AT1G69500

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011009
Physical Location & Seq
Forward (+)
26123940 .. 26126057
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69500.1

Sequence Viewer

Length: 1575 bp
ATGTCGTTGTGTTTGGTTATAGCTTGTATGGTAACATCATGGATCTTCTTACACCGATGGGGACAAAGAAACAAGAGTGGTCCAAAGACTTGGCCTTTGGTTGGAGCAGCGATCGAACAGTTAACTAATTTTGATCGAATGCATGATTGGCTCGTTGAGTATCTTTATAACTCAAGAACAGTAGTGGTTCCAATGCCATTCACTACTTATACATACATAGCTGATCCCATCAACGTTGAATATGTCCTGAAAACAAACTTCTCCAATTACCCCAAGGGAGAGACTTATCATTCTTATATGGAAGTATTGTTGGGAGATGGGATTTTCAATTCTGATGGAGAGCTTTGGAGGAAACAGAGAAAAACCGCGAGTTTCGAATTTGCTTCCAAGAATCTTAGAGATTTTAGTACTGTAGTGTTTAAAGAGTATAGTCTTAAACTCTTCACTATCCTTTCCCAAGCATCTTTCAAAGAGCAACAAGTAGATATGCAGGAATTGTTGATGAGAATGACTTTGGATTCAATATGTAAAGTGGGATTTGGTGTGGAGATAGGGACATTGGCTCCAGAGTTACCAGAGAATCACTTTGCTAAGGCTTTTGATACCGCAAATATAATCGTAACACTTCGTTTCATAGATCCTCTTTGGAAGATGAAAAAGTTCCTTAACATAGGCTCTGAGGCATTGCTTGGTAAAAGCATAAAAGTAGTCAATGATTTCACCTATTCAGTCATAAGAAGAAGGAAAGCAGAGTTATTAGAGGCACAAATATCTCCTACCAACAACAACAACAACAACAACAACAAGGTGAAGCACGATATACTCTCGAGGTTTATCGAGATCAGTGACGATCCAGATAGCAAAGAAACAGAGAAAAGCCTAAGAGATATTGTCCTCAACTTTGTAATAGCTGGAAGAGATACAACAGCAACAACTCTCACTTGGGCTATATACATGATAATGATGAATGAAAATGTTGCTGAGAAACTATACTCAGAGCTACAAGAACTCGAAAAAGAAAGCGCGGAAGCGACTAATACATCGTTGCATCAATACGATACAGAGGATTTCAATTCCTTCAACGAGAAGGTAACAGAATTCGCAGGACTATTGAACTACGATTCCTTAGGAAAACTTCACTACTTACATGCTGTGATCACAGAAACACTTCGTCTCTACCCTGCCGTTCCTCAGGATCCGAAAGGAGTATTAGAAGATGATATGTTACCAAATGGGACAAAAGTAAAAGCTGGAGGGATGGTCACATATGTACCTTACTCAATGGGTCGTATGGAATACAATTGGGGATCAGATGCAGCATTGTTTAAACCCGAAAGATGGCTTAAAGATGGAGTCTTTCAAAATGCATCACCATTCAAGTTCACAGCTTTTCAGGCTGGACCAAGGATATGTTTGGGAAAGGATTCAGCTTATCTGCAAATGAAGATGGCAATGGCAATACTTTGCAGATTCTATAAGTTTCATTTGGTGCCAAATCATCCTGTCAAGTATCGGATGATGACGATCTTATCCATGGCTCATGGCTTGAAGGTTACTGTATCCAGACGTTCATAG

Protein Analysis

524

Amino Acids

60.1

Weight (kDa)

7.63

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 27 - 512 1.1e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69500
fragaria_vesca FvH4_4g31080 FvH4_4g31080
malus_domestica MD13G1069300.v1.1 MD16G1069600.v1.1
prunus_persica Prupe.1G277400_v2.0.a1
pyrus_communis pycom13g06210 pycom13g06220
rosa_chinensis RchiOBHm_Chr4g0439461
rosa_laevigata RLG00000006269
rosa_multiflora Rmu_sc0007276.1_g000005
rosa_roxburghii Rroxscaffold_5G00380380
rosa_rugosa Rorug04G0318500
rosa_samantha Rh4AG369800 Rh4BG381600 Rh4BG381700 Rh4CG396300 Rh4DG376200 Rh4DG376300
rosa_wichuraiana Rw4G031530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 1489
AccII CGCG 2 cut(s) 368, 1025
AciI CCGC 3 cut(s) 366, 606, 1025
AclI AACGTT 1 cut(s) 234
AclWI GGATC 7 cut(s) 50, 218, 632, 845, 1190, 1203, 1315
AcsI RAATTY 2 cut(s) 377, 1097
AfaI GTAC 2 cut(s) 409, 1272
AfiI CCNNNNNNNGG 2 cut(s) 101, 1338
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AloI GAACNNNNNNTCC 2 cut(s) 1106, 1138
AluBI AGCT 8 cut(s) 23, 221, 343, 911, 1000, 1250, 1388, 1430
AluI AGCT 8 cut(s) 23, 221, 343, 911, 1000, 1250, 1388, 1430
Alw26I GTCTC 2 cut(s) 275, 1178
AlwI GGATC 7 cut(s) 50, 218, 632, 845, 1190, 1203, 1315
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 107, 1316
ApoI RAATTY 2 cut(s) 377, 1097
ArsI GACNNNNNNTTYG 2 cut(s) 79, 111
Asp700I GAANNNNTTC 3 cut(s) 659, 1167, 1423
AspLEI GCGC 1 cut(s) 1025
AspS9I GGNCC 2 cut(s) 80, 1400
AsuHPI GGTGA 3 cut(s) 712, 820, 1362
AsuII TTCGAA 1 cut(s) 375
AvaI CYCGRG 1 cut(s) 826
AvaII GGWCC 2 cut(s) 80, 1400
AxyI CCTNAGG 2 cut(s) 1126, 1191
BaeI ACNNNNGTAYC 2 cut(s) 1254, 1287
BamHI GGATCC 1 cut(s) 1195
BanI GGYRCC 1 cut(s) 1489
BbvI GCAGC 2 cut(s) 119, 1328
BccI CCATC 8 cut(s) 51, 236, 311, 329, 1252, 1332, 1343, 1441
BceAI ACGGC 1 cut(s) 1169
BciVI GTATCC 1 cut(s) 1570
BclI TGATCA 1 cut(s) 1155
BcoDI GTCTC 2 cut(s) 275, 1178
BfmI CTRYAG 1 cut(s) 411
BfuI GTATCC 1 cut(s) 1570
BisI GCNGC 2 cut(s) 108, 1317
BlsI GCNGC 2 cut(s) 109, 1318
BmcAI AGTACT 1 cut(s) 409
Bme18I GGWCC 2 cut(s) 80, 1400
BmeT110I CYCGRG 1 cut(s) 826
BmgT120I GGNCC 2 cut(s) 80, 1400
BmiI GGNNCC 4 cut(s) 189, 564, 1197, 1491
BmsI GCATC 4 cut(s) 470, 1057, 1303, 1376
BpmI CTGGAG 2 cut(s) 549, 1272
Bpu10I CCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 1523
BsaJI CCNNGG 3 cut(s) 273, 1403, 1533
BsaXI ACNNNNNCTCC 4 cut(s) 547, 577, 1245, 1275
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 1338
Bse21I CCTNAGG 2 cut(s) 1126, 1191
Bse3DI GCAATG 2 cut(s) 683, 1458
Bse8I GATNNNNATC 1 cut(s) 1523
BseDI CCNNGG 3 cut(s) 273, 1403, 1533
BseGI GGATG 3 cut(s) 1263, 1498, 1521
BseJI GATNNNNATC 1 cut(s) 1523
BseLI CCNNNNNNNGG 2 cut(s) 101, 1338
BseMI GCAATG 2 cut(s) 683, 1458
BseMII CTCAG 4 cut(s) 669, 972, 1008, 1205
BseXI GCAGC 2 cut(s) 119, 1328
Bsh1236I CGCG 2 cut(s) 368, 1025
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 1489
BsiEI CGRYCG 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 826
BslFI GGGAC 3 cut(s) 75, 568, 1249
BslI CCNNNNNNNGG 2 cut(s) 101, 1338
BsmAI GTCTC 2 cut(s) 275, 1178
BsmBI CGTCTC 1 cut(s) 1178
BsmFI GGGAC 3 cut(s) 75, 568, 1249
BsmI GAATGC 1 cut(s) 144
BsnI GGCC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 826
Bsp119I TTCGAA 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 1533
BspACI CCGC 3 cut(s) 366, 606, 1025
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 4 cut(s) 670, 973, 1007, 1204
BspFNI CGCG 2 cut(s) 368, 1025
BspLI GGNNCC 4 cut(s) 189, 564, 1197, 1491
BspPI GGATC 7 cut(s) 50, 218, 632, 845, 1190, 1203, 1315
BspT104I TTCGAA 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 1489
BsrDI GCAATG 2 cut(s) 683, 1458
BssECI CCNNGG 3 cut(s) 273, 1403, 1533
BssT1I CCWWGG 3 cut(s) 273, 1403, 1533
Bst4CI ACNGT 4 cut(s) 120, 181, 412, 1558
Bst6I CTCTTC 2 cut(s) 446, 910
BstBI TTCGAA 1 cut(s) 375
BstDEI CTNAG 8 cut(s) 395, 591, 678, 881, 981, 994, 1126, 1191
BstDSI CCRYGG 1 cut(s) 1533
BstF5I GGATG 3 cut(s) 1263, 1498, 1521
BstFNI CGCG 2 cut(s) 368, 1025
BstHHI GCGC 1 cut(s) 1025
BstMAI GTCTC 2 cut(s) 275, 1178
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 148, 1394
BstNSI RCATGY 1 cut(s) 1151
BstSFI CTRYAG 1 cut(s) 411
BstUI CGCG 2 cut(s) 368, 1025
BstV1I GCAGC 2 cut(s) 119, 1328
BstX2I RGATCY 3 cut(s) 42, 637, 1195
BstXI CCANNNNNNTGG 1 cut(s) 90
BstYI RGATCY 3 cut(s) 42, 637, 1195
Bsu36I CCTNAGG 2 cut(s) 1126, 1191
BsuI GTATCC 1 cut(s) 1570
BsuRI GGCC 1 cut(s) 94
BtgI CCRYGG 1 cut(s) 1533
BtsCI GGATG 3 cut(s) 1263, 1498, 1521
BtsIMutI CAGTG 1 cut(s) 850
CfoI GCGC 1 cut(s) 1025
Cfr13I GGNCC 2 cut(s) 80, 1400
Csp6I GTAC 2 cut(s) 408, 1271
CviAII CATG 6 cut(s) 39, 143, 955, 1148, 1534, 1541
CviQI GTAC 2 cut(s) 408, 1271
DdeI CTNAG 8 cut(s) 395, 591, 678, 881, 981, 994, 1126, 1191
DraI TTTAAA 2 cut(s) 421, 1327
Eam1104I CTCTTC 2 cut(s) 446, 910
EarI CTCTTC 2 cut(s) 446, 910
Eco130I CCWWGG 3 cut(s) 273, 1403, 1533
Eco47I GGWCC 2 cut(s) 80, 1400
Eco81I CCTNAGG 2 cut(s) 1126, 1191
Eco88I CYCGRG 1 cut(s) 826
EcoRI GAATTC 1 cut(s) 1097
EcoT14I CCWWGG 3 cut(s) 273, 1403, 1533
EcoT22I ATGCAT 2 cut(s) 144, 1369
ErhI CCWWGG 3 cut(s) 273, 1403, 1533
Esp3I CGTCTC 1 cut(s) 1178
FaeI CATG 6 cut(s) 42, 146, 958, 1151, 1537, 1544
FaqI GGGAC 3 cut(s) 75, 568, 1249
FatI CATG 6 cut(s) 38, 142, 954, 1147, 1533, 1540
FauNDI CATATG 1 cut(s) 1267
FbaI TGATCA 1 cut(s) 1155
Fnu4HI GCNGC 2 cut(s) 108, 1317
FokI GGATG 3 cut(s) 1270, 1485, 1528
Fsp4HI GCNGC 2 cut(s) 108, 1317
GlaI GCGC 1 cut(s) 1024
GluI GCNGC 2 cut(s) 108, 1317
GsuI CTGGAG 2 cut(s) 549, 1272
HaeIII GGCC 1 cut(s) 94
HhaI GCGC 1 cut(s) 1025
Hin1II CATG 6 cut(s) 42, 146, 958, 1151, 1537, 1544
Hin6I GCGC 1 cut(s) 1023
HinP1I GCGC 1 cut(s) 1023
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 7 cut(s) 391, 518, 580, 1121, 1353, 1424, 1470
HpaI GTTAAC 1 cut(s) 123
HphI GGTGA 3 cut(s) 712, 820, 1362
Hpy166II GTNNAC 2 cut(s) 123, 1383
Hpy188I TCNGA 6 cut(s) 334, 679, 997, 1200, 1312, 1515
Hpy188III TCNNGA 8 cut(s) 174, 247, 566, 826, 838, 854, 1193, 1563
Hpy8I GTNNAC 2 cut(s) 123, 1383
HpyAV CCTTC 4 cut(s) 735, 1081, 1087, 1543
HpyCH4III ACNGT 4 cut(s) 120, 181, 412, 1558
HpyCH4IV ACGT 2 cut(s) 234, 1567
HpyCH4V TGCA 7 cut(s) 142, 490, 1048, 1316, 1367, 1438, 1467
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 1394
HpyF3I CTNAG 8 cut(s) 395, 591, 678, 881, 981, 994, 1126, 1191
HpySE526I ACGT 2 cut(s) 234, 1567
Hsp92II CATG 6 cut(s) 42, 146, 958, 1151, 1537, 1544
HspAI GCGC 1 cut(s) 1023
Ksp22I TGATCA 1 cut(s) 1155
KspAI GTTAAC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 104, 568
Lsp1109I GCAGC 2 cut(s) 119, 1328
LweI GCATC 4 cut(s) 470, 1057, 1303, 1376
MaeII ACGT 2 cut(s) 234, 1567
MaeIII GTNAC 8 cut(s) 31, 570, 619, 845, 1090, 1224, 1261, 1552
MboII GAAGA 7 cut(s) 37, 433, 661, 750, 927, 1226, 1456
MfeI CAATTG 1 cut(s) 1300
MflI RGATCY 3 cut(s) 42, 637, 1195
MluCI AATT 8 cut(s) 127, 265, 328, 377, 494, 1072, 1097, 1300
MlyI GAGTC 1 cut(s) 1362
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 9 cut(s) 342, 651, 673, 754, 822, 905, 1057, 1200, 1247
Mph1103I ATGCAT 2 cut(s) 144, 1369
MroXI GAANNNNTTC 3 cut(s) 659, 1167, 1423
MseI TTAA 6 cut(s) 122, 420, 435, 666, 1326, 1344
MslI CAYNNNNRTG 1 cut(s) 959
MssI GTTTAAAC 1 cut(s) 1327
MunI CAATTG 1 cut(s) 1300
Mva1269I GAATGC 1 cut(s) 144
MvnI CGCG 2 cut(s) 368, 1025
MwoI GCNNNNNNNGC 2 cut(s) 148, 1394
NcoI CCATGG 1 cut(s) 1533
NdeI CATATG 1 cut(s) 1267
NlaIII CATG 6 cut(s) 42, 146, 958, 1151, 1537, 1544
NlaIV GGNNCC 4 cut(s) 189, 564, 1197, 1491
NmuCI GTSAC 2 cut(s) 845, 1261
NsiI ATGCAT 2 cut(s) 144, 1369
NspI RCATGY 1 cut(s) 1151
NspV TTCGAA 1 cut(s) 375
PaeR7I CTCGAG 1 cut(s) 826
PctI GAATGC 1 cut(s) 144
PdmI GAANNNNTTC 3 cut(s) 659, 1167, 1423
PfeI GAWTC 6 cut(s) 391, 518, 580, 1121, 1424, 1470
PkrI GCNGC 2 cut(s) 109, 1318
Ple19I CGATCG 1 cut(s) 114
PleI GAGTC 1 cut(s) 1361
PmeI GTTTAAAC 1 cut(s) 1327
PpsI GAGTC 1 cut(s) 1361
PsiI TTATAA 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 234
PspN4I GGNNCC 4 cut(s) 189, 564, 1197, 1491
PspPI GGNCC 2 cut(s) 80, 1400
PsuI RGATCY 3 cut(s) 42, 637, 1195
PvuI CGATCG 1 cut(s) 114
RsaI GTAC 2 cut(s) 409, 1272
RsaNI GTAC 2 cut(s) 408, 1271
RseI CAYNNNNRTG 1 cut(s) 959
SaqAI TTAA 6 cut(s) 122, 420, 435, 666, 1326, 1344
SatI GCNGC 2 cut(s) 108, 1317
Sau96I GGNCC 2 cut(s) 80, 1400
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 1362
SfaNI GCATC 4 cut(s) 470, 1057, 1303, 1376
SfcI CTRYAG 1 cut(s) 411
Sfr274I CTCGAG 1 cut(s) 826
SfuI TTCGAA 1 cut(s) 375
SinI GGWCC 2 cut(s) 80, 1400
SlaI CTCGAG 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 959
SmlI CTYRAG 2 cut(s) 172, 826
SmoI CTYRAG 2 cut(s) 172, 826
Sse9I AATT 8 cut(s) 127, 265, 328, 377, 494, 1072, 1097, 1300
SsiI CCGC 3 cut(s) 366, 606, 1025
StyI CCWWGG 3 cut(s) 273, 1403, 1533
TaaI ACNGT 4 cut(s) 120, 181, 412, 1558
TaiI ACGT 2 cut(s) 237, 1570
TaqI TCGA 6 cut(s) 114, 136, 375, 827, 837, 1011
TasI AATT 8 cut(s) 127, 265, 328, 377, 494, 1072, 1097, 1300
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 6 cut(s) 391, 518, 580, 1121, 1424, 1470
Tru1I TTAA 6 cut(s) 122, 420, 435, 666, 1326, 1344
Tru9I TTAA 6 cut(s) 122, 420, 435, 666, 1326, 1344
TscAI CASTG 1 cut(s) 850
TseFI GTSAC 2 cut(s) 845, 1261
TseI GCWGC 2 cut(s) 107, 1316
Tsp45I GTSAC 2 cut(s) 845, 1261
TspDTI ATGAA 7 cut(s) 622, 668, 980, 984, 1457, 1472, 1560
TspRI CASTG 1 cut(s) 850
VpaK11BI GGWCC 2 cut(s) 80, 1400
XapI RAATTY 2 cut(s) 377, 1097
XceI RCATGY 1 cut(s) 1151
XhoI CTCGAG 1 cut(s) 826
XmnI GAANNNNTTC 3 cut(s) 659, 1167, 1423
ZrmI AGTACT 1 cut(s) 409
Zsp2I ATGCAT 2 cut(s) 144, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.