 729
HindII GTYRAC 3 cut(s) 379, 514, 729
HinfI GANTC 5 cut(s) 301, 352, 531, 604, 1061
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 3 cut(s) 259, 281, 528
HphI GGTGA 2 cut(s) 78, 90
Hpy166II GTNNAC 5 cut(s) 264, 379, 433, 514, 729
Hpy188I TCNGA 6 cut(s) 349, 786, 801, 973, 1102, 1179
Hpy188III TCNNGA 9 cut(s) 220, 313, 368, 407, 482, 528, 608, 1016, 1120
Hpy8I GTNNAC 5 cut(s) 264, 379, 433, 514, 729
HpyAV CCTTC 3 cut(s) 262, 319, 817
HpyCH4III ACNGT 1 cut(s) 1080
HpyCH4IV ACGT 2 cut(s) 155, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 370
HpyF3I CTNAG 6 cut(s) 50, 348, 798, 954, 1128, 1178
HpySE526I ACGT 2 cut(s) 155, 656
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 4 cut(s) 21, 644, 1028, 1109
HspAI GCGC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 527
Ksp22I TGATCA 1 cut(s) 1102
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
LmnI GCTCC 3 cut(s) 116, 289, 326
Lsp1109I GCAGC 2 cut(s) 346, 731
LweI GCATC 4 cut(s) 171, 496, 777, 923
MaeI CTAG 7 cut(s) 120, 126, 330, 482, 608, 732, 1058
MaeII ACGT 2 cut(s) 155, 656
MaeIII GTNAC 4 cut(s) 84, 156, 949, 1009
MalI GATC 6 cut(s) 244, 783, 828, 988, 1023, 1104
MboI GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
MboII GAAGA 9 cut(s) 87, 141, 163, 244, 310, 497, 700, 881, 1031
MflI RGATCY 1 cut(s) 986
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 693, 860, 979
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 616
MroI TCCGGA 1 cut(s) 527
MseI TTAA 3 cut(s) 518, 728, 750
MslI CAYNNNNRTG 2 cut(s) 269, 438
MspI CCGG 3 cut(s) 259, 281, 528
MspR9I CCNGG 2 cut(s) 288, 1163
MvaI CCWGG 2 cut(s) 288, 1163
MwoI GCNNNNNNNGC 2 cut(s) 203, 370
NdeI CATATG 1 cut(s) 667
NdeII GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
NlaIII CATG 4 cut(s) 21, 644, 1028, 1109
NlaIV GGNNCC 4 cut(s) 112, 285, 526, 1167
NmeAIII GCCGAG 2 cut(s) 95, 831
NmuCI GTSAC 3 cut(s) 84, 156, 1009
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 1 cut(s) 21
OliI CACNNNNGTG 2 cut(s) 269, 438
PaeI GCATGC 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 1120
PceI AGGCCT 2 cut(s) 308, 1126
PfeI GAWTC 4 cut(s) 301, 531, 604, 1061
PfoI TCCNGGA 1 cut(s) 286
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 2 cut(s) 336, 721
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 286, 1161
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 286, 1161
PspN4I GGNNCC 4 cut(s) 112, 285, 526, 1167
PspPI GGNCC 2 cut(s) 261, 290
PstI CTGCAG 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 1 cut(s) 986
RsaI GTAC 4 cut(s) 124, 426, 655, 745
RsaNI GTAC 4 cut(s) 123, 425, 654, 744
RseI CAYNNNNRTG 2 cut(s) 269, 438
SaqAI TTAA 3 cut(s) 518, 728, 750
SatI GCNGC 2 cut(s) 335, 720
Sau3AI GATC 6 cut(s) 242, 781, 826, 986, 1021, 1102
Sau96I GGNCC 2 cut(s) 261, 290
ScaI AGTACT 2 cut(s) 124, 745
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 288, 1163
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 4 cut(s) 171, 496, 777, 923
SfcI CTRYAG 3 cut(s) 198, 717, 1043
Sfr274I CTCGAG 1 cut(s) 1120
SinI GGWCC 2 cut(s) 261, 290
SlaI CTCGAG 1 cut(s) 1120
SmiMI CAYNNNNRTG 2 cut(s) 269, 438
SmlI CTYRAG 4 cut(s) 368, 599, 1014, 1120
SmoI CTYRAG 4 cut(s) 368, 599, 1014, 1120
SpeI ACTAGT 1 cut(s) 731
SphI GCATGC 1 cut(s) 21
Sse9I AATT 3 cut(s) 693, 860, 979
SseBI AGGCCT 2 cut(s) 308, 1126
SsiI CCGC 3 cut(s) 206, 997, 1070
SspMI CTAG 7 cut(s) 120, 126, 330, 482, 608, 732, 1058
StuI AGGCCT 2 cut(s) 308, 1126
StyD4I CCNGG 2 cut(s) 286, 1161
TaaI ACNGT 1 cut(s) 1080
TaiI ACGT 2 cut(s) 158, 659
TaqI TCGA 3 cut(s) 776, 1087, 1121
TasI AATT 3 cut(s) 693, 860, 979
TatI WGTACW 3 cut(s) 122, 424, 743
TfiI GAWTC 4 cut(s) 301, 531, 604, 1061
Tru1I TTAA 3 cut(s) 518, 728, 750
Tru9I TTAA 3 cut(s) 518, 728, 750
TseFI GTSAC 3 cut(s) 84, 156, 1009
TseI GCWGC 2 cut(s) 334, 719
Tsp45I GTSAC 3 cut(s) 84, 156, 1009
TspDTI ATGAA 2 cut(s) 700, 994
TspGWI ACGGA 2 cut(s) 450, 999
VpaK11BI GGWCC 2 cut(s) 261, 290
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 1 cut(s) 860
XbaI TCTAGA 2 cut(s) 481, 607
XceI RCATGY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 1120
XspI CTAG 7 cut(s) 120, 126, 330, 482, 608, 732, 1058
ZraI GACGTC 1 cut(s) 156
ZrmI AGTACT 2 cut(s) 124, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.