AT1G72160

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012299
Physical Location & Seq
Reverse (-)
27153555 .. 27155793
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72160.1

Sequence Viewer

Length: 1473 bp
ATGGCTGAAGAACCTACTACTACCACTCTCGTTACACCGGAAAAGCTACCTTCTCCGAGCCTCACGCCTTCTGAAGTATCTGAATCTACTCAAGATGCCCTACCGACAGAGACAGAAACTCTGGAGAAAGTGACTGAGACTAATCCACCGGAAACTGCAGATACCACCACCAAGCCAGAAGAAGAAACCGCGGCAGAGCATCATCCACCGACAGTGACGGAAACAGAAACTGCATCGACGGAGAAACAAGAGGTTAAAGACGAAGCATCGCAGAAAGAAGTAGCTGAAGAGAAAAAGAGTATGATTCCACAGAATCTTGGTTCATTCAAAGAAGAAAGCAGCAAACTTTCTGATCTATCTAATTCCGAGAAGAAATCACTCGATGAACTAAAACATCTAGTTCGAGAAGCTCTAGACAATCACCAATTCACCAACACACCAGAAGAAGTCAAGATTTGGGGGATTCCATTACTTGAAGACGATAGAAGCGACGTCGTTTTGTTAAAATTCCTAAGAGCTAGGGAGTTCAAGGTGAAAGATTCGTTTGCTATGCTCAAGAACACAATCAAGTGGAGAAAGGAGTTCAAGATCGATGAATTGGTCGAGGAAGATCTTGTGGATGATCTTGACAAGGTTGTGTTTATGCATGGACATGACCGAGAAGGTCACCCTGTTTGTTACAATGTCTATGGTGAGTTTCAGAACAAGGAGCTTTATAATAAGACGTTTTCTGATGAGGAAAAGAGGAAACATTTCTTGAGGACTAGGATTCAGTTCTTGGAGAGGAGTATAAGGAAGCTAGATTTTAGCTCTGGTGGGGTTTCTACTATTTTTCAGGTTAATGATATGAAGAATTCTCCGGGGTTAGGGAAGAAAGAGCTTAGATCAGCTACTAAGCAAGCTGTTGAGTTGCTTCAGGACAATTACCCTGAGTTTGTCTTCAAACAGGCTTTTATCAATGTTCCTTGGTGGTACCTTGTGTTTTATACTGTGATTGGTCCGTTCATGACACCAAGATCAAAGAGCAAGCTTGTGTTTGCTGGTCCTTCGCGTTCAGCTGAAACCCTATTCAAATACATATCACCCGAGCAAGTTCCAGTACAATATGGTGGATTGAGTGTTGATCCTTGCGACTGCAATCCAGACTTTTCGTTGGAAGATTCAGCCTCTGAGATCACTGTTAAGCCCGGAACAAAACAAACTGTTGAGATCATAATCTATGAGAAATGTGAACTTGTGTGGGAGATAAGGGTAACTGGATGGGAAGTGAGCTACAAGGCTGAATTTGTGCCGGAAGAGAAAGATGCTTACACGGTGGTTATACAAAAACCGAGGAAGATGAGACCATCCGATGAACCGGTGTTAACCCATAGCTTCAAAGTGAATGAGCTTGGCAAGGTTTTACTCACAGTAGACAACCCAACCTCTAAGAAGAAGAAGCTCGTTTACAGGTTCAATGTCAAACCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

490

Amino Acids

56.1

Weight (kDa)

5.21

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 127 - 187 4.1e-11 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 211 - 371 5.2e-31 CRAL/TRIO domain
GOLD_PATL1_C PF25099 395 - 479 2.3e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72160
fragaria_vesca FvH4_5g31710 FvH4_5g31710
malus_domestica MD08G1218000.v1.1 MD15G1405900.v1.1
prunus_persica Prupe.1G555300_v2.0.a1
pyrus_communis pycom08g18900 pycom15g36270
rosa_chinensis RchiOBHm_Chr7g0227591
rosa_laevigata RLG00000001634
rosa_multiflora Rmu_sc0001772.1_g000008
rosa_roxburghii Rroxscaffold_3G00232020
rosa_rugosa Rorug07G0242000 Rorug07G0242100
rosa_samantha Rh7AG395500 Rh7BG377300 Rh7CG414200 Rh7DG391300
rosa_wichuraiana Rw7G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 972
AccB1I GGYRCC 1 cut(s) 972
AccI GTMKAC 1 cut(s) 1413
AccII CGCG 2 cut(s) 191, 1051
AciI CCGC 2 cut(s) 189, 191
AclWI GGATC 1 cut(s) 1118
AcsI RAATTY 3 cut(s) 506, 853, 1283
AcuI CTGAAG 4 cut(s) 27, 93, 306, 899
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 974, 1101
AfiI CCNNNNNNNGG 1 cut(s) 866
AgeI ACCGGT 1 cut(s) 1357
AgsI TTSAA 8 cut(s) 328, 476, 529, 586, 943, 1072, 1378, 1456
Alw26I GTCTC 3 cut(s) 104, 131, 1336
AlwI GGATC 1 cut(s) 1118
AlwNI CAGNNNCTG 2 cut(s) 230, 1169
Ama87I CYCGRG 1 cut(s) 1085
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 3 cut(s) 506, 853, 1283
AsiGI ACCGGT 1 cut(s) 1357
Asp700I GAANNNNTTC 1 cut(s) 752
Asp718I GGTACC 1 cut(s) 972
AspS9I GGNCC 2 cut(s) 998, 1043
AsuC2I CCSGG 2 cut(s) 861, 1188
AsuHPI GGTGA 6 cut(s) 413, 421, 544, 659, 704, 1074
AvaI CYCGRG 1 cut(s) 1085
AvaII GGWCC 2 cut(s) 998, 1043
BanI GGYRCC 1 cut(s) 972
BarI GAAGNNNNNNTAC 2 cut(s) 1430, 1462
BbsI GAAGAC 2 cut(s) 483, 931
BbvI GCAGC 1 cut(s) 351
BccI CCATC 2 cut(s) 1254, 1354
BcnI CCSGG 2 cut(s) 861, 1188
BcoDI GTCTC 3 cut(s) 104, 131, 1336
BfaI CTAG 5 cut(s) 398, 413, 519, 765, 800
BfmI CTRYAG 1 cut(s) 156
BglII AGATCT 1 cut(s) 610
BisI GCNGC 2 cut(s) 192, 340
BlsI GCNGC 2 cut(s) 193, 341
Bme1390I CCNGG 2 cut(s) 861, 1188
Bme18I GGWCC 2 cut(s) 998, 1043
BmeT110I CYCGRG 1 cut(s) 1085
BmgT120I GGNCC 2 cut(s) 998, 1043
BmiI GGNNCC 1 cut(s) 974
BmrFI CCNGG 2 cut(s) 861, 1188
BmsI GCATC 5 cut(s) 85, 208, 242, 275, 1294
BpiI GAAGAC 2 cut(s) 483, 931
BpmI CTGGAG 1 cut(s) 143
BpuEI CTTGAG 3 cut(s) 75, 539, 778
BpuMI CCSGG 2 cut(s) 861, 1188
Bsa29I ATCGAT 1 cut(s) 591
BsaBI GATNNNNATC 1 cut(s) 1214
BsaHI GRCGYC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 1336
BsaJI CCNNGG 4 cut(s) 189, 860, 965, 1331
BsaWI WCCGGW 3 cut(s) 37, 148, 1357
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse118I RCCGGY 1 cut(s) 1357
Bse1I ACTGG 2 cut(s) 1097, 1261
Bse8I GATNNNNATC 1 cut(s) 1214
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 4 cut(s) 189, 860, 965, 1331
BseGI GGATG 4 cut(s) 202, 625, 1265, 1346
BseJI GATNNNNATC 1 cut(s) 1214
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMII CTCAG 3 cut(s) 126, 921, 1161
BseNI ACTGG 2 cut(s) 1097, 1261
BseRI GAGGAG 1 cut(s) 799
BseXI GCAGC 1 cut(s) 351
Bsh1236I CGCG 2 cut(s) 191, 1051
BshNI GGYRCC 1 cut(s) 972
BshTI ACCGGT 1 cut(s) 1357
BshVI ATCGAT 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 1085
BsiSI CCGG 6 cut(s) 38, 149, 860, 1188, 1292, 1358
BslI CCNNNNNNNGG 1 cut(s) 866
BsmAI GTCTC 3 cut(s) 104, 131, 1336
Bso31I GGTCTC 1 cut(s) 1336
BsoBI CYCGRG 1 cut(s) 1085
Bsp143I GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
BspACI CCGC 2 cut(s) 189, 191
BspCNI CTCAG 3 cut(s) 127, 922, 1162
BspDI ATCGAT 1 cut(s) 591
BspFNI CGCG 2 cut(s) 191, 1051
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 1 cut(s) 974
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 1 cut(s) 1118
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 1 cut(s) 1336
BsrFI RCCGGY 1 cut(s) 1357
BsrI ACTGG 2 cut(s) 1097, 1261
BssAI RCCGGY 1 cut(s) 1357
BssECI CCNNGG 4 cut(s) 189, 860, 965, 1331
BssMI GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 965
Bst4CI ACNGT 6 cut(s) 214, 991, 1180, 1204, 1315, 1411
Bst6I CTCTTC 2 cut(s) 282, 1290
BstACI GRCGYC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 900, 1028
BstDEI CTNAG 7 cut(s) 135, 512, 881, 894, 930, 1170, 1428
BstDSI CCRYGG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 4 cut(s) 202, 625, 1265, 1346
BstFNI CGCG 2 cut(s) 191, 1051
BstKTI GATC 9 cut(s) 355, 591, 613, 625, 887, 1019, 1126, 1176, 1212
BstMAI GTCTC 3 cut(s) 104, 131, 1336
BstMBI GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 859, 1186
BstSFI CTRYAG 1 cut(s) 156
BstUI CGCG 2 cut(s) 191, 1051
BstV1I GCAGC 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 483, 931
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 591
BsuTUI ATCGAT 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 4 cut(s) 202, 625, 1265, 1346
BtsIMutI CAGTG 2 cut(s) 219, 1176
Cac8I GCNNGC 2 cut(s) 900, 1028
CaiI CAGNNNCTG 2 cut(s) 230, 1169
CciI TCATGA 1 cut(s) 1005
Cfr10I RCCGGY 1 cut(s) 1357
Cfr13I GGNCC 2 cut(s) 998, 1043
Cfr42I CCGCGG 1 cut(s) 192
ClaI ATCGAT 1 cut(s) 591
Csp6I GTAC 2 cut(s) 973, 1100
CspAI ACCGGT 1 cut(s) 1357
CviAII CATG 3 cut(s) 647, 653, 1006
CviQI GTAC 2 cut(s) 973, 1100
DdeI CTNAG 7 cut(s) 135, 512, 881, 894, 930, 1170, 1428
DpnI GATC 9 cut(s) 354, 590, 612, 624, 886, 1018, 1125, 1175, 1211
DpnII GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
Eam1104I CTCTTC 2 cut(s) 282, 1290
EarI CTCTTC 2 cut(s) 282, 1290
Eco130I CCWWGG 1 cut(s) 965
Eco31I GGTCTC 1 cut(s) 1336
Eco47I GGWCC 2 cut(s) 998, 1043
Eco57I CTGAAG 4 cut(s) 27, 93, 306, 899
Eco88I CYCGRG 1 cut(s) 1085
Eco91I GGTNACC 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 665
EcoRI GAATTC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 965
EcoT22I ATGCAT 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 965
FaeI CATG 3 cut(s) 650, 656, 1009
FatI CATG 3 cut(s) 646, 652, 1005
FblI GTMKAC 1 cut(s) 1413
Fnu4HI GCNGC 2 cut(s) 192, 340
FokI GGATG 4 cut(s) 189, 632, 1272, 1333
Fsp4HI GCNGC 2 cut(s) 192, 340
FspBI CTAG 5 cut(s) 398, 413, 519, 765, 800
GluI GCNGC 2 cut(s) 192, 340
GsuI CTGGAG 1 cut(s) 143
HapII CCGG 6 cut(s) 38, 149, 860, 1188, 1292, 1358
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 3 cut(s) 650, 656, 1009
HincII GTYRAC 1 cut(s) 1365
HindII GTYRAC 1 cut(s) 1365
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 7 cut(s) 83, 304, 313, 463, 539, 769, 1160
HpaI GTTAAC 1 cut(s) 1365
HpaII CCGG 6 cut(s) 38, 149, 860, 1188, 1292, 1358
HphI GGTGA 6 cut(s) 413, 421, 544, 659, 704, 1074
Hpy166II GTNNAC 4 cut(s) 1232, 1365, 1414, 1447
Hpy188I TCNGA 9 cut(s) 57, 73, 82, 352, 367, 702, 733, 1171, 1351
Hpy8I GTNNAC 4 cut(s) 1232, 1365, 1414, 1447
Hpy99I CGWCG 3 cut(s) 241, 494, 497
HpyAV CCTTC 4 cut(s) 60, 78, 656, 1056
HpyCH4III ACNGT 6 cut(s) 214, 991, 1180, 1204, 1315, 1411
HpyCH4IV ACGT 2 cut(s) 492, 725
HpyCH4V TGCA 4 cut(s) 158, 233, 646, 1137
HpyF3I CTNAG 7 cut(s) 135, 512, 881, 894, 930, 1170, 1428
HpySE526I ACGT 2 cut(s) 492, 725
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 3 cut(s) 650, 656, 1009
KpnI GGTACC 1 cut(s) 976
KspAI GTTAAC 1 cut(s) 1365
KspI CCGCGG 1 cut(s) 192
Kzo9I GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 5 cut(s) 85, 208, 242, 275, 1294
MaeI CTAG 5 cut(s) 398, 413, 519, 765, 800
MaeII ACGT 2 cut(s) 492, 725
MaeIII GTNAC 6 cut(s) 31, 130, 214, 665, 677, 1252
MalI GATC 9 cut(s) 354, 590, 612, 624, 886, 1018, 1125, 1175, 1211
MboI GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
MflI RGATCY 1 cut(s) 610
MluCI AATT 7 cut(s) 361, 425, 506, 596, 853, 922, 1283
MmeI TCCRAC 1 cut(s) 1134
Mph1103I ATGCAT 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 752
MseI TTAA 5 cut(s) 255, 503, 840, 1182, 1364
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 191, 1058
MspI CCGG 6 cut(s) 38, 149, 860, 1188, 1292, 1358
MspR9I CCNGG 2 cut(s) 861, 1188
MvnI CGCG 2 cut(s) 191, 1051
NciI CCSGG 2 cut(s) 861, 1188
NdeII GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
NlaIII CATG 3 cut(s) 650, 656, 1009
NlaIV GGNNCC 1 cut(s) 974
NmuCI GTSAC 3 cut(s) 130, 214, 665
NsiI ATGCAT 1 cut(s) 648
PagI TCATGA 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 7 cut(s) 83, 304, 313, 463, 539, 769, 1160
PinAI ACCGGT 1 cut(s) 1357
PkrI GCNGC 2 cut(s) 193, 341
PsiI TTATAA 1 cut(s) 717
PspEI GGTNACC 1 cut(s) 665
PspN4I GGNNCC 1 cut(s) 974
PspPI GGNCC 2 cut(s) 998, 1043
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 230, 1169
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 2 cut(s) 974, 1101
RsaNI GTAC 2 cut(s) 973, 1100
RseI CAYNNNNRTG 1 cut(s) 651
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 5 cut(s) 255, 503, 840, 1182, 1364
SatI GCNGC 2 cut(s) 192, 340
Sau3AI GATC 9 cut(s) 352, 588, 610, 622, 884, 1016, 1123, 1173, 1209
Sau96I GGNCC 2 cut(s) 998, 1043
ScrFI CCNGG 2 cut(s) 861, 1188
SfaNI GCATC 5 cut(s) 85, 208, 242, 275, 1294
SfcI CTRYAG 1 cut(s) 156
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 2 cut(s) 998, 1043
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 3 cut(s) 90, 554, 757
SmoI CTYRAG 3 cut(s) 90, 554, 757
Sse9I AATT 7 cut(s) 361, 425, 506, 596, 853, 922, 1283
SsiI CCGC 2 cut(s) 189, 191
SspMI CTAG 5 cut(s) 398, 413, 519, 765, 800
StyD4I CCNGG 2 cut(s) 859, 1186
StyI CCWWGG 1 cut(s) 965
TaaI ACNGT 6 cut(s) 214, 991, 1180, 1204, 1315, 1411
TaiI ACGT 2 cut(s) 495, 728
TaqI TCGA 5 cut(s) 236, 381, 403, 591, 603
TaqII GACCGA 1 cut(s) 672
TasI AATT 7 cut(s) 361, 425, 506, 596, 853, 922, 1283
TatI WGTACW 1 cut(s) 1099
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 7 cut(s) 83, 304, 313, 463, 539, 769, 1160
Tru1I TTAA 5 cut(s) 255, 503, 840, 1182, 1364
Tru9I TTAA 5 cut(s) 255, 503, 840, 1182, 1364
TscAI CASTG 2 cut(s) 219, 1183
TseFI GTSAC 3 cut(s) 130, 214, 665
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 3 cut(s) 130, 214, 665
TspDTI ATGAA 6 cut(s) 312, 399, 609, 863, 994, 1368
TspGWI ACGGA 3 cut(s) 233, 254, 990
TspRI CASTG 2 cut(s) 219, 1183
VpaK11BI GGWCC 2 cut(s) 998, 1043
XapI RAATTY 3 cut(s) 506, 853, 1283
XbaI TCTAGA 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 1413
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 5 cut(s) 398, 413, 519, 765, 800
ZraI GACGTC 1 cut(s) 493
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.