Rh4DG376300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011009
Physical Location & Seq
Forward (+)
59429270 .. 59430048
779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG376300.1

Sequence Viewer

Length: 702 bp
ATGAGGATGACTCTGGACTCCATATGTAAGGTGGGATTTGGTGTAGAGATTGGAACCCTGGCTCCAGATCTACCAGATAATCAGTTTGCCCAGGCCTTCGACACTGCAAACATAATTGTCACATATCGATTCATTGATCCACTATGGAAAATAAAGAAATTCTTAAATTGGGGATCAGAAGCTTTACTTGACAAGAGCATGAAAACCATAGATGATTTTACATATTCTGTAATTCGGAAGAGGAAATCAGAGATAAAAGAAGCTCAACAGAGTTCAGATAAAAACCTGATGAAGCATGATATACTGTCAAGATTCATCGAGTTAGGTGAAGACCCAGAAAGCAACTTAACTGACAAAAGCCTCAGGGATGTTGTCCTCAACTTCGTAATAGCCGGTCGGGACACAACAGCAACCACTCTCTCATGGGCGATTTACATGATAATGACCCATCCCGAGGTAGCTGAGAAGCTATATACGGAGCTTAAGACTCTTGAAGAAGAACAAGCACAAGAAGAGAAAGTTTCATTACTCCAATATGACAAAGAGGACCTTGGATCATTCAATCAAAGAGTGAAACAATTTGCAAGACTCATGAACTATGATTCATTGGGGAGACTCTATTATTTGCATGCAGTAATCACAGAGACACTCCGTCTGTACCCTGCAGTCCCTCAGGTATGCATCTTCCTCATTGGTCTATAA

Protein Analysis

233

Amino Acids

26.85

Weight (kDa)

5.23

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 175 1.5e-25 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69500
fragaria_vesca FvH4_4g31080 FvH4_4g31080
malus_domestica MD13G1069300.v1.1 MD16G1069600.v1.1
prunus_persica Prupe.1G277400_v2.0.a1
pyrus_communis pycom13g06210 pycom13g06220
rosa_chinensis RchiOBHm_Chr4g0439461
rosa_laevigata RLG00000006269
rosa_multiflora Rmu_sc0007276.1_g000005
rosa_roxburghii Rroxscaffold_5G00380380
rosa_rugosa Rorug04G0318500
rosa_samantha Rh4AG369800 Rh4BG381600 Rh4BG381700 Rh4CG396300 Rh4DG376200 Rh4DG376300
rosa_wichuraiana Rw4G031530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 131, 181, 562
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 1 cut(s) 659
AfiI CCNNNNNNNGG 1 cut(s) 454
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 2 cut(s) 494, 562
AhdI GACNNNNNGTC 1 cut(s) 651
AjnI CCWGG 2 cut(s) 57, 90
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 5 cut(s) 182, 263, 461, 469, 481
AluI AGCT 5 cut(s) 182, 263, 461, 469, 481
Alw26I GTCTC 2 cut(s) 607, 638
AlwI GGATC 3 cut(s) 131, 181, 562
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 547
AxyI CCTNAGG 2 cut(s) 362, 672
BbsI GAAGAC 1 cut(s) 336
BccI CCATC 1 cut(s) 456
BciT130I CCWGG 2 cut(s) 59, 92
BcoDI GTCTC 2 cut(s) 607, 638
BfmI CTRYAG 1 cut(s) 663
BfrI CTTAAG 1 cut(s) 482
BglII AGATCT 1 cut(s) 67
Bme1390I CCNGG 2 cut(s) 59, 92
Bme18I GGWCC 1 cut(s) 547
BmeRI GACNNNNNGTC 1 cut(s) 651
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 2 cut(s) 55, 63
BmrFI CCNGG 2 cut(s) 59, 92
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 336
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 48
Bsa29I ATCGAT 1 cut(s) 127
BsaJI CCNNGG 4 cut(s) 57, 90, 453, 550
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse118I RCCGGY 1 cut(s) 392
Bse21I CCTNAGG 2 cut(s) 362, 672
BseBI CCWGG 2 cut(s) 59, 92
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 4 cut(s) 57, 90, 453, 550
BseGI GGATG 3 cut(s) 12, 373, 448
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMII CTCAG 3 cut(s) 376, 453, 686
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 1 cut(s) 95
BshVI ATCGAT 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 2 cut(s) 413, 653
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 2 cut(s) 607, 638
BsmFI GGGAC 2 cut(s) 413, 653
BsnI GGCC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 452
Bsp143I GATC 4 cut(s) 67, 136, 173, 554
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 3 cut(s) 375, 454, 685
BspDI ATCGAT 1 cut(s) 127
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 55, 63
BspMAI CTGCAG 1 cut(s) 667
BspPI GGATC 3 cut(s) 131, 181, 562
BspTI CTTAAG 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 4 cut(s) 57, 90, 453, 550
BssMI GATC 4 cut(s) 67, 136, 173, 554
BssT1I CCWWGG 1 cut(s) 550
Bst2UI CCWGG 2 cut(s) 59, 92
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 2 cut(s) 233, 507
BstAFI CTTAAG 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 3 cut(s) 362, 462, 672
BstF5I GGATG 3 cut(s) 12, 373, 448
BstKTI GATC 4 cut(s) 70, 139, 176, 557
BstMAI GTCTC 2 cut(s) 607, 638
BstMBI GATC 4 cut(s) 67, 136, 173, 554
BstMCI CGRYCG 1 cut(s) 397
BstNI CCWGG 2 cut(s) 59, 92
BstNSI RCATGY 1 cut(s) 632
BstSCI CCNGG 2 cut(s) 57, 90
BstSFI CTRYAG 1 cut(s) 663
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
Bsu15I ATCGAT 1 cut(s) 127
Bsu36I CCTNAGG 2 cut(s) 362, 672
BsuRI GGCC 1 cut(s) 95
BsuTUI ATCGAT 1 cut(s) 127
BtsCI GGATG 3 cut(s) 12, 373, 448
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 630
CciI TCATGA 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 547
ClaI ATCGAT 1 cut(s) 127
Csp6I GTAC 1 cut(s) 658
CviAII CATG 6 cut(s) 199, 296, 423, 436, 592, 629
CviJI RGCY 9 cut(s) 62, 95, 182, 263, 360, 392, 461, 469, 481
CviKI_1 RGCY 9 cut(s) 62, 95, 182, 263, 360, 392, 461, 469, 481
CviQI GTAC 1 cut(s) 658
DdeI CTNAG 3 cut(s) 362, 462, 672
DpnI GATC 4 cut(s) 69, 138, 175, 556
DpnII GATC 4 cut(s) 67, 136, 173, 554
DriI GACNNNNNGTC 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 233, 507
Eam1105I GACNNNNNGTC 1 cut(s) 651
EarI CTCTTC 2 cut(s) 233, 507
Eco130I CCWWGG 1 cut(s) 550
Eco147I AGGCCT 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 547
Eco81I CCTNAGG 2 cut(s) 362, 672
Eco88I CYCGRG 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 57, 90
EcoT14I CCWWGG 1 cut(s) 550
EcoT22I ATGCAT 1 cut(s) 683
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 6 cut(s) 202, 299, 426, 439, 595, 632
FalI AAGNNNNNCTT 6 cut(s) 146, 178, 171, 203, 534, 566
FaqI GGGAC 2 cut(s) 413, 653
FatI CATG 6 cut(s) 198, 295, 422, 435, 591, 628
FauNDI CATATG 1 cut(s) 23
FokI GGATG 3 cut(s) 19, 380, 435
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 393
Hin1II CATG 6 cut(s) 202, 299, 426, 439, 595, 632
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 8 cut(s) 10, 17, 129, 312, 487, 588, 602, 615
HpaII CCGG 1 cut(s) 393
HphI GGTGA 1 cut(s) 338
Hpy188I TCNGA 4 cut(s) 178, 237, 250, 277
Hpy188III TCNNGA 7 cut(s) 14, 65, 309, 398, 452, 491, 592
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4V TGCA 6 cut(s) 107, 584, 628, 632, 665, 681
HpyF3I CTNAG 3 cut(s) 362, 462, 672
Hsp92II CATG 6 cut(s) 202, 299, 426, 439, 595, 632
Kzo9I GATC 4 cut(s) 67, 136, 173, 554
LmnI GCTCC 2 cut(s) 67, 478
LweI GCATC 1 cut(s) 690
MaeIII GTNAC 1 cut(s) 118
MalI GATC 4 cut(s) 69, 138, 175, 556
MboI GATC 4 cut(s) 67, 136, 173, 554
MboII GAAGA 6 cut(s) 250, 341, 506, 509, 524, 676
MflI RGATCY 1 cut(s) 67
MluCI AATT 5 cut(s) 114, 158, 166, 231, 578
MlyI GAGTC 5 cut(s) 4, 11, 481, 582, 609
MnlI CCTC 7 cut(s) 234, 371, 386, 448, 538, 681, 698
Mph1103I ATGCAT 1 cut(s) 683
MseI TTAA 3 cut(s) 164, 347, 483
MslI CAYNNNNRTG 1 cut(s) 440
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 2 cut(s) 59, 92
MvaI CCWGG 2 cut(s) 59, 92
NdeI CATATG 1 cut(s) 23
NdeII GATC 4 cut(s) 67, 136, 173, 554
NlaIII CATG 6 cut(s) 202, 299, 426, 439, 595, 632
NlaIV GGNNCC 2 cut(s) 55, 63
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 1 cut(s) 683
NspI RCATGY 1 cut(s) 632
PaeI GCATGC 1 cut(s) 632
PagI TCATGA 1 cut(s) 591
PceI AGGCCT 1 cut(s) 95
PfeI GAWTC 3 cut(s) 129, 312, 602
PleI GAGTC 5 cut(s) 4, 11, 481, 582, 609
PpsI GAGTC 5 cut(s) 4, 11, 481, 582, 609
PpuMI RGGWCCY 1 cut(s) 547
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 57, 90
PspGI CCWGG 2 cut(s) 57, 90
PspN4I GGNNCC 2 cut(s) 55, 63
PspPI GGNCC 1 cut(s) 547
PspPPI RGGWCCY 1 cut(s) 547
PstI CTGCAG 1 cut(s) 667
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 1 cut(s) 659
RsaNI GTAC 1 cut(s) 658
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 3 cut(s) 164, 347, 483
Sau3AI GATC 4 cut(s) 67, 136, 173, 554
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 5 cut(s) 4, 11, 481, 582, 609
ScrFI CCNGG 2 cut(s) 59, 92
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SphI GCATGC 1 cut(s) 632
Sse9I AATT 5 cut(s) 114, 158, 166, 231, 578
SseBI AGGCCT 1 cut(s) 95
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 57, 90
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 99, 127, 318
TasI AATT 5 cut(s) 114, 158, 166, 231, 578
TfiI GAWTC 3 cut(s) 129, 312, 602
Tru1I TTAA 3 cut(s) 164, 347, 483
Tru9I TTAA 3 cut(s) 164, 347, 483
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 7 cut(s) 121, 215, 304, 305, 513, 594, 608
TspGWI ACGGA 2 cut(s) 491, 641
TspRI CASTG 1 cut(s) 109
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.