FvH4_4g29850

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012513
Physical Location & Seq
Reverse (-)
29789897 .. 29792333
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29850.t1

Sequence Viewer

Length: 1179 bp
ATGGGGAACTCAATGACATGCTTCTCCCCAGCACCAACGAAGCTGAGCACCAACTTGTCAAGAAGACCCTCCAAGAGGTCACCCTACTCTTCACCTTTCTTTGGCTCCTCAGCCAGTACTAGAAGAACAAAGGGCTCCCCCAAAAAGGTACAGACCCTTGAAGATGATCTGATCCTTCAGCAACAAGCTATCGAGGCAGTTCTGCTGTTCCAGAACCACCAGAAGAACAATGGTTCATTACCGCAGACCTTCAACCGCTCCACCTCCGTGGTGTTTCCGGCTCCTTCTGGACCAAAGAAGAATCAGGGCTTTACTAAAAGCTCCAGCTCCAGGCAGCCTTTGGGTTCTGATACTCTCTTTCAGCCTCTTGAGCTTGTTGACAAGCAGGATGTGAAGATTGAAGGTCTGGAAACGAATCATTTCGTACTTGTTCATGGAGGTGGGTTTGGTGCTTGGTGCTGGTATAAAACAATGACACTTCTGGAAGAAAGTGGGTTCAAAGTTGATGCAGTCGACTTAACTGGTTCTGGAATCCACTCATTCGATACAAACAGCATTACTAGTCTTGCACAATACGTGAAGCCACTTACTGAAATCCTTGAAAATCTAGACGAGGGCAAAAAGGTCATTTTGGCTGGACATGATTTTGGTGGTACGTGTATCTCATATGCAATGGAGTTGTTTCCGTCTAAAATTGCAAAGGCCATCTTCATATCTGCAGCAATGTTAACCAGTGGGCAGAGTACTCTTAATCTGTTTGCTCAACAGACAAGCTCGAATGATCTGATGCGACAGGCTCAGGTATTCTTGTATGCAAATGGGAAGGATCAGCCTCCTACAGCTATTGGTCTCGGCAAAAAATTTACTGAAGACTTGTTGTTCAATCAAAGTCCAGCAAAGGATGTTGCATTAGCATCAGTATCAATGAGGCCAATCCCTTTTGCACCAGTCACTGAGAAGCTCTCTCTTTCTGACAAGAATTACGGATCTGTTAGGCGGTTTTTCATAGTCACACAAGAAGATCATGCCATAAGTGTTGCACTACAGGAGGCAATGTTAGAATCAAATCCGCCGGAACAGTTTTATCGACTAAAAGGCTCTGATCATGCACCTTTTTTCTCAAGGCCTCAGGCTTTGCATAGGATATTAGTAGAGATATCCCAGGTTCCACCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

393

Amino Acids

43.0

Weight (kDa)

8.79

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 139 - 375 7.5e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 379 8.2e-19 Alpha/beta hydrolase family
Hydrolase_4 PF12146 141 - 255 1.8e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 513
AciI CCGC 4 cut(s) 242, 256, 997, 1070
AclWI GGATC 3 cut(s) 166, 834, 994
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 161, 888
AfaI GTAC 5 cut(s) 118, 150, 426, 655, 745
AfiI CCNNNNNNNGG 4 cut(s) 75, 101, 145, 330
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 6 cut(s) 161, 253, 401, 499, 602, 883
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 2 cut(s) 329, 1161
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 8 cut(s) 43, 188, 321, 327, 373, 774, 842, 961
AluI AGCT 8 cut(s) 43, 188, 321, 327, 373, 774, 842, 961
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 854
AlwI GGATC 3 cut(s) 166, 834, 994
AlwNI CAGNNNCTG 1 cut(s) 953
AoxI GGCC 3 cut(s) 702, 929, 1124
ApeKI GCWGC 2 cut(s) 334, 719
ApoI RAATTY 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 72, 84
AvaII GGWCC 1 cut(s) 290
AxyI CCTNAGG 1 cut(s) 1128
BanII GRGCYC 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 70, 876
Bbv12I GWGCWC 1 cut(s) 50
BbvCI CCTCAGC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 346, 731
BccI CCATC 1 cut(s) 713
BciT130I CCWGG 2 cut(s) 331, 1163
BclI TGATCA 1 cut(s) 1102
BcoDI GTCTC 1 cut(s) 854
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 3 cut(s) 120, 561, 608
BfmI CTRYAG 3 cut(s) 717, 837, 1043
BisI GCNGC 2 cut(s) 335, 720
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 2 cut(s) 336, 721
BmcAI AGTACT 2 cut(s) 118, 745
Bme1390I CCNGG 2 cut(s) 331, 1163
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 4 cut(s) 106, 136, 282, 1167
BmrFI CCNGG 2 cut(s) 331, 1163
BmsI GCATC 3 cut(s) 496, 777, 923
BpiI GAAGAC 2 cut(s) 70, 876
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 2 cut(s) 307, 313
Bpu10I CCTNAGC 2 cut(s) 109, 798
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 389, 1105
BsaAI YACGTR 2 cut(s) 577, 657
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 267, 1161
BsaXI ACNNNNNCTCC 2 cut(s) 1040, 1070
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 101, 145, 330
Bse1I ACTGG 4 cut(s) 114, 526, 732, 947
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 3 cut(s) 678, 729, 1059
BseBI CCWGG 2 cut(s) 331, 1163
BseDI CCNNGG 2 cut(s) 267, 1161
BseGI GGATG 2 cut(s) 394, 907
BseLI CCNNNNNNNGG 4 cut(s) 75, 101, 145, 330
BseMI GCAATG 3 cut(s) 678, 729, 1059
BseMII CTCAG 5 cut(s) 35, 123, 812, 945, 1142
BseNI ACTGG 4 cut(s) 114, 526, 732, 947
BseRI GAGGAG 1 cut(s) 97
BseXI GCAGC 2 cut(s) 346, 731
BseYI CCCAGC 1 cut(s) 28
BshFI GGCC 3 cut(s) 704, 931, 1126
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 278, 1073
BslI CCNNNNNNNGG 4 cut(s) 75, 101, 145, 330
BsmAI GTCTC 1 cut(s) 854
BsnI GGCC 3 cut(s) 704, 931, 1126
Bso31I GGTCTC 1 cut(s) 854
Bsp1286I GDGCHC 2 cut(s) 50, 137
Bsp143I GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 4 cut(s) 242, 256, 997, 1070
BspANI GGCC 3 cut(s) 704, 931, 1126
BspCNI CTCAG 5 cut(s) 36, 122, 811, 946, 1141
BspLI GGNNCC 4 cut(s) 106, 136, 282, 1167
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 3 cut(s) 166, 834, 994
BspTNI GGTCTC 1 cut(s) 854
BsrBI CCGCTC 1 cut(s) 258
BsrDI GCAATG 3 cut(s) 678, 729, 1059
BsrI ACTGG 4 cut(s) 114, 526, 732, 947
BssECI CCNNGG 2 cut(s) 267, 1161
BssMI GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
Bst2UI CCWGG 2 cut(s) 331, 1163
Bst4CI ACNGT 1 cut(s) 1080
Bst6I CTCTTC 1 cut(s) 94
BstBAI YACGTR 2 cut(s) 577, 657
BstDEI CTNAG 5 cut(s) 44, 109, 798, 954, 1128
BstDSI CCRYGG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 73
BstF5I GGATG 2 cut(s) 394, 907
BstKTI GATC 7 cut(s) 169, 174, 784, 829, 989, 1024, 1105
BstMAI GTCTC 1 cut(s) 854
BstMBI GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 194, 370
BstNI CCWGG 2 cut(s) 331, 1163
BstNSI RCATGY 1 cut(s) 21
BstPI GGTNACC 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 329, 1161
BstSFI CTRYAG 3 cut(s) 717, 837, 1043
BstV1I GCAGC 2 cut(s) 346, 731
BstV2I GAAGAC 2 cut(s) 70, 876
BstX2I RGATCY 1 cut(s) 986
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 986
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 3 cut(s) 704, 931, 1126
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 2 cut(s) 394, 907
BtsIMutI CAGTG 2 cut(s) 739, 951
CaiI CAGNNNCTG 1 cut(s) 953
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 5 cut(s) 117, 149, 425, 654, 744
CviAII CATG 5 cut(s) 18, 434, 641, 1025, 1106
CviQI GTAC 5 cut(s) 117, 149, 425, 654, 744
DdeI CTNAG 5 cut(s) 44, 109, 798, 954, 1128
DpnI GATC 7 cut(s) 168, 173, 783, 828, 988, 1023, 1104
DpnII GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EciI GGCGGA 1 cut(s) 1059
Eco147I AGGCCT 1 cut(s) 1126
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 1158
Eco47I GGWCC 1 cut(s) 290
Eco57I CTGAAG 2 cut(s) 161, 888
Eco81I CCTNAGG 1 cut(s) 1128
Eco91I GGTNACC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 73
EcoO65I GGTNACC 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 329, 1161
EcoRV GATATC 1 cut(s) 1158
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 5 cut(s) 21, 437, 644, 1028, 1109
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FatI CATG 5 cut(s) 17, 433, 640, 1024, 1105
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 1 cut(s) 1102
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 335, 720
FokI GGATG 2 cut(s) 401, 914
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 335, 720
FspBI CTAG 3 cut(s) 120, 561, 608
GluI GCNGC 2 cut(s) 335, 720
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 2 cut(s) 307, 313
HaeIII GGCC 3 cut(s) 704, 931, 1126
HapII CCGG 2 cut(s) 278, 1073
Hin1II CATG 5 cut(s) 21, 437, 644, 1028, 1109
HincII GTYRAC 3 cut(s) 379, 514, 729
HindII GTYRAC 3 cut(s) 379, 514, 729
HinfI GANTC 4 cut(s) 301, 415, 531, 1061
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 2 cut(s) 278, 1073
HphI GGTGA 2 cut(s) 72, 84
Hpy166II GTNNAC 3 cut(s) 379, 514, 729
Hpy188I TCNGA 6 cut(s) 171, 349, 786, 973, 1102, 1178
Hpy188III TCNNGA 8 cut(s) 60, 211, 288, 368, 407, 482, 528, 608
Hpy8I GTNNAC 3 cut(s) 379, 514, 729
HpyAV CCTTC 5 cut(s) 185, 259, 294, 395, 817
HpyCH4III ACNGT 1 cut(s) 1080
HpyCH4IV ACGT 2 cut(s) 576, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 370
HpyF3I CTNAG 5 cut(s) 44, 109, 798, 954, 1128
HpySE526I ACGT 2 cut(s) 576, 656
Hsp92II CATG 5 cut(s) 21, 437, 644, 1028, 1109
Ksp22I TGATCA 1 cut(s) 1102
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
LmnI GCTCC 6 cut(s) 110, 140, 263, 286, 326, 332
Lsp1109I GCAGC 2 cut(s) 346, 731
LweI GCATC 3 cut(s) 496, 777, 923
MaeI CTAG 3 cut(s) 120, 561, 608
MaeII ACGT 2 cut(s) 576, 656
MaeIII GTNAC 3 cut(s) 78, 949, 1009
MalI GATC 7 cut(s) 168, 173, 783, 828, 988, 1023, 1104
MbiI CCGCTC 1 cut(s) 258
MboI GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
MflI RGATCY 1 cut(s) 986
MhlI GDGCHC 2 cut(s) 50, 137
MluCI AATT 3 cut(s) 693, 860, 979
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 3 cut(s) 518, 728, 750
MslI CAYNNNNRTG 2 cut(s) 266, 438
MspI CCGG 2 cut(s) 278, 1073
MspR9I CCNGG 2 cut(s) 331, 1163
MvaI CCWGG 2 cut(s) 331, 1163
MwoI GCNNNNNNNGC 2 cut(s) 194, 370
NdeI CATATG 1 cut(s) 667
NdeII GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
NlaIII CATG 5 cut(s) 21, 437, 644, 1028, 1109
NlaIV GGNNCC 4 cut(s) 106, 136, 282, 1167
NmeAIII GCCGAG 1 cut(s) 831
NmuCI GTSAC 3 cut(s) 78, 949, 1009
NspI RCATGY 1 cut(s) 21
OliI CACNNNNGTG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 1126
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 4 cut(s) 301, 415, 531, 1061
PkrI GCNGC 2 cut(s) 336, 721
Ppu21I YACGTR 2 cut(s) 577, 657
Psp6I CCWGG 2 cut(s) 329, 1161
PspEI GGTNACC 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 2 cut(s) 329, 1161
PspN4I GGNNCC 4 cut(s) 106, 136, 282, 1167
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 1 cut(s) 986
RsaI GTAC 5 cut(s) 118, 150, 426, 655, 745
RsaNI GTAC 5 cut(s) 117, 149, 425, 654, 744
RseI CAYNNNNRTG 2 cut(s) 266, 438
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 518, 728, 750
SatI GCNGC 2 cut(s) 335, 720
Sau3AI GATC 7 cut(s) 166, 171, 781, 826, 986, 1021, 1102
Sau96I GGNCC 1 cut(s) 290
ScaI AGTACT 2 cut(s) 118, 745
ScrFI CCNGG 2 cut(s) 331, 1163
SduI GDGCHC 2 cut(s) 50, 137
SfaNI GCATC 3 cut(s) 496, 777, 923
SfcI CTRYAG 3 cut(s) 717, 837, 1043
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 2 cut(s) 266, 438
SmlI CTYRAG 2 cut(s) 368, 1120
SmoI CTYRAG 2 cut(s) 368, 1120
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 3 cut(s) 693, 860, 979
SseBI AGGCCT 1 cut(s) 1126
SsiI CCGC 4 cut(s) 242, 256, 997, 1070
SspMI CTAG 3 cut(s) 120, 561, 608
StuI AGGCCT 1 cut(s) 1126
StyD4I CCNGG 2 cut(s) 329, 1161
TaaI ACNGT 1 cut(s) 1080
TaiI ACGT 2 cut(s) 579, 659
TaqI TCGA 5 cut(s) 192, 513, 543, 776, 1087
TasI AATT 3 cut(s) 693, 860, 979
TatI WGTACW 2 cut(s) 116, 743
TfiI GAWTC 4 cut(s) 301, 415, 531, 1061
Tru1I TTAA 3 cut(s) 518, 728, 750
Tru9I TTAA 3 cut(s) 518, 728, 750
TscAI CASTG 2 cut(s) 739, 958
TseFI GTSAC 3 cut(s) 78, 949, 1009
TseI GCWGC 2 cut(s) 334, 719
Tsp45I GTSAC 3 cut(s) 78, 949, 1009
TspDTI ATGAA 4 cut(s) 225, 422, 700, 994
TspGWI ACGGA 3 cut(s) 256, 675, 999
TspRI CASTG 2 cut(s) 739, 958
VpaK11BI GGWCC 1 cut(s) 290
XagI CCTNNNNNAGG 1 cut(s) 73
XapI RAATTY 1 cut(s) 860
XbaI TCTAGA 1 cut(s) 607
XceI RCATGY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 2 cut(s) 227, 337
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 3 cut(s) 120, 561, 608
ZrmI AGTACT 2 cut(s) 118, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.