Rh4CG396300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011009
Physical Location & Seq
Forward (+)
64604990 .. 64607029
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG396300.1

Sequence Viewer

Length: 1191 bp
ATGGGATATCTTTTATGGGATTCTTCTTCAGAAGATGGATGCAGTAAGGGATGGTTGATGCTAGCTTGCACGGTTTTGTTATGGATCATTATCTATAGATGGAGCCAGAGGAACAGAAAAGGTCCCAAAACATGGCCGTTAGTAGGAGCAGCAATTGAACAACTCATGAACTATAACAGAATGCATGATTGGCTTGTCAAGTACCTGTCCGAATCAAACTCTGTTGTCGTCCCAATGCCGTTCACAACTTACACTTACATTGCAGATCCAGCTAATGTAGAACATGTCCTCAAGACCAACTTTGCGAATTACCCAAAGGAATTGCTGATGAGGATGACTCTGGACTCCATATGTAAGGTGGGATTTGGTGTAGAGATTGGAACCCTGGCTCCAGATCTACCAGATAATCAGTTTGCCCAGGCCTTCGACACTGCAAACATAATTGTCACATATCGATTCATTGATCCACTATGGAAAATAAAGAAATTCTTAAATTGGGGATCAGAAGCTTTACTTGACAAGAGCATGAAAACCATAGATGATTTTACATATTCTGTAATTCGGAAAAGGAAATCAGAGATAAAAGAAGCTCAACAGAGTTCAGATAAAAACCTGATGAAGCATGATATACTGTCAAGATTCATCGAGTTAGGTGAAGACCCAGAAAGCAACTTAACTGACAAAAGCCTCAGGGATGTTGTCCTCAACTTCGTAATAGCTGGTCGAGACACAACAGCAACCACTCTCTCATGGGCGATTTACATGATAATGACCCATCCCGAGGTAGCCGAGAAGCTATATACGGAGCTTAAGACTCTTGAAGAAGAACAAGCACGAGAAGAGAAAGATTCATTACTCCAATATGACAAAGAGGACCTTGAATCATTCAATCAAAGAGTGAAACAATTTGCAGGACTCATGAACTATGATTCATTGGGGAGACTCTATTATTTGCATGCAGTCATCACAGAGACACTCCGTCTGTACCCTGCAGTCCCTCAGGCAGGACCAAGAATATGCCTAGGGAAGGACTCTGCATACCTCCAAATGAAGATGGTACTGGCCATTCTGTGCAGATTTTTCACATTCACTTTGGTTCCCGGTCATCCAGTTGAGTATAGGATGATGACAATTTTATCAATGAAGCATGGCTTGAAGCTTACAGTAGCCAGACGCTCATCAGATGCATGA

Protein Analysis

396

Amino Acids

45.63

Weight (kDa)

6.68

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 105 - 333 2.2e-31 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69500
fragaria_vesca FvH4_4g31080 FvH4_4g31080
malus_domestica MD13G1069300.v1.1 MD16G1069600.v1.1
prunus_persica Prupe.1G277400_v2.0.a1
pyrus_communis pycom13g06210 pycom13g06220
rosa_chinensis RchiOBHm_Chr4g0439461
rosa_laevigata RLG00000006269
rosa_multiflora Rmu_sc0007276.1_g000005
rosa_roxburghii Rroxscaffold_5G00380380
rosa_rugosa Rorug04G0318500
rosa_samantha Rh4AG369800 Rh4BG381600 Rh4BG381700 Rh4CG396300 Rh4DG376200 Rh4DG376300
rosa_wichuraiana Rw4G031530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 132
AclWI GGATC 4 cut(s) 92, 260, 458, 508
AcoI YGGCCR 2 cut(s) 134, 1062
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 3 cut(s) 203, 986, 1059
AfiI CCNNNNNNNGG 5 cut(s) 132, 143, 781, 1004, 1027
AflII CTTAAG 1 cut(s) 809
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 5 cut(s) 158, 821, 881, 889, 1156
AhdI GACNNNNNGTC 1 cut(s) 978
AjnI CCWGG 2 cut(s) 384, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 172, 204
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 8 cut(s) 65, 272, 509, 590, 719, 796, 808, 1159
AluI AGCT 8 cut(s) 65, 272, 509, 590, 719, 796, 808, 1159
Alw26I GTCTC 3 cut(s) 720, 934, 965
AlwI GGATC 4 cut(s) 92, 260, 458, 508
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 3 cut(s) 134, 420, 1062
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 485
AspA2I CCTAGG 1 cut(s) 1021
AspS9I GGNCC 3 cut(s) 122, 874, 1007
AsuC2I CCSGG 1 cut(s) 1101
AsuHPI GGTGA 1 cut(s) 665
AsuNHI GCTAGC 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 3 cut(s) 122, 874, 1007
AvrII CCTAGG 1 cut(s) 1021
AxyI CCTNAGG 2 cut(s) 689, 999
BalI TGGCCA 1 cut(s) 1064
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 663
BbvI GCAGC 1 cut(s) 161
BccI CCATC 5 cut(s) 29, 45, 93, 783, 1048
BceAI ACGGC 2 cut(s) 121, 223
BciT130I CCWGG 2 cut(s) 386, 419
BcnI CCSGG 1 cut(s) 1101
BcoDI GTCTC 3 cut(s) 720, 934, 965
BfaI CTAG 2 cut(s) 62, 1022
BfmI CTRYAG 2 cut(s) 94, 990
BfrI CTTAAG 1 cut(s) 809
BglII AGATCT 1 cut(s) 394
BisI GCNGC 1 cut(s) 150
BlnI CCTAGG 1 cut(s) 1021
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 3 cut(s) 386, 419, 1101
Bme18I GGWCC 3 cut(s) 122, 874, 1007
BmeRI GACNNNNNGTC 1 cut(s) 978
BmeT110I CYCGRG 1 cut(s) 779
BmgT120I GGNCC 3 cut(s) 122, 874, 1007
BmiI GGNNCC 5 cut(s) 104, 124, 382, 390, 1098
BmrFI CCNGG 3 cut(s) 386, 419, 1101
BmsI GCATC 3 cut(s) 29, 48, 1174
BmtI GCTAGC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 663
BplI GAGNNNNNCTC 2 cut(s) 322, 354
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 1101
Bsa29I ATCGAT 1 cut(s) 454
BsaBI GATNNNNATC 1 cut(s) 89
BsaJI CCNNGG 4 cut(s) 384, 417, 780, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 143, 781, 1004, 1027
Bse1I ACTGG 2 cut(s) 1065, 1109
Bse21I CCTNAGG 2 cut(s) 689, 999
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 2 cut(s) 386, 419
BseCI ATCGAT 1 cut(s) 454
BseDI CCNNGG 4 cut(s) 384, 417, 780, 1021
BseGI GGATG 7 cut(s) 44, 56, 339, 700, 775, 1105, 1128
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 5 cut(s) 132, 143, 781, 1004, 1027
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 703, 1013
BseNI ACTGG 2 cut(s) 1065, 1109
BseXI GCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 1093
BshFI GGCC 3 cut(s) 136, 422, 1064
BshVI ATCGAT 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 779
BsiSI CCGG 1 cut(s) 1101
BslFI GGGAC 3 cut(s) 108, 215, 980
BslI CCNNNNNNNGG 5 cut(s) 132, 143, 781, 1004, 1027
BsmAI GTCTC 3 cut(s) 720, 934, 965
BsmFI GGGAC 3 cut(s) 108, 215, 980
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 3 cut(s) 136, 422, 1064
BsoBI CYCGRG 1 cut(s) 779
Bsp143I GATC 5 cut(s) 84, 265, 394, 463, 500
BspANI GGCC 3 cut(s) 136, 422, 1064
BspCNI CTCAG 2 cut(s) 702, 1012
BspDI ATCGAT 1 cut(s) 454
BspHI TCATGA 2 cut(s) 165, 918
BspLI GGNNCC 5 cut(s) 104, 124, 382, 390, 1098
BspMAI CTGCAG 1 cut(s) 994
BspOI GCTAGC 1 cut(s) 65
BspPI GGATC 4 cut(s) 92, 260, 458, 508
BspTI CTTAAG 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 2 cut(s) 1065, 1109
BssECI CCNNGG 4 cut(s) 384, 417, 780, 1021
BssMI GATC 5 cut(s) 84, 265, 394, 463, 500
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 1021
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 2 cut(s) 386, 419
Bst4CI ACNGT 3 cut(s) 73, 633, 1165
Bst6I CTCTTC 1 cut(s) 834
BstAFI CTTAAG 1 cut(s) 809
BstC8I GCNNGC 3 cut(s) 63, 67, 957
BstDEI CTNAG 2 cut(s) 689, 999
BstENI CCTNNNNNAGG 2 cut(s) 1002, 1025
BstF5I GGATG 7 cut(s) 44, 56, 339, 700, 775, 1105, 1128
BstKTI GATC 5 cut(s) 87, 268, 397, 466, 503
BstMAI GTCTC 3 cut(s) 720, 934, 965
BstMBI GATC 5 cut(s) 84, 265, 394, 463, 500
BstMWI GCNNNNNNNGC 2 cut(s) 190, 269
BstNI CCWGG 2 cut(s) 386, 419
BstNSI RCATGY 2 cut(s) 287, 959
BstSCI CCNGG 3 cut(s) 384, 417, 1099
BstSFI CTRYAG 2 cut(s) 94, 990
BstV1I GCAGC 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 2 cut(s) 265, 394
BstYI RGATCY 2 cut(s) 265, 394
Bsu15I ATCGAT 1 cut(s) 454
Bsu36I CCTNAGG 2 cut(s) 689, 999
BsuRI GGCC 3 cut(s) 136, 422, 1064
BsuTUI ATCGAT 1 cut(s) 454
BtsCI GGATG 7 cut(s) 44, 56, 339, 700, 775, 1105, 1128
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 3 cut(s) 63, 67, 957
CciI TCATGA 2 cut(s) 165, 918
Cfr13I GGNCC 3 cut(s) 122, 874, 1007
ClaI ATCGAT 1 cut(s) 454
CseI GACGC 1 cut(s) 1182
Csp6I GTAC 3 cut(s) 202, 985, 1058
CviQI GTAC 3 cut(s) 202, 985, 1058
DdeI CTNAG 2 cut(s) 689, 999
DpnI GATC 5 cut(s) 86, 267, 396, 465, 502
DpnII GATC 5 cut(s) 84, 265, 394, 463, 500
DriI GACNNNNNGTC 1 cut(s) 978
EaeI YGGCCR 2 cut(s) 134, 1062
Eam1104I CTCTTC 1 cut(s) 834
Eam1105I GACNNNNNGTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 834
Eco130I CCWWGG 1 cut(s) 1021
Eco147I AGGCCT 1 cut(s) 422
Eco32I GATATC 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 122, 874, 1007
Eco57I CTGAAG 1 cut(s) 12
Eco81I CCTNAGG 2 cut(s) 689, 999
Eco88I CYCGRG 1 cut(s) 779
EcoNI CCTNNNNNAGG 2 cut(s) 1002, 1025
EcoO109I RGGNCCY 2 cut(s) 122, 874
EcoRII CCWGG 2 cut(s) 384, 417
EcoRV GATATC 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 1021
EcoT22I ATGCAT 2 cut(s) 186, 1189
ErhI CCWWGG 1 cut(s) 1021
FaqI GGGAC 3 cut(s) 108, 215, 980
FauNDI CATATG 1 cut(s) 350
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 7 cut(s) 51, 63, 346, 707, 762, 1092, 1135
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 2 cut(s) 62, 1022
GluI GCNGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 3 cut(s) 136, 422, 1064
HapII CCGG 1 cut(s) 1101
HgaI GACGC 1 cut(s) 1182
HindIII AAGCTT 2 cut(s) 507, 1157
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 1 cut(s) 665
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 7 cut(s) 31, 211, 505, 564, 577, 604, 1183
Hpy188III TCNNGA 9 cut(s) 166, 292, 341, 392, 636, 725, 779, 818, 919
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 2 cut(s) 433, 1021
HpyCH4III ACNGT 3 cut(s) 73, 633, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 269
HpyF3I CTNAG 2 cut(s) 689, 999
Kzo9I GATC 5 cut(s) 84, 265, 394, 463, 500
LmnI GCTCC 4 cut(s) 102, 146, 394, 805
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 3 cut(s) 29, 48, 1174
MaeI CTAG 2 cut(s) 62, 1022
MaeIII GTNAC 1 cut(s) 445
MalI GATC 5 cut(s) 86, 267, 396, 465, 502
MboI GATC 5 cut(s) 84, 265, 394, 463, 500
MboII GAAGA 8 cut(s) 15, 18, 44, 668, 833, 836, 851, 1063
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 265, 394
MlsI TGGCCA 1 cut(s) 1064
MluCI AATT 9 cut(s) 153, 307, 320, 441, 485, 493, 558, 905, 1131
MluNI TGGCCA 1 cut(s) 1064
MlyI GAGTC 6 cut(s) 331, 338, 808, 909, 936, 1025
MnlI CCTC 9 cut(s) 102, 299, 324, 698, 713, 775, 865, 1008, 1052
Mox20I TGGCCA 1 cut(s) 1064
Mph1103I ATGCAT 2 cut(s) 186, 1189
MscI TGGCCA 1 cut(s) 1064
MseI TTAA 3 cut(s) 491, 674, 810
MslI CAYNNNNRTG 1 cut(s) 767
Msp20I TGGCCA 1 cut(s) 1064
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 3 cut(s) 386, 419, 1101
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 2 cut(s) 386, 419
MwoI GCNNNNNNNGC 2 cut(s) 190, 269
NciI CCSGG 1 cut(s) 1101
NdeI CATATG 1 cut(s) 350
NdeII GATC 5 cut(s) 84, 265, 394, 463, 500
NheI GCTAGC 1 cut(s) 61
NlaIV GGNNCC 5 cut(s) 104, 124, 382, 390, 1098
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 2 cut(s) 186, 1189
NspI RCATGY 2 cut(s) 287, 959
PaeI GCATGC 1 cut(s) 959
PagI TCATGA 2 cut(s) 165, 918
PceI AGGCCT 1 cut(s) 422
PciI ACATGT 1 cut(s) 283
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 7 cut(s) 20, 212, 456, 639, 848, 881, 929
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 6 cut(s) 331, 338, 808, 909, 936, 1025
PpsI GAGTC 6 cut(s) 331, 338, 808, 909, 936, 1025
PpuMI RGGWCCY 2 cut(s) 122, 874
PscI ACATGT 1 cut(s) 283
Psp5II RGGWCCY 2 cut(s) 122, 874
Psp6I CCWGG 2 cut(s) 384, 417
PspGI CCWGG 2 cut(s) 384, 417
PspN4I GGNNCC 5 cut(s) 104, 124, 382, 390, 1098
PspPI GGNCC 3 cut(s) 122, 874, 1007
PspPPI RGGWCCY 2 cut(s) 122, 874
PstI CTGCAG 1 cut(s) 994
PsuI RGATCY 2 cut(s) 265, 394
RsaI GTAC 3 cut(s) 203, 986, 1059
RsaNI GTAC 3 cut(s) 202, 985, 1058
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 3 cut(s) 491, 674, 810
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 5 cut(s) 84, 265, 394, 463, 500
Sau96I GGNCC 3 cut(s) 122, 874, 1007
SchI GAGTC 6 cut(s) 331, 338, 808, 909, 936, 1025
ScrFI CCNGG 3 cut(s) 386, 419, 1101
SfaNI GCATC 3 cut(s) 29, 48, 1174
SfcI CTRYAG 2 cut(s) 94, 990
SinI GGWCC 3 cut(s) 122, 874, 1007
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 290, 809
SmoI CTYRAG 2 cut(s) 290, 809
SphI GCATGC 1 cut(s) 959
Sse9I AATT 9 cut(s) 153, 307, 320, 441, 485, 493, 558, 905, 1131
SseBI AGGCCT 1 cut(s) 422
SspMI CTAG 2 cut(s) 62, 1022
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 3 cut(s) 384, 417, 1099
StyI CCWWGG 1 cut(s) 1021
TaaI ACNGT 3 cut(s) 73, 633, 1165
TaqI TCGA 4 cut(s) 426, 454, 645, 724
TasI AATT 9 cut(s) 153, 307, 320, 441, 485, 493, 558, 905, 1131
TfiI GAWTC 7 cut(s) 20, 212, 456, 639, 848, 881, 929
Tru1I TTAA 3 cut(s) 491, 674, 810
Tru9I TTAA 3 cut(s) 491, 674, 810
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 445
TspGWI ACGGA 2 cut(s) 818, 968
TspRI CASTG 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 132
Vha464I CTTAAG 1 cut(s) 809
VpaK11BI GGWCC 3 cut(s) 122, 874, 1007
XagI CCTNNNNNAGG 2 cut(s) 1002, 1025
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 287, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XmaJI CCTAGG 1 cut(s) 1021
XspI CTAG 2 cut(s) 62, 1022
Zsp2I ATGCAT 2 cut(s) 186, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.