RLG00000001449

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012513
Physical Location & Seq
Reverse (-)
15713085 .. 15715237
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001449

Sequence Viewer

Length: 1047 bp
ATGCTTCAACAACAAGCTATAGAGGCGGTTCTGTTGTTCCAGAACCACCAGAAGAACAATGGATCGCAACCCTTCAACCGGTCCACCTCTGTGGTGTTTCCGGCTCCTGGACCAAAGAAGAATCAGGCCTTTATGAAAAGCTCCAGCTCTAGGCAGCCTTTGGGTTCTGAGACTCTCTTTCATTCTCTTGAGCTTGTTGACAAGCAGGATGTAAAGATTGATGGTCTGGAAACAAATCATTTTGTACTTGTTCATGATGGAGGTGGCTTTGGTGCTTGGTGCTGGTATAAAACAATGACACTTCTAGAAGAAAGTGGGTTCAAGGTTGATGCAGTTGACTTAACTGGTTCCGGAATCCACTCATTCGATACAAACAGCATTACAAGTCTTGCACAATATGTGAAGCCACTTACTGAAATCCTTGAGAATCTGGAGGAGGGGAAAAAGGTTTTGATTGCACACCGTGTTAGTCTAAGCTTCATTACAATTATAAAATGCAGGTATGATTTTGGTGGTACATGTATCTCATATGCAATGGAGTTGTTTCCATCTAAAATTGCAAAGGCCATCTTCATATCTGCAGCAATGTTAACTAGTGGGCAGAGTACTCTTAATCTGTTTGCTCAACAGACAGGCTCGAACGATCTGATGCGTCAGGCTCAGATATTCTTGTATGCAAATGGGAAGGATCAACCTCCTACTGCTATTGGTCTCGGCAAAAAATTTACCGAAGACTTATTGTTCAATCAAAGTCCAGCAAAGGATGTTGCATTGGCATCAGTATCAATGAGGCCAATCCCTTTTGCACCAGTCACAGAGAAGCTCTCTCTTTCTGATAAGAATTACGGATCTGTCAGGCGGTTTTTCATAGTCACTCAAGAAGATCATGCCATAAGCGTTCCACTACAGGAGGCAATGCTAGAATCAAACCCGCCCGAACAGTTTTATCGACTAAAAGGCTCTGATCATGCACCTTTTTTCTCAAGGCCTCAGGCTTTGCATAGGATATTAGTAGAGATATCCCAGGTTCCACCTAATCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

349

Amino Acids

38.52

Weight (kDa)

6.76

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26360 AT1G69240
fragaria_vesca FvH4_4g29850
malus_domestica MD13G1061500.v1.1
prunus_persica Prupe.1G288600_v2.0.a1
pyrus_communis pycom13g05410
rosa_chinensis RchiOBHm_Chr4g0438101
rosa_laevigata RLG00000001449 RLG00000006388
rosa_multiflora Rmu_sc0027297.1_g000003
rosa_roxburghii Rroxscaffold_5G00379240
rosa_rugosa Rorug04G0307000 Rorug04G0307100
rosa_samantha Rh4AG358500 Rh4BG369400 Rh4CG383500 Rh4DG364300
rosa_wichuraiana Rw0G006300 Rw0G016240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 3 cut(s) 26, 859, 932
AclWI GGATC 3 cut(s) 70, 696, 856
AcsI RAATTY 1 cut(s) 722
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 3 cut(s) 246, 517, 607
AfiI CCNNNNNNNGG 3 cut(s) 78, 107, 150
AflIII ACRYGT 1 cut(s) 518
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 4 cut(s) 8, 76, 322, 745
AhlI ACTAGT 1 cut(s) 593
AjnI CCWGG 2 cut(s) 106, 1023
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 6 cut(s) 17, 141, 147, 193, 477, 823
AluI AGCT 6 cut(s) 17, 141, 147, 193, 477, 823
Alw26I GTCTC 2 cut(s) 164, 716
AlwI GGATC 3 cut(s) 70, 696, 856
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 4 cut(s) 126, 564, 791, 986
ApeKI GCWGC 2 cut(s) 154, 581
ApoI RAATTY 1 cut(s) 722
AsiGI ACCGGT 1 cut(s) 78
AspS9I GGNCC 2 cut(s) 81, 110
AvaII GGWCC 2 cut(s) 81, 110
AxyI CCTNAGG 1 cut(s) 990
BbsI GAAGAC 1 cut(s) 738
BbvI GCAGC 2 cut(s) 166, 593
BccI CCATC 4 cut(s) 215, 251, 556, 575
BciT130I CCWGG 2 cut(s) 108, 1025
BclI TGATCA 1 cut(s) 964
BcoDI GTCTC 2 cut(s) 164, 716
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 4 cut(s) 150, 305, 594, 920
BfmI CTRYAG 3 cut(s) 18, 579, 905
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 2 cut(s) 155, 582
BlsI GCNGC 2 cut(s) 156, 583
BmcAI AGTACT 1 cut(s) 607
Bme1390I CCNGG 2 cut(s) 108, 1025
Bme18I GGWCC 2 cut(s) 81, 110
BmgT120I GGNCC 2 cut(s) 81, 110
BmiI GGNNCC 3 cut(s) 105, 349, 1029
BmrFI CCNGG 2 cut(s) 108, 1025
BmsI GCATC 3 cut(s) 319, 639, 785
BpiI GAAGAC 1 cut(s) 738
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 2 cut(s) 127, 452
BpuEI CTTGAG 4 cut(s) 209, 443, 861, 967
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 1 cut(s) 1023
BsaWI WCCGGW 2 cut(s) 78, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 107, 150
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 349, 809
Bse21I CCTNAGG 1 cut(s) 990
Bse3DI GCAATG 3 cut(s) 540, 591, 921
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 2 cut(s) 108, 1025
BseDI CCNNGG 1 cut(s) 1023
BseGI GGATG 2 cut(s) 214, 769
BseLI CCNNNNNNNGG 3 cut(s) 78, 107, 150
BseMI GCAATG 3 cut(s) 540, 591, 921
BseMII CTCAG 4 cut(s) 159, 674, 1004, 1031
BseNI ACTGG 2 cut(s) 349, 809
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 166, 593
BshFI GGCC 4 cut(s) 128, 566, 793, 988
BshTI ACCGGT 1 cut(s) 78
BsiSI CCGG 3 cut(s) 79, 101, 351
BslI CCNNNNNNNGG 3 cut(s) 78, 107, 150
BsmAI GTCTC 2 cut(s) 164, 716
BsnI GGCC 4 cut(s) 128, 566, 793, 988
Bso31I GGTCTC 1 cut(s) 716
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 6 cut(s) 62, 643, 688, 848, 883, 964
BspACI CCGC 3 cut(s) 26, 859, 932
BspANI GGCC 4 cut(s) 128, 566, 793, 988
BspCNI CTCAG 4 cut(s) 160, 673, 1003, 1032
BspEI TCCGGA 1 cut(s) 350
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 105, 349, 1029
BspMAI CTGCAG 1 cut(s) 583
BspMI ACCTGC 1 cut(s) 489
BspPI GGATC 3 cut(s) 70, 696, 856
BspTNI GGTCTC 1 cut(s) 716
BsrDI GCAATG 3 cut(s) 540, 591, 921
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 2 cut(s) 349, 809
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 1023
BssMI GATC 6 cut(s) 62, 643, 688, 848, 883, 964
Bst2UI CCWGG 2 cut(s) 108, 1025
Bst4CI ACNGT 2 cut(s) 464, 942
BstDEI CTNAG 5 cut(s) 168, 473, 660, 990, 1040
BstF5I GGATG 2 cut(s) 214, 769
BstKTI GATC 6 cut(s) 65, 646, 691, 851, 886, 967
BstMAI GTCTC 2 cut(s) 164, 716
BstMBI GATC 6 cut(s) 62, 643, 688, 848, 883, 964
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 108, 1025
BstNSI RCATGY 1 cut(s) 522
BstSCI CCNGG 2 cut(s) 106, 1023
BstSFI CTRYAG 3 cut(s) 18, 579, 905
BstV1I GCAGC 2 cut(s) 166, 593
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 1 cut(s) 848
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 848
Bsu36I CCTNAGG 1 cut(s) 990
BsuRI GGCC 4 cut(s) 128, 566, 793, 988
BtsCI GGATG 2 cut(s) 214, 769
BveI ACCTGC 1 cut(s) 489
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 2 cut(s) 81, 110
CseI GACGC 1 cut(s) 641
Csp6I GTAC 3 cut(s) 245, 516, 606
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 4 cut(s) 254, 519, 887, 968
CviQI GTAC 3 cut(s) 245, 516, 606
DdeI CTNAG 5 cut(s) 168, 473, 660, 990, 1040
DpnI GATC 6 cut(s) 64, 645, 690, 850, 885, 966
DpnII GATC 6 cut(s) 62, 643, 688, 848, 883, 964
DraIII CACNNNGTG 1 cut(s) 464
Eco147I AGGCCT 2 cut(s) 128, 988
Eco31I GGTCTC 1 cut(s) 716
Eco32I GATATC 1 cut(s) 1020
Eco47I GGWCC 2 cut(s) 81, 110
Eco81I CCTNAGG 1 cut(s) 990
EcoRII CCWGG 2 cut(s) 106, 1023
EcoRV GATATC 1 cut(s) 1020
FaeI CATG 4 cut(s) 257, 522, 890, 971
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FatI CATG 4 cut(s) 253, 518, 886, 967
FauI CCCGC 1 cut(s) 939
FauNDI CATATG 1 cut(s) 529
FbaI TGATCA 1 cut(s) 964
Fnu4HI GCNGC 2 cut(s) 155, 582
FokI GGATG 2 cut(s) 221, 776
Fsp4HI GCNGC 2 cut(s) 155, 582
FspBI CTAG 4 cut(s) 150, 305, 594, 920
GluI GCNGC 2 cut(s) 155, 582
GsuI CTGGAG 2 cut(s) 127, 452
HaeIII GGCC 4 cut(s) 128, 566, 793, 988
HapII CCGG 3 cut(s) 79, 101, 351
HgaI GACGC 1 cut(s) 641
Hin1II CATG 4 cut(s) 257, 522, 890, 971
HincII GTYRAC 3 cut(s) 199, 337, 591
HindII GTYRAC 3 cut(s) 199, 337, 591
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 5 cut(s) 121, 172, 354, 427, 923
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 3 cut(s) 79, 101, 351
Hpy166II GTNNAC 4 cut(s) 84, 199, 337, 591
Hpy188I TCNGA 6 cut(s) 169, 648, 663, 835, 964, 1041
Hpy188III TCNNGA 8 cut(s) 40, 188, 227, 254, 305, 351, 431, 878
Hpy8I GTNNAC 4 cut(s) 84, 199, 337, 591
HpyAV CCTTC 2 cut(s) 82, 679
HpyCH4III ACNGT 2 cut(s) 464, 942
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 5 cut(s) 168, 473, 660, 990, 1040
Hsp92II CATG 4 cut(s) 257, 522, 890, 971
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 964
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 6 cut(s) 62, 643, 688, 848, 883, 964
LmnI GCTCC 2 cut(s) 109, 146
Lsp1109I GCAGC 2 cut(s) 166, 593
LweI GCATC 3 cut(s) 319, 639, 785
MaeI CTAG 4 cut(s) 150, 305, 594, 920
MaeIII GTNAC 2 cut(s) 811, 871
MalI GATC 6 cut(s) 64, 645, 690, 850, 885, 966
MboI GATC 6 cut(s) 62, 643, 688, 848, 883, 964
MboII GAAGA 6 cut(s) 64, 130, 320, 562, 743, 893
MflI RGATCY 1 cut(s) 848
MluCI AATT 4 cut(s) 486, 555, 722, 841
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 9 cut(s) 16, 97, 254, 427, 430, 705, 783, 904, 999
MroI TCCGGA 1 cut(s) 350
MseI TTAA 3 cut(s) 341, 590, 612
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 3 cut(s) 79, 101, 351
MspR9I CCNGG 2 cut(s) 108, 1025
MvaI CCWGG 2 cut(s) 108, 1025
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeI CATATG 1 cut(s) 529
NdeII GATC 6 cut(s) 62, 643, 688, 848, 883, 964
NlaIII CATG 4 cut(s) 257, 522, 890, 971
NlaIV GGNNCC 3 cut(s) 105, 349, 1029
NmeAIII GCCGAG 1 cut(s) 693
NmuCI GTSAC 2 cut(s) 811, 871
NspI RCATGY 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 253
PceI AGGCCT 2 cut(s) 128, 988
PciI ACATGT 1 cut(s) 518
PfeI GAWTC 4 cut(s) 121, 354, 427, 923
PfoI TCCNGGA 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 2 cut(s) 156, 583
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PscI ACATGT 1 cut(s) 518
PsiI TTATAA 1 cut(s) 491
Psp6I CCWGG 2 cut(s) 106, 1023
PspGI CCWGG 2 cut(s) 106, 1023
PspN4I GGNNCC 3 cut(s) 105, 349, 1029
PspPI GGNCC 2 cut(s) 81, 110
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 1 cut(s) 848
RsaI GTAC 3 cut(s) 246, 517, 607
RsaNI GTAC 3 cut(s) 245, 516, 606
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 3 cut(s) 341, 590, 612
SatI GCNGC 2 cut(s) 155, 582
Sau3AI GATC 6 cut(s) 62, 643, 688, 848, 883, 964
Sau96I GGNCC 2 cut(s) 81, 110
ScaI AGTACT 1 cut(s) 607
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 108, 1025
SfaNI GCATC 3 cut(s) 319, 639, 785
SfcI CTRYAG 3 cut(s) 18, 579, 905
SinI GGWCC 2 cut(s) 81, 110
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 4 cut(s) 188, 422, 876, 982
SmoI CTYRAG 4 cut(s) 188, 422, 876, 982
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 4 cut(s) 486, 555, 722, 841
SseBI AGGCCT 2 cut(s) 128, 988
SsiI CCGC 3 cut(s) 26, 859, 932
SspMI CTAG 4 cut(s) 150, 305, 594, 920
StuI AGGCCT 2 cut(s) 128, 988
StyD4I CCNGG 2 cut(s) 106, 1023
TaaI ACNGT 2 cut(s) 464, 942
TaqI TCGA 3 cut(s) 366, 638, 949
TasI AATT 4 cut(s) 486, 555, 722, 841
TatI WGTACW 2 cut(s) 244, 605
TfiI GAWTC 4 cut(s) 121, 354, 427, 923
Tru1I TTAA 3 cut(s) 341, 590, 612
Tru9I TTAA 3 cut(s) 341, 590, 612
TseFI GTSAC 2 cut(s) 811, 871
TseI GCWGC 2 cut(s) 154, 581
Tsp45I GTSAC 2 cut(s) 811, 871
TspDTI ATGAA 6 cut(s) 149, 170, 242, 469, 562, 856
TspGWI ACGGA 1 cut(s) 861
VpaK11BI GGWCC 2 cut(s) 81, 110
XapI RAATTY 1 cut(s) 722
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 1 cut(s) 522
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XspI CTAG 4 cut(s) 150, 305, 594, 920
ZrmI AGTACT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.