AT1G70580

aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005109
Physical Location & Seq
Forward (+)
26612359 .. 26616071
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70580.3

Sequence Viewer

Length: 1446 bp
ATGTCTCTCAAGGCGTTAGACTACGAGTCCTTGAATGAAAACGTGAAGAATTGTCAGTATGCAGTCAGAGGTGAACTTTATCTTCGTGCTTCTGAGCTTCAGAAAGAAGGCAAAAAGATTATTTTCACAAATGTTGGAAACCCTCATGCTTTAGGACAGAAACCTCTGACTTTTCCTCGTCAGGTGGTTTCTTTATGCCAAGCACCATTTCTGTTAGATGATCCAAATGTTGGTATGATATTCCCAGCAGATGCTATTGCAAGAGCTAAGCATTATCTTTCCTTGACTTCTGGTGGTCTTGGTGCTTACAGTGACTCAAGAGGTCTTCCGGGAGTTCGGAAAGAAGTCGCTGAGTTCATTGAACGGCGTGATGGATATCCAAGCGATCCAGAACTCATATTTCTAACTGATGGAGCGAGCAAAGGTGTGATGCAAATCTTGAATTGTGTCATACGCGGTCAGAAAGACGGAATTCTGGTTCCAGTTCCACAGTATCCACTCTACTCGGCTACTATATCTCTGTTAGGTGGTACTCTTGTTCCTTACTATCTTGAAGAGTCTGAAAACTGGGGACTTGATGTTAACAACCTTCGCCAATCTGTTGCTCAAGCTCGCTCTCAAGGAATAACAGTAAGGGCAATGGTGATTATTAACCCCGGAAACCCAACTGGCCAGTGTCTTAGCGAAGCTAACATAAGAGAGATACTACGGTTCTGTTGTGATGAGAGATTAGTTCTTCTCGGAGACGAAGTGTATCAGCAAAATATATACCAAGATGAACGTCCCTTTATCAGTTCCAAGAAGGTTTTGATGGATATGGGAGCACCGATCAGCAAGGAAGTTCAGCTCATATCTTTCCACACCGTTTCCAAAGGATACTGGGGCGAATGTGGGCAACGGGGAGGTTACTTTGAGATGACAAATATCCCTCCCAGGACCGTTGAGGAGATATACAAGGTGGCCTCTATAGCTCTCAGCCCCAACGTCTCTGCGCAGATATTTATGGGTTTAATGGTTAGCCCACCAAAGCCTGGAGACATTTCATATGACCAATTCGTTCGTGAGAGCAAGGGAATACTAGAATCACTGAGAAGAAGAGCAAGGATGATGACTGATGGATTCAACAGCTGCAAAAACGTCGTCTGTAATTTCACAGAAGGTGCTATGTATTCATTCCCTCAAATAAAGTTGCCGTCGAAAGCAATCCAAGCAGCAAAACAAGCCGGAAAAGTCCCTGACGTTTTCTACTGCCTTAAGCTCTTAGAAGCCACAGGAATCTCCACAGTTCCAGGCTCTGGATTTGGACAAAAAGAAGGGGTGTTTCATTTAAGGACAACAATTCTGCCAGCAGAAGAAGAAATGCCAGAGATTATGGACAGTTTCAAAAAGTTCAATGATGAGTTTATGTCTCAGTACGCTGATAACTTTGGTTACTCCAGAATGTGA

Protein Analysis

481

Amino Acids

53.44

Weight (kDa)

6.21

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 84 - 459 1.2e-37 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 993
AccB7I CCANNNNNTGG 3 cut(s) 230, 1031, 1295
AccII CGCG 1 cut(s) 456
AciI CCGC 1 cut(s) 456
AclWI GGATC 2 cut(s) 215, 380
AcoI YGGCCR 1 cut(s) 670
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 532, 1415
AfiI CCNNNNNNNGG 3 cut(s) 230, 1031, 1295
AflII CTTAAG 1 cut(s) 1253
AgsI TTSAA 7 cut(s) 34, 362, 442, 554, 1123, 1384, 1393
AhdI GACNNNNNGTC 1 cut(s) 25
AjnI CCWGG 3 cut(s) 932, 1030, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 8 cut(s) 97, 266, 611, 689, 847, 971, 1128, 1258
AluI AGCT 8 cut(s) 97, 266, 611, 689, 847, 971, 1128, 1258
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 5 cut(s) 9, 738, 991, 1029, 1413
AlwI GGATC 2 cut(s) 215, 380
AlwNI CAGNNNCTG 1 cut(s) 1295
AoxI GGCC 2 cut(s) 670, 960
ApeKI GCWGC 2 cut(s) 1128, 1211
ApoI RAATTY 1 cut(s) 471
ArsI GACNNNNNNTTYG 2 cut(s) 1125, 1157
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 1 cut(s) 936
AsuC2I CCSGG 2 cut(s) 330, 657
AsuHPI GGTGA 2 cut(s) 83, 655
AvaII GGWCC 1 cut(s) 936
BaeI ACNNNNGTAYC 4 cut(s) 522, 555, 695, 728
BalI TGGCCA 1 cut(s) 672
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 2 cut(s) 1115, 1223
BccI CCATC 4 cut(s) 365, 404, 805, 1109
BceAI ACGGC 2 cut(s) 380, 1177
BcgI CGANNNNNNTGC 2 cut(s) 1120, 1154
BciT130I CCWGG 3 cut(s) 934, 1032, 1290
BciVI GTATCC 2 cut(s) 504, 869
BcnI CCSGG 2 cut(s) 330, 657
BcoDI GTCTC 5 cut(s) 9, 738, 991, 1029, 1413
BfaI CTAG 1 cut(s) 1079
BfmI CTRYAG 1 cut(s) 966
BfrI CTTAAG 1 cut(s) 1253
BfuI GTATCC 2 cut(s) 504, 869
BisI GCNGC 2 cut(s) 1129, 1212
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 2 cut(s) 1130, 1213
Bme1390I CCNGG 5 cut(s) 330, 657, 934, 1032, 1290
Bme18I GGWCC 1 cut(s) 936
BmeRI GACNNNNNGTC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 936
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 5 cut(s) 330, 657, 934, 1032, 1290
BmrI ACTGGG 2 cut(s) 577, 889
BmsI GCATC 2 cut(s) 241, 420
BmuI ACTGGG 2 cut(s) 577, 889
BpiI GAAGAC 1 cut(s) 317
BpmI CTGGAG 2 cut(s) 1053, 1420
Bpu1102I GCTNAGC 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 301, 591, 603
BpuMI CCSGG 2 cut(s) 330, 657
BsaBI GATNNNNATC 2 cut(s) 375, 434
BsaJI CCNNGG 2 cut(s) 655, 932
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 1031, 1295
Bse1I ACTGG 5 cut(s) 482, 572, 673, 673, 884
Bse3DI GCAATG 1 cut(s) 645
Bse8I GATNNNNATC 2 cut(s) 375, 434
BseBI CCWGG 3 cut(s) 934, 1032, 1290
BseDI CCNNGG 2 cut(s) 655, 932
BseGI GGATG 1 cut(s) 1110
BseJI GATNNNNATC 2 cut(s) 375, 434
BseLI CCNNNNNNNGG 3 cut(s) 230, 1031, 1295
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 5 cut(s) 84, 342, 988, 1079, 1424
BseNI ACTGG 5 cut(s) 482, 572, 673, 673, 884
BseRI GAGGAG 1 cut(s) 959
BseXI GCAGC 2 cut(s) 1115, 1223
BseYI CCCAGC 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 672, 962
BsiHKAI GWGCWC 1 cut(s) 826
BsiSI CCGG 3 cut(s) 329, 657, 1224
BslFI GGGAC 3 cut(s) 585, 768, 1217
BslI CCNNNNNNNGG 3 cut(s) 230, 1031, 1295
BsmAI GTCTC 5 cut(s) 9, 738, 991, 1029, 1413
BsmBI CGTCTC 2 cut(s) 738, 991
BsmFI GGGAC 3 cut(s) 585, 768, 1217
BsnI GGCC 2 cut(s) 672, 962
Bsp1286I GDGCHC 1 cut(s) 826
Bsp143I GATC 3 cut(s) 220, 385, 828
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 672, 962
BspCNI CTCAG 5 cut(s) 85, 343, 987, 1080, 1423
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 480
BspPI GGATC 2 cut(s) 215, 380
BspQI GCTCTTC 1 cut(s) 1090
BspTI CTTAAG 1 cut(s) 1253
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 5 cut(s) 482, 572, 673, 673, 884
BssECI CCNNGG 2 cut(s) 655, 932
BssMI GATC 3 cut(s) 220, 385, 828
Bst2UI CCWGG 3 cut(s) 934, 1032, 1290
Bst4CI ACNGT 8 cut(s) 311, 492, 631, 711, 865, 940, 1285, 1379
Bst6I CTCTTC 2 cut(s) 549, 1090
BstAFI CTTAAG 1 cut(s) 1253
BstC8I GCNNGC 3 cut(s) 418, 613, 1347
BstDEI CTNAG 8 cut(s) 93, 267, 351, 680, 974, 1088, 1261, 1410
BstF5I GGATG 1 cut(s) 1110
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 3 cut(s) 223, 388, 831
BstMAI GTCTC 5 cut(s) 9, 738, 991, 1029, 1413
BstMBI GATC 3 cut(s) 220, 385, 828
BstMWI GCNNNNNNNGC 3 cut(s) 968, 1208, 1220
BstNI CCWGG 3 cut(s) 934, 1032, 1290
BstSCI CCNGG 5 cut(s) 328, 655, 932, 1030, 1288
BstSFI CTRYAG 1 cut(s) 966
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 1115, 1223
BstV2I GAAGAC 1 cut(s) 317
BsuI GTATCC 2 cut(s) 504, 869
BsuRI GGCC 2 cut(s) 672, 962
BtsCI GGATG 1 cut(s) 1110
BtsIMutI CAGTG 3 cut(s) 316, 680, 1085
Cac8I GCNNGC 3 cut(s) 418, 613, 1347
CaiI CAGNNNCTG 1 cut(s) 1295
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 1 cut(s) 936
Csp6I GTAC 2 cut(s) 531, 1414
CviAII CATG 1 cut(s) 146
CviQI GTAC 2 cut(s) 531, 1414
DdeI CTNAG 8 cut(s) 93, 267, 351, 680, 974, 1088, 1261, 1410
DpnI GATC 3 cut(s) 222, 387, 830
DpnII GATC 3 cut(s) 220, 385, 828
DriI GACNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 670
Eam1104I CTCTTC 2 cut(s) 549, 1090
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 2 cut(s) 549, 1090
Eco32I GATATC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 936
Eco57I CTGAAG 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 3 cut(s) 932, 1030, 1288
EcoRV GATATC 1 cut(s) 377
Esp3I CGTCTC 2 cut(s) 738, 991
FaeI CATG 1 cut(s) 149
FaqI GGGAC 3 cut(s) 585, 768, 1217
FatI CATG 1 cut(s) 145
FauNDI CATATG 1 cut(s) 1045
Fnu4HI GCNGC 2 cut(s) 1129, 1212
FokI GGATG 1 cut(s) 1117
Fsp4HI GCNGC 2 cut(s) 1129, 1212
FspBI CTAG 1 cut(s) 1079
FspI TGCGCA 1 cut(s) 993
GlaI GCGC 1 cut(s) 993
GluI GCNGC 2 cut(s) 1129, 1212
GsaI CCCAGC 1 cut(s) 248
GsuI CTGGAG 2 cut(s) 1053, 1420
HaeIII GGCC 2 cut(s) 672, 962
HapII CCGG 3 cut(s) 329, 657, 1224
HhaI GCGC 1 cut(s) 994
Hin1II CATG 1 cut(s) 149
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 6 cut(s) 26, 314, 557, 1082, 1119, 1275
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 3 cut(s) 329, 657, 1224
HphI GGTGA 2 cut(s) 83, 655
Hpy166II GTNNAC 2 cut(s) 74, 583
Hpy188I TCNGA 8 cut(s) 68, 94, 102, 168, 339, 462, 562, 743
Hpy188III TCNNGA 7 cut(s) 318, 389, 439, 551, 1061, 1296, 1437
Hpy8I GTNNAC 2 cut(s) 74, 583
Hpy99I CGWCG 2 cut(s) 1142, 1198
HpyAV CCTTC 5 cut(s) 101, 599, 796, 1151, 1307
HpyCH4III ACNGT 8 cut(s) 311, 492, 631, 711, 865, 940, 1285, 1379
HpyCH4IV ACGT 5 cut(s) 42, 781, 984, 1137, 1239
HpyCH4V TGCA 4 cut(s) 62, 260, 433, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 968, 1208, 1220
HpyF3I CTNAG 8 cut(s) 93, 267, 351, 680, 974, 1088, 1261, 1410
HpySE526I ACGT 5 cut(s) 42, 781, 984, 1137, 1239
Hsp92II CATG 1 cut(s) 149
HspAI GCGC 1 cut(s) 992
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 3 cut(s) 220, 385, 828
LguI GCTCTTC 1 cut(s) 1090
LmnI GCTCC 2 cut(s) 413, 821
Lsp1109I GCAGC 2 cut(s) 1115, 1223
LweI GCATC 2 cut(s) 241, 420
MaeI CTAG 1 cut(s) 1079
MaeII ACGT 5 cut(s) 42, 781, 984, 1137, 1239
MaeIII GTNAC 3 cut(s) 311, 905, 1430
MalI GATC 3 cut(s) 222, 387, 830
MboI GATC 3 cut(s) 220, 385, 828
MboII GAAGA 9 cut(s) 58, 74, 317, 566, 728, 1104, 1107, 1364, 1367
MhlI GDGCHC 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 672
MluCI AATT 6 cut(s) 49, 442, 471, 1052, 1147, 1338
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 3 cut(s) 35, 308, 566
MmeI TCCRAC 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 672
MscI TGGCCA 1 cut(s) 672
MseI TTAA 5 cut(s) 582, 651, 1010, 1254, 1328
Msp20I TGGCCA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 1128
MspCI CTTAAG 1 cut(s) 1253
MspI CCGG 3 cut(s) 329, 657, 1224
MspR9I CCNGG 5 cut(s) 330, 657, 934, 1032, 1290
MvaI CCWGG 3 cut(s) 934, 1032, 1290
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 968, 1208, 1220
NciI CCSGG 2 cut(s) 330, 657
NdeI CATATG 1 cut(s) 1045
NdeII GATC 3 cut(s) 220, 385, 828
NlaIII CATG 1 cut(s) 149
NlaIV GGNNCC 1 cut(s) 480
NmeAIII GCCGAG 1 cut(s) 485
NmuCI GTSAC 1 cut(s) 311
NsbI TGCGCA 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 1090
PfeI GAWTC 3 cut(s) 1082, 1119, 1275
PflMI CCANNNNNTGG 3 cut(s) 230, 1031, 1295
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 2 cut(s) 1130, 1213
PleI GAGTC 3 cut(s) 34, 308, 565
PpsI GAGTC 3 cut(s) 34, 308, 565
Psp6I CCWGG 3 cut(s) 932, 1030, 1288
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 3 cut(s) 932, 1030, 1288
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 1 cut(s) 936
PstNI CAGNNNCTG 1 cut(s) 1295
PvuII CAGCTG 1 cut(s) 1128
RsaI GTAC 2 cut(s) 532, 1415
RsaNI GTAC 2 cut(s) 531, 1414
SapI GCTCTTC 1 cut(s) 1090
SaqAI TTAA 5 cut(s) 582, 651, 1010, 1254, 1328
SatI GCNGC 2 cut(s) 1129, 1212
Sau3AI GATC 3 cut(s) 220, 385, 828
Sau96I GGNCC 1 cut(s) 936
SchI GAGTC 3 cut(s) 35, 308, 566
ScrFI CCNGG 5 cut(s) 330, 657, 934, 1032, 1290
SduI GDGCHC 1 cut(s) 826
SfaNI GCATC 2 cut(s) 241, 420
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 1 cut(s) 936
SmlI CTYRAG 5 cut(s) 8, 316, 606, 618, 1253
SmoI CTYRAG 5 cut(s) 8, 316, 606, 618, 1253
Sse9I AATT 6 cut(s) 49, 442, 471, 1052, 1147, 1338
SsiI CCGC 1 cut(s) 456
SspMI CTAG 1 cut(s) 1079
StyD4I CCNGG 5 cut(s) 328, 655, 932, 1030, 1288
TaaI ACNGT 8 cut(s) 311, 492, 631, 711, 865, 940, 1285, 1379
TaiI ACGT 5 cut(s) 45, 784, 987, 1140, 1242
TaqI TCGA 1 cut(s) 1196
TasI AATT 6 cut(s) 49, 442, 471, 1052, 1147, 1338
TfiI GAWTC 3 cut(s) 1082, 1119, 1275
Tru1I TTAA 5 cut(s) 582, 651, 1010, 1254, 1328
Tru9I TTAA 5 cut(s) 582, 651, 1010, 1254, 1328
TscAI CASTG 3 cut(s) 316, 680, 1092
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 1128, 1211
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 6 cut(s) 51, 346, 792, 1032, 1161, 1313
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 3 cut(s) 316, 680, 1092
Van91I CCANNNNNTGG 3 cut(s) 230, 1031, 1295
Vha464I CTTAAG 1 cut(s) 1253
VpaK11BI GGWCC 1 cut(s) 936
XapI RAATTY 1 cut(s) 471
XspI CTAG 1 cut(s) 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.