Rorug07G0242100

Patellin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012299
Physical Location & Seq
Forward (+)
22272351 .. 22274736
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0242100.1

Sequence Viewer

Length: 1389 bp
ATGTTTCAGGATGGCACAAAAAGTATAAAGAGCTATCTTCAGACCAATGTAGAAGCTGTGCTAAAGGATCGCCTTAATGAGATTGATGACCCGTGTGTTGTCTATATGGATGCCTCTGTGGGGACAGAGGAGTTGTCTTATTTTAGATTTGAGAAGATGAGTGCATTAGATTATGATCAGGAATTAAGGGACTTCATAAACTCTTCAAGTGAATTAAGATCAACATACAGGAAAGAGAAGGTCTGGCTGAAGAAAGTTTCCATGCTGAAACGAAGTTTTCCTGAATTTCTGGCTAATGAAAAGGCGGTGAAGGATCCGGCCAAAGTTTCTAAGGTTCTGCATATAATAGACAGTCTATTAGAAAATGCAACTCATGAAAATGCCACAGTGAATGCTTTTGGCTCCTTGGAGAAATCTGTTGAACCTCGGGATGATCAATTGGGGAGATCTAAGAAGATGGCTAAGAATGGTATCAATGGTCTGCGAGCATCTTTGTCCAATCTGATGGTAAAGGACGAATTCATTGGTACAGTAGCTGCAGCCTTTCTCATGTCTATTGATTCTCCACCACAGGGTCTCCCATGTAGACCTGCCAAGACATTTTTACTGTTGGGTCTTACGGAAGTTGGTAAGGCAGATCTTGTTAAAGGTTTAATGGAGCATTTTGTGAGTGATGATGGGATGAGCCTATTGTTTCAAATTGATATGTCCAAGCATGATGACACAGAGACATGTTTCTCAAGCATGTACACTAGCAATGAGCAAGCGGTGAAGATAGTGCCTTATAGTATTTTCCTTATTGATCGAGTTGAAGATGCCCTTCCCTCCGAGTTCAATGCTCTAATACAACTGCTTGACTGCTGTACAATGAGTGACCAGCATGGCCGCAAGGTTATTTTTAGTTGCTGCATTGTCATGGTTGCTTCAAGCGCTGGGAACAAGGACTTCATTGAAAAACTTGTCCACAGGAAATATAGTGCCAGATTTAATGGGAATGTCCTTGGACAGGAACTAAAGACATTTAGGTTCGAATTGCTGAATCGAATGGATGAGATGGTACTATTCAGTTCCTTATTTGGTGATCAACTTGGAAAATTCCTAAGGCTGTCAATGGATGGTGGAGGTGATCTTCATGAGAATCCTGTAAAGGAGGCTCTTGCATTTGAACTTGCTTTTCAGGATTTGTTTCTGCCTGCTAAATCTCTACCTGATCCACCCAGTCCACAGAACCAAGCAGATTCTGTGTTCTATCTCTTAAAATGGCGTGTCGTCAGAGACGGTGGACTTGAAGTTATGGACAAGAGTACCTTTTATCGCCACTTCTTGAATGGTTCATCAGGGAATATGAATGAGTCTGATACAGGACCAGTAATTCTATTGTCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

462

Amino Acids

51.85

Weight (kDa)

5.31

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 197 - 351 3e-14 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72160
fragaria_vesca FvH4_5g31710 FvH4_5g31710
malus_domestica MD08G1218000.v1.1 MD15G1405900.v1.1
prunus_persica Prupe.1G555300_v2.0.a1
pyrus_communis pycom08g18900 pycom15g36270
rosa_chinensis RchiOBHm_Chr7g0227591
rosa_laevigata RLG00000001634
rosa_multiflora Rmu_sc0001772.1_g000008
rosa_roxburghii Rroxscaffold_3G00232020
rosa_rugosa Rorug07G0242000 Rorug07G0242100
rosa_samantha Rh7AG395500 Rh7BG377300 Rh7CG414200 Rh7DG391300
rosa_wichuraiana Rw7G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 586
AciI CCGC 3 cut(s) 305, 767, 886
AclWI GGATC 4 cut(s) 75, 308, 321, 1205
AcoI YGGCCR 2 cut(s) 318, 883
AcsI RAATTY 3 cut(s) 284, 518, 1094
AcuI CTGAAG 2 cut(s) 23, 269
AfaI GTAC 5 cut(s) 529, 749, 865, 1059, 1306
AfeI AGCGCT 1 cut(s) 931
AfiI CCNNNNNNNGG 3 cut(s) 120, 572, 1006
AflIII ACRYGT 1 cut(s) 731
AluBI AGCT 3 cut(s) 33, 56, 536
AluI AGCT 3 cut(s) 33, 56, 536
Alw26I GTCTC 3 cut(s) 581, 722, 1269
AlwI GGATC 4 cut(s) 75, 308, 321, 1205
AlwNI CAGNNNCTG 2 cut(s) 536, 1241
Ama87I CYCGRG 1 cut(s) 426
Aor51HI AGCGCT 1 cut(s) 931
AoxI GGCC 2 cut(s) 318, 883
ApeKI GCWGC 3 cut(s) 536, 539, 906
ApoI RAATTY 3 cut(s) 284, 518, 1094
AspLEI GCGC 1 cut(s) 932
AspS9I GGNCC 1 cut(s) 1364
AsuHPI GGTGA 4 cut(s) 319, 781, 1091, 1136
AsuII TTCGAA 1 cut(s) 1029
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 1364
AxyI CCTNAGG 1 cut(s) 1100
BaeI ACNNNNGTAYC 2 cut(s) 1288, 1321
BamHI GGATCC 1 cut(s) 313
BbvI GCAGC 3 cut(s) 523, 551, 893
BccI CCATC 6 cut(s) 5, 451, 499, 671, 1048, 1109
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BclI TGATCA 3 cut(s) 175, 433, 1081
BcoDI GTCTC 3 cut(s) 581, 722, 1269
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 2 cut(s) 537, 1385
BfoI RGCGCY 1 cut(s) 933
BfuAI ACCTGC 1 cut(s) 598
BglII AGATCT 2 cut(s) 446, 637
BisI GCNGC 4 cut(s) 537, 540, 886, 907
BlsI GCNGC 4 cut(s) 538, 541, 887, 908
Bme18I GGWCC 1 cut(s) 1364
BmeT110I CYCGRG 1 cut(s) 426
BmgT120I GGNCC 1 cut(s) 1364
BmiI GGNNCC 2 cut(s) 315, 403
BmrI ACTGGG 1 cut(s) 1212
BmsI GCATC 3 cut(s) 100, 497, 805
BmuI ACTGGG 1 cut(s) 1212
Bpu14I TTCGAA 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 724
BsaBI GATNNNNATC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 3 cut(s) 405, 425, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 572, 1006
Bse1I ACTGG 2 cut(s) 1218, 1367
Bse21I CCTNAGG 1 cut(s) 1100
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 405, 425, 1000
BseGI GGATG 6 cut(s) 16, 115, 436, 687, 1054, 1120
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 3 cut(s) 120, 572, 1006
BseMI GCAATG 1 cut(s) 763
BseNI ACTGG 2 cut(s) 1218, 1367
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 3 cut(s) 523, 551, 893
BseYI CCCAGC 1 cut(s) 932
BshFI GGCC 2 cut(s) 320, 885
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 2 cut(s) 136, 203
BslI CCNNNNNNNGG 3 cut(s) 120, 572, 1006
BsmAI GTCTC 3 cut(s) 581, 722, 1269
BsmBI CGTCTC 1 cut(s) 1269
BsmFI GGGAC 2 cut(s) 136, 203
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 320, 885
Bso31I GGTCTC 1 cut(s) 581
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 1029
Bsp1407I TGTACA 2 cut(s) 747, 863
BspACI CCGC 3 cut(s) 305, 767, 886
BspANI GGCC 2 cut(s) 320, 885
BspHI TCATGA 2 cut(s) 373, 1132
BspLI GGNNCC 2 cut(s) 315, 403
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 4 cut(s) 75, 308, 321, 1205
BspT104I TTCGAA 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 763
BsrGI TGTACA 2 cut(s) 747, 863
BsrI ACTGG 2 cut(s) 1218, 1367
BssECI CCNNGG 3 cut(s) 405, 425, 1000
BssT1I CCWWGG 2 cut(s) 405, 1000
Bst4CI ACNGT 5 cut(s) 353, 388, 532, 609, 1280
Bst6I CTCTTC 1 cut(s) 208
BstAUI TGTACA 2 cut(s) 747, 863
BstBI TTCGAA 1 cut(s) 1029
BstC8I GCNNGC 3 cut(s) 486, 765, 1194
BstDEI CTNAG 4 cut(s) 330, 450, 462, 1100
BstENI CCTNNNNNAGG 1 cut(s) 1004
BstF5I GGATG 6 cut(s) 16, 115, 436, 687, 1054, 1120
BstH2I RGCGCY 1 cut(s) 933
BstHHI GCGC 1 cut(s) 932
BstMAI GTCTC 3 cut(s) 581, 722, 1269
BstMWI GCNNNNNNNGC 1 cut(s) 929
BstNSI RCATGY 2 cut(s) 735, 748
BstSFI CTRYAG 2 cut(s) 537, 1385
BstV1I GCAGC 3 cut(s) 523, 551, 893
BstX2I RGATCY 3 cut(s) 313, 446, 637
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 3 cut(s) 313, 446, 637
Bsu36I CCTNAGG 1 cut(s) 1100
BsuRI GGCC 2 cut(s) 320, 885
BtsCI GGATG 6 cut(s) 16, 115, 436, 687, 1054, 1120
BtsIMutI CAGTG 1 cut(s) 393
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 3 cut(s) 486, 765, 1194
CaiI CAGNNNCTG 2 cut(s) 536, 1241
CciI TCATGA 2 cut(s) 373, 1132
CfoI GCGC 1 cut(s) 932
Cfr13I GGNCC 1 cut(s) 1364
Csp6I GTAC 5 cut(s) 528, 748, 864, 1058, 1305
CviQI GTAC 5 cut(s) 528, 748, 864, 1058, 1305
DdeI CTNAG 4 cut(s) 330, 450, 462, 1100
EaeI YGGCCR 2 cut(s) 318, 883
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco130I CCWWGG 2 cut(s) 405, 1000
Eco31I GGTCTC 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 1364
Eco47III AGCGCT 1 cut(s) 931
Eco57I CTGAAG 2 cut(s) 23, 269
Eco81I CCTNAGG 1 cut(s) 1100
Eco88I CYCGRG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 1004
EcoRI GAATTC 1 cut(s) 518
EcoT14I CCWWGG 2 cut(s) 405, 1000
ErhI CCWWGG 2 cut(s) 405, 1000
Esp3I CGTCTC 1 cut(s) 1269
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 1292, 1324
FaqI GGGAC 2 cut(s) 136, 203
FbaI TGATCA 3 cut(s) 175, 433, 1081
FblI GTMKAC 1 cut(s) 586
Fnu4HI GCNGC 4 cut(s) 537, 540, 886, 907
FokI GGATG 6 cut(s) 23, 122, 443, 694, 1061, 1127
Fsp4HI GCNGC 4 cut(s) 537, 540, 886, 907
FspBI CTAG 1 cut(s) 753
GlaI GCGC 1 cut(s) 931
GluI GCNGC 4 cut(s) 537, 540, 886, 907
GsaI CCCAGC 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 933
HaeIII GGCC 2 cut(s) 320, 885
HapII CCGG 1 cut(s) 317
HhaI GCGC 1 cut(s) 932
Hin6I GCGC 1 cut(s) 930
HinP1I GCGC 1 cut(s) 930
HinfI GANTC 5 cut(s) 560, 1039, 1138, 1238, 1352
HpaII CCGG 1 cut(s) 317
HphI GGTGA 4 cut(s) 319, 781, 1091, 1136
Hpy166II GTNNAC 5 cut(s) 587, 750, 964, 1223, 1283
Hpy188I TCNGA 5 cut(s) 42, 504, 829, 1274, 1357
Hpy188III TCNNGA 8 cut(s) 8, 179, 281, 374, 428, 1133, 1178, 1324
Hpy8I GTNNAC 5 cut(s) 587, 750, 964, 1223, 1283
HpyAV CCTTC 3 cut(s) 232, 304, 830
HpyCH4III ACNGT 5 cut(s) 353, 388, 532, 609, 1280
HpyCH4V TGCA 6 cut(s) 164, 340, 368, 539, 909, 1160
HpyF10VI GCNNNNNNNGC 1 cut(s) 929
HpyF3I CTNAG 4 cut(s) 330, 450, 462, 1100
HspAI GCGC 1 cut(s) 930
Ksp22I TGATCA 3 cut(s) 175, 433, 1081
LmnI GCTCC 2 cut(s) 407, 658
Lsp1109I GCAGC 3 cut(s) 523, 551, 893
LweI GCATC 3 cut(s) 100, 497, 805
MaeI CTAG 1 cut(s) 753
MaeIII GTNAC 2 cut(s) 872, 1380
MboII GAAGA 8 cut(s) 29, 166, 195, 262, 466, 784, 824, 1121
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 3 cut(s) 313, 446, 637
MluCI AATT 9 cut(s) 182, 212, 284, 437, 518, 699, 1031, 1094, 1371
MlyI GAGTC 1 cut(s) 1361
MnlI CCTC 6 cut(s) 121, 124, 435, 835, 1115, 1144
MseI TTAA 7 cut(s) 75, 185, 215, 645, 653, 987, 1256
MslI CAYNNNNRTG 2 cut(s) 378, 914
MspI CCGG 1 cut(s) 317
MunI CAATTG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 1 cut(s) 929
NlaIV GGNNCC 2 cut(s) 315, 403
NmuCI GTSAC 2 cut(s) 872, 1380
NspI RCATGY 2 cut(s) 735, 748
NspV TTCGAA 1 cut(s) 1029
PagI TCATGA 2 cut(s) 373, 1132
PciI ACATGT 1 cut(s) 731
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 4 cut(s) 560, 1039, 1138, 1238
PkrI GCNGC 4 cut(s) 538, 541, 887, 908
PleI GAGTC 1 cut(s) 1360
PpsI GAGTC 1 cut(s) 1360
PscI ACATGT 1 cut(s) 731
PspFI CCCAGC 1 cut(s) 932
PspN4I GGNNCC 2 cut(s) 315, 403
PspPI GGNCC 1 cut(s) 1364
PstI CTGCAG 1 cut(s) 541
PstNI CAGNNNCTG 2 cut(s) 536, 1241
PsuI RGATCY 3 cut(s) 313, 446, 637
RsaI GTAC 5 cut(s) 529, 749, 865, 1059, 1306
RsaNI GTAC 5 cut(s) 528, 748, 864, 1058, 1305
RseI CAYNNNNRTG 2 cut(s) 378, 914
SaqAI TTAA 7 cut(s) 75, 185, 215, 645, 653, 987, 1256
SatI GCNGC 4 cut(s) 537, 540, 886, 907
Sau96I GGNCC 1 cut(s) 1364
SchI GAGTC 1 cut(s) 1361
SfaNI GCATC 3 cut(s) 100, 497, 805
SfcI CTRYAG 2 cut(s) 537, 1385
SfuI TTCGAA 1 cut(s) 1029
SinI GGWCC 1 cut(s) 1364
SmiMI CAYNNNNRTG 2 cut(s) 378, 914
SmlI CTYRAG 1 cut(s) 739
SmoI CTYRAG 1 cut(s) 739
Sse9I AATT 9 cut(s) 182, 212, 284, 437, 518, 699, 1031, 1094, 1371
SsiI CCGC 3 cut(s) 305, 767, 886
SspMI CTAG 1 cut(s) 753
StyI CCWWGG 2 cut(s) 405, 1000
TaaI ACNGT 5 cut(s) 353, 388, 532, 609, 1280
TaqI TCGA 3 cut(s) 805, 1029, 1042
TasI AATT 9 cut(s) 182, 212, 284, 437, 518, 699, 1031, 1094, 1371
TatI WGTACW 2 cut(s) 747, 863
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 4 cut(s) 560, 1039, 1138, 1238
Tru1I TTAA 7 cut(s) 75, 185, 215, 645, 653, 987, 1256
Tru9I TTAA 7 cut(s) 75, 185, 215, 645, 653, 987, 1256
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 2 cut(s) 872, 1380
TseI GCWGC 3 cut(s) 536, 539, 906
Tsp45I GTSAC 2 cut(s) 872, 1380
TspDTI ATGAA 8 cut(s) 184, 312, 390, 511, 937, 1121, 1323, 1361
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 1364
XagI CCTNNNNNAGG 1 cut(s) 1004
XapI RAATTY 3 cut(s) 284, 518, 1094
XceI RCATGY 2 cut(s) 735, 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 1325
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.