Rh7AG395500

Patellin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012299
Physical Location & Seq
Forward (+)
51798400 .. 51801870
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG395500.1

Sequence Viewer

Length: 1260 bp
ATGGCTGATGAAACCACCATAACCACCCCGCCACTGGTAGACCAACCACCTTTCGCGGTGGTTGAGTCCGAATCGCCCTCTGTAGCCGAAAAACTCGAGTCTAAAGAAGACCCACTTCCACCAGCGCCATTACCAGAAGTGACAGAGTCTGCCAAGGAAGAAGAGCCACCGCTAGAGCTGCCTTACGAGTCTCCTCCTGAGGTGAAGGCTAAATCCGAAGAGGAGGTTTTAGTTGTTGGGTCTGTGCAGGCCAAGGAGGGTGAGGAGCCAAAGATTCGGAAACCGATTCCGCAGTCTCTGGTTTCGTTCAAAGAGGAAAGCAATATAGTGGCGGATCTCTCAGATTCCGAGAGGAAAGCACTTCAAGAACTGAAGCAGCTCGTCCAGGAAGCTCTCAACACTCGCCAGTTCACTTCCCCAAAACAAGAAGTGAAAGAGACCAAGGACAAACCAGCTCCCAAAACTGAAGAAGACACTGCTGGTTCGGAATCCGAAACAAAGCCACTCCATGAGCCCAAAATCGAGGAGAACCCAGTGAAGGAAGCTGAGGAAGTAGCTAAGGAAACATCGCAACTGGCGATTTCTCCTGAGGAGGTTTCAATTTGGGGTGTTCCGCTGCTCAAGGACGACAGGAGCGATGTGATTCTTCTCAAGTTCCTTAGGGCTCGGGACTTCAAGGTGAGAGATGCTTTTGTTATGCTCCGAAACACGATCCAGTGGAGGAAGAAGTTTGGGATCGATGCTCTTGTGGACGAAGATCTTGGTGATGATTTGGAGAAAGTGGTTTTTATGCATGGGTATGACAGAGAGGGTCACCCTGTGTGTTACAATGTGTTTGGGGAGTTTCAGAACAAGGAGCTGTATCAGAAGACGTTTTTGGATGAGGAAAAGAGAACCAAGTTTCTCCGGTGGCGGATTCAGTTCTTGGAGAGAAGCATTAGGAAGCTGGATTTCAGCCCTGGTGGTGTTTGCACTATTTTTCAGGTTAATGATCTTAAGAATTCTCCTGGCCCTGCAAAGAGTGAGCTTCGCTTGGCCACCAAACAAGCCCTGCAGTTGCTTCAGGACAATTATCCCGAGTTTGTAGTCAAACAGGTGTTTGTCAATGCTCCTTGGTGGTATCTTGCGTTCTACACATTGATCAGTCCCTTCATGACACAGAGGACCAAAAGCAAGTTTGTCTTTGCAAGCCCAGCCAAATCTGCAGAGACCCTTTTCAAGTTAGTAGAGTTTCTGTCCATCTTACGTTGCATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

419

Amino Acids

47.79

Weight (kDa)

4.98

Isoelectric Point (pI)

63.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 185 - 236 8.7e-10 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 260 - 409 6.5e-26 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72160
fragaria_vesca FvH4_5g31710 FvH4_5g31710
malus_domestica MD08G1218000.v1.1 MD15G1405900.v1.1
prunus_persica Prupe.1G555300_v2.0.a1
pyrus_communis pycom08g18900 pycom15g36270
rosa_chinensis RchiOBHm_Chr7g0227591
rosa_laevigata RLG00000001634
rosa_multiflora Rmu_sc0001772.1_g000008
rosa_roxburghii Rroxscaffold_3G00232020
rosa_rugosa Rorug07G0242000 Rorug07G0242100
rosa_samantha Rh7AG395500 Rh7BG377300 Rh7CG414200 Rh7DG391300
rosa_wichuraiana Rw7G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 39
AccII CGCG 1 cut(s) 56
AciI CCGC 7 cut(s) 29, 56, 170, 290, 332, 614, 913
AclWI GGATC 3 cut(s) 342, 706, 743
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 1 cut(s) 1000
AcuI CTGAAG 3 cut(s) 392, 486, 1046
AdeI CACNNNGTG 1 cut(s) 820
AfiI CCNNNNNNNGG 2 cut(s) 34, 538
AflII CTTAAG 1 cut(s) 995
AgsI TTSAA 5 cut(s) 310, 365, 600, 676, 1219
AjnI CCWGG 3 cut(s) 384, 958, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 9 cut(s) 178, 379, 392, 455, 545, 557, 859, 946, 1027
AluI AGCT 9 cut(s) 178, 379, 392, 455, 545, 557, 859, 946, 1027
Alw26I GTCTC 4 cut(s) 195, 300, 431, 1202
AlwI GGATC 3 cut(s) 342, 706, 743
AlwNI CAGNNNCTG 2 cut(s) 149, 298
Ama87I CYCGRG 3 cut(s) 95, 666, 1076
AoxI GGCC 3 cut(s) 249, 1009, 1035
ApeKI GCWGC 3 cut(s) 178, 376, 616
ApoI RAATTY 1 cut(s) 1000
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 1010, 1164
AsuHPI GGTGA 5 cut(s) 214, 272, 691, 776, 806
AvaI CYCGRG 3 cut(s) 95, 666, 1076
AvaII GGWCC 1 cut(s) 1164
AxyI CCTNAGG 3 cut(s) 198, 588, 659
BalI TGGCCA 1 cut(s) 1037
BanII GRGCYC 2 cut(s) 516, 667
BbsI GAAGAC 3 cut(s) 114, 477, 875
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 165, 388, 603
BccI CCATC 1 cut(s) 1247
BciT130I CCWGG 3 cut(s) 386, 960, 1008
BclI TGATCA 1 cut(s) 1140
BcoDI GTCTC 4 cut(s) 195, 300, 431, 1202
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 3 cut(s) 81, 1052, 1203
BfoI RGCGCY 1 cut(s) 128
BfrI CTTAAG 1 cut(s) 995
BglII AGATCT 1 cut(s) 757
BisI GCNGC 3 cut(s) 179, 377, 617
BlsI GCNGC 3 cut(s) 180, 378, 618
Bme1390I CCNGG 3 cut(s) 386, 960, 1008
Bme18I GGWCC 1 cut(s) 1164
BmeT110I CYCGRG 3 cut(s) 95, 666, 1076
BmgT120I GGNCC 2 cut(s) 1010, 1164
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 3 cut(s) 386, 960, 1008
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 2 cut(s) 676, 730
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 3 cut(s) 114, 477, 875
Bpu10I CCTNAGC 2 cut(s) 546, 558
BpuEI CTTGAG 2 cut(s) 605, 635
Bsa29I ATCGAT 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 431, 1202
BsaJI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BsaWI WCCGGW 1 cut(s) 906
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 538
Bse1I ACTGG 5 cut(s) 39, 406, 533, 579, 715
Bse21I CCTNAGG 3 cut(s) 198, 588, 659
BseBI CCWGG 3 cut(s) 386, 960, 1008
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BseGI GGATG 1 cut(s) 886
BseLI CCNNNNNNNGG 2 cut(s) 34, 538
BseMII CTCAG 4 cut(s) 189, 354, 537, 579
BseNI ACTGG 5 cut(s) 39, 406, 533, 579, 715
BseRI GAGGAG 5 cut(s) 183, 236, 278, 539, 605
BseXI GCAGC 3 cut(s) 165, 388, 603
BseYI CCCAGC 1 cut(s) 1192
BsgI GTGCAG 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 251, 1011, 1037
BshVI ATCGAT 1 cut(s) 738
BsiHKCI CYCGRG 3 cut(s) 95, 666, 1076
BsiSI CCGG 1 cut(s) 907
BslFI GGGAC 2 cut(s) 683, 1131
BslI CCNNNNNNNGG 2 cut(s) 34, 538
BsmAI GTCTC 4 cut(s) 195, 300, 431, 1202
BsmFI GGGAC 2 cut(s) 683, 1131
BsnI GGCC 3 cut(s) 251, 1011, 1037
Bso31I GGTCTC 2 cut(s) 431, 1202
BsoBI CYCGRG 3 cut(s) 95, 666, 1076
Bsp1286I GDGCHC 2 cut(s) 516, 667
Bsp143I GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
BspACI CCGC 7 cut(s) 29, 56, 170, 290, 332, 614, 913
BspANI GGCC 3 cut(s) 251, 1011, 1037
BspCNI CTCAG 4 cut(s) 190, 353, 538, 580
BspDI ATCGAT 1 cut(s) 738
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 2 cut(s) 1056, 1207
BspPI GGATC 3 cut(s) 342, 706, 743
BspQI GCTCTTC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 995
BspTNI GGTCTC 2 cut(s) 431, 1202
BsrI ACTGG 5 cut(s) 39, 406, 533, 579, 715
BssECI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BssMI GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
BssT1I CCWWGG 4 cut(s) 153, 252, 441, 1112
Bst2UI CCWGG 3 cut(s) 386, 960, 1008
Bst6I CTCTTC 2 cut(s) 156, 213
BstAFI CTTAAG 1 cut(s) 995
BstC8I GCNNGC 2 cut(s) 249, 1189
BstDEI CTNAG 6 cut(s) 198, 340, 546, 558, 588, 659
BstEII GGTNACC 1 cut(s) 812
BstF5I GGATG 1 cut(s) 886
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 6 cut(s) 337, 714, 738, 760, 994, 1143
BstMAI GTCTC 4 cut(s) 195, 300, 431, 1202
BstMBI GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 178, 1193, 1202
BstNI CCWGG 3 cut(s) 386, 960, 1008
BstPI GGTNACC 1 cut(s) 812
BstSCI CCNGG 3 cut(s) 384, 958, 1006
BstSFI CTRYAG 3 cut(s) 81, 1052, 1203
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 3 cut(s) 165, 388, 603
BstV2I GAAGAC 3 cut(s) 114, 477, 875
BstX2I RGATCY 2 cut(s) 334, 757
BstYI RGATCY 2 cut(s) 334, 757
Bsu15I ATCGAT 1 cut(s) 738
Bsu36I CCTNAGG 3 cut(s) 198, 588, 659
BsuRI GGCC 3 cut(s) 251, 1011, 1037
BsuTUI ATCGAT 1 cut(s) 738
BtgZI GCGATG 2 cut(s) 552, 651
BtsCI GGATG 1 cut(s) 886
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 4 cut(s) 32, 474, 540, 722
Cac8I GCNNGC 2 cut(s) 249, 1189
CaiI CAGNNNCTG 2 cut(s) 149, 298
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 1010, 1164
ClaI ATCGAT 1 cut(s) 738
CviAII CATG 4 cut(s) 509, 794, 1153, 1252
DdeI CTNAG 6 cut(s) 198, 340, 546, 558, 588, 659
DpnI GATC 6 cut(s) 336, 713, 737, 759, 993, 1142
DpnII GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
DraIII CACNNNGTG 1 cut(s) 820
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 2 cut(s) 156, 213
EarI CTCTTC 2 cut(s) 156, 213
EciI GGCGGA 2 cut(s) 347, 928
Eco130I CCWWGG 4 cut(s) 153, 252, 441, 1112
Eco24I GRGCYC 2 cut(s) 516, 667
Eco31I GGTCTC 2 cut(s) 431, 1202
Eco47I GGWCC 1 cut(s) 1164
Eco57I CTGAAG 3 cut(s) 392, 486, 1046
Eco81I CCTNAGG 3 cut(s) 198, 588, 659
Eco88I CYCGRG 3 cut(s) 95, 666, 1076
Eco91I GGTNACC 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 384, 958, 1006
EcoT14I CCWWGG 4 cut(s) 153, 252, 441, 1112
EcoT22I ATGCAT 1 cut(s) 795
EcoT38I GRGCYC 2 cut(s) 516, 667
ErhI CCWWGG 4 cut(s) 153, 252, 441, 1112
FaeI CATG 4 cut(s) 512, 797, 1156, 1255
FaiI YATR 9 cut(s) 20, 326, 510, 698, 791, 795, 801, 1154, 1253
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 1166, 1198
FaqI GGGAC 2 cut(s) 683, 1131
FatI CATG 4 cut(s) 508, 793, 1152, 1251
FauI CCCGC 1 cut(s) 36
FbaI TGATCA 1 cut(s) 1140
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 179, 377, 617
FokI GGATG 1 cut(s) 893
FriOI GRGCYC 2 cut(s) 516, 667
Fsp4HI GCNGC 3 cut(s) 179, 377, 617
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 126
GluI GCNGC 3 cut(s) 179, 377, 617
GsaI CCCAGC 1 cut(s) 1196
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 3 cut(s) 251, 1011, 1037
HapII CCGG 1 cut(s) 907
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 512, 797, 1156, 1255
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HpaII CCGG 1 cut(s) 907
HphI GGTGA 5 cut(s) 214, 272, 691, 776, 806
Hpy166II GTNNAC 3 cut(s) 40, 411, 751
Hpy188III TCNNGA 7 cut(s) 197, 365, 587, 668, 1064, 1076, 1153
Hpy8I GTNNAC 3 cut(s) 40, 411, 751
HpyAV CCTTC 3 cut(s) 199, 532, 1159
HpyCH4IV ACGT 2 cut(s) 872, 1246
HpyCH4V TGCA 8 cut(s) 247, 793, 972, 1016, 1054, 1187, 1205, 1251
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 1193, 1202
HpyF3I CTNAG 6 cut(s) 198, 340, 546, 558, 588, 659
HpySE526I ACGT 2 cut(s) 872, 1246
Hsp92II CATG 4 cut(s) 512, 797, 1156, 1255
HspAI GCGC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 1140
Kzo9I GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 6 cut(s) 265, 460, 633, 705, 856, 1114
Lsp1109I GCAGC 3 cut(s) 165, 388, 603
LweI GCATC 2 cut(s) 676, 730
MaeI CTAG 1 cut(s) 173
MaeII ACGT 2 cut(s) 872, 1246
MaeIII GTNAC 3 cut(s) 139, 812, 824
MalI GATC 6 cut(s) 336, 713, 737, 759, 993, 1142
MboI GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
MflI RGATCY 2 cut(s) 334, 757
MhlI GDGCHC 2 cut(s) 516, 667
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 3 cut(s) 600, 1000, 1069
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 4 cut(s) 74, 107, 155, 197
Mox20I TGGCCA 1 cut(s) 1037
Mph1103I ATGCAT 1 cut(s) 795
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 2 cut(s) 987, 996
MslI CAYNNNNRTG 1 cut(s) 798
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 1 cut(s) 616
MspCI CTTAAG 1 cut(s) 995
MspI CCGG 1 cut(s) 907
MspR9I CCNGG 3 cut(s) 386, 960, 1008
MvaI CCWGG 3 cut(s) 386, 960, 1008
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 178, 1193, 1202
NdeII GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
NlaIII CATG 4 cut(s) 512, 797, 1156, 1255
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 139, 812
NsiI ATGCAT 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 95
PagI TCATGA 1 cut(s) 1152
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 575, 633
PfeI GAWTC 7 cut(s) 71, 274, 286, 344, 488, 643, 916
PflFI GACNNNGTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 3 cut(s) 180, 378, 618
PleI GAGTC 4 cut(s) 73, 106, 154, 196
PpsI GAGTC 4 cut(s) 73, 106, 154, 196
Psp6I CCWGG 3 cut(s) 384, 958, 1006
PspEI GGTNACC 1 cut(s) 812
PspFI CCCAGC 1 cut(s) 1192
PspGI CCWGG 3 cut(s) 384, 958, 1006
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 2 cut(s) 1010, 1164
PspXI VCTCGAGB 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 1112, 1144
PstI CTGCAG 2 cut(s) 1056, 1207
PstNI CAGNNNCTG 2 cut(s) 149, 298
PsuI RGATCY 2 cut(s) 334, 757
PsyI GACNNNGTC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 798
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 2 cut(s) 987, 996
SatI GCNGC 3 cut(s) 179, 377, 617
Sau3AI GATC 6 cut(s) 334, 711, 735, 757, 991, 1140
Sau96I GGNCC 2 cut(s) 1010, 1164
SchI GAGTC 4 cut(s) 74, 107, 155, 197
ScrFI CCNGG 3 cut(s) 386, 960, 1008
SduI GDGCHC 2 cut(s) 516, 667
SfaNI GCATC 2 cut(s) 676, 730
SfcI CTRYAG 3 cut(s) 81, 1052, 1203
Sfr274I CTCGAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 1164
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 798
SmlI CTYRAG 4 cut(s) 95, 620, 650, 995
SmoI CTYRAG 4 cut(s) 95, 620, 650, 995
Sse9I AATT 3 cut(s) 600, 1000, 1069
SsiI CCGC 7 cut(s) 29, 56, 170, 290, 332, 614, 913
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 384, 958, 1006
StyI CCWWGG 4 cut(s) 153, 252, 441, 1112
TaiI ACGT 2 cut(s) 875, 1249
TaqI TCGA 3 cut(s) 96, 522, 738
TasI AATT 3 cut(s) 600, 1000, 1069
TfiI GAWTC 7 cut(s) 71, 274, 286, 344, 488, 643, 916
Tru1I TTAA 2 cut(s) 987, 996
Tru9I TTAA 2 cut(s) 987, 996
TscAI CASTG 4 cut(s) 39, 481, 540, 722
TseFI GTSAC 2 cut(s) 139, 812
TseI GCWGC 3 cut(s) 178, 376, 616
Tsp45I GTSAC 2 cut(s) 139, 812
TspDTI ATGAA 2 cut(s) 24, 1141
TspRI CASTG 4 cut(s) 39, 481, 540, 722
Tth111I GACNNNGTC 1 cut(s) 145
Vha464I CTTAAG 1 cut(s) 995
VpaK11BI GGWCC 1 cut(s) 1164
XapI RAATTY 1 cut(s) 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.