pycom13g06220

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011009
Physical Location & Seq
Forward (+)
4047979 .. 4048749
771 bp
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UTR
Exon/CDS
Intron
pycom13g06220.1

Sequence Viewer

Length: 570 bp
ATGGTAATGACTCACCCCCATGTAGCTGAGAAGCTCTATTCCGAGCTGAAAACTTTTGAAGAAGATCAGGCAAGAGAAGAGAAGGTTTCGTTGCACCCGTATGACACAGAGAACCTTGAATTATTCAATCGAAGAGTTGCGCAATTTGCAGGACTGCTGAGTTACGACTCGCTGGGGAAACTGTATTATTTGCATGCTGTTATTACAGAGACGCTTCGTCTGTACCCTGCAGTCCCTCAGGATCCTAAAGGCATCTTGGAGGATGATGTTCTACCAGATGGAACCAAAGTCAAATACAATTGGGGACCTGATGCAGCTTCGTTTAAGCCCGAGAGATGGCTCAAAGAGGGTTTCTTCCAAAATGCATCGCCGTTCAAGTTCACCGCATTTCAGGCAGGACCAAGAATTTGCCTAGGGAAGGACTCTGCATACCTCCAAATGAAGATGGCCCTGGCCATTCTGTGCCGATTTTACACCTTCACTTTGGTCGCCGGGCATCCAGTTCAGTACCGGATGATGACAATTCTGTCAATGGCGCACGGCTTGAAGCTTACCATAGCCAGGCGCTAA

Protein Analysis

190

Amino Acids

21.68

Weight (kDa)

8.44

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 174 4.1e-20 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69500
fragaria_vesca FvH4_4g31080 FvH4_4g31080
malus_domestica MD13G1069300.v1.1 MD16G1069600.v1.1
prunus_persica Prupe.1G277400_v2.0.a1
pyrus_communis pycom13g06210 pycom13g06220
rosa_chinensis RchiOBHm_Chr4g0439461
rosa_laevigata RLG00000006269
rosa_multiflora Rmu_sc0007276.1_g000005
rosa_roxburghii Rroxscaffold_5G00380380
rosa_rugosa Rorug04G0318500
rosa_samantha Rh4AG369800 Rh4BG381600 Rh4BG381700 Rh4CG396300 Rh4DG376200 Rh4DG376300
rosa_wichuraiana Rw4G031530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 526
Acc16I TGCGCA 1 cut(s) 141
AciI CCGC 1 cut(s) 384
AclWI GGATC 2 cut(s) 236, 249
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 1 cut(s) 405
AfaI GTAC 2 cut(s) 224, 509
AfiI CCNNNNNNNGG 3 cut(s) 336, 418, 561
AgsI TTSAA 5 cut(s) 59, 119, 127, 376, 547
AhdI GACNNNNNGTC 1 cut(s) 216
AjnI CCWGG 2 cut(s) 450, 560
AluBI AGCT 5 cut(s) 26, 34, 46, 317, 550
AluI AGCT 5 cut(s) 26, 34, 46, 317, 550
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 2 cut(s) 236, 249
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 447, 453
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 1 cut(s) 405
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
AspA2I CCTAGG 1 cut(s) 412
AspLEI GCGC 3 cut(s) 142, 538, 567
AspS9I GGNCC 3 cut(s) 305, 398, 448
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 5, 373
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 2 cut(s) 305, 398
AvrII CCTAGG 1 cut(s) 412
AxyI CCTNAGG 1 cut(s) 237
BalI TGGCCA 1 cut(s) 455
BamHI GGATCC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 326
BccI CCATC 3 cut(s) 272, 330, 439
BceAI ACGGC 2 cut(s) 355, 556
BciT130I CCWGG 2 cut(s) 452, 562
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 568
BisI GCNGC 1 cut(s) 315
BlnI CCTAGG 1 cut(s) 412
BlsI GCNGC 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 452, 493, 562
Bme18I GGWCC 2 cut(s) 305, 398
BmeRI GACNNNNNGTC 1 cut(s) 216
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 3 cut(s) 305, 398, 448
BmiI GGNNCC 3 cut(s) 243, 283, 306
BmrFI CCNGG 3 cut(s) 452, 493, 562
BmsI GCATC 4 cut(s) 261, 301, 374, 505
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 412, 450
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 418, 561
Bse1I ACTGG 1 cut(s) 500
Bse21I CCTNAGG 1 cut(s) 237
BseBI CCWGG 2 cut(s) 452, 562
BseDI CCNNGG 2 cut(s) 412, 450
BseGI GGATG 3 cut(s) 268, 496, 519
BseLI CCNNNNNNNGG 3 cut(s) 336, 418, 561
BseMII CTCAG 3 cut(s) 18, 149, 251
BseNI ACTGG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 326
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 2 cut(s) 449, 455
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 2 cut(s) 492, 511
BslFI GGGAC 2 cut(s) 218, 318
BslI CCNNNNNNNGG 3 cut(s) 336, 418, 561
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 218, 318
BsnI GGCC 2 cut(s) 449, 455
BsoBI CYCGRG 1 cut(s) 329
Bsp143I GATC 2 cut(s) 64, 241
BspACI CCGC 1 cut(s) 384
BspANI GGCC 2 cut(s) 449, 455
BspCNI CTCAG 3 cut(s) 19, 150, 250
BspLI GGNNCC 3 cut(s) 243, 283, 306
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 2 cut(s) 236, 249
BsrI ACTGG 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 412, 450
BssMI GATC 2 cut(s) 64, 241
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 2 cut(s) 452, 562
Bst4CI ACNGT 1 cut(s) 183
Bst6I CTCTTC 2 cut(s) 72, 127
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 3 cut(s) 27, 158, 237
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 3 cut(s) 268, 496, 519
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 3 cut(s) 142, 538, 567
BstKTI GATC 2 cut(s) 67, 244
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 2 cut(s) 64, 241
BstMWI GCNNNNNNNGC 2 cut(s) 146, 392
BstNI CCWGG 2 cut(s) 452, 562
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 450, 491, 560
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 449, 455
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 268, 496, 519
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 3 cut(s) 142, 538, 567
Cfr13I GGNCC 3 cut(s) 305, 398, 448
CseI GACGC 1 cut(s) 220
Csp6I GTAC 2 cut(s) 223, 508
CviAII CATG 2 cut(s) 20, 194
CviQI GTAC 2 cut(s) 223, 508
DdeI CTNAG 3 cut(s) 27, 158, 237
DpnI GATC 2 cut(s) 66, 243
DpnII GATC 2 cut(s) 64, 241
DrdI GACNNNNNNGTC 1 cut(s) 526
DriI GACNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 2 cut(s) 72, 127
Eam1105I GACNNNNNGTC 1 cut(s) 216
EarI CTCTTC 2 cut(s) 72, 127
Eco130I CCWWGG 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 305, 398
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 450, 560
EcoT14I CCWWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 2 cut(s) 23, 197
FaiI YATR 5 cut(s) 21, 102, 195, 430, 557
FaqI GGGAC 2 cut(s) 218, 318
FatI CATG 2 cut(s) 19, 193
Fnu4HI GCNGC 1 cut(s) 315
FokI GGATG 3 cut(s) 275, 483, 526
Fsp4HI GCNGC 1 cut(s) 315
FspBI CTAG 1 cut(s) 413
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 3 cut(s) 141, 537, 566
GluI GCNGC 1 cut(s) 315
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 2 cut(s) 449, 455
HapII CCGG 2 cut(s) 492, 511
HgaI GACGC 1 cut(s) 220
HhaI GCGC 3 cut(s) 142, 538, 567
Hin1II CATG 2 cut(s) 23, 197
Hin6I GCGC 3 cut(s) 140, 536, 565
HinP1I GCGC 3 cut(s) 140, 536, 565
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 3 cut(s) 10, 167, 422
HpaII CCGG 2 cut(s) 492, 511
HphI GGTGA 2 cut(s) 5, 373
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 1 cut(s) 381
HpyAV CCTTC 3 cut(s) 76, 412, 487
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4V TGCA 7 cut(s) 94, 149, 193, 230, 314, 365, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 392
HpyF3I CTNAG 3 cut(s) 27, 158, 237
Hsp92II CATG 2 cut(s) 23, 197
HspAI GCGC 3 cut(s) 140, 536, 565
Kzo9I GATC 2 cut(s) 64, 241
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 4 cut(s) 261, 301, 374, 505
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 161
MalI GATC 2 cut(s) 66, 243
MboI GATC 2 cut(s) 64, 241
MboII GAAGA 6 cut(s) 71, 74, 89, 144, 346, 454
MfeI CAATTG 1 cut(s) 298
MflI RGATCY 1 cut(s) 241
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 5 cut(s) 119, 143, 298, 405, 522
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 3 cut(s) 4, 161, 416
MnlI CCTC 4 cut(s) 246, 253, 340, 443
Mox20I TGGCCA 1 cut(s) 455
Mph1103I ATGCAT 1 cut(s) 367
MscI TGGCCA 1 cut(s) 455
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 18, 99
Msp20I TGGCCA 1 cut(s) 455
MspI CCGG 2 cut(s) 492, 511
MspR9I CCNGG 3 cut(s) 452, 493, 562
MunI CAATTG 1 cut(s) 298
MvaI CCWGG 2 cut(s) 452, 562
MwoI GCNNNNNNNGC 2 cut(s) 146, 392
NciI CCSGG 1 cut(s) 493
NdeII GATC 2 cut(s) 64, 241
NlaIII CATG 2 cut(s) 23, 197
NlaIV GGNNCC 3 cut(s) 243, 283, 306
NsbI TGCGCA 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 1 cut(s) 197
PaeI GCATGC 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PkrI GCNGC 1 cut(s) 316
PleI GAGTC 3 cut(s) 4, 161, 416
PpsI GAGTC 3 cut(s) 4, 161, 416
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 450, 560
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 2 cut(s) 450, 560
PspN4I GGNNCC 3 cut(s) 243, 283, 306
PspPI GGNCC 3 cut(s) 305, 398, 448
PspPPI RGGWCCY 1 cut(s) 305
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 2 cut(s) 224, 509
RsaNI GTAC 2 cut(s) 223, 508
RseI CAYNNNNRTG 2 cut(s) 18, 99
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 2 cut(s) 64, 241
Sau96I GGNCC 3 cut(s) 305, 398, 448
SchI GAGTC 3 cut(s) 4, 161, 416
ScrFI CCNGG 3 cut(s) 452, 493, 562
SfaNI GCATC 4 cut(s) 261, 301, 374, 505
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 2 cut(s) 305, 398
SmiMI CAYNNNNRTG 2 cut(s) 18, 99
SphI GCATGC 1 cut(s) 197
Sse9I AATT 5 cut(s) 119, 143, 298, 405, 522
SsiI CCGC 1 cut(s) 384
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 450, 491, 560
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 130
TasI AATT 5 cut(s) 119, 143, 298, 405, 522
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 305, 398
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 405
XceI RCATGY 1 cut(s) 197
XmaJI CCTAGG 1 cut(s) 412
XspI CTAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.