Rroxscaffold_5G00379240

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012513
Physical Location & Seq
Reverse (-)
59677275 .. 59682906
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379240.1

Sequence Viewer

Length: 1152 bp
ATGGGAAACTCAATGGCATGCTTCTCCCCTGCGCACATACCAAAGAAGCTGAGCACCAACTTGTCAAGGAAGCCCTCCAAGAGGTCACCCAACTCTTCACCTTTCTTTGGCTCCTCGGCTAGTACTAGAAGAACCAAGGGTTCTCCCAAGAAGACGTCACAGACCCTTGCTGATGATTTGATGCTTCAACAACAAGCTATAGAGGCGGTTCTGTTGTTCCAGAACCACCAGAAGAACAATGGATCGCAACCCTTCAACCGGTCCACCTCTGTGGTGTTTCCGGCTCCTGGACCAAAGAAGAATCAGGCCTTCACGAAAAGCTCCAGCTCTAGGCAGCCTTTGGGTTCTGAGACTCTCTTTCAGCCTCTTGAGCTTGTCGACAAGCAGCAGGATGTAAAGATTGATGGTCTGGAAACAAATCATTTTGTACTTGTTCACGGAGGTGGCTTTGGTGCTTGGTGCTGGTATAAGACAATGACACTTCTAGAAGAAAGTGGGTTCAAGGTTGATGCAGTTGACTTAACTGGTTCCGGAATCCACTCATTTGATACAAACAGCATTACAAGTCTTGCACAATATGTGAAGCCACTTACTGAAATCCTTGAGAATCTAGAGGAGGGGAAAAAGGTCATTTTGGTTGGACATGATTTTGGTGGTACATGTATCTCATATGCAATGGAGTTGTTTCCATCTAAAATTGCAAAGGCCATCTTCATATCTGCAGCAATGTTAACTAGTGGGCAGAGTACTCTTAATCTGTTTGCTCAACAGACAGGCTCGAATGATCTGATGCGTCAGGCTCAGATATTCTTGTATGCAAATGGGAAGGATCAGCCTCCTACTGCTATTGGTCTCGGCAAAAAATTTACCGAAGACTTATTGTTCAATCAAAGTCCAGCAAAGGATGTTGCATTGGCATCAGTATCAATGAGGCCAATCCCTTTTGCACCAGTAACTGAGAAGCTCTCTCTTTCTGACAAGAATTACGGATCTGTCAGGCGGTTTTTCATAGTCACTCAAGAAGATCATGCCATCAGCGTTCCACTACAGGAGGCCATGCTAGAATCAAACCCGCCAGAACAGTTTTATCGACTAAAAGGCTCTGATCATGCACCTTTTTCTCAAGGCCTCAGGCTTTGCATAGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

383

Amino Acids

41.92

Weight (kDa)

8.99

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 140 - 253 5.7e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 142 - 374 1.7e-17 Alpha/beta hydrolase family
Hydrolase_4 PF12146 142 - 253 1.2e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 158
Acc16I TGCGCA 1 cut(s) 33
AccI GTMKAC 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 530
AciI CCGC 3 cut(s) 206, 1000, 1073
AclWI GGATC 3 cut(s) 250, 837, 997
AcsI RAATTY 1 cut(s) 863
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 4 cut(s) 124, 429, 658, 748
AfiI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
AflIII ACRYGT 1 cut(s) 659
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 4 cut(s) 188, 256, 502, 886
AhlI ACTAGT 1 cut(s) 734
AjnI CCWGG 1 cut(s) 286
AleI CACNNNNGTG 2 cut(s) 269, 441
AluBI AGCT 6 cut(s) 49, 197, 321, 327, 373, 964
AluI AGCT 6 cut(s) 49, 197, 321, 327, 373, 964
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 344, 857
AlwI GGATC 3 cut(s) 250, 837, 997
AlwNI CAGNNNCTG 1 cut(s) 956
Aor13HI TCCGGA 1 cut(s) 530
AoxI GGCC 5 cut(s) 306, 705, 932, 1053, 1126
ApeKI GCWGC 3 cut(s) 334, 385, 722
ApoI RAATTY 1 cut(s) 863
AsiGI ACCGGT 1 cut(s) 258
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 261, 290
AsuHPI GGTGA 2 cut(s) 78, 90
AvaII GGWCC 2 cut(s) 261, 290
AxyI CCTNAGG 1 cut(s) 1130
BbsI GAAGAC 2 cut(s) 158, 879
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 346, 397, 734
BccI CCATC 4 cut(s) 398, 697, 716, 1040
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 1105
BcoDI GTCTC 2 cut(s) 344, 857
BcuI ACTAGT 1 cut(s) 734
BfaI CTAG 7 cut(s) 120, 126, 330, 485, 611, 735, 1061
BfmI CTRYAG 3 cut(s) 198, 720, 1046
BisI GCNGC 3 cut(s) 335, 386, 723
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 3 cut(s) 336, 387, 724
BmcAI AGTACT 2 cut(s) 124, 748
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 2 cut(s) 261, 290
BmgT120I GGNCC 2 cut(s) 261, 290
BmiI GGNNCC 3 cut(s) 112, 285, 529
BmrFI CCNGG 1 cut(s) 288
BmsI GCATC 4 cut(s) 171, 499, 780, 926
BpiI GAAGAC 2 cut(s) 158, 879
BplI GAGNNNNNCTC 2 cut(s) 950, 982
BpmI CTGGAG 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 4 cut(s) 389, 623, 1002, 1107
BsaHI GRCGYC 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 2 cut(s) 114, 135
BsaWI WCCGGW 2 cut(s) 258, 530
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 2 cut(s) 529, 950
Bse21I CCTNAGG 1 cut(s) 1130
Bse3DI GCAATG 2 cut(s) 681, 732
BseAI TCCGGA 1 cut(s) 530
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 114, 135
BseGI GGATG 2 cut(s) 397, 910
BseLI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
BseMI GCAATG 2 cut(s) 681, 732
BseMII CTCAG 5 cut(s) 41, 339, 815, 948, 1144
BseNI ACTGG 2 cut(s) 529, 950
BseRI GAGGAG 2 cut(s) 103, 629
BseXI GCAGC 3 cut(s) 346, 397, 734
BshFI GGCC 5 cut(s) 308, 707, 934, 1055, 1128
BshTI ACCGGT 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 259, 281, 531
BslI CCNNNNNNNGG 5 cut(s) 81, 107, 258, 287, 330
BsmAI GTCTC 2 cut(s) 344, 857
BsnI GGCC 5 cut(s) 308, 707, 934, 1055, 1128
Bso31I GGTCTC 1 cut(s) 857
Bsp1286I GDGCHC 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 530
Bsp143I GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 3 cut(s) 206, 1000, 1073
BspANI GGCC 5 cut(s) 308, 707, 934, 1055, 1128
BspCNI CTCAG 5 cut(s) 42, 340, 814, 949, 1143
BspEI TCCGGA 1 cut(s) 530
BspLI GGNNCC 3 cut(s) 112, 285, 529
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 3 cut(s) 250, 837, 997
BspTNI GGTCTC 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 681, 732
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 2 cut(s) 529, 950
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 2 cut(s) 114, 135
BssMI GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 1083
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 5 cut(s) 50, 348, 801, 957, 1130
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 397, 910
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 6 cut(s) 245, 787, 832, 992, 1027, 1108
BstMAI GTCTC 2 cut(s) 344, 857
BstMBI GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 203, 370
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 2 cut(s) 21, 663
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 3 cut(s) 198, 720, 1046
BstV1I GCAGC 3 cut(s) 346, 397, 734
BstV2I GAAGAC 2 cut(s) 158, 879
BstX2I RGATCY 1 cut(s) 989
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 1 cut(s) 989
Bsu36I CCTNAGG 1 cut(s) 1130
BsuRI GGCC 5 cut(s) 308, 707, 934, 1055, 1128
BtsCI GGATG 2 cut(s) 397, 910
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 956
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 261, 290
CseI GACGC 1 cut(s) 782
Csp6I GTAC 4 cut(s) 123, 428, 657, 747
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 6 cut(s) 18, 644, 660, 1028, 1057, 1109
CviQI GTAC 4 cut(s) 123, 428, 657, 747
DdeI CTNAG 5 cut(s) 50, 348, 801, 957, 1130
DpnI GATC 6 cut(s) 244, 786, 831, 991, 1026, 1107
DpnII GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 2 cut(s) 308, 1128
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 2 cut(s) 261, 290
Eco81I CCTNAGG 1 cut(s) 1130
Eco91I GGTNACC 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 6 cut(s) 21, 647, 663, 1031, 1060, 1112
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FatI CATG 6 cut(s) 17, 643, 659, 1027, 1056, 1108
FauI CCCGC 1 cut(s) 1080
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 3 cut(s) 335, 386, 723
FokI GGATG 2 cut(s) 404, 917
Fsp4HI GCNGC 3 cut(s) 335, 386, 723
FspBI CTAG 7 cut(s) 120, 126, 330, 485, 611, 735, 1061
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 3 cut(s) 335, 386, 723
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 5 cut(s) 308, 707, 934, 1055, 1128
HapII CCGG 3 cut(s) 259, 281, 531
HgaI GACGC 1 cut(s) 782
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 6 cut(s) 21, 647, 663, 1031, 1060, 1112
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 3 cut(s) 379, 517, 732
HindII GTYRAC 3 cut(s) 379, 517, 732
HinfI GANTC 5 cut(s) 301, 352, 534, 607, 1064
HpaI GTTAAC 1 cut(s) 732
HpaII CCGG 3 cut(s) 259, 281, 531
HphI GGTGA 2 cut(s) 78, 90
Hpy166II GTNNAC 5 cut(s) 264, 379, 436, 517, 732
Hpy188I TCNGA 5 cut(s) 349, 789, 804, 976, 1105
Hpy188III TCNNGA 8 cut(s) 220, 313, 368, 410, 485, 531, 611, 1019
Hpy8I GTNNAC 5 cut(s) 264, 379, 436, 517, 732
HpyAV CCTTC 3 cut(s) 262, 319, 820
HpyCH4III ACNGT 1 cut(s) 1083
HpyCH4IV ACGT 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 370
HpyF3I CTNAG 5 cut(s) 50, 348, 801, 957, 1130
HpySE526I ACGT 1 cut(s) 155
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 6 cut(s) 21, 647, 663, 1031, 1060, 1112
HspAI GCGC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 530
Ksp22I TGATCA 1 cut(s) 1105
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
LmnI GCTCC 3 cut(s) 116, 289, 326
Lsp1109I GCAGC 3 cut(s) 346, 397, 734
LweI GCATC 4 cut(s) 171, 499, 780, 926
MaeI CTAG 7 cut(s) 120, 126, 330, 485, 611, 735, 1061
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 4 cut(s) 84, 156, 952, 1012
MalI GATC 6 cut(s) 244, 786, 831, 991, 1026, 1107
MboI GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
MboII GAAGA 9 cut(s) 87, 141, 163, 244, 310, 500, 703, 884, 1034
MflI RGATCY 1 cut(s) 989
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 696, 863, 982
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 619
MroI TCCGGA 1 cut(s) 530
MseI TTAA 3 cut(s) 521, 731, 753
MslI CAYNNNNRTG 2 cut(s) 269, 441
MspI CCGG 3 cut(s) 259, 281, 531
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 203, 370
NdeI CATATG 1 cut(s) 670
NdeII GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
NlaIII CATG 6 cut(s) 21, 647, 663, 1031, 1060, 1112
NlaIV GGNNCC 3 cut(s) 112, 285, 529
NmeAIII GCCGAG 2 cut(s) 95, 834
NmuCI GTSAC 3 cut(s) 84, 156, 1012
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 2 cut(s) 21, 663
OliI CACNNNNGTG 2 cut(s) 269, 441
PaeI GCATGC 1 cut(s) 21
PceI AGGCCT 2 cut(s) 308, 1128
PciI ACATGT 1 cut(s) 659
PfeI GAWTC 4 cut(s) 301, 534, 607, 1064
PfoI TCCNGGA 1 cut(s) 286
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 3 cut(s) 336, 387, 724
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PscI ACATGT 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 286
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 3 cut(s) 112, 285, 529
PspPI GGNCC 2 cut(s) 261, 290
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 1 cut(s) 989
RsaI GTAC 4 cut(s) 124, 429, 658, 748
RsaNI GTAC 4 cut(s) 123, 428, 657, 747
RseI CAYNNNNRTG 2 cut(s) 269, 441
SalI GTCGAC 1 cut(s) 377
SaqAI TTAA 3 cut(s) 521, 731, 753
SatI GCNGC 3 cut(s) 335, 386, 723
Sau3AI GATC 6 cut(s) 242, 784, 829, 989, 1024, 1105
Sau96I GGNCC 2 cut(s) 261, 290
ScaI AGTACT 2 cut(s) 124, 748
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 4 cut(s) 171, 499, 780, 926
SfcI CTRYAG 3 cut(s) 198, 720, 1046
SinI GGWCC 2 cut(s) 261, 290
SmiMI CAYNNNNRTG 2 cut(s) 269, 441
SmlI CTYRAG 4 cut(s) 368, 602, 1017, 1122
SmoI CTYRAG 4 cut(s) 368, 602, 1017, 1122
SpeI ACTAGT 1 cut(s) 734
SphI GCATGC 1 cut(s) 21
Sse9I AATT 3 cut(s) 696, 863, 982
SseBI AGGCCT 2 cut(s) 308, 1128
SsiI CCGC 3 cut(s) 206, 1000, 1073
SspMI CTAG 7 cut(s) 120, 126, 330, 485, 611, 735, 1061
StuI AGGCCT 2 cut(s) 308, 1128
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 1083
TaiI ACGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 378, 779, 1090
TasI AATT 3 cut(s) 696, 863, 982
TatI WGTACW 3 cut(s) 122, 427, 746
TfiI GAWTC 4 cut(s) 301, 534, 607, 1064
Tru1I TTAA 3 cut(s) 521, 731, 753
Tru9I TTAA 3 cut(s) 521, 731, 753
TseFI GTSAC 3 cut(s) 84, 156, 1012
TseI GCWGC 3 cut(s) 334, 385, 722
Tsp45I GTSAC 3 cut(s) 84, 156, 1012
TspDTI ATGAA 2 cut(s) 703, 997
TspGWI ACGGA 2 cut(s) 453, 1002
VpaK11BI GGWCC 2 cut(s) 261, 290
XagI CCTNNNNNAGG 1 cut(s) 79
XapI RAATTY 1 cut(s) 863
XbaI TCTAGA 2 cut(s) 484, 610
XceI RCATGY 2 cut(s) 21, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 378
XspI CTAG 7 cut(s) 120, 126, 330, 485, 611, 735, 1061
ZraI GACGTC 1 cut(s) 156
ZrmI AGTACT 2 cut(s) 124, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.